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pC0049 EF1a-dCas13b-NES-HIV, H133A/H1058A
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaacagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttSgrDI20406080100120140160
    gaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgacttagacgaatcccaatccgcaaaacgcgacgaagcgctacatgcccggtctatatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactMluINruISpeICMV enhancer180200220240260280300320340
    ccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatNdeICMV enhancer360380400420440460480500520
    aatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcaCspCISnaBICMV enhancer540560580600620640660680700
    caatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctcgttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtcgcgcaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagag5'LTR720740760780800820840860
    tggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctKasINarISfoIPluTI5'LTR8809009209409609801,0001,0201,040
    aaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaattggtctcctcgagagagctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttNruI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagattaagccaattccggtccccctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatct1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtaatatattatgtcatcgttgggagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggcgactagaagtctggacctcctcctctatactccctgttaacctctEagINotISapIBspQI1,4201,4401,4601,4801,5001,5201,5401,560
    agtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtcacttaatatatttatatttcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccRRE1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggatgtccggtctgttaataacagaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccBbvCIRRE1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaacgagaccttttgagtaaacgtggtgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcAleIBsmI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcacttacttgttcttaataaccttaatctatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtg2,1202,1402,1602,1802,2002,2202,2402,260
    cattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaattttaaaagaagtaatagcaaagtctgggtggagggttggggctcccctgggctgtccgggcttccttatcttcttcttccacctctctctctgtctctgtctaggtaagctaatcacttgcctagccgtgacgcacgcggttaagacgtctgtttaccgtcataagtaggtgttaaaattttcttKflI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttggttaattaatgcaaagatggataaagttttaaattcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtccctgtcgtctctaggtcaaaccaattaattacgtttctacctatttcaaaatttPacIEF-1a2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cagagaggaatctttgcagctaatggaccttctaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaaggtctctccttagaaacgtcgattacctggaagatccagaactttcctcacccttaaccgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttggccacggatctcttccaccgcgccccatttgaccctttcEF-1a2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgcctactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtccattcacggcacacaccaagggcgcccggaccggagaaatgcccaataccgggaacgcacggaEF-1a2,8202,8402,8602,8802,9002,9202,9402,960
    tgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgacttaatgaaggtgaccgacgtcatgcactaagaactagggctcgaagcccaaccttcacccaccctctcaagctccggaacgcgaattcctcggggaagcggagcacgaactcaactccggaccggacccgcgaccccggcggcgcacgcttagaccaccgtggaagcgcggacEF-1a2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgagagcgacgaaagctattcagagatcggtaaattttaaaaactactggacgacgctgcgaaaaaaagaccgttctatcagaacatttacgcccggttctagacgtgtgaccataaagccaaaaaccccggcgcccgccgctgccccgggcacgcagggtcgcgtgtacaagccgcApaIPspOMIEF-1a3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgtccgccccggacgctcgcgccggtggctcttagcctgcccccatcagagttcgaccggccggacgagaccacggaccggagcgcggcggcacatagcggggcgggacccgccgttccgaccgggccagccgtggtcaacgcactcgcctttctaccggcgaagggccgggacgacFseIPasIEF-1a3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttagggtccctcgagttttacctcctgcgccgcgagccctctcgcccgcccactcagtgggtgtgtttccttttcccggaaaggcaggagtcggcagcgaagtacactgaggtgcctcatggcccgcggcaggtccgtggagctaatcaagagctcgaaaacctcatgcagcagaaatccKasINarISfoITliIXhoIBbvCIPluTIPaeR7IEF-1a3,5203,5403,5603,5803,6003,6203,6403,660
    ttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcagaacccccctccccaaaatacgctacctcaaaggggtgtgactcacccacctctgacttcaatccggtcgaaccgtgaactacattaagaggaaccttaaacgggaaaaactcaaacctagaaccaagtaagagttcggagtctgtcaccaagtttcaaaaaaagaaggtaaagtcEF-1a3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtgtcgtgacGTACGGCCACCATGaacatccccgctctggtggaaaaccagaagaagtactttggcacctacagcgtgatggccatgctgaacgctcagaccgtgctggaccacatccagaaggtggccgatattgagggcgagcagaacgagaacaacgagaatctgtggtttccacagcactgCATGCCGGTGGTACttgtaggggcgagaccaccttttggtcttcttcatgaaaccgtggatgtcgcactaccggtacgacttgcgagtctggcacgacctggtgtaggtcttccaccggctataactcccgctcgtcttgctcttgttgctcttagacaccaaagScaIBsiWIPflMIMN2IP4AL6VE8NQ10KK12YF14GT16YS18VM20AM22LN24AQ26TV28LD30HI32QK34VA36DI38EG40EQ42NE44NN46EN48LW50F3872-7273dPspCas13b H133A/H1058A3,8603,8803,9003,9203,9403,9603,9804,0004,020
    accccgtgatgagccacctgtacaacgccaagaacggctacgacaagcagcccgagaaaaccatgttcatcatcgagcggctgcagagctacttcccattcctgaagatcatggccgagaaccagagagagtacagcaacggcaagtacaagcagaaccgcgtggaagtgaacagtggggcactactcggtggacatgttgcggttcttgccgatgctgttcgtcgggctcttttggtacaagtagtagctcgccgacgtctcgatgaagggtaaggacttctagtaccggctcttggtctctctcatgtcgttgccgttcatgttcgtcttggcgcaccttcacttgtcH52PV54MS56HL58YN60AK62NG64YD66KQ68PE70KT72MF74II76ER78LQ80SY82FP84FL86KI88MA90EN92QR94EY96SN98GK100YK102QN104RV106EV108NS1103872-7273dPspCas13b H133A/H1058A4,0404,0604,0804,1004,1204,1404,1604,1804,200
    caacgacatcttcgaggtgctgaagcgcgccttcggcgtgctgaagatgtacagggacctgaccaacGCAtacaagacctacgaggaaaagctgaacgacggctgcgagttcctgaccagcacagagcaacctctgagcggcatgatcaacaactactacacagtggccctgcgggttgctgtagaagctccacgacttcgcgcggaagccgcacgacttctacatgtccctggactggttgCGTatgttctggatgctccttttcgacttgctgccgacgctcaaggactggtcgtgtctcgttggagactcgccgtactagttgttgatgatgtgtcaccgggacgccND112IF114EV116LK118RA120FG122VL124KM126YR128DL130TN132AY134KT136YE138EK140LN142DG144CE146FL148TS150TE152QP154LS156GM158IN160NY162YT164VA166LR1683872-7273dPspCas13b H133A/H1058AHEPN14,2204,2404,2604,2804,3004,3204,3404,360
