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LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggBspHI20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttBsrDIAmp180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspIBsrDIAmp360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattAmp540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIAmp720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataAmp8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaBspHICspCI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggAloI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcatt1,4201,4401,4601,4801,5001,5201,5401,560
    ccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagFspI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatatttgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataaBII(R)5'TR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttBsiWI31bp spacer5'TR(1)5'TR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgccccore insul2,1202,1402,1602,1802,2002,2202,2402,260
    gacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagacccctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctgggMfeISfiISrfIcore insulSFFV2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cacctgtaggttatggcaagctagctgcagtaacgccattattgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacgtggacatccaataccgttcgatcgacgtcattgcggtaataacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgBmtINheIEcoRVSFFV2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agatggtcaccgcagtttcggccccggcccgaggccaagagcagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttctaccagtggcgtcaaagccggggccgggctccggttctcgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaAflIIBbvCISFFV2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcgcgcgcttctgcttcccgagctctataaaagagctcacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGagcgcgcgaagacgaagggctcgagatattttctcgagtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCAscIHindIIIM1VS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG242905-3612SFFVmCherryhomo...ch)2,8202,8402,8602,8802,9002,9202,9402,960
    CTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACGAGGCACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGSV26NG28HE30FE32IE34GE36GE38GR40PY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD822905-3612mCherry2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ATTCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCTAAGGGCTGATGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGAloISbfIIP84DY86LK88LS90FP92EG94FK96WE98RV100MN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM1402905-3612mCherry3,1603,1803,2003,2203,2403,2603,2803,3003,320
    AGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTTCTTCTTCTGGTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAKasIKasINarINarISfoISfoIBbvCIPflMIPluTIPluTIQK142KT144MG146WE148AS150SE152RM154YP156ED158GA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198V2905-3612mCherry3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGTTGTAGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAAMluIXcmIBsrGISgrAIAflIIID1A2L3E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F183622-3720N200IK202LD204IT206SH208NE210DY212TI214VE216QY218ER220AE222GR224HS226TG228GM230DE232LY234KS2362905-3612mCherry5' StopGo (2A; Loughran 2017)3,5203,5403,5603,5803,6003,6203,6403,660
    GACCTTCTGAAGCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtaCTGGAAGACTTCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatAgeIBaeIKpnIZraIAatIIBamHIAcc65IS1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T373733-3843G1T2G1S2D19L20L21K22L23A24G25D26V27E28S29N30P31G32P333622-3720VG2GI4VT6YT8IR10VR12LR14HH16QG18LS20GT22AS24AY26HS28IP30PT32QT34TG363742-5922T1G2ADAR substrate3xMS2 SGR5' StopGo (2A; Loughran 2017)5' stem3' stem3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ctagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTggagtgcaggtggagactatctccccaggagacgggcgcaccttccccaagcgcggccagacctggatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCAcctcacgtccacctctgatagaggggtcctctgcccgcgtggaaggggttcgcgccggtctggacSpeITliIXhoIPspXIPaeR7IT1S2GV2QV4ET6IS8PG10DG12RT14FP16KR18GQ20TC223961-4281E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P333856-3954TS38EA40RH42KQ44KI46VA48PV50KQ52TL54NF56DL58LK60LA62GD64VE66SN68PG70PS72SG74VQ76VE78TI80SP82GD84GR86TF88PK90RG92QT94C3742-59223' StopGo (2A; Loughran 2017)DimmerizationDmrB3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cgtggtgcactacaccgggatgcttgaagatggaaagaaagttgattcctcccgggacagaaacaagccctttaagtttatgctaggcaagcaggaggtgatccgaggctgggaagaaggggttgcccagatgagtgtgggtcagagagccaaactgactatatctccagattatgcaccacgtgatgtggccctacgaacttctacctttctttcaactaaggagggccctgtctttgttcgggaaattcaaatacgatccgttcgtcctccactaggctccgacccttcttccccaacgggtctactcacacccagtctctcggtttgactgatatagaggtctaataVV24HY26TG28ML30ED32GK34KV36DS38SR40DR42NK44PF46KF48ML50GK52QE54VI56RG58WE60EG62VA64QM66SV68GQ70RA72KL74TI76SP78DY803961-4281V96VH98YT100GM102LE104DG106KK108VD110SS112RD114RN116KP118FK120FM122LG124KQ126EV128IR130GW132EE134GV136AQ138MS140VG142QR144AK146LT148IS150PD152Y3742-5922DimmerizationDmrB4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gcctatggtgccactgggcacccaggcatcatcccaccacatgccactctcgtcttcgatgtggagcttctaaaactggaatctggcggtggatccggagtcgacggatttggtgatgtcggtgctcttgagagtttgaggggaaatgcagatttggcttacatcctgagcatggcggataccacggtgacccgtgggtccgtagtagggtggtgtacggtgagagcagaagctacacctcgaagattttgaccttagaccgccacctaggcctcagctgcctaaaccactacagccacgagaactctcaaactcccctttacgtctaaaccgaatgtaggactcgtaccBamHIAY82GA84TG86HP88GI90IP92PH94AT96LV98FD100VE102LL104KL106E3961-4281A154YG156AT158GH160PG162II164PP166HA168TL170VF172DV174EL176LK178LE180SG182GG184SG186VD188GF190GD192VG194AL196ES198LR200GN202AD204LA206YI208LS210M3742-5922DimmerizationDmrBiCasp94,2204,2404,2604,2804,3004,3204,3404,360
    agccctgtggccactgcctcattatcaacaatgtgaacttctgccgtgagtccgggctccgcacccgcactggctccaacatcgactgtgagaagttgcggcgtcgcttctcctcgctgcatttcatggtggaggtgaagggcgacctgactgccaagaaaatggtgctggcttttcgggacaccggtgacggagtaatagttgttacacttgaagacggcactcaggcccgaggcgtgggcgtgaccgaggttgtagctgacactcttcaacgccgcagcgaagaggagcgacgtaaagtaccacctccacttcccgctggactgacggttcttttaccacgaccgaaaE212PC214GH216CL218II220NN222VN224FC226RE228SG230LR232TR234TG236SN238ID240CE242KL244RR246RF248SS250LH252FM254VE256VK258GD260LT262AK264KM266VL268AL2703742-5922iCasp94,3804,4004,4204,4404,4604,4804,5004,5204,540
    gctggagctggcgcggcaggaccacggtgctctggactgctgcgtggtggtcattctctctcacggctgtcaggccagccacctgcagttcccaggggctgtctacggcacagatggatgccctgtgtcggtcgagaagattgtgaacatcttcaatgggaccagctgccccagccgacctcgaccgcgccgtcctggtgccacgagacctgacgacgcaccaccagtaagagagagtgccgacagtccggtcggtggacgtcaagggtccccgacagatgccgtgtctacctacgggacacagccagctcttctaacacttgtagaagttaccctggtcgacggggtcgQ4L3E2W14633-4644LE272LA274RQ276DH278GA280LD282CC284VV286VI288LS290HG292CQ294AS296HL298QF300PG302AV304YG306TD308GC310PV312SV314EK316IV318NI320FN322GT324SC326PS3283742-5922iCasp9WEL...te4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ctgggagggaagcccaagctctttttcatccaggcctgtggtggggagcagaaagaccatgggtttgaggtggcctccacttcccctgaagacgagtcccctggcagtaaccccgagccagatgccaccccgttccaggaaggtttgaggaccttcgaccagctggacgccatatgaccctcccttcgggttcgagaaaaagtaggtccggacaccacccctcgtctttctggtacccaaactccaccggaggtgaaggggacttctgctcaggggaccgtcattggggctcggtctacggtggggcaaggtccttccaaactcctggaagctggtcgacctgcggtataAjuIXcmILG330GK332PK334LF336FI338QA340CG342GE344QK346DH348GF350EV352AS354TS356PE358DE360SP362GS364NP366EP368DA370TP372FQ374EG376LR378TF380DQ382LD384AI3863742-5922iCasp94,7404,7604,7804,8004,8204,8404,8604,8804,900
