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pAAV-Ef1a-CoffFon-BFP-WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaagctttgcaaagatggataaagggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcgaaacgtttctacctatttcEagIMluINotISbfIPvuIIL-ITREF-1a20406080100120140160
    ttttaaacagagaggaatctttgcagctaatggaccttctaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaaatttgtctctccttagaaacgtcgattacctggaagatccagaactttcctcacccttaaccgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttggccacggatctcttccaccgcgccccatttgacccAgeIMfeIEcoNIEF-1a180200220240260280300320340
    aaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgcctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtccattcacggcacacaccaagggcgcccggaccggagaaatgcccaataccgggaacgcacggEF-1a360380400420440460480500520
    ttgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtcaacttaatgaaggtgaccgacgtcatgcactaagaactagggctcgaagcccaaccttcacccaccctctcaagctccggaacgcgaattcctcggggaagcggagcacgaactcaactccggaccggacccgcgaccccggcggcgcacgcttagaccaccgtggaagcgcggacagAflIIEF-1a540560580600620640660680700
    tcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcgagcgacgaaagctattcagagatcggtaaattttaaaaactactggacgacgctgcgaaaaaaagaccgttctatcagaacatttacgcccggttctagacgtgtgaccataaagccaaaaaccccggcgcccgccgctgccccgggcacgcagggtcgcgtgtacaagccgctccgcApaIPciIBfuAIBglIIBspMIPspOMIEF-1a720740760780800820840860880
    gggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagccccggacgctcgcgccggtggctcttagcctgcccccatcagagttcgaccggccggacgagaccacggaccggagcgcggcggcacatagcggggcgggacccgccgttccgaccgggccagccgtggtcaacgcactcgcctttctaccggcgaagggccgggacgacgtccctcgFseIPasIEcoNIBstAPIEco53kIEF-1a9009209409609801,0001,0201,0401,060
    tcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggagttttacctcctgcgccgcgagccctctcgcccgcccactcagtgggtgtgtttccttttcccggaaaggcaggagtcggcagcgaagtacactgaggtgcctcatggcccgcggcaggtccgtggagctaatcaagagctcgaaaacctcatgcagcagaaatccaacccccctccKasINarISacISfoITliIXhoIBbvCIPluTIBpu10IPaeR7IEF-1a1,0801,1001,1201,1401,1601,1801,2001,2201,240
    ggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgaggatcccaaaatacgctacctcaaaggggtgtgactcacccacctctgacttcaatccggtcgaaccgtgaactacattaagaggaaccttaaacgggaaaaactcaaacctagaaccaagtaagagttcggagtctgtcaccaagtttcaaaaaaagaaggtaaagtccacagcactcctagBamHIEF-1a1,2601,2801,3001,3201,3401,3601,3801,4001,420
    cataacttcgtataggatactttatacgaagttatgtagctggagcaatttgaaaagattgttgtattacctctagctgcgcttaataacttcgtatagtataccttatacgaagttatactagtgccaccatgagcgagctgattaaggagaacatgcacatgaagctgtacatggagtattgaagcatatcctatgaaatatgcttcaatacatcgacctcgttaaacttttctaacaacataatggagatcgacgcgaattattgaagcatatcatatggaatatgcttcaatatgatcacggtggtactcgctcgactaattcctcttgtacgtgtacttcgacatgtacctSpeIBsrGIBstZ17IBFP Exon 1lox2722loxN1,4401,4601,4801,5001,5201,5401,5601,5801,600
    gggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccgtgagcccgtggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggcactcAleIBFP Exon 11,6201,6401,6601,6801,7001,7201,7401,7601,780
    tacaggagggctagcataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagataggtgcaaagaataacttcgtataaggtatactatacgaagttatggtaccgaagttcctattcttcaaatagtataggaacttcaattgatgtcctcccgatcgtattgaagcatatttcataggatatgcttcaataaacggaattgggtctttaatagtgacaataagaaatctatccacgtttcttattgaagcatattccatatgatatgcttcaataccatggcttcaaggataagaagtttatcatatccttgaagttaacBaeIBmtIKpnIMfeINheIAcc65IBstZ17Ilox 2722 Revlox N RevF31,8001,8201,8401,8601,8801,9001,9201,940
    ttattggtgcaaagaatcacttccttaagtctttatactgcccaggaagttcctattcttcaaaaggtataggaacttcggcgcgccttaattaagcttgtgccccagtttgctagggaggtcgcagtatctggccactgccacctcgtgctgctcgacgtaggtctcgttgttggccaataaccacgtttcttagtgaaggaattcagaaatatgacgggtccttcaaggataagaagttttccatatccttgaagccgcgcggaattaattcgaacacggggtcaaacgatccctccagcgtcatagaccggtgacggtggagcacgacgagctgcatccagagcaacaaccggAscIPacIAflIIAlwNIPshAIBFP Exon 2F51,9601,9802,0002,0202,0402,0602,0802,1002,120
    tccttgattctttccagtctgtagtccacatagtagacgccaggcatcttgaggttcttagcgggtttcttggatctatatgtggtcttgatgtttgcgatcagatggctcccgcccacgagcttcagggccatgtcgtttctgccttccaggccgccgtcagcggggtacagcgtctaggaactaagaaaggtcagacatcaggtgtatcatctgcggtccgtagaactccaagaatcgcccaaagaacctagatatacaccagaactacaaacgctagtctaccgagggcgggtgctcgaagtcccggtacagcaaagacggaaggtccggcggcagtcgccccatgtcgcagaBFP Exon 22,1402,1602,1802,2002,2202,2402,2602,2802,300
    cggtgaaggcctcccagccgagtgttttcttctgcatcacagggccgttggatgtgaagttcacccctctgatcttgacgttgtagatgaggcagccgtcctggaggctggtgtcctgggtagcggtcagcacgcccccgtcttcgtatgtggtgactctctcccatgtgaagccctcgccacttccggagggtcggctcacaaaagaagacgtagtgtcccggcaacctacacttcaagtggggagactagaactgcaacatctactccgtcggcaggacctccgaccacaggacccatcgccagtcgtgcgggggcagaagcatacaccactgagagagggtacacttcgggagBsu36IBFP Exon 22,3202,3402,3602,3802,4002,4202,4402,4602,480
