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  • 0 feature results
B880_start_Cas9_ST_stop > pJ2
  • Sequence Map
    Editing disabled on read-only sequence.
    AgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatTctagaaaaagggagacggtttttaatacccctgtagtacttcggggaactcgtagactgaagaccgattatttcctttaaataaaagtaacgttatcacacaaccttaaaaaacacagagagtgagccttcctgtataccctcccgtttagtaaattttgtagtcttactcataBglIIrBG PA20406080100120140160
    ttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttcttaaccaaatctcaaaccgttgtatacgggtatacgaccgacggtacttgtttccaaccgatatttctccagtagtcatatactttgtcgggggacgacaggtaaggaataaggtatcttttcggaactgaactccaatctaaaaaaaatataaaacaaaacacaataaaaaaagaarBG PA180200220240260280300320340
    taacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgcagcccaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaaattgtagggattttaaaaggaatgtacaaaatgatcggtctaaaaaggaggagaggactgatgagggtcagtatcgacagggagaagagaatacctctagggagctggacgtcgggttcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttPciIAflIIIHindIIIrBG PAM13-revLacO360380400420440460480500520
    ttccacacaacatacgagccggaagcataaagtgtaaagcctgggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcggatccgcatctcaattagtcagcaaccatagtcccgcccctaactccgcaaggtgtgttgtatgctcggccttcgtatttcacatttcggacccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgcctaggcgtagagttaatcagtcgttggtatcagggcggggattgaggcgBamHIBsaBIlac540560580600620640660680700
    ccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctaacttgtttattgcagctggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgattgaacaaataacgtcgaBplIBplISfiIStuIAvrIISV40 ORISV40 pA720740760780800820840860
    tataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccgctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctaggcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagBsmIPsiIBamHISV40 pA8809009209409609801,0001,0201,040
    ttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccPciISapIBspQIAflIIIColE...gin1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggDrdIColE1 origin1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactgaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgacApaLIColE1 origin1,4201,4401,4601,4801,5001,5201,5401,560
    gcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttColE1 origin1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaColE1 origin1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtAhdIAmpR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tctggccccagtgctgcaatgataccgcgGCTTCCacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaagaccggggtcacgacgttactatggcgcCGAAGGtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaatBpmIAmpR2,1202,1402,1602,1802,2002,2202,2402,260
    atagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagtttatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaAclIFspIPvuIAmpR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtBcgIAmpR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    catagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaagtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgtttAclIApaLIAmpR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    atgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgcctacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggSspIAmp prom2,8202,8402,8602,8802,9002,9202,9402,960
    acctgggtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagttggacccagctgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcaAccISalISpeIHincIICAG__CMV enhancer2,9803,0003,0203,0403,0603,0803,1003,1203,140
    aacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctactttgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaCAG__CMV enhancer3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggccccccccaggcgaccgtcatgtagatgcataatcagtagcgataatggtaccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccccggggggggtccgcBtgZIBtgZISnaBICAG__AG PromoterCMV enhancer3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gggcggggcggggcgaggggcggggcggggcgaggcggaaaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgttgcgcgctgccttccccccgtgcccccgccccgccccgctccccgccccgccccgctccgcctttccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcaacgcgcgacggaaggggggcacggCAG__AG Promoter3,5203,5403,5603,5803,6003,6203,6403,660
    ccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctggcgaggcggcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgaacaaagaaaagacaccgacgcactttcggaactccccgaggccctcccgggaAfeICAG__AG PromoterCAG INTRONCAGGS INTRON REGION3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggaacacgcccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgaggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgtcacacgcgctcccctcgcgccggcccccgccacggggcgccacgccccAfeIEagICAG__AG PromoterCAG INTRONCAGGS INTRON REGION3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtccccgacgctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgcagccagcccgacgttgggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcatgccccgcaccgcgccccgagcggcaBsiWIAG PromoterCAG INTRONCAGGS INTRON REGIONCAG__4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcccggcccgccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagcccccttccccgcgccgccgggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgaaggMreIPaqCISgrAIAG PromoterCAG INTRONCAGGS INTRON REGION4,2204,2404,2604,2804,3004,3204,3404,360
    tttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcgggggaaacagggtttagacacgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgcgccccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagagggagaggtcggagccccgacaggcgcccccBpmIKasIKasINarINarISfoISfoIPluTIPluTIAG PromoterCAG INTRONCAGGS INTRON REGION4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaaGTACCGAGCTCagctgccgacggaagccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcgaggacccgttgcacgaccaataacacgacagagtagtaaaaccgtttcttCATGGCTCGAGtcSacIXbaIEco53kIAG PromoterCAG INTRONCAGGS INTRON REGION4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gtacctacagccgccaccATGggagcaGCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCcatggatgtcggcggtggTACcctcgtCGGGGTTTCTTCTTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGTCGGCTGTTCTTCATGTCGTAGCCGGACCTGTAGCCGTGGTTGAGACACCCGACCCGGCACTAGTGGCTGCTCATGTTCCACGGGTCGTTCTTTAAGTTCCACGKpnIBstXIAcc65ISpCas9(wt)SV40 NLS4,7404,7604,7804,8004,8204,8404,8604,8804,900
    TGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTACCCGTTGTGGCTGGCCGTGTCGTAGTTCTTCTTGGACTAGCCTCGGGACGACAAGCTGTCGCCGCTTTGTCGGCTCCGGTGGGCCGACTTCTCTTGGCGGTCTTCTTCTATGTGGTCTGCCTTCTTGGCCTAGACGATAGACGTTCTCTAGAAGTCGTTGCTCTACCGGTTCCABglIISpCas9(wt)4,9204,9404,9604,9805,0005,0205,0405,060
    GGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCCTGCTGTCGAAGAAGGTGTCTGACCTTCTCAGGAAGGACCACCTTCTCCTATTCTTCGTGCTCGCCGTGGGGTAGAAGCCGTTGTAGCACCTGCTCCACCGGATGGTGCTCTTCATGGGGTGGTAGATGGTGGACTCTTTCTTTGACCACCTGTCGTGGCTGTTCCGGCTGGACSpCas9(wt)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    CGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGGCCGACTAGATAGACCGGGACCGGGTGTACTAGTTCAAGGCCCCGGTGAAGGACTAGCTCCCGCTGGACTTGGGGCTGTTGTCGCTGCACCTGTTCGACAAGTAGGTCGACCACGTCTGGATGTTGGTCGACAAGCTCCTTTTGGGGTAGTTGCGGTCGCCGCACCTGCGGTTCCBmgBIBstXIEcoNISpCas9(wt)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGTAGGACAGACGGTCTGACTCGTTCTCGTCTGCCGACCTTTTAGACTAGCGGGTCGACGGGCCGCTCTTCTTCTTACCGGACAAGCCTTTGGACTAACGGGACTCGGACCCGGACTGGGGGTTGAAGTTCTCGTTGAAGCTGGACCGGCTCCTACGGTTTGACGTCGACTCGTTEcoNISpCas9(wt)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACCCTGTGGATGCTGCTGCTGGACCTGTTGGACGACCGGGTCTAGCCGCTGGTCATGCGGCTGGACAAAGACCGGCGGTTCTTGGACAGGCTGCGGTAGGACGACTCGCTGTAGGACTCTCACTTGTGGCTCTAGTGGTTCCGGGGGGACTCGCGGAGATACTAGTTCTCTATGCTGSpCas9(wt)5,6205,6405,6605,6805,7005,7205,7405,760
    GAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGCTCGTGGTGGTCCTGGACTGGGACGACTTTCGAGAGCACGCCGTCGTCGACGGACTCTTCATGTTTCTCTAAAAGAAGCTGGTCTCGTTCTTGCCGATGCGGCCGATGTAACTGCCGCCTCGGTCGGTCCTTCTCAAGATGTTCAAGTAGTTCGGGTAGGACCTTTTCTACCTGCSpCas9(wt)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    GCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCGTGGCTCCTTGACGAGCACTTCGACTTGTCTCTCCTGGACGACGCCTTCGTCGCCTGGAAGCTGTTGCCGTCGTAGGGGGTGGTCTAGGTGGACCCTCTCGACGTGCGGTAAGACGCCGCCGTCCTTCTAAAAATGGGTAAGGACTTCCTGTTGGCCCTTTTCTAGCTCTTCTASpCas9(wt)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    CCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACGGACTGGAAGGCGTAGGGGATGATGCACCCGGGAGACCGGTCCCCTTTGTCGTCTAAGCGGACCTACTGGTCTTTCTCGCTCCTTTGGTAGTGGGGGACCTTGAAGCTCCTTCACCACCTGTTCCCGCGAAGGCGGGTCTCGAAGTAGCTCGCCTACTGGTTGAAGCTATTCTTGSpCas9(wt)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    CTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGAGACGGGTTGCTCTTCCACGACGGGTTCGTGTCGGACGACATGCTCATGAAGTGGCACATATTGCTCGACTGGTTTCACTTTATGCACTGGCTCCCTTACTCTTTCGGGCGGAAGGACTCGCCGCTCGTCTTTTTCCGGTAGCACCTGGACGACAAGTTCTGGTTGGCCTTTCACTBarISpCas9(wt)6,3206,3406,3606,3806,4006,4206,4406,460
    CCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGAGGCACTTCGTCGACTTTCTCCTGATGAAGTTCTTTTAGCTCACGAAGCTGAGGCACCTTTAGAGGCCGCACCTTCTAGCCAAGTTGCGGAGGGACCCGTGTATGGTGCTAGACGACTTTTAATAGTTCCTGTTCCTGAAGGACCTGTTACTCCTTTTGCTCCTGTAAGACCTTCTPasISpCas9(wt)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    TATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCATAGCACGACTGGGACTGTGACAAACTCCTGTCTCTCTACTAGCTCCTTGCCGACTTTTGGATACGGGTGGACAAGCTGCTGTTTCACTACTTCGTCGACTTCGCCGCCTCTATGTGGCCGACCCCGTCCGACTCGGCCTTCGACTAGTTGCCGTAGGCCCTGTTCGTCAGGCCGAhdIEcoRVSpCas9(wt)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    AAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGATTCTGTTAGGACCTAAAGGACTTCAGGCTGCCGAAGCGGTTGTCTTTGAAGTACGTCGACTAGGTGCTGCTGTCGGACTGGAAATTTCTCCTGTAGGTCTTTCGGGTCCACAGGCCGGTCCCGCTATCGGACGTGCTCGTGTAACGGTTAGACCGGCCGTCGGGGCGGTAATTCTBcgISpCas9(wt)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    AGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGTCCCGTAGGACGTCTGTCACTTCCACCACCTGCTCGAGCACTTTCACTACCCGGCCGTGTTCGGGCTCTTGTAGCACTAGCTTTACCGGTCTCTCTTGGTCTGGTGGGTCTTCCCTGTCTTCTTGTCGGCGCTCTCTTACTTCGCCTAGCTTCTCCCGTAGTTTCTCGACCCGTCSacIEco53kISpCas9(wt)7,0207,0407,0607,0807,1007,1207,1407,160
    CCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGAAGGACGACTCCGGTCTAGGACTTTCTTGTGGGGCACCTTTTGTGGGTCGACGTCTTGCTCTTCGACATGGACATGATGGACGTCTTACCCGCCCTATACATGCACCTGGTCCTTGACCTGTAGTTGGCCGACAGGCTGATGCTACACCTGGTATAGCACGGAGTCTCGAAAGACTTCCTGCTGAGGDraIIISpCas9(wt)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGTAGCTGTTGTTCCACGACTGGTCTTCGCTGTTCTTGGCCCCGTTCTCGCTGTTGCACGGGAGGCTTCTCCAGCACTTCTTCTACTTCTTGATGACCGCCGTCGACGACTTGCGGTTCGACTAATGGGTCTCTTTCAAGCTGTTAGACTGGTTCCGGCTCTCTCCGCCGGACTCGCSpCas9(wt)7,3607,3807,4007,4207,4407,4607,4807,5007,520
    AACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAATTGACCTATTCCGGCCGAAGTAGTTCTCTGTCGACCACCTTTGGGCCGTCTAGTGTTTCGTGCACCGTGTCTAGGACCTGAGGGCCTACTTGTGATTCATGCTGCTCTTACTGTTCGACTAGGCCCTTCACTTTCACTAGTGGGACTTCAGGTTCGACCACAGGCTAAAGGCCTTPmlIBspEICspCISpCas9(wt)7,5407,5607,5807,6007,6207,6407,6607,6807,700
    GGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCCTAAAGGTCAAAATGTTTCACGCGCTCTAGTTGTTGATGGTGGTGCGGGTGCTGCGGATGGACTTGCGGCAGCACCCTTGGCGGGACTAGTTTTTCATGGGATTCGACCTTTCGCTCAAGCACATGCCGCTGATGTTCCACATGCTGCACGCCTTCTACTAGCGGTTCTCGCTCBmgBISpCas9(wt)7,7207,7407,7607,7807,8007,8207,8407,860
    CAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAGTCCTTTAGCCGTTCCGATGGCGGTTCATGAAGAAGATGTCGTTGTAGTACTTGAAAAAGTTCTGGCTCTAATGGGACCGGTTGCCGCTCTAGGCCTTCGCCGGAGACTAGCTCTGTTTGCCGCTTTGGCCCCTCTAGCACACCCTATTCCCGGCCCTAAAACGGTGGCACGCCTBspEISpCas9(wt)7,8807,9007,9207,9407,9607,9808,0008,0208,040
    AAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTTTCACGACTCGTACGGGGTTCACTTATAGCACTTTTTCTGGCTCCACGTCTGTCCGCCGAAGTCGTTTCTCAGATAGGACGGGTTCTCCTTGTCGCTATTCGACTAGCGGTCTTTCTTCCTGACCCTGGGATTCTTCATGCCGCCGAAGCTGTCGGGGTGGCACCGGATAAGACABarIKflISphISpCas9(wt)8,0608,0808,1008,1208,1408,1608,1808,2008,220
    GCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACCGACCACCACCGGTTTCACCTTTTCCCGTTCAGGTTCTTTGACTTCTCACACTTTCTCGACGACCCCTAGTGGTAGTACCTTTCTTCGTCGAAGCTCTTCTTAGGGTAGCTGAAAGACCTTCGGTTCCCGATGTTTCTTCACTTTTTCCTGGACTAGTAGTTCGACGGATTCATGXcmIBsaBISpCas9(wt)8,2408,2608,2808,3008,3208,3408,3608,3808,400
    TCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAGGGACAAGCTCGACCTTTTGCCGGCCTTCTCTTACGACCGGAGACGGCCGCTTGACGTCTTCCCTTTGCTTGACCGGGACGGGAGGTTTATACACTTGAAGGACATGGACCGGTCGGTGATACTCTTCGACTTCCCGAGGGGGCTCCTATTACTCGTCTTTGTCGACAAACACCBsmIEagISpCas9(wt)8,4208,4408,4608,4808,5008,5208,5408,560
    AACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTTTGTCGTGTTCGTGATGGACCTGCTCTAGTAGCTCGTCTAGTCGCTCAAGAGGTTCTCTCACTAGGACCGGCTGCGATTAGACCTGTTTCACGACAGGCGGATGTTGTTCGTGGCCCTATTCGGGTAGTCTCTCGTCCGGCTCTTATAGTAGGTGGACAAATGGGACTGGTTAGASpCas9(wt)8,5808,6008,6208,6408,6608,6808,7008,7208,740
    GGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGCCCTCGGGGACGGCGGAAGTTCATGAAACTGTGGTGGTAGCTGGCCTTCTCCATGTGGTCGTGGTTTCTCCACGACCTGCGGTGGGACTAGGTGGTCTCGTAGTGGCCGGACATGCTCTGTGCCTAGCTGGACAGAGTCGACCCTCCGCTGTTTTCCGGCCGCCGGTGCTTTTTCXcmISpCas9(wt)8,7608,7808,8008,8208,8408,8608,8808,9008,920
    GCCGGCCAGGCAAAAAAGAAAAAGgaattcGGCAGTGGAggagccAGCACCTGAgaggattgcttCGGCCGGTCCGTTTTTTCTTTTTCcttaagCCGTCACCTcctcggTCGTGGACTctcctaacgaaFseIEcoRI8,9408,9608,980
    • Plasmid
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    • Assembly Parameters
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    Editing disabled on read-only sequence.
    8990 bp65007000750080008500500100015002000SpCas9(wt)AmpRpolyA (putative)

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    8990