    aacatgaacgagagatacggctacaagacagaggacctggccttcatccaggacaagcggttcaagttcgtgaaggacgcctacggcaagaaaaagtcccaagtgaataccggattcttcctgagcctgcaggactacaacggcgacacacagaagaagctgcacctgagcggagttgtacttgctctctatgccgatgttctgtctcctggaccggaagtaggtcctgttcgccaagttcaagcacttcctgcggatgccgttctttttcagggttcacttatggcctaagaaggactcggacgtcctgatgttgccgctgtgtgtcttcttcgacgtggactcgcctcAloIBarISbfINM170NE172RY174GY176KT178ED180LA182FI184QD186KR188FK190FV192KD194AY196GK198KK200SQ202VN204TG206FF208LS210LQ212DY214NG216DT218QK220KL222HL224SG2263872-7273dPspCas13b H133A/H1058A4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tgggaatcgccctgctgatctgcctgttcctggacaagcagtacatcaacatctttctgagcaggctgcccatcttctccagctacaatgcccagagcgaggaacggcggatcatcatcagatccttcggcatcaacagcatcaagctgcccaaggaccggatAcacagcgagaaacccttagcgggacgactagacggacaaggacctgttcgtcatgtagttgtagaaagactcgtccgacgggtagaagaggtcgatgttacgggtctcgctccttgccgcctagtagtagtctaggaagccgtagttgtcgtagttcgacgggttcctggcctaTgtgtcgctcttBsaBIVG228IA230LL232IC234LF236LD238KQ240YI242NI244FL246SR248LP250IF252SS254YN256AQ258SE260ER262RI264II266RS268FG270IN272SI274KL276PK278DR280IH282SE284K3872-7273dPspCas13b H133A/H1058A4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gtccaacaagagcgtggccatggatatgctcaacgaagtgaagcggtgccccgacgagctgttcacaacactgtctgccgagaagcagtcccggttcagaatcatcagcgacgaccacaatgaagtgctgatgaagcggagcagcgacagattcgtgcctctgctgctgcagtatcaggttgttctcgcaccggtacctatacgagttgcttcacttcgccacggggctgctcgacaagtgttgtgacagacggctcttcgtcagggccaagtcttagtagtcgctgctggtgttacttcacgactacttcgcctcgtcgctgtctaagcacggagacgacgacgtcataS286NK288SV290AM292DM294LN296EV298KR300CP302DE304LF306TT308LS310AE312KQ314SR316FR318II320SD322DH324NE326VL328MK330RS332SD334RF336VP338LL340LQ342Y3872-7273dPspCas13b H133A/H1058A4,7404,7604,7804,8004,8204,8404,8604,8804,900
    atcgattacggcaagctgttcgaccacatcaggttccacgtgaacatgggcaagctgagatacctgctgaaggccgacaagacctgcatcgacggccagaccagagtcagagtgatcgagcagcccctgaacggcttcggcagactggaagaggccgagacaatgcggaagcaagtagctaatgccgttcgacaagctggtgtagtccaaggtgcacttgtacccgttcgactctatggacgacttccggctgttctggacgtagctgccggtctggtctcagtctcactagctcgtcggggacttgccgaagccgtctgaccttctccggctctgttacgccttcgttcPshAII344DY346GK348LF350DH352IR354FH356VN358MG360KL362RY364LL366KA368DK370TC372ID374GQ376TR378VR380VI382EQ384PL386NG388FG390RL392EE394AE396TM398RK400Q3872-7273dPspCas13b H133A/H1058A4,9204,9404,9604,9805,0005,0205,0405,060
    agaacggcaccttcggcaacagcggcatccggatcagagacttcgagaacatgaagcgggacgacgccaatcctgccaactatccctacatcgtggacacctacacacactacatcctggaaaacaacaaggtcgagatgtttatcaacgacaaagaggacagcgccccactgcttcttgccgtggaagccgttgtcgccgtaggcctagtctctgaagctcttgtacttcgccctgctgcggttaggacggttgatagggatgtagcacctgtggatgtgtgtgatgtaggaccttttgttgttccagctctacaaatagttgctgtttctcctgtcgcggggtgacgaBsaBIE402NG404TF406GN408SG410IR412IR414DF416EN418MK420RD422DA424NP426AN428YP430YI432VD434TY436TH438YI440LE442NN444KV446EM448FI450ND452KE454DS456AP458LL4603872-7273dPspCas13b H133A/H1058A5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gcccgtgatcgaggatgatagatacgtggtcaagacaatccccagctgccggatgagcaccctggaaattccagccatggccttccacatgtttctgttcggcagcaagaaaaccgagaagctgatcgtggacgtgcacaaccggtacaagagactgttccaggccatgcagaaacgggcactagctcctactatctatgcaccagttctgttaggggtcgacggcctactcgtgggacctttaaggtcggtaccggaaggtgtacaaagacaagccgtcgttcttttggctcttcgactagcacctgcacgtgttggccatgttctctgacaaggtccggtacgtctttPV462IE464DD466RY468VV470KT472IP474SC476RM478ST480LE482IP484AM486AF488HM490FL492FG494SK496KT498EK500LI502VD504VH506NR508YK510RL512FQ514AM516QK5183872-7273dPspCas13b