    ctagtttgcccacacccagtgacatctttgtgtcctactctactttcccaggttttgtttcctggagggaccccaagagtggctcctggtacgttgagaccctggacgacatctttgagcagtgggctcactctgaagacctgcagtccctcctgcttagggtcgctaatgctgtgatcaaacgggtgtgggtcactgtagaaacacaggatgagatgaaagggtccaaaacaaaggacctccctggggttctcaccgaggaccatgcaactctgggacctgctgtagaaactcgtcacccgagtgagacttctggacgtcagggaggacgaatcccagcgattacgacaKflISS388LP390TP392SD394IF396VS398YS400TF402PG404FV406SW408RD410PK412SG414SW416YV418ET420LD422DI424FE426QW428AH430SE432DL434QS436LL438LR440VA442NA444V3742-5922iCasp94,9204,9404,9604,9805,0005,0205,0405,060
    ttcggtgaaagggatttataaacagatgcctggttgctttaatttcctccggaaaaaacttttctttaaaacatcaGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGTCTCAAAGGGCGAGGAACTTTTTACGGGCGTTGTTCCGATCaagccactttccctaaatatttgtctacggaccaacgaaattaaaggaggccttttttgaaaagaaattttgtagtCGGTGCTTGAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCAGAGTTTCCCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGBmgBIVS2KG4EE6LF8TG10VV12PI145209-5922S446VK448GI450YK452QM454PG456CF458NF460LR462KK464LF466FK468TS470AT472NF474SL476LK478QA480GD482VE484EN486PG488PV490SK492GE494EL496FT498GV500VP502I3742-5922iCasp9P2AGFP5,0805,1005,1205,1405,1605,1805,2005,2205,240
    CTCGTCGAATTGGACGGGGACGTTAATGGGCATAAATTCTCAGTTAGTGGTGAGGGTGAGGGGGACGCGACATATGGGAAACTTACGCTGAAGTTCATATGTACCACCGGAAAATTGCCAGTGCCGTGGCCTACTCTCGTCACCACCCTGACATATGGTGTTCAGTGTTTTAGCCGAGCAGCTTAACCTGCCCCTGCAATTACCCGTATTTAAGAGTCAATCACCACTCCCACTCCCCCTGCGCTGTATACCCTTTGAATGCGACTTCAAGTATACATGGTGGCCTTTTAACGGTCACGGCACCGGATGAGAGCAGTGGTGGGACTGTATACCACAAGTCACAAAATCGGLV16EL18DG20DV22NG24HK26FS28VS30GE32GE34GD36AT38YG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS725209-5922L504VE506LD508GD510VN512GH514KF516SV518SG520EG522EG524DA526TY528GK530LT532LK534FI536CT538TG540KL542PV544PW546PT548LV550TT552LT554YG556VQ558CF560S3742-5922GFP5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GGTATCCAGACCATATGAAACAGCACGATTTTTTTAAATCCGCTATGCCTGAAGGTTACGTACAGGAGCGAACGATATTTTTTAAAGACGACGGAAACTACAAAACTAGAGCCGAGGTAAAATTCGAGGGTGACACTCTCGTGAACCGGATCGAACTGAAGGGCATAGATTTTAACCATAGGTCTGGTATACTTTGTCGTGCTAAAAAAATTTAGGCGATACGGACTTCCAATGCATGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTTTGATGTTTTGATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAGCACTTGGCCTAGCTTGACTTCCCGTATCTAAAATTBarISnaBIRY74PD76HM78KQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI98FF100KD102DG104NY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130K5209-5922R562YP564DH566MK568QH570DF572FK574SA576MP578EG580YV582QE584RT586IF588FK590DD592GN594YK596TR598AE600VK602FE604GD606TL608VN610RI612EL614KG616ID618FK6203742-5922GFP5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GGAGGATGGCAATATACTGGGCCACAAGCTCGAATACAACTATAATTCTCATAATGTCTATATCATGGCGGACAAACAGAAAAACGGCATCAAGGTAAACTTTAAAATCCGACACAATATTGAGGACGGCTCTGTCCAGCTTGCGGACCATTACCAACAGAACACGCCGATAGGGCCTCCTACCGTTATATGACCCGGTGTTCGAGCTTATGTTGATATTAAGAGTATTACAGATATAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCATTTGAAATTTTAGGCTGTGTTATAACTCCTGCCGAGACAGGTCGAACGCCTGGTAATGGTTGTCTTGTGCGGCTATCCCE132DG134NI136LG138HK140LE142YN144YN146SH148NV150YI152MA154DK156QK158NG160IK162VN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188G5209-5922ED622GN624IL626GH628KL630EY632NY634NS636HN638VY640IM642AD644KQ646KN648GI650KV652NF654KI656RH658NI660ED662GS664VQ666LA668DH670YQ672QN674TP676IG6783742-5922GFP5,6205,6405,6605,6805,7005,7205,7405,760
