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WT_clamp_loader
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCACTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTZraIAatIIBciVIBsaHIEcoO109IpUC19-fseq ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT20406080100120140160
    ATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCAApaLIBaeGIbla genebla signal peptideACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT180200220240260280300320340
    AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATBcgIScaIBsaHIbla geneBcgIXm...IACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT360380400420440460480500520
    CGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTAPvuIAvaIIbla geneACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT540560580600620640660680700
    ACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTTGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGABglIFspIAvaIIBsrDINmeAIIIbla geneBglIACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT720740760780800820840860880
    GATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCTATTTAGACCTCGGCCACTCGCACCAAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCAhdIBanIBmrIBpmIBsrDIBsrFIbla genepUC-remBSA-F pUC-remBSA-R Ea...IACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT9009209409609801,0001,0201,0401,060
    ACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAArep originACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT1,0801,1001,1201,1401,1601,1801,2001,2201,240
    TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATBceAIrep originRNAI transcriptACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT1,2601,2801,3001,3201,3401,3601,3801,4001,420
    GCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGAlwNIApaLIBaeGIBseYIrep originACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT1,4401,4601,4801,5001,5201,5401,5601,5801,600
    TGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCDrdIBciVIHaeIIrep originDrd...DIACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT1,6201,6401,6601,6801,7001,7201,7401,7601,780
    ATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGNspIPciIrep originACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT1,8001,8201,8401,8601,8801,9001,9201,940
    CGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGBanISapIBsaXIBspQIHaeIIpUC19-rseqCAP...ndBsaXIACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT1,9601,9802,0002,0202,0402,0602,0802,1002,120
    CCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCCGCTTCATCCAATCTCGAGCATCTTTCATTGATGGAAATTTTTGAGGAGCGCAGTTTTTACCATAGATGTCTTTGTATTTGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGGCGAAGTAGGTTAGAGCTCGTAGAAAGTAACTACCTTTAAAAACTCCTCGCGTCAAAAATGGTATCTACAGAAACATAAATliIXhoIBseRIPaeR7Iseq verifiedExtracted region from T4 genomehomologous arm 3Hom3-trunc-loc2 ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT2,1402,1602,1802,2002,2202,2402,2602,2802,300
