Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pRS138 Addgene
  • Sequence Map
    Editing disabled on read-only sequence.
    tttatcgataagcttgggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttaaatagctattcgaaccctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaClaIBspDICMV promoterCMV enhancer20406080100120140160
    ggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttaNdeIBsaAICspCISnaBICMV promoterCMV enhancerCMV promoter180200220240260280300320340
    gggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctccccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagCMV promoterCMV promoter360380400420440460480500520
    gtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgactctactagaggatctatttccggtgaattcccgagcgtgtcagggtgaccgtggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggctgagatgatctcctagataaaggccacttaagggctcgcacagtcccactggcaccactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctEcoRIBstEIIRegion to be replaced with BC fragmentCMV promotertruncated GFP (filler)BC-C1 (updag)EGFP-N540560580600620640660680700
    cggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatggccgctgcatttgccggtgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggactggatgccgcacgtcacgaagtcggcgatggggctggtgtacRegion to be replaced with BC fragmenttruncated GFP (filler)720740760780800820840860880
    aagctctagagtattaccatggtcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaggatccaattctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatcttcgagatctcataatggtaccagttttttttttttttttttttttttttttttcctaggttaagatggcccatcccctccgcgaaaagggttccgtcagacctcgtacgcgaaatcgtcggggcgacccgtgaaccgcgatgtgttcaccggagaccggagcgtgtgtaaggtgtagNspISphIXbaIBamHIPGK promoterpolyA(30)9009209409609801,0001,0201,0401,060
    caccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcactagtctcgtgcagatggacagcaccgctgagcaatggaaggtggccatccgcggttggccgaggcaagaaaccaccggggaagcgcggtggaagatgaggaggggatcagtccttcaagggggggcggggcgtcgagcgcagcacgtcctgcactgtttaccttcatcgtgcagagtgatcagagcacgtctacctgtcgtggcgactcgttaccttcAgeIBlpISpeIPGK promoter1,0801,1001,1201,1401,1601,1801,2001,2201,240
    cgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccggcccatccggaaaccccgtcgccggttatcgtcgaaacgaggaagcgaaagacccgagtctccgacccttccccacccaggcccccgcccgagtccccgcccgagtccccgccccgcccgcgggcttccaggaggcctccgggccgtaagacgtgcgaagttttcgcgtgcagacggcBspEIPpuMIEcoO109IPGK promoter1,2601,2801,3001,3201,3401,3601,3801,4001,420
    cgctgttctcctcttcctcatctccgggcctttcgacctgcagttaacgccgccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccgcgacaagaggagaaggagtagaggcccggaaagctggacgtcaattgcggcggtactggctcatgttcgggtgccacgcggagcggtgggcgctgctgcagggggcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctgggcctggcggHpaIPstISmaIXmaIBfuAIBsiWIBspMIPflFIRsrIITspMITth111IPuroRPGK promoter1,4401,4601,4801,5001,5201,5401,5601,5801,600
    acatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttctgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccacacccagcgcctgctgccgcggcgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagSacIIBstEIIPuroR1,6201,6401,6601,6801,7001,7201,7401,7601,780
    ccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctgggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgcaccaaggaccggtggcagccgcagagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacMscIPfoIBssHIIPuroR1,8001,8201,8401,8601,8801,9001,9201,940
    gagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctagacgcgtctggaacaatcaacctctggattacaaaatttgtgaaagattgactggtattctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggctgcagctccacgggcttcctggcgcgtggaccacgtactgggcgttcgggccacggatctgcgcagaccttgttagttggagacctaatgttttaaacactttctaactgaccataaMluISexAIAflIIIBstAPIDraIIIPuroRWPRE1,9601,9802,0002,0202,0402,0602,0802,1002,120
    cttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttggaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacWPRE2,1402,1602,1802,2002,2202,2402,2602,2802,300
    ctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagccccttcgactgPfoIPflMIWPRE2,3202,3402,3602,3802,4002,4202,4402,4602,480
    gtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggcccaggaaaggtaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggNaeISacIINgoMIVWPRE2,5002,5202,5402,5602,5802,6002,6202,6402,660
    gcctccccgcctggaattaattctgcagtcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagccggaggggcggaccttaattaagacgtcagctctggatctttttgtacctcgttagtgttcatcgttatgtcgtcgatggttacgactaacacggaccgatcttcgtgttctcctcctcctccacccaaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgKpnIPstIAcc65IBsu36IWPRE2,6802,7002,7202,7402,7602,7802,8002,8202,840
    tgtagatcttagccactttttaaaagaaaagaggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcctacatctagaatcggtgaaaaattttcttttctcccctgaccttcccgattaagtgagggttgcttctgttctagacgaaaaacgaacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaAflII3' LTR2,8602,8802,9002,9202,9402,9602,9803,0003,020
    tgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggccttgacattgctactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcatcatcaagtacagtagaataataagtcataaatattgaacgtttctttacttatagtctctcactctccggaactgtaacgaNheIPsiI3' LTR3,0403,0603,0803,1003,1203,1403,1603,1803,200
    agcgttttaccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgctcgcaaaatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgBmtIlac promoterLacOM13-rev3,2203,2403,2603,2803,3003,3203,3403,3603,380
    ccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatcggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaatagSapIBspQI3,4003,4203,4403,4603,4803,5003,5203,5403,560
    cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacgtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatgDrdINspIPciIAflIIIColE1 origin3,5803,6003,6203,6403,6603,6803,7003,720
    caggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccgtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggColE1 origin3,7403,7603,7803,8003,8203,8403,8603,8803,900
    ccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgColE1 origin3,9203,9403,9603,9804,0004,0204,0404,0604,080
    agtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgactcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgColE1 origin4,1004,1204,1404,1604,1804,2004,2204,2404,260
    gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagaBspHIW220HK218IL216SA214GI212EA210IQ208AmpRColE1 origin4,2804,3004,3204,3404,3604,3804,4004,4204,440
    gtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgccagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgAhdIRN206RE204DM202TA200QS198GT196TY194IV192VI190RS188PK186GD184PG182LA180AI178IG176RS174GR172EG170AG168SK166DA164IF162WG160AP158LA156SR154LL152PG150AAmpR4,4604,4804,5004,5204,5404,5604,5804,6004,620
    aactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgattgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactAclIFspIV148KD146AE144MW142DI140LQ138QR136SA134LT132LL130EG128TL126LK124RL122TT120AM118AV116PM114TT112DR110ED108NP106IA104EN102LE100PE98WR96DL94RT92VH90AmpR4,6404,6604,6804,7004,7204,7404,7604,7804,800
    tcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcPvuIScaIDG88MN86HL84FA82TL80EK78PG76GI74TT72LL70LN68AA66TN64DS62MT60IA58AS56CL54ER52VT50MG48DT46LH44KE42TV40PS38YE36VL34DN32QS30AmpR4,8204,8404,8604,8804,9004,9204,9404,9604,980
    aatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgacAclIYH28IR26RG24LQ22EQ20GA18DI16RS14LV12AG10CL8LV6KF4TS2MAmpR5,0005,0205,0405,0605,0805,1005,1205,1405,160
    atcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttSspIBspHI5,1805,2005,2205,2405,2605,2805,3005,3205,340
    tgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcaactttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagttgaaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccPsiISgrDISV40-PA terminator5,3605,3805,4005,4205,4405,4605,4805,500
