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sgRNA PCR-v2
  • Sequence Map
    Editing disabled on read-only sequence.
    AATGATACGGCGACCACCGAGATCTACACNNNNNNNNNNACACTCTTTCCCTACACGACGCTCTTCCGATCTgaaagtatttcgatttcttggctttatatatcttGTGGAAAGGACGAAACACCGNNNNNNNNNNNNNNNNNNNgtttaagagctaagctggaaacagcatagcTTACTATGCCGCTGGTGGCTCTAGATGTGNNNNNNNNNNTGTGAGAAAGGGATGTGCTGCGAGAAGGCTAGActttcataaagctaaagaaccgaaatatatagaaCACCTTTCCTGCTTTGTGGCNNNNNNNNNNNNNNNNNNNcaaattctcgattcgacctttgtcgtatcgAluIAluIBarIBlpIDdeIEarIHgaIMseISapITaqIBceAIBglIIBspQIBstYILpnPITaqαIHpy99IPCR vector sequence10X TT set A P5read 2 sequence (92 bps)Fwd-v2TrueSeq Read 1 P5Read 1 sequence (26)sgRNA20406080100120140160
    aagtttaaataaggctagtccgttatcaacttAGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNNNNNATCTCGTATGCCGTCTTCTGCTTGttcaaatttattccgatcaggcaatagttgaaTCTAGCCTTCTCGTGTGCAGACTTGAGGTCAGTGNNNNNNNNNNTAGAGCATACGGCAGAAGACGAACBbsIBfaIBpmIBsrIDdeIDraIEarIMseISapIBceAIBmgBIBspQILpnPIAflIIITsp45IBsiHKAIBsp1286IHpyCH4IVrev-AS3TrueSeq Read2PCR vector sequenceread 2 sequence (92 bps)Capturein...)10X TT set A P7P7180200220240260
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    Editing disabled on read-only sequence.
    275 bp2002252502550PCR vector sequenceFwd-v2rev-AS3CaptureP5P7BarIBglIIBstYI,BceAI10X TT set A P5TrueSeq Read 1 Hpy99IHgaIBspQI,EarI,SapIBspQIEarI,SapIBpmIAflIII,BsiHKAI,Bsp1286I,HpyCH4IVBmgBITrueSeq Read2BsrITsp45I10X TT set A P7,index(i7),BceAI,LpnPIBbsI

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