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pAAV-cfos-hChR2 (H134R)-EYFP-PEST-no WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaagctttcctttaggaacagggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcgaaaggaaatccttgtcMluISbfIAflIIIHindIIIL-ITRFos Tru...ron 1)20406080100120140160
    aggcttcgagcctttaaggctgcgtacttgcttctcctaataccagagactcaaaaaaaaaaaaaaagttccagattgctggacaatgacccgggtctcatcccttgaccctgggaaccgggtccacattgaatcaggtgcgaatgttcgctcgccttctctgcctttcccgccttccgaagctcggaaattccgacgcatgaacgaagaggattatggtctctgagtttttttttttttttcaaggtctaacgacctgttactgggcccagagtagggaactgggacccttggcccaggtgtaacttagtccacgcttacaagcgagcggaagagacggaaagggcggaPasIPshAIFos Truncation (after Intron 1)180200220240260280300320340
    cccctcccccggccgcggccccggttccccccctgcgctgcaccctcagagttggctgcagccggcgagctgttcccgtcaatccctccctcctttacacaggatgtccatattaggacatctgcgtcagcaggtttccacggccggtccctgttgttctgggggggggaccatcggggagggggccggcgccggggccaagggggggacgcgacgtgggagtctcaaccgacgtcggccgctcgacaagggcagttagggagggaggaaatgtgtcctacaggtataatcctgtagacgcagtcgtccaaaggtgccggccagggacaacaagaccccccccctggtagSacIIFos Truncation (after Intron 1)360380400420440460480500520
    tccgaaatcctacacgcggaaggtctaggagaccccctaagatcccaaatgtgaacactcataggtgaaagatgtatgccaagacgggggttgaaagcctggggcgtagagttgacgacagagcgcccgcagagggccttggggcgcgcttccccccccttccagttccgcccagaggctttaggatgtgcgccttccagatcctctgggggattctagggtttacacttgtgagtatccactttctacatacggttctgcccccaactttcggaccccgcatctcaactgctgtctcgcgggcgtctcccggaaccccgcgcgaaggggggggaaggtcaaggcgggtcHincIIFos Truncation (after Intron 1)540560580600620640660680700
    tgacgtaggaagtccatccattcacagcgcttctataaaggcgccagctgaggcgcctactactccaaccgcgactgcagcgagcaactgagaagactggatagagccggcggttccgcgaacgagcagtgaccgcgctcccacccagctctgctctgcagctcccaccagtgtcactgcatccttcaggtaggtaagtgtcgcgaagatatttccgcggtcgactccgcggatgatgaggttggcgctgacgtcgctcgttgactcttctgacctatctcggccgccaaggcgcttgctcgtcactggcgcgagggtgggtcgagacgagacgtcgagggtggtcacagAccIAfeIFos Truncation (after Intron 1)720740760780800820840860
    tacccctggaccccttgccgggctttccccaaacttcgaccatgatgttctcgggtttcaacgccgactacgaggcgtcatcctcccgctgcagtagcgcctccccggccggggacagcctttcctactaccattccccagccgactccttctccagcatgggctctcctgtcaaatggggacctggggaacggcccgaaaggggtttgaagctggtactacaagagcccaaagttgcggctgatgctccgcagtaggagggcgacgtcatcgcggaggggccggcccctgtcggaaaggatgatggtaaggggtcggctgaggaagaggtcgtacccgagaggacagttHincIIFos Truncation (after Intron 1)8809009209409609801,0001,0201,040
    cacacaggtgagtttggctttgtgtagccgccaggtccgcgctgagggtcgccgtggaggagacactggggtgtgactcgcaggggcgggggggtcttcctttttcgctctggagggagactggcgcggtcagagcagccttagcctgggaacccaggacttgtctgagcgcgtggtgtgtccactcaaaccgaaacacatcggcggtccaggcgcgactcccagcggcacctcctctgtgaccccacactgagcgtccccgcccccccagaaggaaaaagcgagacctccctctgaccgcgccagtctcgtcggaatcggacccttgggtcctgaacagactcgcgcacFos Truncation (after Intron 1)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cacacttgtcatagtaagacttagtgaccccttcccgcgcggcaggtttattctgagtggcctgcctgcattcttctctcggccgacttgtttctgagatcagccggggccaacaagtctcgagcaaagagtcgctaactagagtttgggaggcggcaaaccgcggcaatcccccgtgtgaacagtatcattctgaatcactggggaagggcgcgccgtccaaataagactcaccggacggacgtaagaagagagccggctgaacaaagactctagtcggccccggttgttcagagctcgtttctcagcgattgatctcaaaccctccgccgtttggcgccgttagggggBsmISacIIFos Truncation (after Intron 1)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ctcccggggcagcctggagcagggaggagggaggagggaggagggtgctgcgggcgggtgtgtaaggcagtttcattgataaaaagcgagttcattctggagactccggagcagcgcctgcgtcagcgcagacgtcagggatatttataacaaaccccctttcgagcgagtgatggagggccccgtcggacctcgtccctcctccctcctccctcctcccacgacgcccgcccacacattccgtcaaagtaactatttttcgctcaagtaagacctctgaggcctcgtcgcggacgcagtcgcgtctgcagtccctataaatattgtttgggggaaagctcgctcactacPsiIZraIAatIIBspEIEcoNIFos Truncation (after Intron 1)1,4201,4401,4601,4801,5001,5201,5401,560
