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  • 0 feature results
(pG4 = pKR509 ) pRS416-tRNA-HDV-S5-Ade2-Qi-gRNA + Tef1-Cas9 with RPL25 intron
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGAAACTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCCTGCCTAGCGAACGGACATTGAATGTGCGCGGAGCATAGAAAATTACTACCTTATTAAACCCTTAAATGAGACACAAATAAATAAAAATACAAAACATAAACCTAAAATCTTTCEN/ARS20406080100120140160
    GTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAATCACAGGATTTTCGTGTGTGCATTTATTTCTTCCATCTTCTCAATGCCTTACTTCTTTTTTTTTATTTGTTTCCAAATTTTTTAAAGTTGTTTTTCGCATGAAATGTATATATAAATAATCTGTTCTTTTCGTCTAATTTATCTATATGTAAGCTAATTGCTATTCATTTTACATTTTAGTGTCCTAAAAGCACACACCEN/ARS180200220240260280300320340
    GTCTTCTACACAGACAAGATGAAACAATTCGGCATTAATACCTGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATCAGAAGATGTGTCTGTTCTACTTTGTTAAGCCGTAATTATGGACTCTCGTCCTTCTCGTTCTATTTTCCATCATAAACAACCGCTAGGGGGATCTCAGAAAATGTAGAAGCCTTTTGTTTTTGATAAAAAAGAAATTAAAGAAAAAAATGAAAGATAAAAATTAAATATATAAATATACEN/ARS360380400420440460480500520
    TAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATGAAAAGGACCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAATTTTTTAAATTTAATATTAATAAAAATATCGTGCACTACTTTTCCTGGGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTPmlISwaIAmpR promoterCEN/ARS540560580600620640660680700
    GTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCAmpR open reading frame (ORF)720740760780800820840860880
    CCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTAmpR open reading frame (ORF)9009209409609801,0001,0201,0401,060
    GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTAmpR open reading frame (ORF)1,0801,1001,1201,1401,1601,1801,2001,2201,240
    CCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCACTNmeAIIIAmpR open reading frame (ORF)1,2601,2801,3001,3201,3401,3601,3801,4001,420
    GCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATAhdIAmpR open reading frame (ORF)AmpR terminator1,4401,4601,4801,5001,5201,5401,5601,5801,600
    CTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAAmpR terminatorori1,6201,6401,6601,6801,7001,7201,7401,7601,780
    TGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGori1,8001,8201,8401,8601,8801,9001,9201,940
    TCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCori1,9601,9802,0002,0202,0402,0602,0802,1002,120
    CTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCori2,1402,1602,1802,2002,2202,2402,2602,2802,300
    GGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCTTAATTAAGTATATGTGTTATGTAGTATACTCTTTCTTCAACAATTAAATACTCTCGGTAGCCAAGTTGGTTTAAGGCGCAAGACTGTAATTTATCACTACGACCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGAATTAATTCATATACACAATACATCATATGAGAAAGAAGTTGTTAATTTATGAGAGCCATCGGTTCAACCAAATTCCGCGTTCTGACATTAAATAGTGATGCTPacItRNA-Tyr (SUP4)US_gRNA_fwdori2,3202,3402,3602,3802,4002,4202,4402,4602,480
    AATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGAGATGGCCGGCATGGTCCCAGCCTCCTCGCTGGCGCCGGCTGGGCAACACCTTCGGGTGGCGAATGGGACTTTGGAGAAGTGACGCAAGCATCAAgtttaagagctatgctggaaacagcatagcaagtttaaataaggctaTTAGAACTCTAGCCCGCAAGCTGAGCGGGGGCCCTCTCTACCGGCCGTACCAGGGTCGGAGGAGCGACCGCGGCCGACCCGTTGTGGAAGCCCACCGCTTACCCTGAAACCTCTTCACTGCGTTCGTAGTTcaaattctcgatacgacctttgtcgtatcgttcaaatttattccgatAjuIHDV-RibozymeCas9 gRNA scaffoldtRNA-Tyr (SUP4)ADE2 gRNA 42,5002,5202,5402,5602,5802,6002,6202,6402,660
    gtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttGTTAACTCCGTTACTGTGGGCTTGCCATATTTTTTGGAACTTTTCTGCCCTTTTTCTCAATGAGTAAGGAGGGCGTTTATCTTTTCTATTTTATTTTTCTACAAATTTGATTCATGAGTCCATGGAACAAATAcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaCAATTGAGGCAATGACACCCGAACGGTATAAAAAACCTTGAAAAGACGGGAAAAAGAGTTACTCATTCCTCCCGCAAATAGAAAAGATAAAATAAAAAGATGTTTAAACTAAGTACTCAGGTACCTTGTTTATHpaINcoIU5 terminator (SNR7)Cas9 gRNA scaffold2,6802,7002,7202,7402,7602,7802,8002,8202,840
    TATAGAACTCATTTTCTTGTTTCGGTATATATATTAACTCCATTGAAGCATTTGCTTTTATCCTGACTGGCTAGCtagtcgtgcaatgtatgactttaagCCGCGGTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCATATCTTGAGTAAAAGAACAAAGCCATATATATAATTGAGGTAACTTCGTAAACGAAAATAGGACTGACCGATCGatcagcacgttacatactgaaattcGGCGCCAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGBmtINheISacIIU5 terminator (SNR7)DS_gRNA_rev2,8602,8802,9002,9202,9402,9602,9803,0003,020
    GCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTACGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAAT3,0403,0603,0803,1003,1203,1403,1603,1803,200
    GGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTcaCACAggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggAGCTCATAGCTTCAAAATGTTTCTACTCCTTTTTTCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAgtGTGTcctttgtcgatactggtactaatgcggttcgcgcgttaattgggagtgatttcccttgttttcgaccTCGAGTATCGAAGTTTTACAAAGATGAGGAAAAAASacIEco53kITEF PromoterT3_promoterM13_rev3,2203,2403,2603,2803,3003,3203,3403,3603,380
    ACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAATGAGAAGGTCTAAAAGAGCCTGAGGCGCGTAGCGGCATGGTGAAGTTTTGTGGGTTCGTGTCGTATGATTTAAAGGGGAGAAAGAAGGAGATCCCACAGCAATTAATGGGCATGATTTCCAAACCTTTTCTTTTTTCTCTGGCGGAGCAAAGAAAAAGAAGCAGCTTTTTCCGTTATTTEF Promoter3,4003,4203,4403,4603,4803,5003,5203,5403,560
    AAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCATTTAAAAATAGTGCAAAGAAAAAGAACTTTTAAAAAAAAAACTAAAAAAAGAGAAAGCTACTGGAGGGTAACTATAAATTCAATTATTTGCCAGAAGTTAAAGAGTTCAAAGTCAAAGTAAAAAGAACAAGATAATGTTGAAAAAAATGAAGAACGAGTAATCTTTCTTTCGTATCGTTEF Promoter3,5803,6003,6203,6403,6603,6803,7003,720
    ATCTAATCTAAGTTTTCTAGAGatgtctagagccccaaagaagaagagaaaagttagacccgggATGGACAAGAAGTACTCCATTGGGCTCGATATCGGCACAAACAGCGTCGGCTGGGCCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGTTCTGGGCAATACCTAGATTAGATTCAAAAGATCTCtacagatctcggggtttcttcttctcttttcaatctgggcccTACCTGTTCTTCATGAGGTAACCCGAGCTATAGCCGTGTTTGTCGCAGCCGACCCGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACCCGTTATGGXbaIXbaIEcoRVNmeAIIIMD2KK4YS6IG8LD10IG12TN14SV16GW18AV20IT22DE24YK26VP28SK30KF32KV34LG36NT383803-8356M1S2R3A4P5K6K7K8R9K10V11R12P13G143761-3802Cas9 exon 1nuclear localizati...(NLS) with linkerTEF Promoter3,7403,7603,7803,8003,8203,8403,8603,8803,900
    GATCGCCACAGCATAAAGAAGAACCTCATTGGCGCCCTCCTGTTCGACTCCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCGCAGAAAGAATCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAGGTGGATGACTCTTTCTCTAGCGGTGTCGTATTTCTTCTTGGAGTAACCGCGGGAGGACAAGCTGAGGCCCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTTTCTTAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGAGAAAGASbfIBglIIDR40HS42IK44KN46LI48GA50LL52FD54SG56ET58AE60AT62RL64KR66TA68RR70RY72TR74RK76NR78IC80YL82QE84IF86SN88EM90AK92VD94DS96F3803-8356Cas9 exon 13,9203,9403,9603,9804,0004,0204,0404,0604,080