    TGACTCTTCCTTACAATGCCTAAACATAGTTGGAAGATATTCTACTTCACGGGTACGTTCCTTTCCATTTTCATCAATATAACGTTCAACAATGTTATTTCCGACTGTTTCAATAGAGATATAAAATTCTTTCATAGATATTCCTTAGTTTATAGCCCGAGTTATTAGGCTCTTGATAACTGAGAAGGAATGTTACGGATTTGTATCAACCTTCTATAAGATGAAGTGCCCATGCAAGGAAAGGTAAAAGTAGTTATATTGCAAGTTGTTACAATAAAGGCTGACAAAGTTATCTCTATATTTTAAGAAAGTATCTATAAGGAATCAAATATCGGGCTCAATAATCCGAGAACTATBarIseq verifiedExtracted region from T4 genomehomologous arm 3ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT2,3202,3402,3602,3802,4002,4202,4402,4602,480
    TATTATACTCCAAATAAGGGGCCGAAGCCCCTTGCTTAATTACCAATCGTATATTTAGGAACGAGCTTCCATTCATGTTTTTGTTTAAAAGAAATAACTCGGAAGTTATTAGTTAAATCTTTCATAAAAGTTCTTTGACCAGGAACGATTTCAATTAGTCCCCAATCTTCTAATAGCCATAATATGAGGTTTATTCCCCGGCTTCGGGGAACGAATTAATGGTTAGCATATAAATCCTTGCTCGAAGGTAAGTACAAAAACAAATTTTCTTTATTGAGCCTTCAATAATCAATTTAGAAAGTATTTTCAAGAAACTGGTCCTTGCTAAAGTTAATCAGGGGTTAGAAGATTATCGGBsmFIseq verifiedExtracted region from T4 genomehomologous arm 3ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ATGCAATCGAATCACGACGAACTTCATCTTCTTCTGTCATTTCAACTTGACGACCATCCATACGAAGCATTTCTTTAATGGGAAATGAACGATATAGTATAGTCCTTTTTTCTGAAGAATATGACAGGACTGATATAGAACTTTATCTTTATTATTAGCAATTCCCATACGAGTCAAATACGTTAGCTTAGTGCTGCTTGAAGTAGAAGAAGACAGTAAAGTTGAACTGCTGGTAGGTATGCTTCGTAAAGAAATTACCCTTTACTTGCTATATCATATCAGGAAAAAAGACTTCTTATACTGTCCTGACTATATCTTGAAATAGAAATAATAATCGTTAAGGGTATGCTCAGTTTseq verifiedExtracted region from T4 genomehomologous arm 3GAP-Trans-clmp-F H3-rev-GAP-clampMseq...chkACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT2,6802,7002,7202,7402,7602,7802,8002,8202,840
    GTTTCTTTTACTTTCAGAAAATCTTCAGGTTTTTTAAGAGTAATTTCAATCATTTTACCATTCCAATGCTAGTTTTTTGAGTTGTTTCTGTTCTTTTACGTTCTTAGTCACTTCTTTCAAAAAATCATCCGTGACTAAACCTTTTAGTTCTTTTAATACTAAAGGAAGTTTTCCATTTCAAAGAAAATGAAAGTCTTTTAGAAGTCCAAAAAATTCTCATTAAAGTTAGTAAAATGGTAAGGTTACGATCAAAAAACTCAACAAAGACAAGAAAATGCAAGAATCAGTGAAGAAAGTTTTTTAGTAGGCACTGATTTGGAAAATCAAGAAAATTATGATTTCCTTCAAAAGGTAAAseq verifiedGP62gib.gp62.poolB.RMsq.2N.....B2.R Xm...IACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT2,8602,8802,9002,9202,9402,9602,9803,0003,020
    TTAGTAAGAATTGATTTATAGTTAATTGCATCATTTGTATTAACTTGATACCGCTTAGCAAGTAACTTAATAATCAATACTTCGGTGGAATCTTCAACCAGTTTTGCCCATTTACCATATCTTTTACCACGAGGAACTGCAGCCATTAGATAATTAAAATGAGCTTCATCACTTAAGCAATCATTCTTAACTAAATATCAATTAACGTAGTAAACATAATTGAACTATGGCGAATCGTTCATTGAATTATTAGTTATGAAGCCACCTTAGAAGTTGGTCAAAACGGGTAAATGGTATAGAAAATGGTGCTCCTTGACGTCGGTAATCTATTAATTTTACTCGAAGTAGTGAATTCGBlpIPstIAflIIseq verifiedGP62gib.gp62.poolA.RMsq.2N.GP62.B1.R Msq.3...1.2RMseq...chkMsq...F gib....FACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT3,0403,0603,0803,1003,1203,1403,1603,1803,200