H133A/H1058A5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gaagaagtgaccgccgagaatatcgccagcttcggaatcgccgagagcgacctgcctcagaagatcctggatctgatcagcggcaatgcccacggcaaggatgtggacgccttcatcagactgaccgtggacgacatgctgaccgacaccgagcggagaatcaagagattcaaggcttcttcactggcggctcttatagcggtcgaagccttagcggctctcgctggacggagtcttctaggacctagactagtcgccgttacgggtgccgttcctacacctgcggaagtagtctgactggcacctgctgtacgactggctgtggctcgcctcttagttctctaagttccEE520VT522AE524NI526AS528FG530IA532ES534DL536PQ538KI540LD542LI544SG546NA548HG550KD552VD554AF556IR558LT560VD562DM564LT566DT568ER570RI572KR574FK5763872-7273dPspCas13b H133A/H1058A5,4405,4605,4805,5005,5205,5405,5605,5805,600
    acgaccggaagtccattcggagcgccgacaacaagatgggaaagagaggcttcaagcagatctccacaggcaagctggccgacttcctggccaaggacatcgtgctgtttcagcccagcgtgaacgatggcgagaacaagatcaccggcctgaactaccggatcatgcagagcgctgctggccttcaggtaagcctcgcggctgttgttctaccctttctctccgaagttcgtctagaggtgtccgttcgaccggctgaaggaccggttcctgtagcacgacaaagtcgggtcgcacttgctaccgctcttgttctagtggccggacttgatggcctagtacgtctcgcgDD578RK580SI582RS584AD586NK588MG590KR592GF594KQ596IS598TG600KL602AD604FL606AK608DI610VL612FQ614PS616VN618DG620EN622KI624TG626LN628YR630IM632QS634A3872-7273dPspCas13b H133A/H1058A5,6205,6405,6605,6805,7005,7205,7405,760
    cattgccgtgtacgatagcggcgacgattacgaggccaagcagcagttcaagctgatgttcgagaaggcccggctgatcggcaagggcacaacagagcctcatccatttctgtacaaggtgttcgcccgcagcatccccgccaatgccgtcgagttctacgagcgctacctgatcgtaacggcacatgctatcgccgctgctaatgctccggttcgtcgtcaagttcgactacaagctcttccgggccgactagccgttcccgtgttgtctcggagtaggtaaagacatgttccacaagcgggcgtcgtaggggcggttacggcagctcaagatgctcgcgatggactagAfeIAjuII636AV638YD640SG642DD644YE646AK648QQ650FK652LM654FE656KA658RL660IG662KG664TT666EP668HP670FL672YK674VF676AR678SI680PA682NA684VE686FY688ER690YL692I3872-7273dPspCas13b H133A/H1058A5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gagcggaagttctacctgaccggcctgtccaacgagatcaagaaaggcaacagagtggatgtgcccttcatccggcgggaccagaacaagtggaaaacacccgccatgaagaccctgggcagaatctacagcgaggatctgcccgtggaactgcccagacagatgttcgacaatgctcgccttcaagatggactggccggacaggttgctctagttctttccgttgtctcacctacacgggaagtaggccgccctggtcttgttcaccttttgtgggcggtacttctgggacccgtcttagatgtcgctcctagacgggcaccttgacgggtctgtctacaagctgttacPasIE694RK696FY698LT700GL702SN704EI706KK708GN710RV712DV714PF716IR718RD720QN722KW724KT726PA728MK730TL732GR734IY736SE738DL740PV742EL744PR746QM748FD750N3872-7273dPspCas13b H133A/H1058A5,9605,9806,0006,0206,0406,0606,0806,1006,120
    agatcaagtcccacctgaagtccctgccacagatggaaggcatcgacttcaacaatgccaacgtgacctatctgatcgccgagtacatgaagagagtgctggacgacgacttccagaccttctaccagtggaaccgcaactaccggtacatggacatgcttaagggcgagtacgatctagttcagggtggacttcagggacggtgtctaccttccgtagctgaagttgttacggttgcactggatagactagcggctcatgtacttctctcacgacctgctgctgaaggtctggaagatggtcaccttggcgttgatggccatgtacctgtacgaattcccgctcatgctE752IK754SH756LK758SL760PQ762ME764GI766DF768NN770AN772VT774YL776IA778EY780MK782RV784LD786DD788FQ790TF792YQ794WN796RN798YR800YM802DM804LK806GE808YD8103872-7273dPspCas13b H133A/H1058A6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cagaaagggctccctgcagcactgcttcaccagcgtggaagagagagaaggcctctggaaagagcgggcctccagaacagagcggtacagaaagcaggccagcaacaagatccgcagcaaccggcagatgagaaacgccagcagcgaagagatcgagacaatcctggataagcgggtctttcccgagggacgtcgtgacgaagtggtcgcaccttctctctcttccggagacctttctcgcccggaggtcttgtctcgccatgtctttcgtccggtcgttgttctaggcgtcgttggccgtctactctttgcggtcgtcgcttctctagctctgttaggacctattcgccAleIRK812GS814LQ816HC818FT820SV822EE824RE826GL828WK830ER832AS834RT836ER838YR840KQ842AS844NK846IR848SN850RQ852MR854NA856SS858EE860IE862TI864LD866KR8683872-7273dPspCas13b H133A/H1058A6,3206,3406,3606,3806,4006,4206,4406,460
