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LSM101 CMV-TO-ADAR2dd(E488Q, T501A)-MCP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttapUC ori20406080100120140160
    gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcccgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggAhdIBsaIAmp (bla)180200220240260280300320340
    agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgAclIFspIAmp (bla)360380400420440460480500520
    tccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacAmp (bla)540560580600620640660680700
    tgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctagAclIBcgIScaIXmnIAmp (bla)720740760780800820840860880
    cagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcSspIAmp (bla)bla promoter9009209409609801,0001,0201,0401,060
    ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtaccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatSgrDIbla promoter1,0801,1001,1201,1401,1601,1801,2001,2201,240
    tctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacattgattattgactagttattaataagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtaactaataactgatcaataattatMfeIMluISpeIAlwNIBsiWIBpu10ICMVCMV promoter (with TetOs)1,2601,2801,3001,3201,3401,3601,3801,4001,420
    gtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcCMVCMV promoter (with TetOs)1,4401,4601,4801,5001,5201,5401,5601,5801,600
    tatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgaataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactNdeISnaBICMVCMV promoter (with TetOs)1,6201,6401,6601,6801,7001,7201,7401,7601,780
    tgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccacCspCICMVCMV promoter (with TetOs)1,8001,8201,8401,8601,8801,9001,9201,940
    ggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttPmeISacISacISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetOhomologfrom...me)1,9601,9802,0002,0202,0402,0602,0802,1002,120
    cttaagcttcgccaccATGTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGgaattcgaagcggtggTACAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACXcmIAflIIBseRIBstEIIHindIIIML2HL4DQ6TP8SR10QP12IP14SE16GL18QL20HL22PQ24VL26AD28AV30SR32LV34LG36KF38GD40LT42DN44FS46SP48HA50RR52KV542153-4123hADAR2 cat domainfrom original...digest same)2,1402,1602,1802,2002,2202,2402,2602,2802,300
    CTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGLA56GV58VM60TT62GT64DV66KD68AK70VI72SV74ST76GT78KC80IN82GE84YM86SD88RG90LA92LN94DC96HA98EI100IS102RR104SL106LR108FL110YT112Q2153-4123hADAR2 cat domain2,3202,3402,3602,3802,4002,4202,4402,4602,480
    TTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCBclIPfoIBsrGIL114EL116YL118NN120KD122DQ124KR126SI128FQ130KS132ER134GG136FR138LK140EN142VQ144FH146LY148IS150TS152PC154GD156AR158IF160SP162HE164PI166LE168EP170AD172R2153-4123hADAR2 cat domain2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCBcgIPstIH174PN176RK178AR180GQ182LR184TK186IE188SG190QG192TI194PV196RS198NA200SI202QA204WD206GV208LQ210GE212RL214LT216MS218CS220DK222IA224RW226NV228VG230IQ2322153-4123hADAR2 cat domainC-term ADAR22,6802,7002,7202,7402,7602,7802,8002,8202,840
    GGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCAvaIBlpITliIXhoIBamHIBsoBIPaeR7IGS234LL236SI238FV240EP242IY244FS246SI248IL250GS252LY254HG256DH258LS260RA262MY264QR266IS268NI270ED272LP274PL276YT278LN280KP282LL284SG286IS288NA290E2153-4123hADAR2 cat domainC-term ADAR22,8602,8802,9002,9202,9402,9602,9803,0003,020
    CACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGAMluIA292RQ294PG296KA298PN300FS302VN304WT306VG308DS310AI312EV314IN316AT318TG320KD322EL324GR326AS328RL330CK332HA334LY336CR338WM340RV342HG344KV346PS348HL350L2153-4123hADAR2 cat domainC-term ADAR23,0403,0603,0803,1003,1203,1403,1603,1803,200
    ACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCGGTACCGGTGGCGGAACCTGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGCCATGGCCACCGCCTTGGKpnIAcc65IGT2GG4GT63365-4120R352SK354IT356KP358NV360YH362ES364KL366AA368KE370YQ372AA374KA376RL378FT380AF382IK384AG386LG388AW390VE392KP394TE396QD398QF400SL402TP404GT406GG408GT4102153-4123G1T2G3G4G5T63365-3394hADAR2 cat domainC-term ADAR2short linkerLSM093...m 94)3,2203,2403,2603,2803,3003,3203,3403,3603,380
    GGTGGCGGGGCTATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGACCACCGCCCCGATACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTGG8GA10MA12SN14FT16QF18VL20VD22NG24GT26GD28VT30VA32PS34NF36AN38GI40AE42WI44SS46NS48RS50QA52YK54VT56CS58VR60QS62SA64Q3365-4120GG412GA414MA416SN418FT420QF422VL424VD426NG428GT430GD432VT434VA436PS438NF440AN442GI444AE446WI448SS450NS452RS454QA456YK458VT460CS462VR464QS466SA468Q2153-4123G7G8G9A103365-3394LSM093 (rest from 94)MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPhomologsho...er3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ACCGAAAATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGTGGCTTTTATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCXcmIAsiSIBstEIIN66RK68YT70IK72VE74VP76KG78AW80RS82YL84NM86EL88TI90PI92FA94TN96SD98CE100LI102VK104AM106QG108LL110KD112GN114PI116PS118AI120AA122NS124G3365-4120N470RK472YT474IK476VE478VP480KG482AW484RS486YL488NM490EL492TI494PI496FA498TN500SD502CE504LI506VK508AM510QG512LL514KD516GN518PI520PS522AI524AA526NS528G2153-4123LSM093 (rest from 94)MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCP3,5803,6003,6203,6403,6603,6803,7003,720
    AATATATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTATTATATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATBplIBplII126YG128GG130TG132GG134SM136AS138NF140TQ142FV144LV146DN148GG150TG152DV154TV156AP158SN160FA162NG164IA166EW168IS170SN172SR174SQ176AY178KV180TC182SV1843365-4120I530YG532GG534TG536GG538SM540AS542NF544TQ546FV548LV550DN552GG554TG556DV558TV560AP562SN564FA566NG568IA570EW572IS574SN576SR578SQ580AY582KV584TC586SV5882153-4123LSM093 (rest from 94)from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP3,7403,7603,7803,8003,8203,8403,8603,8803,900
    CGCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGGCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCBstXIBstZ17IRQ186SS188AQ190NR192KY194TI196KV198EV200PK202GA204WR206SY208LN210ME212LT214IP216IF218AT220NS222DC224EL226IV228KA230MQ232GL234LK236DG238NP240IP242S3365-4120RQ590SS592AQ594NR596KY598TI600KV602EV604PK606GA608WR610SY612LN614ME616LT618IP620IF622AT624NS626DC628EL630IV632KA634MQ636GL638LK640DG642NP644IP646S2153-4123LSM093 (rest from 94)from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP3,9203,9403,9603,9804,0004,0204,0404,0604,080
    CAATCGCTGCGAATAGCGGAATTTACtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcGTTAGCGACGCTTATCGCCTTAAATGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagApaIAvaIBclIPmeITliIXhoIBseRIBsoBIPspXIPaeR7IPspOMIA244IA246AN248SG250IY2523365-4120A648IA650AN652SG654IY656*2153-4123*1BGH polyadenylation sequencec-myc epitopeLSM093 (rest from 94)from pCDH ...zak_tdMCPMCPPolyhi...e tag4,1004,1204,1404,1604,1804,2004,2204,2404,260
    ccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggcggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgSphIBGH polyadenylation sequence4,2804,3004,3204,3404,3604,3804,4004,4204,440
    tctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcClaIBspDIBGH pA4,4604,4804,5004,5204,5404,5604,5804,6004,620
    ctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagaKflINaeINgoMIVFRT4,6404,6604,6804,7004,7204,7404,7604,7804,800
    ctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatBarIMscIXbaIPshAIHygromycinFRT4,8204,8404,8604,8804,9004,9204,9404,9604,980
    gctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgtcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaEcoRIDraIIIHygromycin5,0005,0205,0405,0605,0805,1005,1205,1405,160
    tctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagNdeIPstIAsiSIRsrIIHygromycin5,1805,2005,2205,2405,2605,2805,3005,3205,340
    agtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcDraIIIHygromycin5,3605,3805,4005,4205,4405,4605,4805,500
    ccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgAjuISacIIHygromycin5,5205,5405,5605,5805,6005,6205,6405,6605,680
    gcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttccgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagScaIHygromycin5,7005,7205,7405,7605,7805,8005,8205,8405,860
    gattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcNaeIPfoIPsiINgoMIVSV40 pA5,8805,9005,9205,9405,9605,9806,0006,0206,040
    catttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtggtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacBsmIBstZ17ISV40 pA6,0606,0806,1006,1206,1406,1606,1806,2006,220
    taaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgcatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgSapIBspQI6,2406,2606,2806,3006,3206,3406,3606,3806,400
    tcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgPciIpUC ori6,4206,4406,4606,4806,5006,5206,5406,5606,580
    atcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacpUC ori6,6006,6206,6406,6606,6806,7006,7206,7406,760
    taggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccataAlwNIpUC ori6,7806,8006,8206,8406,8606,8806,9006,9206,940
    gtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctpUC ori6,9606,9807,0007,0207,0407,0607,0807,1007,120
    ttacgcgcagaaaaaaaggatctcaaaatgcgcgtctttttttcctagagttpUC ori7,1307,140
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7146 bp500055006000650050010001500CMVEKo152.2 (DNA)CMVF1 (DNA)YXo025.1 (DNA)pNSK310 (DNA)oLSM0140 (DNA)XZp025.1 (DNA)NK164-RBPMS-A-for (DNA)EKo272.2 (DNA)EKbF (DNA)YH029.1 (DNA)CCp080 Cpep-F (DNA)CCp027-AV373Kozak-f (DNA)p450_F (DNA)oLSM0262 (DNA)EWo100.1 (DNA)pNSK174 (DNA)oLSM0261 (DNA)EKo330.4 (DNA)XZ046-test-R (DNA)oLSM0113 (DNA)XZp053.2 (DNA)oLSM0294 (DNA)oLSM0115 (DNA)EKo329.5 (DNA)XZp021.4 (DNA)XZp064.1 (DNA)XZp009.3 (DNA)MCP-ADAR-seq-F0 (DNA)MCP-ADAR1-seq-F1 (DNA)oLSM0257 (DNA)oLSM0139 (DNA)oLSM0288 (DNA)EKo299.3 (DNA)EKo152.1 (DNA)Hyg_F (DNA)SV40PA_R (DNA)An-o17 U6_3MS2_Cre_seq (DNA)pUCori5F (DNA)oLSM-pUC_ori_F (DNA)oLSM-pUC_ori_R (DNA)pUCoriR (DNA)AhdI,BsaIFspIAclIAmp (bla)ScaIBcgIAclIXmnISspIbla promoterSgrDIAlwNIMfeI,Bpu10IBsiWI,MluISpeINdeISnaBICMV promoter (with TetOs)DraIIIAjuISacIIScaIPfoI,NgoMIV,NaeIPsiISV40 pABsmIBstZ17IBspQISapIPciIpUC oriAlwNI

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    7146