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pRS138 Addgene
  • Sequence Map
    Editing disabled on read-only sequence.
    tttatcgataagcttgggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccaaaatagctattcgaaccctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtClaIBspDICMV promoterCMV enhancer20406080100120140160
    cttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgNdeIBsaAICspCISnaBICMV promoterCMV enhancerCMV promoter180200220240260280300320340
    atcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataatagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattCMV promoterCMV promoter360380400420440460480500520
    gcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgactctactagaggatctatttccggtgaattcccgagcgtgtcagggtgaccgtggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctcgtctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggctgagatgatctcctagataaaggccacttaagggctcgcacagtcccactggcaccactcgttcccgctcctcgacaagtggccccaccacgggtaggaEcoRIBstEIICMV promoterRegion to be replaced with BC fragmenttruncated GFP (filler)BC-C1 (updag)540560580600620640660680700
    ggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgcccagctcgacctgccgctgcatttgccggtgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggactggatgccgcacgtcacgaagtcggcgRegion to be replaced with BC fragmenttruncated GFP (filler)EGFP-N720740760780800820840860
    taccccgaccacatgaagctctagagtattaccatggtcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaggatccaattctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggccatggggctggtgtacttcgagatctcataatggtaccagttttttttttttttttttttttttttttttcctaggttaagatggcccatcccctccgcgaaaagggttccgtcagacctcgtacgcgaaatcgtcggggcgacccgtgaaccgcgatgtgttcaccggagaccggNspISphIXbaIBamHIPGK promoterpolyA(30)Region...gmenttrunca...ller)8809009209409609801,0001,0201,040
    tcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcactagtctcgtgcagatggacagcgtgtgtaaggtgtaggtggccatccgcggttggccgaggcaagaaaccaccggggaagcgcggtggaagatgaggaggggatcagtccttcaagggggggcggggcgtcgagcgcagcacgtcctgcactgtttaccttcatcgtgcagagtgatcagagcacgtctacctgAgeISpeIPGK promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    agcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcatcgtggcgactcgttaccttcgcccatccggaaaccccgtcgccggttatcgtcgaaacgaggaagcgaaagacccgagtctccgacccttccccacccaggcccccgcccgagtccccgcccgagtccccgccccgcccgcgggcttccaggaggcctccgggccgtaagacgtBlpIBspEIPpuMIEcoO109IPGK promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctgcagttaacgccgccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccagcgaagttttcgcgtgcagacggcgcgacaagaggagaaggagtagaggcccggaaagctggacgtcaattgcggcggtactggctcatgttcgggtgccacgcggagcggtgggcgctgctgcagggggcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtHpaIPstISmaIXmaIBfuAIBsiWIBspMIPflFITspMITth111IPuroRPGK promoter1,4201,4401,4601,4801,5001,5201,5401,560
    cgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatgcgcggtgtggcagctgggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccacacccagcgcctgctgccgcggcgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctaRsrIISacIIBstEIIPuroR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgcaccaaggaccggtggcagccgcagagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctcMscIPuroR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctagacgcgtctggaacaatcaacctcgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggctgcagctccacgggcttcctggcgcgtggaccacgtactgggcgttcgggccacggatctgcgcagaccttgttagttggaMluIPfoISexAIAflIIIBssHIIBstAPIDraIIIPuroRWPRE1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcgacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgWPRE2,1202,1402,1602,1802,2002,2202,2402,260
    ccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgaPfoIPflMIWPRE2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcaacccgtgactgttaaggcaccacaacagccccttcgactgcaggaaaggtaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcNaeISacIINgoMIVWPRE2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctggaattaattctgcagtcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggtttgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcggaccttaattaagacgtcagctctggatctttttgtacctcgttagtgttcatcgttatgtcgtcgatggttacgactaacacggaccgatcttcgtgttctcctcctcctccacccaaaPstIWPRE2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaagaggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttctcccctgaccttcccgattaagtgagggttgcttctgttctagacgaaaaacgaacatgacccagagagaccaatctggtctagactcggaccctcKpnIAcc65IBsu36I3' LTR2,8202,8402,8602,8802,9002,9202,9402,960
    ctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcatcatcaagtacagtagaataataagtcaAflII3' LTR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    atttataacttgcaaagaaatgaatatcagagagtgagaggccttgacattgctagcgttttaccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtataaatattgaacgtttctttacttatagtctctcactctccggaactgtaacgatcgcaaaatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatBmtINheIPsiIlac promoterLacOM13-rev3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcSapIBspQI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgNspIPciIAflIIIColE1 origin3,5203,5403,5603,5803,6003,6203,6403,660
    gagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgaDrdIColE1 origin3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaggtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtcColE1 origin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaColE1 origin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttBspHIColE1 origin4,2204,2404,2604,2804,3004,3204,3404,360
    tctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgcAhdIW220HK218IL216SA214GI212EA210IQ208RN206RE204DM202TA200QS198GT196TY194IV192VI190RS188PK186GD184PG182LA180AI178IG176RAmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    agacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgcttctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgaAclIFspIS174GR172EG170AG168SK166DA164IF162WG160AP158LA156SR154LL152PG150AV148KD146AE144MW142DI140LQ138QR136SA134LT132LL130EG128TL126LK124RL122TT120AM118AAmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    acaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggtgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccPvuIV116PM114TT112DR110ED108NP106IA104EN102LE100PE98WR96DL94RT92VH90DG88MN86HL84FA82TL80EK78PG76GI74TT72LL70LN68AA66TN64DS62MT60IA58AmpR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcatgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaScaIAS56CL54ER52VT50MG48DT46LH44KE42TV40PS38YE36VL34DN32QS30YH28IR26RG24LQ22EQ20GA18DI16RS14LV12AG10CL8LV6KF4TS2MAmpR4,9204,9404,9604,9805,0005,0205,0405,060
    tggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtAclI5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcaacgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagttgSspIBspHISgrDI5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcaactggataactcaagctaaccaaaatcatcccaaacttcccaaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctagttgacctattgagttcgattggttttagtagggtttgaagggPsiISV40-PA terminator5,4405,4605,4805,5005,5205,5405,5605,5805,600
    accccataccctattaccactgccaattacctgtggtttcatttactctaaacctgtgattcctctgaattattttcattttaaagaaattgtatttgttaaatatgtactacaaacttagtagttggaagggctaattcactcccaaagaagacaagatatccttgatctgtggtggggtatgggataatggtgacggttaatggacaccaaagtaaatgagatttggacactaaggagacttaataaaagtaaaatttctttaacataaacaatttatacatgatgtttgaatcatcaaccttcccgattaagtgagggtttcttctgttctataggaactagacacc5,6205,6405,6605,6805,7005,7205,7405,760
    atctaccacacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgtagatggtgtgtgttccgatgaagggactaatcgtcttgatgtgtggtcccggtccccagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcggtctattccatcttctccggttatttcctctcttgtggtcgaacaatgtgggacactcggacgtacDraIII5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggatggatgacccggagagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactgctgatatcgagcttgctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactggcctacctactgggcctctctcttcacaatctcacctccaaactgtcggcggatcgtaaagtagtgcaccgggctctcgacgtaggcctcatgaagttcttgacgactatagctcgaacgatgttccctgaaaggcgacccctgaaaggtccctccgcaccggacccgccctgaccPmlIScaIBsaAIBspEI5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ggagtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgcctcaccgctcgggagtctaggacgtatattcgtcgacgaaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacAflII5' LTR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtaactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagagagctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatBplIBplIBssHIIPsi5' LTR6,3206,3406,3606,3806,4006,4206,4406,460
    cgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtccccctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgNruIGAGPsi6,4806,5006,5206,5406,5606,5806,6006,6206,640
    agttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgttgggagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttcGAG6,6606,6806,7006,7206,7406,7606,7806,8006,820
    atagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggttatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatatttcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccaFseIMfeINaeISapIBspQIEcoNINgoMIVGAG6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatcgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacagaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtaBbvCIPRE7,0207,0407,0607,0807,1007,1207,1407,160
    ctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttgacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaacgtggtgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaaBstAPIPREENV7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataaccttaatctatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatENV7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtggagggttggggctcccctgggctgtccgggcttccttatcttcttcttccacctctcKflIPpuMIEcoO109IENV7,5407,5607,5807,6007,6207,6407,6607,6807,700
    agagacagagacagatccattcgattagtgaacggatctcgacggtatcgcctttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttctctgtctctgtctaggtaagctaatcacttgcctagagctgccatagcggaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaagcccaaCPPTENV7,7207,7407,7607,7807,8007,8207,8407,860
    tattacagggacagcagagatccagataatgtccctgtcgtctctaggtc7,8807,8907,900
    • Plasmid
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    Editing disabled on read-only sequence.
    7900 bp5500600065007000750050010001500PuroRGAGtruncated GFP (filler)EGFP-N

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    7900