    TCCATAGGCTGGAGGAGTCCTTTTTGGTGGAGGAGGATAAAAAGCACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTACCCAACCATATATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACTTGCGGTTGATCTATCTCGCAGGTATCCGACCTCCTCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGGTGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATATAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAGAGCGXcmIPflMIPflMIF98HR100LE102ES104FL106VE108ED110KK112HE114RH116PI118FG120NI122VD124EV126AY128HE130KY132PT134IY136HL138RK140KL142VD144ST146DK148AD150LR152LI154YL156A3803-8356Cas9 exon 14,1004,1204,1404,1604,1804,2004,2204,2404,260
    GCTGGCGCATATGATCAAATTTCGGGGACACTTCCTCATCGAGGGGGACCTGAACCCAGACAACAGCGATGTCGACAAACTCTTTATCCAACTGGTTCAGACTTACAATCAGCTTTTCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAAGCAATCCTGAGCGCTAGGCTGCGACCGCGTATACTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGGGTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGTCGAAAAGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCGACNdeISalIBspEIEcoNIL158AH160MI162KF164RG166HF168LI170EG172DL174NP176DN178SD180VD182KL184FI186QL188VQ190TY192NQ194LF196EE198NP200IN202AS204GV206DA208KA210IL212SA214RL2163803-8356Cas9 exon 14,2804,3004,3204,3404,3604,3804,4004,4204,440
    TCCAAATCCCGGCGGCTCGAAAACCTCATCGCACAGCTCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCTGACCCCCAACTTTAAATCTAACTTCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGCAAAGACACCTACGATGATGATCTCGAGGTTTAGGGCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGCCGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAATTTAGATTGAAGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGCPasIBsaBISK218SR220RL222EN224LI226AQ228LP230GE232KK234NG236LF238GN240LI242AL244SL246GL248TP250NF252KS254NF256DL258AE260DA262KL264QL266SK268DT270YD272DD274L3803-8356Cas9 exon 14,4604,4804,5004,5204,5404,5604,5804,6004,620
    ACAATCTGCTGGCCCAGATCGGCGACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAGTGATATTCTGCGAGTGAACACGGAGATCACCAAAGCTCCGCTGAGCGCTAGTATGATCAAGCGCTATGATGAGCACCACCAAGACTTGACTTTGCTTGTTAGACGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGGACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCCTCTAGTGGTTTCGAGGCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGABlpID276NL278LA280QI282GD284QY286AD288LF290LA292AK294NL296SD298AI300LL302SD304IL306RV308NT310EI312TK314AP316LS318AS320MI322KR324YD326EH328HQ330DL332TL334L3803-8356Cas9 exon 14,6404,6604,6804,7004,7204,7404,7604,7804,800
    GAAGGCCCTTGTCAGACAGCAACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGGATACATTGACGGCGGAGCAAGCCAGGAGGAATTTTACAAATTTATTAAGCCCATCTTGGAAAAAATGGACGGCACCGAGGAGCTGCTGGTAAAGCTTAACCTTCCGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGATTTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGTCCTCCTTAAAATGTTTAAATAATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGK336AL338VR340QQ342LP344EK346YK348EI350FF352DQ354SK356NG358YA360GY362ID364GG366AS368QE370EF372YK374FI376KP378IL380EK382MD384GT386EE388LL390VK392LN3943803-8356Cas9 exon 14,8204,8404,8604,8804,9004,9204,9404,9604,980
    AGAGAAGATCTGTTGCGCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACTGCACGCTATCCTCAGGCGGCAAGAGGATTTCTACCCCTTTTTGAAAGATAACAGGGAAAAGATTGAGAAAATCCTCACATTTCGGATACCCTACTATGTAGGCCTCTCTTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGGACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTCCTAAAGATGGGGAAAAACTTTCTATTGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCGGBglIIBsu36IRE396DL398LR400KQ402RT404FD406NG408SI410PH412QI414HL416GE418LH420AI422LR424RQ426ED428FY430PF432LK434DN436RE438KI440EK442IL444TF446RI448PY450YV452G3803-8356Cas9 exon 15,0005,0205,0405,0605,0805,1005,1205,1405,160
    CCCTCGCCCGGGGAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAACTTCGAGGAAGTCGTGGATAAGGGGGCCTCTGCCCAGTCCTTCATCGAAAGGATGACTAACTTTGATAAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGGGAGCGGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGACCTTGAAGCTCCTTCAGCACCTATTCCCCCGGAGACGGGTCAGGAAGTAGCTTTCCTACTGATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGABsaBIP454LA456RG458NS460RF462AW464MT466RK468SE470ET472IT474PW476NF478EE480VV482DK484GA486SA488QS490FI492ER494MT496NF498DK500NL502PN504EK506VL508PK510HS512L3803-8356Cas9 exon 15,1805,2005,2205,2405,2605,2805,3005,3205,340
    GCTGTACGAGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAAAGCCAGCATTCCTGTCTGGAGAGCAGAAGAAAGCTATCGTGGACCTCCTCTTCAAGACGAACCGGAAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATTCGACATGCTCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTTTCGGTCGTAAGGACAGACCTCTCGTCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCTTGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTAABarISacIEco53kIL514YE516YF518TV520YN522EL524TK526VK528YV530TE532GM534RK536PA538FL540SG542EQ544KK546AI548VD550LL552FK554TN556RK558VT560VK562QL564KE566DY568FK570KI5723803-8356Cas9 exon 15,3605,3805,4005,4205,4405,4605,4805,500
    GAATGTTTCGACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACGATCTCCTGAAAATCATTAAAGACAAGGACTTCCTGGACAATGAGGAGAACGAGGACATTCTTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGATGACTTACAAAGCTGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGCTAGAGGACTTTTAGTAATTTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAGAACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCTACTPasIPflFITth111IEC574FD576SV578EI580SG582VE584DR586FN588AS590LG592TY594HD596LL598KI600IK602DK604DF606LD608NE610EN612ED614IL616ED618IV620LT622LT624LF626ED628RE630M3803-8356Cas9 exon 15,5205,5405,5605,5805,6005,6205,6405,6605,680
    TTGAAGAACGCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCGCCGATATACAGGATGGGGGCGGCTGTCAAGAAAACTGATCAATGGGGTATGTGAACTGCAATATTAATAGCACGAGAAAATTGAGAGGAAGATAGATGGGAACTAGTAGAGAACTTCTTGCGAACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTATATGTCCTACCCCCGCCGACAGTTCTTTTGACTAGTTACCCCATACACTTGACGTTATAATTATCGTGCTCTTTTAACTCTCCTTCTATCTACCCTTGATCATCTCSpeIPflFITth111II632EE634RL636KT638YA640HL642FD644DK646VM648KQ650LK652RR654RY656TG658WG660RL662SR664KL666IN668GV670CE672LQ674Y*676*H678EK680IE682RK684ID686GN688**690S3803-8356Cas9 exon 1RPL25 Intron5,7005,7205,7405,7605,7805,8005,8205,8405,860
    TTGATATTGATGAGATACGAAAACCACACGTAAATAAACTATCCGACGACAAGAATAGTGCATTAAGGACTTGATTAAGATATGGTGAGCAACGTAATTATCGGGCTCAACAGTTTATTAGCAATCGTTTTGATAGAAGCGTTGATGCTGTGGAAGTTGTTCTTTTTACTACCAACAGAACTATAACTACTCTATGCTTTTGGTGTGCATTTATTTGATAGGCTGCTGTTCTTATCACGTAATTCCTGAACTAATTCTATACCACTCGTTGCATTAATAGCCCGAGTTGTCAAATAATCGTTAGCAAAACTATCTTCGCAACTACGACACCTTCAACAAGAAAAATGATGGTTGTC*692Y*694*D696TK698TT700RK702*T704IR706RQ708E*710CI712KD714LI716KI718W*720AT722*L724SG726ST728VY730*Q732SF734**736KR738*C740CG742SC744SF746YY748QQ7503803-8356RPL25 Intron5,8805,9005,9205,9405,9605,9806,0006,0206,040
