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EW492 CMV-TO-UTRN-14ZF(N61K,H88F, I91E, K120F)-realdeImmLink-NZF(FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMV20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMV180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaNcoICspCISnaBICMV360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcBsaBIBsaBISgrDICMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgCACGAGTGTGCCCGATGTGGGAAGAATTTCTCTTGGCACTCAGACCTCATCTTGCACGAGCAAATCCACACAGGAGAGCGACCTTTCAAGTGTTACAAGTGTGGACGTGGATTttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacGTGCTCACACGGGCTACACCCTTCTTAAAGAGAACCGTGAGTCTGGAGTAGAACGTGCTCGTTTAGGTGTGTCCTCTCGCTGGAAAGTTCACAATGTTCACACCTGCACCTAABbsIPmeIXmnIAflIIBmgBIHindIIIM1HE2CA4RC6GK8NF10SW12HS14DL16IL18HE20QI22HT24GE26RP28FK30CY32KC34GR36GF38763-1428UTRN Promoter Pos(-14)-Binding ZF (Q5JNZ3 finger 1-Q6... 21-Q8NCA9 finger 4-Q8NCA9 finger 4-P49711 finger 1)720740760780800820840860
    CGCAGAGCACGGGACACTCAATCGACACCTTAGAACTAAGGGCGGATGTACTGGGGAGCGGCCTTACAAGTGTGAGGAGTGTGGAAGAGCTTTCTCCCAAGCTAGCCATCTCCAAGACCACCAAAGGCTTCACACTGGGGAGAGACCCTTCCAATGTgagGAGTGTGGAAAGTCCGCGTCTCGTGCCCTGTGAGTTAGCTGTGGAATCTTGATTCCCGCCTACATGACCCCTCGCCGGAATGTTCACACTCCTCACACCTTCTCGAAAGAGGGTTCGATCGGTAGAGGTTCTGGTGGTTTCCGAAGTGTGACCCCTCTCTGGGAAGGTTACActcCTCACACCTTTCAGGBmtINheISapIBspQIAE40HG42TL44NR46HL48RT50KG52GC54TG56ER58PY60KC62EE64CG66RA68FS70QA72SH74LQ76DH78QR80LH82TG84ER86PF88QC90EE92CG94KS96763-1428UTRN Promoter Pos(-14)-Binding ZF (Q5JNZ3 finger 1-Q66K89 finger 9-Q9NYT6 finger 19-O15535 finger 5-Q13398 finger 21-Q8NCA9 finger 4-Q8NCA9 finger 4-P49711 finger 1)8809009209409609801,0001,0201,040
    TTCAATAGACACTGTAATCTTATAAGGCACCAAAAGATACACACGGGTGAGCGGCCATTCGAGTGTTCTTTTTGTGGGAAGGCTTGTACTAGAAGGTGTAATTTGATACAACACCAAAAGGTCCACACTGGTGAGCGTCCTTTCGCTTGTAGGTTCTGTGCTAAGCCTTTCAGACAAGTTATCTGTGACATTAGAATATTCCGTGGTTTTCTATGTGTGCCCACTCGCCGGTAAGCTCACAAGAAAAACACCCTTCCGAACATGATCTTCCACATTAAACTATGTTGTGGTTTTCCAGGTGTGACCACTCGCAGGAAAGCGAACATCCAAGACACGATTCGGAAAGTCTGBlpIPsiIFN98RH100CN102LI104RH106QK108IH110TG112ER114PF116EC118SF120CG122KA124CT126RR128CN130LI132QH134QK136VH138TG140ER142PF144AC146RF148CA150KP152FR154763-1428UTRN Promoter Pos(-14)-Binding ZF (Q5JNZ3 finger 1-Q66K89 finger 9-Q9NYT6 finger 19-O15535 finger 5-Q13398 finger 21-Q8NCA9 finger 4-Q8NCA9 finger 4-P49711 finger 1)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GTTCTTCAGACATGCGAGACCACGAGAGAGTCCACACTGGGGAGAGACCATTCGCATGTCGGTTCTGTGCCAAGCCTTTCAGACGATCTTCCGACATGCGGGACCACGAGAGGGTTCACACAGGGGAGCGTCCCTTCCAATGTGAGCTCTGTTCATACACTTGTCCCAGAAGGTCCAAGAAGTCTGTACGCTCTGGTGCTCTCTCAGGTGTGACCCCTCTCTGGTAAGCGTACAGCCAAGACACGGTTCGGAAAGTCTGCTAGAAGGCTGTACGCCCTGGTGCTCTCCCAAGTGTGTCCCCTCGCAGGGAAGGTTACACTCGAGACAAGTATGTGAACAGGGTCTTCCAGRS156SD158MR160DH162ER164VH166TG168ER170PF172AC174RF176CA178KP180FR182RS184SD186MR188DH190ER192VH194TG196ER198PF200QC202EL204CS206YT208CP210RR212S763-1428UTRN Promoter Pos(-14)-Binding ZF (Q5JNZ3 finger 1-Q66K89 finger 9-Q9NYT6 finger 19-O15535 finger 5-Q13398 finger 21-Q8NCA9 finger 4-Q8NCA9 finger 4-P49711 finger 1)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AAATTTAGACAGACACATGAAGTCCCACgactggtcattaggcatgggcggatctGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCTGGTGAACCAGTTTAAATCTGTCTGTGTACTTCAGGGTGctgaccagtaatccgtacccgcctagaCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCGACCACTTGGTCAvaIBlpIBsgIPasIBsoBIBstXIDW2SL4GM6GG8SE10GQ12SD14ER16AL18LD20QL22HT24LL26SN28TD30AT32GL34EE36ID38RA40LG42IP44EL46VN48Q1429-1911N214LD216RH218MK220SH222763-1428UTRN Promot... finger 1)1,4201,4401,4601,4801,5001,5201,5401,560
    GGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGACCTGTTCCTGCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCTAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCTGGACAAGGACGPasIBmgBIEcoNIEcoRIPflMIPpuMIG50QA52LE54PK56QD58GG60WG62SM64AE66EF68VT70LK72DV74GM76DF78TL80GD82WE84QL86GL88EQ90GD92TF94WD96TA98LD100NC102QD104LF106L1429-19111,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGACACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCtaggccgctcgaggACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGatccggcgagctccAvaIPciITliIXhoIBsoBIBstXIPspXIPaeR7I*1L108DP110PG112GG114DG116LG118LG120HE122KF124PS126DL128DL130DM132FN134GS136LE138CD140ME142SI144IR146SE148LM150DA152DG154LD156FN158FD160S1429-19111,7601,7801,8001,8201,8401,8601,8801,9001,920
    agggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactccctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggApaIBclIPmeIPspOMIBGH polyadenylation sequencec-myc epitopePolyhi...e tag1,9401,9601,9802,0002,0202,0402,0602,0802,100
    actgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccBbsIClaISphIBspDIBGH polyadenylation sequenceBGH pA2,1202,1402,1602,1802,2002,2202,2402,260
    tgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgcacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcgBGH pA2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccgtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggMscINaeIXbaINgoMIVFRT2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttcBarIBsaXIPflFIPshAITth111IHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    atcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccgPstIBfuAIBspMIEcoRIDraIIIHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    catggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtcNcoINdeIPvuIAsiSIPflFIRsrIITth111IHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctcgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagaDraIIIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacAjuISacIIHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggScaIHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    gccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattttcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaNaeIPfoIPsiINgoMIVSV40 pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacattBsmIBstZ17ISV40 pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    agcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgSapIBsaXIBspQI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgcPciIpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagatgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaapUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttaBspHIpUC ori4,9204,9404,9604,9805,0005,0205,0405,060
    ctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctAhdIBsrDIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctactgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatAclIFspIBsrDIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    caggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgPvuIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcatttcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaBcgIScaIAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    ggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgAclIXmnIAmp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctccccctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggSspIBspHISgrDIbla promoterAmp (bla)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaMfeIBpu10I6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttgcgctgcttcgtacgggccagatatacgcaacgcgacgaagcatgcccggtctatatgcgMluIBsiWI6,320
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6331 bp450050005500600050010001500K121FTetO760-7621429-1911763-1428EWo229.3 (DNA)EWo211 (DNA)FrtF (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)

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    6331