    CTGATCCAATTAAATTCATAGCATATACAGCTGGCATACACTCTGGAAATTGTGATAATGCATTTTCAACCATGAATTTTGAATAATCTTTTTGAGCAATAGAGCATTTAGTTTTATTATTAATAGCTCCAATTATTTCAAAAAATTCATTTTCTGCTTTTTCTTTAAAAGAATCAGCGACTAGGTTAATTTAAGTATCGTATATGTCGACCGTATGTGAGACCTTTAACACTATTACGTAAAAGTTGGTACTTAAAACTTATTAGAAAAACTCGTTATCTCGTAAATCAAAATAATAATTATCGAGGTTAATAAAGTTTTTTAAGTAAAAGACGAAAAAGAAATTTTCTTAGTCGNsiIGP62seq verifiedgib.g...lB.FMseq.....chkACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT3,2203,2403,2603,2803,3003,3203,3403,3603,380
    AGCGGATTGGACAGCTGTCCAATCTTTTGAATACCAAGCAACTTGATGCTCGTTTAATTGAATATCATCTTTAAATAAGCTCATATCACTTCCACTGCATTTCGCATGCTAATTGAATGAAAAGATAAGCTAAATGCAATTCAGTATTAGCTGCAATACCATGATACTGATTATTTTCTCGCCTAACCTGTCGACAGGTTAGAAAACTTATGGTTCGTTGAACTACGAGCAAATTAACTTATAGTAGAAATTTATTCGAGTATAGTGAAGGTGACGTAAAGCGTACGATTAACTTACTTTTCTATTCGATTTACGTTAAGTCATAATCGACGTTATGGTACTATGACTAATAAAAGNspISphIPshAIGP44GP62gib.gp62.poolA.FMsq.4N.GP44.B3.RMseq.gp...ib.chkhomo1_f1Msq.4...1.F Ps...IACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT3,4003,4203,4403,4603,4803,5003,5203,5403,560
    GCCGACAATTTCGTACATACGAATAATACTTTGTGGAGTTACACGTGAATAGATTTCTTCGGCAAGTTTACCCACGAACCACGAATAATCAGCCGCATATTTTGGTGCTAAAGCTCTGAGTTGTTTAACATCTTTATTTTTGAGAGACTCAAGAACATCATCAATAGCACCGGATCCCCGGCTGTTAAAGCATGTATGCTTATTATGAAACACCTCAATGTGCACTTATCTAAAGAAGCCGTTCAAATGGGTGCTTGGTGCTTATTAGTCGGCGTATAAAACCACGATTTCGAGACTCAACAAATTGTAGAAATAAAAACTCTCTGAGTTCTTGTAGTAGTTATCGTGGCCTAGGGPmlIBamHIBsaAIGP4444.collar.3aa_bkbonespacer 1ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTA...ATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT3,5803,6003,6203,6403,6603,6803,7003,720
    ATAATTTCCTCATTCAAGTTTTAGTGTAAATTATAAAGGCCGAAGCCCTCTATTAAAAATCGTGGGTAGAATCAGCTTCAAGAGCTACCACATAATTCGCGTGTTCACCTTCAAATTTAGCAGCACCTTGTTTACCTTTTGCCCAAAGCAGAAGTTTATAATTTCCTGGTTGCATTTTTATTAAAGGAGTAAGTTCAAAATCACATTTAATATTTCCGGCTTCGGGAGATAATTTTTAGCACCCATCTTAGTCGAAGTTCTCGATGGTGTATTAAGCGCACAAGTGGAAGTTTAAATCGTCGTGGAACAAATGGAAAACGGGTTTCGTCTTCAAATATTAAAGGACCAACGTAAAAGP45Mseq.GP44.B1.F homo2_f1termina...reviewMsq...R ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT3,7403,7603,7803,8003,8203,8403,8603,8803,900
    CATATTTGCCATATTGATAATGAAATTAAATGTATTTTCACCATCATAATCACCAAGAGTCAAAGAATATTTAACCCGGGTCAGAGCAGAATCTTCTACTTTATTAAAACCGTTAATTACGATTTTACCTTCTTTTACCGTGATAGCAATTGTATCAATTTGCAGACCACGAGATACAGTATAAACGGTATAACTATTACTTTAATTTACATAAAAGTGGTAGTATTAGTGGTTCTCAGTTTCTTATAAATTGGGCCCAGTCTCGTCTTAGAAGATGAAATAATTTTGGCAATTAATGCTAAAATGGAAGAAAATGGCACTATCGTTAACATAGTTAAACGTCTGGTGCTCTATGTMfeISmaIXmaITspMIGP45Mseq-GP45-R ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT3,9203,9403,9603,9804,0004,0204,0404,0604,080
    CGCAACAGCTGTTGAAGGTCTTCAGCTTTAATTTCAGTAACAGCAGATGCTACCGGGAATGGAATTGGTTTATTAGGAGCAACTACTGTACTCGGATCGGCTGCTGGCCAAAAAATTGTTGAGCGGGCATCAGCAATTTTAATATTTCCATCTTCTGACTGGGAAATTTCTGCATCATGCGTTGTCGACAACTTCCAGAAGTCGAAATTAAAGTCATTGTCGTCTACGATGGCCCTTACCTTAACCAAATAATCCTCGTTGATGACATGAGCCTAGCCGACGACCGGTTTTTTAACAACTCGCCCGTAGTCGTTAAAATTATAAAGGTAGAAGACTGACCCTTTAAAGACGTAGTABbsIBmrIMscIXcmIGP45Mseq-GP45-F Msq.4N.GP45.B1.R Msq.3N...B2.F XcmIXm...IACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT4,1004,1204,1404,1604,1804,2004,2204,2404,260
