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pAAV-nEF-CoffFon-hChR2(E123T-T159C)-EYFP-WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaagctttgcaaagatggataggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcgaaacgtttctacctatMluISbfIAflIIIL-ITR20406080100120140160
    aagttttaaacagagaggaatctttgcagctaatggaccttctaggtcttgaaaggagtgggaattAAGGATCTGCGATCGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACGGGTGCCTAGAGAAGGttcaaaatttgtctctccttagaaacgtcgattacctggaagatccagaactttcctcacccttaaTTCCTAGACGCTAGCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGCCCACGGATCTCTTCCMfeIPvuIAsiSIEcoNInEF promoter180200220240260280300320340
    TGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGCTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCGACTTCGAAGCTCCCCGAGCGTAGAGAGGAAGTGCGCGGGnEF promoter360380400420440460480500520
    GCCGCCCTACCTGAGGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCCTCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACCGGGCCTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTCGGCGGGATGGACTCCGGCGGTAGGTGCGGCCAACTCAGCGCAAGACGGCGGAGGGCGGACACCACGGAGGACTTGACGCAGGCGGCAGATCCATTCAAATTTCGAGTCCAGCTCTGGCCCGGAAACAGGCCGCGAGGGAACCTCGGATGGATCTGAGTCGGCCGAGAGGTGCGACspCIEcoNIBsu36InEF promoter540560580600620640660680700
    TTGCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTTTCTGTTCTGCGCCGTTACAGATCCAAGCTGTGACCGGCGCCTACggatccgaagttcctattcttcaaatagtataggaacttcgcagagtagtagctggattgtagctgctattagcaatctaaaacctctAACGGACTGGGACGAACGAGTTGAGATGCAGAAACAAAGCAAAAGACAAGACGCGGCAATGTCTAGGTTCGACACTGGCCGCGGATGcctaggcttcaaggataagaagtttatcatatccttgaagcgtctcatcatcgacctaacatcgacgataatcgttagattttggagaKasINarISfoIBamHIPluTInEF promoterF3720740760780800820840860
    tagaagttcctattcttcaaaaggtataggaacttcggcgcgccttacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactggtagctcaggtagtggttgtcggatcttcaaggataagaagttttccatatccttgaagccgcgcggaatgaacatgtcgagcaggtacggctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagagcaaccccagaaacgagtcccgcctgaccatcgagtccatcaccaacagccAscIBsrGIEYFP Exon 3F58809009209409609801,0001,0201,040
    gcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccgtcgtcgtgccccggcagcggctacccccacaagacgaccatcaccagccgctcgacgtgcgacggcaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacagccggtactatatctgcaacaccgacaacatcaacatgaggtcgaacacgggEYFP Exon 31,0601,0801,1001,1201,1401,1601,1801,2001,220
    caggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcgtcctacaacggcaggaggaacttcagctacgggaagtcgagctacgccaagtggtcccacagcgggagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggacctgcatcggaagcccgtaccgcctgaacttcttcagcacgacgEYFP Exon 31,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttcatgtggtcggggtagcgggcgaagcactgcaggccgtagccgaaggtggtcacgagggtgggccagggcacgggcagctgtgggacgacatgacttaaccagttaaccatgttctctgaacatgagaagggctggccactctccagtcgacataacttcgtataggatacttaagtacaccagccccatcgcccgcttcgtgacgtccggcatcggcttccaccagtgctcccacccggtcccgtgcccgtcgacaccctgctgtactgaattggtcaattggtacaagagacttgtactcttcccgaccggtgagaggtcagctgtattgaagcatatcctatgaaHpaISalIHincIIHincIIEYFP Exon 3CE intron BPlox27221,4201,4401,4601,4801,5001,5201,5401,560
    tatacgaagttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagtataccttatacgaagttatacTAGTcctcctgtactcaccttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgatatgcttcaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattattgaagcatatcatatggaatatgcttcaatatgATCAggaggacatgagtggaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgggagcSpeIBstZ17IEYFP Exon 2loxNCE i...norlox27221,5801,6001,6201,6401,6601,6801,7001,7201,740
    ccctcgccggacacgctgaacttgtggccgtttacgtcaccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcacCATGGTggcggccgctggcacggctccggcctcggcttcgtcttcgacgagagtctcgacctcgatctccgttccgcgggagcggcctgtgcgacttgaacaccggcaaatgcagtggcaggtcgagctggtcctacccgtggtggggccacttgtcgaggagcgggaacgagtgGTACCAccgccggcgaccgtgccgaggccggagccgaagcagaagctgctctcagagctggagctagaggcaaggcgPshAIEYFP Exon 2ChR2 ET/TC Exon 21,7601,7801,8001,8201,8401,8601,8801,9001,920
    cgatgttcagtttggtggttttgcggatatctccgtgaatcaatatgtgctcgtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttactcatcagatcaataatcgtgtgacctacggtggagccatagacgctcaggacgccaaaaccttcgggccccaagctacaagtcaaaccaccaaaacgcctatagaggcacttagttatacacgagcacctagtcctgcgcgtccatcacagggttgttgggggttgttaaaaatgagtagtctagttattagcacactggatgccacctcggtatctgcgagtcctgcggttttggaagcccggggttApaIBfuAIBspMIEcoRVPspOMIChR2 ET/TC Exon 21,9401,9601,9802,0002,0202,0402,0602,0802,100
    aatgaagagaattgggaacataccccagctcacgaaaaacagccatgccatgccggtcacgacctggcggcaccgaccctttggcacagtatgataaccctcgatatatgctttggcggcgtgaaaaaatgtgttcgcgccatagcacaatccaagacaaaagaagatgactttattacttctcttaacccttgtatggggtcgagtgctttttgtcggtacggtacggccagtgctggaccgccgtggctgggaaaccgtgtcatactattgggagctatatacgaaaccgccgcacttttttacacaagcgcggtatcgtgttaggttctgttttcttctactgaaatChR2 ET/TC Exon 22,1202,1402,1602,1802,2002,2202,2402,260