    CCATTAACAAATCCATTAGAGTGTTCGTTCGTTTTTCTGCTCAGATTCAAGAAAATTATTCCATCCTCATTATACTTTTTTCTTCTATTTCGTGCTCCACGTCGAGGTATCAAGGAACATAGTTTACTAACATTAACGAATTCATCTCCTATGAATTTACTTTTTGTATAGATCCGAGGGTAATTGTTTAGGTAATCTCACAAGCAAGCAAAAAGACGAGTCTAAGTTCTTTTAATAAGGTAGGAGTAATATGAAAAAAGAAGATAAAGCACGAGGTGCAGCTCCATAGTTCCTTGTATCAAATGATTGTAATTGCTTAAGTAGAGGATACTTAAATGAAAAACATATCTAGGCTCPsrIBmgBICspCIEcoRIPL752TN754PL756EC758SF760VF762LL764RF766KK768II770PS772SL774YF776FL778LF780RA782PR784RG786IK788EH790SL792LT794LT796NS798SP800MN802LL804FV806*I808R3803-8356RPL25 Intron6,0606,0806,1006,1206,1406,1606,1806,2006,220
    ACAAGCAGAGTGGAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGCCAACCGGAACTTCATGCAGTTGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACAAGTTTCTGGCCAGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCTGTTCGTCTCACCTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACGGTTGGCCTTGAAGTACGTCAACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGGTCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGAjuIAflIID810KQ812SG814KT816IL818DF820LK822SD824GF826AN828RN830FM832QL834IH836DD838SL840TF842KE844DI846QK848AQ850VS852GQ854GD856SL858HE860HI862AN864LA866GS868P3803-8356Cas9 exon 26,2406,2606,2806,3006,3206,3406,3606,3806,400
    AGCTATCAAAAAGGGAATACTGCAGACCGTTAAGGTCGTGGATGAACTCGTCAAAGTAATGGGAAGGCATAAGCCCGAGAATATCGTTATCGAGATGGCCCGAGAGAACCAAACTACCCAGAAGGGACAGAAGAACAGTAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAAGAATCGATAGTTTTTCCCTTATGACGTCTGGCAATTCCAGCACCTACTTGAGCAGTTTCATTACCCTTCCGTATTCGGGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCTTGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTA870IK872KG874IL876QT878VK880VV882DE884LV886KV888MG890RH892KP894EN896IV898IE900MA902RE904NQ906TT908QK910GQ912KN914SR916ER918MK920RI922EE924GI926KE9283803-8356Cas9 exon 26,4206,4406,4606,4806,5006,5206,5406,5606,580
    CTGGGGTCCCAAATCCTTAAGGAACACCCAGTTGAAAACACCCAGCTTCAGAATGAGAAGCTCTACCTGTACTACCTGCAGAACGGCAGGGACATGTACGTGGATCAGGAACTGGACATCAATCGGCTCTCCGACTACGACGTGGATCATATCGTGCCCCAGTCTTTTCTCAAAGATGGACCCCAGGGTTTAGGAATTCCTTGTGGGTCAACTTTTGTGGGTCGAAGTCTTACTCTTCGAGATGGACATGATGGACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGATGCTGCACCTAGTATAGCACGGGGTCAGAAAAGAGTTTCTACKflIPciIAflIIBmgBILG930SQ932IL934KE936HP938VE940NT942QL944QN946EK948LY950LY952YL954QN956GR958DM960YV962DQ964EL966DI968NR970LS972DY974DV976DH978IV980PQ982SF984LK986D3803-8356Cas9 exon 26,6006,6206,6406,6606,6806,7006,7206,7406,760
    ATTCTATTGATAATAAAGTGTTGACAAGATCCGATAAAAATAGAGGGAAGAGTGATAACGTCCCCTCAGAAGAAGTTGTCAAGAAAATGAAAAATTATTGGCGGCAGCTGCTGAACGCCAAACTGATCACACAACGGAAGTTCGATAATCTGACTAAGGCTGAACGAGGTGGCCTGTCTAAGATAACTATTATTTCACAACTGTTCTAGGCTATTTTTATCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTCAACAGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCTTCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGD988SI990DN992KV994LT996RS998DK1000NR1002GK1004SD1006NV1008PS1010EE1012VV1014KK1016MK1018NY1020WR1022QL1024LN1026AK1028LI1030TQ1032RK1034FD1036NL1038TK1040AE1042RG1044GL1046S3803-8356Cas9 exon 26,7806,8006,8206,8406,8606,8806,9006,9206,940
    TGAGTTGGATAAAGCCGGCTTCATCAAAAGGCAGCTTGTTGAGACACGCCAGATCACCAAGCACGTGGCCCAAATTCTCGATTCACGCATGAACACCAAGTACGATGAAAATGACAAACTGATTCGAGAGGTGAAAGTTATTACTCTGAAGTCTAAGCTGGTCTCAGATTTCAGAAAGACTCAACCTATTTCGGCCGAAGTAGTTTTCCGTCGAACAACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGTTTAAGAGCTAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAATAATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCPmlIPsrIBstXIE1048LD1050KA1052GF1054IK1056RQ1058LV1060ET1062RQ1064IT1066KH1068VA1070QI1072LD1074SR1076MN1078TK1080YD1082EN1084DK1086LI1088RE1090VK1092VI1094TL1096KS1098KL1100VS1102DF1104RK11063803-8356Cas9 exon 26,9606,9807,0007,0207,0407,0607,0807,1007,120