    CATTAACTAAAGACAGAATACCGAGAAAACCGTTCAAATCGTAAATTGCTACATCAAAATCAATAACGTCAGAAATATTTGCTTCCGCATAAGTTGTACCATTAACTGCGCGAGTCATAATAAATTGACCGGATTTAAGCATAATACCAGAGTTAATAGTAGCGAAATTTTTAAGCAGGTAATTGATTTCTGTCTTATGGCTCTTTTGGCAAGTTTAGCATTTAACGATGTAGTTTTAGTTATTGCAGTCTTTATAAACGAAGGCGTATTCAACATGGTAATTGACGCGCTCAGTATTATTTAACTGGCCTAAATTCGTATTATGGTCTCAATTATCATCGCTTTAAAAATTCGTCGP45ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT4,2804,3004,3204,3404,3604,3804,4004,4204,440
    AGCAGTAGTATCTTTAGACAGTTTCATGTAATTTCCTTCAATTCAAATGAGATTTAATTTTATAACTAATTTAATAAAGCAATTAACGATTAAAATCAGCCGCAATTGTTTCCGCAACAATTTGAGCAGCAACAATTAGACGTTCATCTGCGGGGTAATATCAGAAAGAGACAAAACCTCGTCATCATAGAAATCTGTCAAAGTACATTAAAGGAAGTTAAGTTTACTCTAAATTAAAATATTGATTAAATTATTTCGTTAATTGCTAATTTTAGTCGGCGTTAACAAAGGCGTTGTTAAACTCGTCGTTGTTAATCTGCAAGTAGACGCCCCATTATAGTCTTTCTCTGTTTTGGMfeIBsaBIGAP2-Trans-clmp-R H4-fwd-GAP-clamp GP45Msq.4N.GP45.B1.F Extract...genomehomolog... arm 4Bs...IACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT4,4604,4804,5004,5204,5404,5604,5804,6004,620
    CATGCTCGACGAATTTTATTTTGGTTTTGTGGATTTGATTCAGAACGCACGTGTTTTGGCTGAATATCTTTTACATCAACAGTATAATTCACAGTAATTTTAGTCATAATACACCTTTAGTCATAATAATCAGTAACAGTCCAAGCTTCATTTCTATTGGACATTATTTTTGTATATTGTACGAGCTGCTTAAAATAAAACCAAAACACCTAAACTAAGTCTTGCGTGCACAAAACCGACTTATAGAAAATGTAGTTGTCATATTAAGTGTCATTAAAATCAGTATTATGTGGAAATCAGTATTATTAGTCATTGTCAGGTTCGAAGTAAAGATAACCTGTAATAAAAACATATAAAjuIAjuIPmlIBsaAIHindIIIExtracted region from T4 genomehomologous arm 4seq verifiedACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT4,6404,6604,6804,7004,7204,7404,7604,7804,800
    CTGCTTTAAATGCATTCCTAAGCATAGATTCAGTAACTATATGCTCTTCATTAGAAAAATTATTTCTCAGAATATATCGTTTTATTTCAGGAATAGTTAATAGATGCTGTCCAGTTGAATATTCCATGTTTTTCCTCCATAGAGATTATACTCTAATAAATTAAAGCATAATCTCTTAGACGAAATTTACGTAAGGATTCGTATCTAAGTCATTGATATACGAGAAGTAATCTTTTTAATAAAGAGTCTTATATAGCAAAATAAAGTCCTTATCAATTATCTACGACAGGTCAACTTATAAGGTACAAAAAGGAGGTATCTCTAATATGAGATTATTTAATTTCGTATTAGAGAATBsmINsiISapIBspQIBpu10IExtracted region from T4 genomehomologous arm 4seq verifiedACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT4,8204,8404,8604,8804,9004,9204,9404,9604,980
    TAAATTAAACCATTACAGTAAATCGACCAACTTTCTTCATTTGAAGATGCTGACCATATTCTTGCGGGTCATGGTCTTTATGCGAAATTATAAAAACGTTAGTGTTTTTCATTGAATTTATAATATTAGCTACACCTTTAATACCTTCGGCATCAAATGACCCATCAAACACTTCATCATTTAATTTGGTAATGTCATTTAGCTGGTTGAAAGAAGTAAACTTCTACGACTGGTATAAGAACGCCCAGTACCAGAAATACGCTTTAATATTTTTGCAATCACAAAAAGTAACTTAAATATTATAATCGATGTGGAAATTATGGAAGCCGTAGTTTACTGGGTAGTTTGTGAAGTAGExtracted region from T4 genomehomologous arm 4seq verifiedACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT5,0005,0205,0405,0605,0805,1005,1205,1405,160