    tttgtccaaactcatcaatgtatcttatcatgtctggatcaactggataactcaagctaaccaaaatcatcccaaacttcccaccccataccctattaccactgccaattacctgtggtttcatttactctaaacctgtgattcctctgaattattttcattttaaagaaattgtattaaacaggtttgagtagttacatagaatagtacagacctagttgacctattgagttcgattggttttagtagggtttgaagggtggggtatgggataatggtgacggttaatggacaccaaagtaaatgagatttggacactaaggagacttaataaaagtaaaatttctttaacataaSV40-PA terminator5,5205,5405,5605,5805,6005,6205,6405,6605,680
    tgttaaatatgtactacaaacttagtagttggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaacaatttatacatgatgtttgaatcatcaaccttcccgattaagtgagggtttcttctgttctataggaactagacacctagatggtgtgtgttccgatgaagggactaatcgtcttgatgtgtggtcccggtccccagtctataggtgactggaaacctaccacgatgttcgatcat5,7005,7205,7405,7605,7805,8005,8205,8405,860
    ccagttgagccagataaggtagaagaggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactggtcaactcggtctattccatcttctccggttatttcctctcttgtggtcgaacaatgtgggacactcggacgtaccctacctactgggcctctctcttcacaatctcacctccaaactgtcggcggatcgtaaagtagtgcaccgggctctcgacgtaggcctcatgaagttcttgaPmlIScaIBsaAIBspEIDraIII5,8805,9005,9205,9405,9605,9806,0006,0206,040
    gctgatatcgagcttgctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactggggagtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagcgactatagctcgaacgatgttccctgaaaggcgacccctgaaaggtccctccgcaccggacccgccctgacccctcaccgctcgggagtctaggacgtatattcgtcgacgaaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatc5' LTR6,0606,0806,1006,1206,1406,1606,1806,2006,220
    ggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagBplIAflII5' LTR6,2406,2606,2806,3006,3206,3406,3606,3806,400
    tctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagagagctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtcBplINruIBssHIIPsiGAG6,4206,4406,4606,4806,5006,5206,5406,5606,580
    ggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagccccctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgGAG6,6006,6206,6406,6606,6806,7006,7206,7406,760
    aaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatatattgggagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatatFseIMfeINaeISapIBspQIEcoNINgoMIVGAG6,7806,8006,8206,8406,8606,8806,9006,9206,940
    aagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtcttcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacagPRE6,9606,9807,0007,0207,0407,0607,0807,1007,120
    tggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaacgtggBbvCIBstAPIPRE7,1407,1607,1807,2007,2207,2407,2607,280
    actgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagatatgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataaccttaatctatENVPRE7,3007,3207,3407,3607,3807,4007,4207,4407,460
    aatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtggagggttggggENV7,4807,5007,5207,5407,5607,5807,6007,6207,640
    gaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgcctttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaactcccctgggctgtccgggcttccttatcttcttcttccacctctctctctgtctctgtctaggtaagctaatcacttgcctagagctgccatagcggaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtttgattKflIPpuMIEcoO109IENVCPPT7,6607,6807,7007,7207,7407,7607,7807,8007,820
    agaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtcttaatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtccctgtcgtctctaggtcCPPT7,8407,8607,8807,900
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7900 bp5500600065007000750050010001500PuroRGAGtruncated GFP (filler)EGFP-NCMV promoter,CMV enhancer,BspDI,ClaI,NdeI,BsaAI,SnaBI,CspCICMV promoter,EcoR1,BC-C1 (updag),EcoRI,BstEIIRegion to be replaced with BC fragment,XbaIpolyA(30),MCS,BamHI,NspI,SphIPGK promoter,AgeI,SpeIBlpIEcoO109I,PpuMI,BspEI,PstI,BfuAI,BspMI,HpaIPflFI,Tth111I,TspMI,XmaI,SmaI,BsiWI,RsrII,BstEIISacIIMscIBssHIIPfoIDraIIIBsaAI,PmlI,BspEI,ScaI5' LTR,AflIIBplIPsi,BplI,BssHIINruIBspQI,SapINgoMIV,NaeI,FseI,EcoNI,MfeIBbvCIPRE,BstAPIENV,CPPT,EcoO109I,KflI,PpuMI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    7900