    GACTTTCAGTTTTATAAGGTGAGAGAGATCAACAATTACCACCATGCGCATGATGCCTACCTGAATGCAGTGGTAGGCACTGCACTTATCAAAAAATATCCCAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGTACGATGTTAGGAAAATGATCGCAAAGTCTGAGCAGGCTGAAAGTCAAAATATTCCACTCTCTCTAGTTGTTAATGGTGGTACGCGTACTACGGATGGACTTACGTCACCATCCGTGACGTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACATGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCFspAIDF1108QF1110YK1112VR1114EI1116NN1118YH1120HA1122HD1124AY1126LN1128AV1130VG1132TA1134LI1136KK1138YP1140KL1142ES1144EF1146VY1148GD1150YK1152VY1154DV1156RK1158MI1160AK1162SE1164Q3803-8356Cas9 exon 27,1407,1607,1807,2007,2207,2407,2607,280
    AAATAGGCAAGGCCACCGCTAAGTACTTCTTTTACAGCAATATTATGAATTTTTTCAAGACCGAGATTACACTGGCCAATGGAGAGATTCGGAAGCGACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACAAGGGTAGGGATTTCGCGACAGTCCGGAAGGTCCTTTTATCCGTTCCGGTGGCGATTCATGAAGAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCTAATGTGACCGGTTACCTCTCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGTTCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGANruIBspEIE1166IG1168KA1170TA1172KY1174FF1176YS1178NI1180MN1182FF1184KT1186EI1188TL1190AN1192GE1194IR1196KR1198PL1200IE1202TN1204GE1206TG1208EI1210VW1212DK1214GR1216DF1218AT1220VR1222KV1224L3803-8356Cas9 exon 27,3007,3207,3407,3607,3807,4007,4207,4407,460
    GTCCATGCCGCAGGTGAACATCGTTAAAAAGACCGAAGTACAGACCGGAGGCTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAACAGCGACAAGCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTCGATTCTCCTACAGTCGCTTACAGTGTACTGGTTGTGCAGGTACGGCGTCCACTTGTAGCAATTTTTCTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGTCGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAAGAGGATGTCAGCGAATGTCACATGACCAACACKflIPaqCIS1226MP1228QV1230NI1232VK1234KT1236EV1238QT1240GG1242FS1244KE1246SI1248LP1250KR1252NS1254DK1256LI1258AR1260KK1262DW1264DP1266KK1268YG1270GF1272DS1274PT1276VA1278YS1280VL1282VV12843803-8356Cas9 exon 27,4807,5007,5207,5407,5607,5807,6007,6207,640
    GCCAAAGTGGAGAAAGGGAAGTCTAAAAAACTCAAAAGCGTCAAGGAACTGCTGGGCATCACAATCATGGAGCGATCAAGCTTCGAAAAAAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAAGACCTCATCATTAAGCTTCCCAAGTACTCTCTCTTTGAGCCGGTTTCACCTCTTTCCCTTCAGATTTTTTGAGTTTTCGCAGTTCCTTGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTTTTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGTAATTCGAAGGGTTCATGAGAGAGAAACTCGTliIXhoIPaeR7IAK1286VE1288KG1290KS1292KK1294LK1296SV1298KE1300LL1302GI1304TI1306ME1308RS1310SF1312EK1314NP1316ID1318FL1320EA1322KG1324YK1326EV1328KK1330DL1332II1334KL1336PK1338YS1340LF1342E3803-8356Cas9 exon 27,6607,6807,7007,7207,7407,7607,7807,8007,820
    TTGAAAACGGCCGGAAACGAATGCTCGCTAGTGCGGGCGAGCTGCAGAAAGGTAACGAGCTGGCACTGCCCTCTAAATACGTTAATTTCTTGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAAGATAATGAGCAGAAGCAGCTGTTCGTGGAACAACACAAACACTAAACTTTTGCCGGCCTTTGCTTACGAGCGATCACGCCCGCTCGACGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGAACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCGTCGACAAGCACCTTGTTGTGTTTGTGATL1344EN1346GR1348KR1350ML1352AS1354AG1356EL1358QK1360GN1362EL1364AL1366PS1368KY1370VN1372FL1374YL1376AS1378HY1380EK1382LK1384GS1386PE1388DN1390EQ1392KQ1394LF1396VE1398QH1400KH1402Y3803-8356Cas9 exon 27,8407,8607,8807,9007,9207,9407,9607,9808,000