    AAGAATTAATGTACTAATACTAACACCAGATACGATAGAAGCAATATCACGCCAAGTAAATAAAAGAGCAATATCGATTCGTGCCTTTTCACCTTCACTAAATGAAGCATAACTAAAATCTTCACGACCACGGGATTTAATTGTCTCATTAAATTCTTCATCTAATGTAAACACATAATTCTTAATTACATGATTATGATTGTGGTCTATGCTATCTTCGTTATAGTGCGGTTCATTTATTTTCTCGTTATAGCTAAGCACGGAAAAGTGGAAGTGATTTACTTCGTATTGATTTTAGAAGTGCTGGTGCCCTAAATTAACAGAGTAATTTAAGAAGTAGATTACATTTGTGTATTBtgIClaIBspDIExtracted region from T4 genomehomologous arm 4seq verifiedACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT5,1805,2005,2205,2405,2605,2805,3005,3205,340
    TCCGCTTCCATTATTTTAAGATAATGGTTAATCTGCTTATTAAATAATGGAATGTACTTTTTAATAATAGCACCTTTAATACCAGAATCTTTGAGCATATCAGTCAAAATTCCTCGGTGGTATTTTTCCATTACTAAATTAGTTTTTGTCTTAACAATTTTATCAAGTTCTTCTTGAAAGGCGAAGGTAATAAAATTCTATTACCAATTAGACGAATAATTTATTACCTTACATGAAAAATTATTATCGTGGAAATTATGGTCTTAGAAACTCGTATAGTCAGTTTTAAGGAGCCACCATAAAAAGGTAATGATTTAATCAAAAACAGAATTGTTAAAATAGTTCAAGAAGAACTTExtracted region from T4 genomehomologous arm 4seq verifiedACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTAC...TATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT5,3605,3805,4005,4205,4405,4605,4805,500
    GCAGTGCTATTTCATCAGCATGGTCAATAAACTCAGAAGATGCTTTTTCTATAGCCGCTTTAACTTTTTTAGCTTTATCTACTGCTGCGATCAGAGATTGCTTTTTATTGCGAATATCACCAGAGGTTTTCACCGTCATCACCGAAACGCGCGACGTCACGATAAAGTAGTCGTACCAGTTATTTGAGTCTTCTACGAAAAAGATATCGGCGAAATTGAAAAAATCGAAATAGATGACGACGCTAGTCTCTAACGAAAAATAACGCTTATAGTGGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTBsmBIEsp3Iseq verifiedExtracted region from T4 genomehomologous arm 4Hom4-loc1-gib pUC-bkbone-Kpn1 ACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGC...GAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAAT5,5205,5405,5605,5805,6005,6205,6405,660
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    5672 bp42504500475050005250550025050075010001250GP45rep originEcoP15IEco57I, AcuIbla geneseq verifiedDraIBauI, BssSI, Bst2BI,pUC19-fseq ,BspHI, CciI, PagI,AatII,ZraI,-35 signal,+13Eco57I, AcuI,ApaLI, Alw44I, VneI,BauI, BssSI, Bst2BI,BpuEI,+6BcgI,TaqII,ScaI,ScaI, BmcAI, ZrmI,BtsI,BcgI,ScaIBtsI,PvuI, Ple19I,TaqII,PvuI,AvaIIBsrDI, Bse3DI, BseMI,AclI, Psp1406I,Acc16I, FspI, NsbI,BsrDI,FspIVspI, AseI, PshBI,EciI,NmeAIII,BglI,NmeAIII,AvaII,BglIGsuI, BpmI,pUC-remBSA-F ,pUC-remBSA-R ,C->A removes BsaI site,+6Eam1105I, AhdI, BmeRI, DriI,AhdI,BanIDraI,DraIBspHI, CciI, PagIBpuEI,-35 signal,-10 signal,EcoP15IRNAI transcriptBceAIRNAI promoter (clockwise); GCTACA -10 signalXcmI,BmrI, BmuIBmrISspIMsq.4N.GP45.B1.F ,PsiI, AanI,BsaBI, Bse8I, BseJI,BsaBIMfeI, MunI,GAP2-Trans-clmp-R ,H4-fwd-GAP-clamp ,GAP2,MfeIAjuI,BsaAI,PmlIHindIII,HindIIIAjuIBsmINsiI,Bpu10I,BspQI,SapIExtracted region from T4 genome,homologous arm 4ClaI,BspDI,ClaI,BtgIHom4-loc1-gib ,pUC-bkbone-Kpn1 ,BsmBI,Esp3I

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    5672