    acatagccggttgccatggcgctggtagccccccacacgatacacccgatgtctgagacaaggagtcccatggttctcctgctgtagtcgttgctcaggcctaaacacatgagaaatagagtcataagcacatgggtcacatacaggagatatgtatataacattaatacaattttgtatcggccaacggtaccgcgaccatcggggggtgtgctatgtgggctacagactctgttcctcagggtaccaagaggacgacatcagcaacgagtccggatttgtgtactctttatctcagtattcgtgtacccagtgtatgtcctctatacatatattgtaattatgttaaaAseIStuIChR2 ET/TC Exon 2CMV intron branch pointCMV i...ptor2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tataaaaaaaagggggggcacaGGTACcataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataaggtatactatacgaagttatgctagcaaccccgacacttaccggtgaggatattttttttcccccccgtgtCCATGgtattgaagcatatttcataggatatgcttcaataaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattccatatgatatgcttcaatacgatcgttggggctgtgaatggccactccAgeIBmtIKpnINheIPsiIXmnIAcc65IBstZ17Ilox2722 RevloxN RevCMV intr...h pointCMV ...nor2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ttgctcaggtggataaggatgacaggacaagtgagcagccaggttgcatagcgcagccactgcacccggtgtcctgtggcaaggtagagcatagagggattcttaaactcaaaaaagaactcgagaatcaccttaaccatttcaatggcgcacacatagatctcctcccagccgcaacgagtccacctattcctactgtcctgttcactcgtcggtccaacgtatcgcgtcggtgacgtgggccacaggacaccgttccatctcgtatctccctaagaatttgagttttttcttgagctcttagtggaattggtaaagttaccgcgtgtgtatctagaggagggtcggcgTliIXhoIAlwNIBglIIBstAPIDraIIIPaeR7IChR2 ET/TC Exon 12,6402,6602,6802,7002,7202,7402,7602,7802,800
    atgtagatttccaggtttggtaggcatagaacatcagcagcaaaatgctgaatcctgctgcaagccactgcaggacatttgacgcggtctgagcgccgttcgtgccgcgagattcaatccatccggcacagtaacattgatcctcagggaccaggacggacccgttcaccaccactacatctaaaggtccaaaccatccgtatcttgtagtcgtcgttttacgacttaggacgacgttcggtgacgtcctgtaaactgcgccagactcgcggcaagcacggcgctctaagttaggtaggccgtgtcattgtaactaggagtccctggtcctgcctgggcaagtggtggtgPflFIBsu36ITth111IChR2 ET/TC Exon 12,8202,8402,8602,8802,9002,9202,9402,960
    aggattagtaacgaacaaaagttcgcgtccgacggcagacaaagcgccgccatagtccatggtggcgagctcgaagttcctatactatttgaagaataggaacttcttgccttaacccagaaattatcactgttattctttagaatggtgcaaagagaagttcctataccttttgtcctaatcattgcttgttttcaagcgcaggctgccgtctgtttcgcggcggtatcaggtaccaccgctcgagcttcaaggatatgataaacttcttatccttgaagaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttctcttcaaggatatggaaaacSacIEco53kIChR2 ET/TC Exon 1F3 RevF5 Rev2,9803,0003,0203,0403,0603,0803,1003,1203,140
    aagaataggaacttcgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctttcttatccttgaagcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaClaIBspDIBstBIEcoRIEcoRVWPREF5 Rev3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgPflMIWPRE3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccaccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtZraIAatIIWPRE3,5203,5403,5603,5803,6003,6203,6403,660
    gcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgAfeIBplIBplIClaITliIXhoIBglIIBspDISacIIPaeR7IWPREhGH polyA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagcggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgCspCIhGH polyA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggtBlpINsiISphIBbvCIhGH polyA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    actcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgtgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcPmlIBsaAIRsrIIBstEIIhGH polyAR-ITR4,2204,2404,2604,2804,3004,3204,3404,360
    ctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctggagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacKasINarISbfISfoIPluTIR-ITR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaf1 Origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaataPsiIBsaAIDraIIIf1 Origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    aagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcttccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcg4,9204,9404,9604,9805,0005,0205,0405,060
    gccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcZraI5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacAatIIAmpR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagaXmnIAmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactcgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgaScaIAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    gcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtFspIPvuIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactgggttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgacccAseIAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaacggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcpUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    cggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgAlwNIpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaapUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori7,0207,0407,060
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    7063 bp500055006000650050010001500EYFP Exon 2

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    7063