    CCTTGATGAGATCATCGAGCAAATAAGCGAATTCTCCAAAAGAGTGATCCTCGCCGACGCTAACCTCGATAAGGTGCTTTCTGCTTACAATAAGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAACATTATCCACTTGTTTACTCTGACCAACTTGGGCGCGCCTGCAGCCTTCGGAACTACTCTAGTAGCTCGTTTATTCGCTTAAGAGGTTTTCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTATTCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACTGGTTGAACCCGCGCGGACGTCGGAAGAscIEcoNIEcoRIL1404DE1406II1408EQ1410IS1412EF1414SK1416RV1418IL1420AD1422AN1424LD1426KV1428LS1430AY1432NK1434HR1436DK1438PI1440RE1442QA1444EN1446II1448HL1450FT1452LT1454NL1456GA1458PA1460AF14623803-8356Cas9 exon 28,0208,0408,0608,0808,1008,1208,1408,1608,180
    AAGTACTTCGACACCACCATAGACAGAAAGCGGTACACCTCTACAAAGGAGGTCCTGGACGCCACACTGATTCATCAGTCAATTACGGGGCTCTATGAAACAAGAATCGACCTCTCTCAGCTCGGTGGAGACAGCAGGGCTGACCCCAAGAAGAAGAGGAAGGTGTGATCTCTTCTCGTTCATGAAGCTGTGGTGGTATCTGTCTTTCGCCATGTGGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGATACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGGGGTTCTTCTTCTCCTTCCACACTAGAGAAGAGCTliIXhoIPaeR7IKY1464FD1466TT1468ID1470RK1472RY1474TS1476TK1478EV1480LD1482AT1484LI1486HQ1488SI1490TG1492LY1494ET1496RI1498DL1500SQ1502LG1504GD1506SR1508AD1510PK1512KK1514RK1516V*15183803-8356Cas9 exon 28,2008,2208,2408,2608,2808,3008,3208,3408,360
    AGTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCTCAGTACATTAATCAATACAGTGCGAATGTAAGTGCGGGAGGGGGGTGTAGGCGAGATTGGCTTTTCCTTCCTCAATCTGTTGGACTTCAGATCCAGGGATAAATAAAAAAATATCAATACAATCATAATTCTTGCAATAAATATAAAGTTTAAAAAGAAAAAAAAGACATGTCTGCGMluIBsrGICYC1 Terminator8,3808,4008,4208,4408,4608,4808,5008,5208,540
    GTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCGGCCGGTACccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCACATGCGTACATTGTAATATGACTTTTGGAACGAACTCTTCCAAAACCCTGCGAGCTTCCGAAATTAAACGCCGGCCATGggttaagcgggatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgtTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCKpnIAcc65ICYC1 TerminatorlacZαT7T7_promoterM13-fwd8,5608,5808,6008,6208,6408,6608,6808,7008,720
    CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGlacZαf1 ori8,7408,7608,7808,8008,8208,8408,8608,8808,900
    CTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGAGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTf1 orilacZα8,9208,9408,9608,9809,0009,0209,0409,060
    CGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAAGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTAloIf1 ori9,0809,1009,1209,1409,1609,1809,2009,2209,240
    CAAAATATTAACGTTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAGGGTAATAACTGATATAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTTTAGTTTTGCTGGCCGCATCTTCTCGTTTTATAATTGCAAATGTTAAAGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATCCCATTATTGACTATATTAATTTAACTTCGAGATTAAACACTCAAATCATATGTACGTAAATGAATATTATGTCAAAAAATCAAAACGACCGGCGTAGAAGAGNsiI*268NQ266QG264CR262R9410-10213URA3 terminatorURA3f1 ori9,2609,2809,3009,3209,3409,3609,3809,4009,420
    AAATATGCTTCCCAGCCTGCTTTTCTGTAACGTTCACCCTCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAATAATAATGTCAGATCCTGTAGAGACCACATCATCCACGGTTCTATACTGTTGACCCAATGCGTCTCCCTTGTCATCTAAACCCACACCGGGTGTTTATACGAAGGGTCGGACGAAAAGACATTGCAAGTGGGAGATGGAATCGTAGGGAAGGGAAACGTTTATCAGGAGAAGGTTGTTATTATTACAGTCTAGGACATCTCTGGTGTAGTAGGTGCCAAGATATGACAACTGGGTTACGCAGAGGGAACAGTAGATTTGGGTGTGGCCCACL260YA258EW256GA254KR252YR250EG248EV246KA244DR242GK240AF238LG236RG234VI232II230DS228GT226SV224VD222DV220TR218YQ216QG214LA212DG210KD208DL206GV204GP202T9410-10213URA39,4409,4609,4809,5009,5209,5409,5609,5809,600