    GATGGTCCAGTACTTCTCCCTGATAATCACTATTTGTCTACTCAGTCAGCTCTTAGCAAGGACCCAAACGAAAAACGGGATCACATGGTCCTTCTTGAGTTCGTCACTGCGGCTGGGATAACACTCGGGATGGACGAACTGTATAAGtaggccgctcgaggagggcccgaacaaaCTACCAGGTCATGAAGAGGGACTATTAGTGATAAACAGATGAGTCAGTCGAGAATCGTTCCTGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAACTCAAGCAGTGACGCCGACCCTATTGTGAGCCCTACCTGCTTGACATATTCatccggcgagctcctcccgggcttgtttApaITliIXhoIPspXIPaeR7IPspOMI*1D190GP192VL194LP196DN198HY200LS202TQ204SA206LS208KD210PN212EK214RD216HM218VL220LE222FV224TA226AG228IT230LG232MD234EL236YK2385209-5922DG680PV682LL684PD686NH688YL690ST692QS694AL696SK698DP700NE702KR704DH706MV708LL710EF712VT714AA716GI718TL720GM722DE724LY726K3742-5922GFPhomolog5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctattgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggatBclIPmeIBGH polyadenylation sequencec-myc epitopePolyhi...e tag5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggatattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctNotIBGH polyadenylation sequence5, 299 F6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactcttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgagSexAIpSV406,3206,3406,3606,3806,4006,4206,4406,460
    cgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtBclIAvrIIpSV406,4806,5006,5206,5406,5606,5806,6006,6206,640
    gcacgtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtcgtgcactactttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaPmlIZraIAatIIPshAIMK2KP4EL6TA8TS10VE12KF14LI16EK18FD20SV22SD24LM26QL28SE30GE32ES34RA36FS38FD40VG42GR44GY46VL48RV50NS52CA54DG566658-7683Hygro6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccEcoRIFY58KD60RY62VY64RH66FA68SA70AL72PI74PE76VL78DI80GE82FS84ES86LT88YC90IS92RR94AQ96GV98TL100QD102LP104ET106EL108PA110VL112QP1146658-7683Hygro2, 563 F6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtcagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcagAsiSIVA116EA118MD120AI122AA124AD126LS128QT130SG132FG134PF136GP138QG140IG142QY144TT146WR148DF150IC152AI154AD156PH158VY160HW162QT164VM166DD168TV170SA172S6658-7683Hygro7,0207,0407,0607,0807,1007,1207,1407,160
    cgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacgcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgV174AQ176AL178DE180LM182LW184AE186DC188PE190VR192HL194VH196AD198FG200SN202NV204LT206DN208GR210IT212AV214ID216WS218EA220MF222GD224SQ226YE228VA230N6658-7683Hygro7,1807,2007,2207,2407,2607,2807,3007,3207,340
    atcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgctagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgAjuISacIII232FF234WR236PW238LA240CM242EQ244QT246RY248FE250RR252HP254EL256AG258SP260RL262RA264YM266LR268IG270LD272QL274YQ276SL278VD280GN282FD284DA286AW288A6658-7683Hygro3, 135 F7,3607,3807,4007,4207,4407,4607,4807,5007,520
    agggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataggtcgacaatcaacctcttcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatccagctgttagttggagaQ290GR292CD294AI296VR298SG300AG302TV304GR306TQ308IA310RR312SA314AV316WT318DG320CV322EV324LA326DS328GN330RR332PS334TR336PR338AK340E*3426658-7683Hygro6, 730 RWPRE7,5407,5607,5807,6007,6207,6407,6607,6807,700
    ggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattcctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaHpaIWPRESV40 pASV40 pA(1)7,7207,7407,7607,7807,8007,8207,8407,860
    tttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttcccaaactgggttcttaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaagggtttgacccaagaatBsmISV40 pASV40 pA(1)7,8807,9007,9207,9407,9607,9808,0008,0208,040
    tatcccttgctctggtcaaccaggttgcagggtttcctgtcctcacaggaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggctcaaagagcaatagggaacgagaccagttggtccaacgtcccaaaggacaggagtgtccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtccgagtttctcgtApaIEcoNISexAIPspOMIcore insul(1) Gene...me: core insul(1)8,0608,0808,1008,1208,1408,1608,1808,2008,220
    gcgagaagcgttcagaggaaagcgatcccgtgccaccttccccgtgcccgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggggctttgggcgctcttcgcaagtctcctttcgctagggcacggtggaaggggcacgggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccccgaaacccSrfIcore insul(1) Geneious name: core insul(1)8,2408,2608,2808,3008,3208,3408,3608,3808,400
    ggggggctgtccctgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacccccccgacagggactatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgEcoRVcore...(1)8,4208,4408,4608,4808,5008,5208,5408,560
    ttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagBsmI3'TR8,5808,6008,6208,6408,6608,6808,7008,7208,740
    gtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagccc8,7608,7808,8008,8208,8408,8608,8808,9008,920
    aaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtc8,9408,9608,9809,0009,0209,0409,0609,0809,100
    cggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggcpLac(F)9,1209,1409,1609,1809,2009,2209,2409,260
    gaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgaSapIBspQI9,2809,3009,3209,3409,3609,3809,4009,4209,440
    cactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatagggtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccPciIAflIII9,4609,4809,5009,5209,5409,5609,5809,6009,620
    ctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtgggaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcacc9,6409,6609,6809,7009,7209,7409,7609,7809,800
    cgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactgggcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgacc9,8209,8409,8609,8809,9009,9209,9409,960
    taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcg9,98010,00010,02010,04010,06010,08010,10010,12010,140
    ggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagtt10,16010,18010,200
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10204 bp750080008500900095005001000150020002500HygroSFFVmissing in pSLQ2349SV40 pAMluI-PGK-GFAmp6658-7683oLSM0054 (DNA)EK_SFFV_F (DNA)YXo023.1 (DNA)YXo021.2 (DNA)XZp042.5 (DNA)pNSK126 (DNA)EKo387.3 (DNA)oLSM0072 (DNA)oLSM0057 (DNA)oLSM0068 (DNA)bghF (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),BspHI,BsrDIFspIAclI,BsrDIAclIoLSM0053 (DNA),BspHICspCIAloIFrtF (DNA)FlpInR (DNA)FspIM13-40FOR Genewiz (DNA),M13F Genewiz (DNA)BII(R)5'TRBsiWI31bp spacer5'TR(1),5'TR,5'TRcore insul,ES003-1 C->A,SrfIAdded MluI site,Gibson overlap,+5missing in pSLQ2349,EKo326.1 (DNA),+3BmtIHyg_F (DNA)6, 730 R,SalIWPRE,SV40 pA(1),SV40 pA(1),SV40 pA(1),+3SV40PA_R (DNA)SV40pA-R Genewiz (DNA)SV40 pA(1),BsmISexAIEcoNI,PspOMI,ApaIEWXglobF NONSPECIFIC DONT USE (DNA)core insul(1) Geneious name: core insul(1),+1EcoRVBsmI3'TRES003-3 C->AM13R Genewiz (DNA)pLac(F)pUCori5F (DNA),BspQI,SapI,AflIII,PciIpUCoriR (DNA)

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    10204

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