    ctgagcaacagccggaacgagtaccagaaaagcgagaaagtgatccggcgctacagagtgcaggatgccctgctgtttctgctggccaaaaagaccctgaccgaactggccgatttcgacggcgagaggttcaaactgaaagaaatcatgcccgacgccgagaagggaatcctgagactcgttgtcggccttgctcatggtcttttcgctctttcactaggccgcgatgtctcacgtcctacgggacgacaaagacgaccggtttttctgggactggcttgaccggctaaagctgccgctctccaagtttgactttctttagtacgggctgcggctcttcccttaggactBlpILS870NS872RN874EY876QK878SE880KV882IR884RY886RV888QD890AL892LF894LL896AK898KT900LT902EL904AD906FD908GE910RF912KL914KE916IM918PD920AE922KG924IL9263872-7273dPspCas13b H133A/H1058A6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gcgagatcatgcccatgagcttcaccttcgagaaaggcggcaagaagtacaccatcaccagcgagggcatgaagctgaagaactacggcgacttctttgtgctggctagcgacaagaggatcggcaacctgctggaactcgtgggcagcgacatcgtgtccaaagaggatatcatcgctctagtacgggtactcgaagtggaagctctttccgccgttcttcatgtggtagtggtcgctcccgtacttcgacttcttgatgccgctgaagaaacacgaccgatcgctgttctcctagccgttggacgaccttgagcacccgtcgctgtagcacaggtttctcctatagtaBmtINheISE928IM930PM932SF934TF936EK938GG940KK942YT944IT946SE948GM950KL952KN954YG956DF958FV960LA962SD964KR966IG968NL970LE972LV974GS976DI978VS980KE982DI984M3872-7273dPspCas13b H133A/H1058A6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ggaagagttcaacaaatacgaccagtgcaggcccgagatcagctccatcgtgttcaacctggaaaagtgggccttcgacacataccccgagctgtctgccagagtggaccgggaagagaaggtggacttcaagagcatcctgaaaatcctgctgaacaacaagaacatcaacaaaccttctcaagttgtttatgctggtcacgtccgggctctagtcgaggtagcacaagttggaccttttcacccggaagctgtgtatggggctcgacagacggtctcacctggcccttctcttccacctgaagttctcgtaggacttttaggacgacttgttgttcttgtagttgtttE986EF988NK990YD992QC994RP996EI998SS1000IV1002FN1004LE1006KW1008AF1010DT1012YP1014EL1016SA1018RV1020DR1022EE1024KV1026DF1028KS1030IL1032KI1034LL1036NN1038KN1040IN1042K3872-7273dPspCas13b H133A/H1058A6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gagcagagcgacatcctgCggaagatccggaacgccttcgatGCAaacaattaccccgacaaaggcgtggtggaaatcaaggccctgcctgagatcgccatgagcatcaagaaggcctttggggagtacgccatcatgaagggatccCTTCAACTGCCTCCACTTGAAAGACTGActcgtctcgctgtaggacGccttctaggccttgcggaagctaCGTttgttaatggggctgtttccgcaccacctttagttccgggacggactctagcggtactcgtagttcttccggaaacccctcatgcggtagtacttccctaggGAAGTTGACGGAGGTGAACTTTCTGACTBamHIE1044QS1046DI1048LR1050KI1052RN1054AF1056DA1058NN1060YP1062DK1064GV1066VE1068IK1070AL1072PE1074IA1076MS1078IK1080KA1082FG1084EY1086AI1088MK1090GS1092LQ1094LP1096PL1098ER1100L3872-7273dPspCas13b H133A/H1058AHIV NESHEPN27,0207,0407,0607,0807,1007,1207,1407,160
    CACTGGGTAGTTCCtacccatacgatgttccagattacgcttatccctacgacgtgcctgattatgcatacccatatgatgtccccgactatgcctaaGaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactaGTGACCCATCAAGGatgggtatgctacaaggtctaatgcgaatagggatgctgcacggactaatacgtatgggtatactacaggggctgatacggattCttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatNdeIEcoRIT1102LG1104SS1106YP1108YD1110VP1112DY1114AY1116PY1118DV1120PD1122YA1124YP1126YD1128VP1130DY1132A*11343872-7273HA tag 3xWPRE7,1807,2007,2207,2407,2607,2807,3007,3207,340
    tgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgWPRE7,3607,3807,4007,4207,4407,4607,4807,5007,520
    gcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaPflMIWPRE7,5407,5607,5807,6007,6207,6407,6607,6807,700
    cctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggcggaaaggaaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccgWPRE7,7207,7407,7607,7807,8007,8207,8407,860
    cgcctccccgcatcgataccgtcgacctcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagcggaggggcgtagctatggcagctggagctctggatctttttgtacctcgttagtgttcatcgttatgtcgtcgatggttacgactaacacggaccgatcttcgtgttctcctcctcctccacccaaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtKpnITliIXhoIAcc65IBsu36IPaeR7IWPRE7,8807,9007,9207,9407,9607,9808,0008,0208,040
    gctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggtcgacatctagaatcggtgaaaaattttcttttcccccctgaccttcccgattaagtgagggttgcttctgttctataggaactagacacctagatggtgtgtgttccgatgaagggactaaccgtcttgatgtgtggtcccggtccctagtctataggtgactggaaacctacca8,0608,0808,1008,1208,1408,1608,1808,2008,220
    gctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtattagagtggaggtttgacagccgcctagcatttcatcacatggcccgagagctgcatcccgatgttcgatcatggtcaactcgttctcttccatcttcttcggttacttcctctcttgtgggcgaacaatgtgggacactcggacgtaccctacctactgggcctctctcttcataatctcacctccaaactgtcggcggatcgtaaagtagtgtaccgggctctcgacgtagg8,2408,2608,2808,3008,3208,3408,3608,3808,400
    ggactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgcctgacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcac3' LTR8,4208,4408,4608,4808,5008,5208,5408,560
    tggaaaatctctagcagggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggaccttttagagatcgtcccgggcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccApaIPmeIPspOMI3' LTR8,5808,6008,6208,6408,6608,6808,7008,7208,740
    tgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcggacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgccF1 ori8,7608,7808,8008,8208,8408,8608,8808,9008,920
    gtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaacacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggtttttF1 ori8,9408,9608,9809,0009,0209,0409,0609,0809,100
    acttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggAloIPsiIF1 ori9,1209,1409,1609,1809,2009,2209,2409,260
    tattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggataaccaattttttactcgactaaattgtttttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccSexAI9,2809,3009,3209,3409,3609,3809,4009,4209,440
    cagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaaggtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttc9,4609,4809,5009,5209,5409,5609,5809,6009,620
    tagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgttgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaaccatggccaagttgaccagtgccgtatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcacaactgttaattagtagccgtatcatatagccgtatcatattatgctgttccactccttgatttggtaccggttcaactggtcacggcaSmaIXmaIAvrIITspMI9,6409,6609,6809,7009,7209,7409,7609,7809,800
    tccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgaggccacgagtggcgcgcgctgcagcggcctcgccagctcaagacctggctggccgagcccaagagggccctgaagcacctcctgctgaagcggccacaccaggccctgctgcactgggacaagtagtcgcgccaggtcctggtccaccacggcctgttgtgggaccggacccacSmaIXmaIMauBISexAISgrAITspMI9,8209,8409,8609,8809,9009,9209,9409,960
    tgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggcgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagcaggactacccacgcgccggacctgctcgacatgcggctcaccagcctccagcacaggtgcttgaaggccctgcggaggcccggccggtactggctctagccgctcgtcggcacccccgccctcaagcgggacgcgctgggccggccgttgacgcacgtgaagcaccggctcctcgtcctgaEagIFseI9,98010,00010,02010,04010,06010,08010,10010,12010,140
    gacacgtgctacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagctgtgcacgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcPsiI10,16010,18010,20010,22010,24010,26010,28010,30010,320
    catcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgBsmIBstZ17ILacOM13-rev10,34010,36010,38010,40010,42010,44010,46010,48010,500
    aacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaalac promoter10,52010,54010,56010,58010,60010,62010,64010,660
    ccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgSapIBspQIColE...gin10,68010,70010,72010,74010,76010,78010,80010,82010,840
    gtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctccctcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaColE1 origin10,86010,88010,90010,92010,94010,96010,98011,00011,020
    tcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccColE1 origin11,04011,06011,08011,10011,12011,14011,16011,18011,200
    cagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttgColE1 origin11,22011,24011,26011,28011,30011,32011,34011,360
    caccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttgtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaColE1 origin11,38011,40011,42011,44011,46011,48011,50011,52011,540
    ttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccataatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtaAmpR11,56011,58011,60011,62011,64011,66011,68011,70011,720
    ctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattAmpR11,74011,76011,78011,80011,82011,84011,86011,88011,900
    tagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttgatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaacFspIPvuIAmpR11,92011,94011,96011,98012,00012,02012,04012,060
    gccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgScaIAmpR12,08012,10012,12012,14012,16012,18012,20012,22012,240
    atagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttAmpR12,26012,28012,30012,32012,34012,36012,38012,40012,420
    tgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccaacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtSspI12,44012,46012,48012,50012,52012,54012,56012,58012,600
    cctgacggactg12,605
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12606 bp9000100001100012000100020003000EF-1aQB0226 (DNA)QB0019 (DNA)QB0112 (DNA)QB0070 (DNA)CMV enhancer,SgrDI,NruI,MluI,SpeI,NdeI,SnaBI,CspCI5'LTR,KasI,NarI,SfoI,PluTINruIBspQI,SapI,EagI,NotIRRE,BbvCIAleI,BsmIKflIPacIAvrII,TspMI,XmaI,SmaI,MauBITspMI,XmaI,SmaI,SgrAI,SexAIFseI,EagIPsiI,BsmI,BstZ17IM13-rev,LacO,lac promoterBspQISapIColE1 originFspIAmpR,PvuI,ScaI,SspI

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    12606