    TCATAATCAACCAATCGTAACCTTCATCTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAATCTTTGTCGCTCTTCGCAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATGACAATTCTGCTAACATCAAAAGGCCTCTAGGTTCCTTAGTATTAGTTGGTTAGCATTGGAAGTAGAGAAGGTGGGTACAGAGAAACTCGTTATTTCGGCTATTGTTTTAGAAACAGCGAGAAGCGTTACAGTTGTCATGGGAATCATATAAGAGGTCATCTATCCCTCGGGAACGTACTGTTAAGACGATTGTAGTTTTCCGGAGATCCAAGGAAStuIM200IL198WD196YG194ED192RG190GM188DR186QA184IF182GI180VF178DK176DS174KA172ID170VT168GK166TY164EG162TS160LS158GK156CS154LE152AL150ML148LG146RP144EK1429410-10213URA39,6209,6409,6609,6809,7009,7209,7409,7609,780
    TGTTACTTCTTCTGCCGCCTGCTTCAAACCGCTAACAATACCTGGGCCCACCACACCGTGTGCATTCGTAATGTCTGCCCATTCTGCTATTCTGTATACACCCGCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTCTGTCTTCGAAGAGTAAAAAATTGTACTTGACAATGAAGAAGACGGCGGACGAAGTTTGGCGATTGTTATGGACCCGGGTGGTGTGGCACACGTAAGCATTACAGACGGGTAAGACGATAAGACATATGTGGGCGTCTCATGACGTTAAACTGACATAATGGTTACAGTCGTTTAAAAGACAGAAGCTTCTCATTTTTTAACATGAACApaIPspOMITV140EE138AA136QK134LG132SV130IG128PG126VV124GH122AN120TI118DA116WE114AI112RY110VG108AS106YQ104LK102VT100NG98ID96AF94KR92DE90FL88LF86NY84K9410-10213URA39,8009,8209,8409,8609,8809,9009,9209,9409,960
    GCGGATAATGCCTTTAGCGGCTTAACTGTGCCCTCCATGGAAAAATCAGTCAAGATATCCACATGTGTTTTTAGTAAACAAATTTTGGGACCTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACATCCAATGAAGCACACAAGTTTGTTTGCTTTTCGTGCATGATATCGCCTATTACGGAAATCGCCGAATTGACACGGGAGGTACCTTTTTAGTCAGTTCTATAGGTGTACACAAAAATCATTTGTTTAAAACCCTGGATTACGAAGTTGATTGAGGTCATTAAGGAACCACCATGCTTGTAGGTTACTTCGTGTGTTCAAACAAACGAAAAGCACGTACTATANcoIPciIXcmIEcoRVA82SL80AK78LP76KV74TG72EM70SF68DT66LI64DV62HT60KL58LC56IK54PG52LA50EV48LE46LL44EK42TT40RV38DL36SA34CL32NT30QK28EH26MI24N9410-10213URA39,98010,00010,02010,04010,06010,08010,10010,12010,140
    TAAATAGCTTGGCAGCAACAGGACTAGGATGAGTAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTCGTTTCCTGCAGGTTTTTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGTTTCTTCAACACTACATATGCGTATATATACCAATCTAAGTCTATTTATCGAACCGTCGTTGTCCTGATCCTACTCATCGTCGTGCAAGGAATATACATCGAAAGCTGTACTAAATAGAAGCAAAGGACGTCCAAAAACAAGACACGTCAACCCAATTCTTATGACCCGTTAAAGTACAAAGAAGTTGTGATGTATACGCATATATATGGTTAGATTCAGANdeISbfIF22LK20AA18VP16SP14HT12AA10RE8KY6TA4KS2M9410-10213URA3 promoterURA310,16010,18010,20010,22010,24010,26010,28010,30010,320
    GTGCTCCTTCCTTCGTTCTTCCTTCTGTTCGGAGATTACCGAATCAAAAAAATTTCAAAGAAACCGAAATCAAAAAAAAGAATAAAAAAAAAATGATGAATTGAATTGAAAAGCTGTGGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCACGAGGAAGGAAGCAAGAAGGAAGACAAGCCTCTAATGGCTTAGTTTTTTTAAAGTTTCTTTGGCTTTAGTTTTTTTTCTTATTTTTTTTTTACTACTTAACTTAACTTTTCGACACCATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGGGCTGTGURA3 promoter10,34010,36010,38010,40010,42010,44010,46010,48010,500
    CCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCT10,52010,54010,56010,58010,60010,620
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10629 bp75008000850090009500100005001000150020002500US_gRNA_fwdf1 ori3803-83569410-10213CEN/ARS,SwaI,PmlIAmpR promoterAmpR open reading frame (ORF)NmeAIII,AhdIAmpR terminatororitRNA-Tyr (SUP4),PacIHDV-Ribozyme,ADE2 gRNA 4,AjuIEcoRI,EcoNIAscICYC1 Terminator,PaeR7I,TliI,XhoIT7,T7_promoter,M13-fwd,BsrGI,MluI,Acc65I,KpnIlacZαAloIURA3 terminator,NsiIURA3,StuI,PspOMI,ApaI,NcoI,EcoRVURA3 promoter,PciI,XcmI,SbfI,NdeI

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    10629