    agggaaggactgcttgaagaagtcggggatgccctctgtgggacgacatgacttaaccagttaaccatgttctctgaacatgagaagggctggccactctccagagctcgaagttcctatactatttgaagaataggaacttcttgccttaacccagaaattatcactgttattcttttcccttcctgacgaacttcttcagcccctacgggagacaccctgctgtactgaattggtcaattggtacaagagacttgtactcttcccgaccggtgagaggtctcgagcttcaaggatatgataaacttcttatccttgaagaacggaattgggtctttaatagtgacaataagaaaHpaISacIHincIIEco53kICE donor/acceptorBFP Exon 2F3 Rev2,5002,5202,5402,5602,5802,6002,6202,6402,660
    agaatggtgcaaagagaagttcctataccttttgaagaataggaacttcgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcattcttaccacgtttctcttcaaggatatggaaaacttcttatccttgaagcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtaClaIBspDIBstBIEcoRIEcoRVWPREF5 Rev2,6802,7002,7202,7402,7602,7802,8002,8202,840
    gctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggacttcgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaBseRIBseRIPflMIWPRE2,8602,8802,9002,9202,9402,9602,9803,0003,020
    tcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacZraIAatIIWPRE3,0403,0603,0803,1003,1203,1403,1603,1803,200
    ctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgaccgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggAfeIBplIBplIClaIEarITliIXhoIBglIIBspDIPaeR7IWPREhGH polyA3,2203,2403,2603,2803,3003,3203,3403,3603,380
    cctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccAleICspCIhGH polyA3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattgccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataacBcgIBlpINsiISphIBbvCIBpu10IhGH polyA3,5803,6003,6203,6403,6603,6803,7003,720
    gccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctcggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagaEagINotIPmlIBsaXIBstXIRsrIIBstEIIhGH polyAR-ITR3,7403,7603,7803,8003,8203,8403,8603,8803,900
    gcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtcgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcaKasINarISbfISfoIPluTIPvuIIR-ITR3,9203,9403,9603,9804,0004,0204,0404,0604,080
    acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaf1 Origin4,1004,1204,1404,1604,1804,2004,2204,2404,260
    tagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaBsaXIf1 Origin4,2804,3004,3204,3404,3604,3804,4004,4204,440
    ttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcPsiI4,4604,4804,5004,5204,5404,5604,5804,6004,620
    cgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagZraIAatII4,6404,6604,6804,7004,7204,7404,7604,7804,800
    aggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtEarIAmpR4,8204,8404,8604,8804,9004,9204,9404,9604,980
    ttttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtgaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacacAmpR5,0005,0205,0405,0605,0805,1005,1205,1405,160
    gcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttcgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaaBcgIScaIAmpR5,1805,2005,2205,2405,2605,2805,3005,3205,340
    acttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggctgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgFspIPvuIAmpR5,3605,3805,4005,4205,4405,4605,4805,500
    gaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggaAseIAmpR5,5205,5405,5605,5805,6005,6205,6405,6605,680
    cccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttAmpR5,7005,7205,7405,7605,7805,8005,8205,8405,860
    gatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaactaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttpUC ori5,8805,9005,9205,9405,9605,9806,0006,0206,040
    gagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagAlwNIpUC ori6,0606,0806,1006,1206,1406,1606,1806,2006,220
    ttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggpUC ori6,2406,2606,2806,3006,3206,3406,3606,3806,400
    ggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaagpUC ori6,4206,4406,4606,4806,5006,5206,5406,5606,580
    ctggccttttgctggccttttgctcacatgtgaccggaaaacgaccggaaaacgagtgtacaPciIpUC ori6,600
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    6617 bp450050005500600050010001500L-ITR,SbfI,PvuII,EagI,NotI,MluI,MfeI,AgeI,EcoNIAflIIEF-1a,BfuAI,BspMI,BglII,PspOMI,ApaI,PciIFseI,PasI,BstAPI,EcoNI,Eco53kI,SacIBbvCIBpu10I,KasI,NarI,SfoI,PluTI,PaeR7I,TliI,XhoIlox2722,BamHIloxN,kozak,BstZ17I,SpeI,BsrGIBcgI,ScaIAmpR,PvuIFspIAseIpUC ori,AlwNI,PciI

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    6617