    ccgaagggataacgggaacgcagcagtaggatggaggagaaaggctgcgctgcggaattcaagggaggatattgggagagcttttatctccgatgaggtgcatacaggaagacataagcagtctctgaccggaatgcttctctctccctgcttcatgcgacactagggccacttgggcttccctattgcccttgcgtcgtcatcctacctcctctttccgacgcgacgccttaagttccctcctataaccctctcgaaaatagaggctactccacgtatgtccttctgtattcgtcagagactggccttacgaagagagagggacgaagtacgctgtgatcccggtgaacBsmIEcoRIFos Truncation (after Intron 1)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctccacctgtgtctggaacctcctcgctcacctccgctttcctctttttgttttgtttcagtaaaccggtgccaccatggactatggcggcgctttgtctgccgtcggacgcgaacttttgttcgttactaatcctgtggtggtgaacgggtccgtcctggtccctgaggatcaagaggtggacacagaccttggaggagcgagtggaggcgaaaggagaaaaacaaaacaaagtcatttggccacggtggtacctgataccgccgcgaaacagacggcagcctgcgcttgaaaacaagcaatgattaggacaccaccacttgcccaggcaggaccagggactcctagttAgeIBsu36IhChR2 (H134R)Fos Truncation (after Intron 1)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tgttactgtgccggatggattgaatctcgcggcacgaacggcgctcagaccgcgtcaaatgtcctgcagtggcttgcagcaggattcagcattttgctgctgatgttctatgcctaccaaacctggaaatctacatgcggctgggaggagatctatgtgtgcgccattgaaatggacaatgacacggcctacctaacttagagcgccgtgcttgccgcgagtctggcgcagtttacaggacgtcaccgaacgtcgtcctaagtcgtaaaacgacgactacaagatacggatggtttggacctttagatgtacgccgaccctcctctagatacacacgcggtaactttaccBglIIPflFITth111IhChR2 (H134R)1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ttaaggtgattctcgagttcttttttgagtttaagaatccctctatgctctaccttgccacaggacaccgggtgcagtggctgcgctatgcagagtggctgctcacttgtcctgtcatccttatccgcctgagcaacctcaccggcctgagcaacgactacagcaggagaaccataattccactaagagctcaagaaaaaactcaaattcttagggagatacgagatggaacggtgtcctgtggcccacgtcaccgacgcgatacgtctcaccgacgagtgaacaggacagtaggaataggcggactcgttggagtggccggactcgttgctgatgtcgtcctcttggtaDraIIIhChR2 (H134R)2,1202,1402,1602,1802,2002,2202,2402,260
    gggactccttgtctcagacatcgggactatcgtgtggggggctaccagcgccatggcaaccggctatgttaaagtcatcttcttttgtcttggattgtgctatggcgcgaacacattttttcacgccgccaaagcatatatcgagggttatcatactgtgccaaagggtcggtgcccctgaggaacagagtctgtagccctgatagcacaccccccgatggtcgcggtaccgttggccgatacaatttcagtagaagaaaacagaacctaacacgataccgcgcttgtgtaaaaaagtgcggcggtttcgtatatagctcccaatagtatgacacggtttcccagccacghChR2 (H134R)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cgccaggtcgtgaccggcatggcatggctgtttttcgtgagctggggtatgttcccaattctcttcattttggggcccgaaggttttggcgtcctgagcgtctatggctccaccgtaggtcacacgattattgatctgatgagtaaaaattgttgggggttgttgggacactaccgcggtccagcactggccgtaccgtaccgacaaaaagcactcgaccccatacaagggttaagagaagtaaaaccccgggcttccaaaaccgcaggactcgcagataccgaggtggcatccagtgtgctaataactagactactcatttttaacaacccccaacaaccctgtgatggApaIEarIPspOMIhChR2 (H134R)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tgcgcgtcctgatccacgagcacatattgattcacggagatatccgcaaaaccaccaaactgaacatcggcggaacggagatcgaggtcgagactctcgtcgaagacgaagccgaggccggagccgtgccagcggccgccgtgagcaagggcgaggagctgttcaccggggtggtacgcgcaggactaggtgctcgtgtataactaagtgcctctataggcgttttggtggtttgacttgtagccgccttgcctctagctccagctctgagagcagcttctgcttcggctccggcctcggcacggtcgccggcggcactcgttcccgctcctcgacaagtggccccaccaEcoRVPshAIhChR2 (H134R)EYFP (no start)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgccgggtaggaccagctcgacctgccgctgcatttgccggtgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtggaagccgatgccggacgtcacgBtgZIEYFP (no start)2,8202,8402,8602,8802,9002,9202,9402,960
    ttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgaagcgggcgatggggctggtgtacttcgtcgtgctgaagaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagcPfoIEYFP (no start)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    acttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccctgaagttcctcctgccgttgtaggaccccgtgttcgacctcatgttgatgttgtcggtgttgcagatatagtaccggctgttcgtcttcttgccgtagttccacttgaagttctaggcggtgttgtagctcctgccgtcgcacgtcgagcggctggtgatggtcgtcttgtggggEYFP (no start)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    catcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagagccatggcttcccgccggaggtagccgctgccggggcacgacgacgggctgttggtgatggactcgatggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggccctagtgagagccgtacctgctcgacatgttctcggtaccgaagggcggcctcBsrGIEYFP (no start)MODC Destab PEST3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gtggaggagcaggatgatggcacgctgcccatgtcttgtgcccaggagagcgggatggaccgtcaccctgcagcctgtgcttctgctaggatcaatgtggaattcgatatcaagcttatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccacacctcctcgtcctactaccgtgcgacgggtacagaacacgggtcctctcgccctacctggcagtgggacgtcggacacgaagacgatcctagttacaccttaagctatagttcgaatagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtAfeIBplIBplIClaIBglIIBspDIEcoRIEcoRVHindIIIMODC Destab PESThGH polyA3,5203,5403,5603,5803,6003,6203,6403,660
    gtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagaCspCIhGH polyA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    attgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggctaacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccgBlpINsiISphIhGH polyA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    caggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctcgtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagAloIPmlIBsaAIRsrIIBstEIIhGH polyAR-ITR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccaacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtSbfIR-ITR4,2204,2404,2604,2804,3004,3204,3404,360
    tagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaf1 Origin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgatAloIBsaAIBtgZIDraIIIf1 Origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaaagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggtPsiI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    acacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattPfoI4,9204,9404,9604,9805,0005,0205,0405,060
    tggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaEarIZraIAatIIBciVIAmpR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgXmnIAmpR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    acttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtScaIAmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgPvuIAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    aacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagAseIFspIBsrDIBsrDIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    attgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaataacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    aacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaattgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgttttttpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    accaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatctggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattagpUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    ctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactcpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    aaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagggggtttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtcccccBciVIpUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtcgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori6,8406,8606,8806,900
    • Plasmid
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    6902 bp500055006000650050010001500L-ITR,SbfI,AflIII,MluI,HindIII,PshAI,PasI,SacIIHincIIAfeI,AccIFos Truncation (after Intron 1)HincIIBsmI,SacII,EcoNIBspEI,ZraI,AatII,PsiIEcoRIBsmIEarIXmnIScaIAmpR,PvuI,BsrDIFspIAseIBsrDIpUC ori,BciVI,AflIII,PciI

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