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pLV-CS-146 Addgene
  • Sequence Map
    Editing disabled on read-only sequence.
    tcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgcctttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagagggttggggctcccctgggctgtccgggcttccttatcttcttcttccacctctctctctgtctctgtctaggtaagctaatcacttgcctagagctgccatagcggaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtcKflIcPPT/CTS20406080100120140160
    acatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttatcgataagcttgggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaattgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtccctgtcgtctctaggtcaaatagctattcgaaccctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttaClaIBspDICMV enhancercPPT/CTS180200220240260280300320340
    aatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgttactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtacNdeICMV enhancer360380400420440460480500520
    accttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgNcoIBsaAICspCISnaBICMV promoterCMV enhancer540560580600620640660680700
    gggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgatgaatgcatgtcatgtaccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggctacttacgtacagtacatNsiICMV promoterLNCXCMV-F720740760780800820840860
    atgctatgagaggatctatgatttccggtgaattcatgccgtaagtgtcagggtcatatggaagcggcagcgagactcccgggacctcagagtccgccacacccgaaagtgtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgttacgatactctcctagatactaaaggccacttaagtacggcattcacagtcccagtataccttcgccgtcgctctgagggccctggagtctcaggcggtgtgggctttcacactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcaDrdINdeISmaIXmaIEcoRITspMIEGFPEGFP-NBC-del1 barcode8809009209409609801,0001,0201,040
    aaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagtttgccggtgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggactggatgccgcacgtcacgaagtcggcgatggggctggtgtacttcgtcEGFP1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcgtgctgaagaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagctgaagttcctcctgccgttgtaggaccccgEGFPEXFP-R1,2401,2601,2801,3001,3201,3401,3601,3801,400
    acaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgatgttcgacctcatgttgatgttgtcggtgttgcagatatagtaccggctgttcgtcttcttgccgtagttccacttgaagttctaggcggtgttgtagctcctgccgtcgcacgtcgagcggctggtgatggtcgtcttgtgggggtagccgctgccggggcacgacgacgggctEGFP1,4201,4401,4601,4801,5001,5201,5401,560
    caaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaggatccaattctaccgggtaggggaggcgcttttcccaaggcagtctggttggtgatggactcgtgggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggccctagtgagagccgtacctgctcgacatgttcattcctaggttaagatggcccatcccctccgcgaaaagggttccgtcagacBamHIBsrGIEGFPPGK promoterEGFP-C1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcctcgtacgcgaaatcgtcggggcgacccgtgaaccgcgatgtgttcaccggagaccggagcgtgtgtaaggtgtaggtggccatccgcggttggccgaggcaagaaaccaccggggaagcgcggtggaagatgaggaggggatcagtccttcaagggggggcggggcgtcgagcgAgeISphIPGK promoterMSCV-rev1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtcgtgcaggacgtgacaaatggaagtagcacgtctcactagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggcagcacgtcctgcactgtttaccttcatcgtgcagagtgatcagagcacgtctacctgtcgtggcgactcgttaccttcgcccatccggaaaccccgtcgccggttatcgtcgaaacgaggaagcgaaagacccgagtctccgacccttccccacccaggcccccgcccgagtccBlpISpeIPGK promoter1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctgcagttaacgccgccatgaccgagtacaagcccacggtgcgcctcgccacccgcccgcccgagtccccgccccgcccgcgggcttccaggaggcctccgggccgtaagacgtgcgaagttttcgcgtgcagacggcgcgacaagaggagaaggagtagaggcccggaaagctggacgtcaattgcggcggtactggctcatgttcgggtgccacgcggagcggtgggcgHpaIPstIBfuAIBspEIBspMIMT2EY4KP6TV8RL10AT12RPuroRPGK promotermPGK-FPuro-R2,1202,1402,1602,1802,2002,2202,2402,260
    gacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgctgctgcagggggcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctgggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccacacccagcgcctgctgccgcggcSmaIXmaIBsiWIPflFIRsrIITspMIBstEIITth111ID14DV16PR18AV20RT22LA24AA26FA28DY30PA32TR34HT36VD38PD40RH42IE44RV46TE48LQ50EL52FL54TR56VG58LD60IG62KV64WV66AD68DG70APuroR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgcaccaaggaccggtggcagccgcaMscISacIIA72VA74VW76TT78PE80SV82EA84GA86VF88AE90IG92PR94MA96EL98SG100SR102LA104AQ106QQ108ME110GL112LA114PH116RP118KE120PA122WF124LA126TV128GV130PuroR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccggagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggctgcagctccacgggcttcctggcBssHIISP132DH134QG136KG138LG140SA142VV144LP146GV148EA150AE152RA154GV156PA158FL160ET162SA164PR166NL168PF170YE172RL174GF176TV178TA180DV182EV184PE186GP188PuroRPuro-F2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cgcacctggtgcatgacccgcaagcccggtgcctagacgcgtctggaacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctgcgtggaccacgtactgggcgttcgggccacggatctgcgcagaccttgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaMluISexAIAflIIIBstAPIDraIIIRT190WC192MT194RK196PG198A*200PuroRWPREWPRE-R2,8202,8402,8602,8802,9002,9202,9402,960
    ttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggatPflMIWPRE2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ttgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggcaacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagccccttcgactgcaggaaaggtaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgNcoIR4G3WPRE3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctggaattaattctgcagtcgagacctagaaaaacatggagcaatcacaagtagcaatacagggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcggaccttaattaagacgtcagctctggatctttttgtacctcgttagtgttcatcgttatgtcNaeIPstISacIINgoMIVE2I1WPRE3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaagaggggactggaagggctaattcactcccaacgaagacagtcgatggttacgactaacacggaccgatcttcgtgttctcctcctcctccacccaaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttctcccctgaccttcccgattaagtgagggttgcttctgtKpnIAcc65IBsu36I3' LTR (Delta-U3)3,5203,5403,5603,5803,6003,6203,6403,660
    agatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagaccctctagacgaaaaacgaacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggAflII3' LTR (Delta-U3)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggccttgacattgctagcgttttaccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgaaaatcagtcacaccttttagagatcgtcatcatcaagtacagtagaataataagtcataaatattgaacgtttctttacttatagtctctcactctccggaactgtaacgatcgcaaaatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacBmtINheIPsiI3' LTR (Delta-U3)M13 ReverseM13 rev3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcclac promoterCAP binding siteM13/p...erselac operator4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccgPciISapIBspQIAflIIIL44404,2204,2404,2604,2804,3004,3204,3404,360
    caggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcDrdIoripBR322ori-F4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccagaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggtori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    acccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagtgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttcori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    agttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagatcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctBspHIori4,9204,9404,9604,9805,0005,0205,0405,060
    ttatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacaAhdI*W286HK284IL282SA280GI278EA276IQ274RN272RE270DM268TA266QS264GT262TY260AmpR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    agataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgtctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcIV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202AmpR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagAclIFspISA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170EN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144AmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgccccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacggPvuIScaIPG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    cggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtgccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaAclIA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TAmpRAmp-R5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatagggaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatcccSspIBspHIE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2MAmpRAmpR promoter5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatctcaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaPsiISgrDISV40 poly(A) signalSV40pA-REBV-rev6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tatcatgtctggatcaactggataactcaagctaaccaaaatcatcccaaacttcccaccccataccctattaccactgccaattacctgtggtttcatttactctaaacctgtgattcctctgaattattttcattttaaagaaattgtatttgttaaatatgtactacaaactatagtacagacctagttgacctattgagttcgattggttttagtagggtttgaagggtggggtatgggataatggtgacggttaatggacaccaaagtaaatgagatttggacactaaggagacttaataaaagtaaaatttctttaacataaacaatttatacatgatgtttgaSV40...nal6,3206,3406,3606,3806,4006,4206,4406,460
    tagtagttggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagccagataaggatcatcaaccttcccgattaagtgagggtttcttctgttctataggaactagacacctagatggtgtgtgttccgatgaagggactaatcgtcttgatgtgtggtcccggtccccagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcggtctattcc3' LTR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tagaagaggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaactgctgatatcgagcttgatcttctccggttatttcctctcttgtggtcgaacaatgtgggacactcggacgtaccctacctactgggcctctctcttcacaatctcacctccaaactgtcggcggatcgtaaagtagtgcaccgggctctcgacgtaggcctcatgaagttcttgacgactatagctcgaacPmlIScaIBsaAIBspEIDraIII3' LTR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactggggagtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctgatgttccctgaaaggcgacccctgaaaggtccctccgcaccggacccgccctgacccctcaccgctcgggagtctaggacgtatattcgtcgacgaaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaAflII3' LTR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    taagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagagagctgcgtBplI3' LTRHIV...si7,0207,0407,0607,0807,1007,1207,1407,160
    ggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaacctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtccccctttBplINruIBssHIIHIV-1 Psi7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaacccttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgttgg7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataagagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatattFseIMfeINaeISapIBspQIEcoNINgoMIV7,5407,5607,5807,6007,6207,6407,6607,6807,700
    agtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacRRE7,7207,7407,7607,7807,8007,8207,8407,860
    tctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttagaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaaBbvCIBstAPIRRE7,8807,9007,9207,9407,9607,9808,0008,0208,040
    gcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggacgtggtgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataacctK1N2E3Q4E5L6L7E8gp41 peptide8,0608,0808,1008,1208,1408,1608,1808,2008,220
    attagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccactaatctatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtgL9D10K11W12A13S14L15gp41 peptide8,2408,2608,2808,3008,3208,3408,3608,3808,400
    cg8,401
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8401 bp60006500700075008000500100015002000PGK promoterMSCV-revgp41 peptidecPPT/CTS,CMV enhancer,KflI,BspDI,ClaICMV promoter,NdeI,BsaAI,SnaBI,NcoI,CspCICMV-F,LNCX,AUG,PAM,BC-del1 barcode,NsiI,EcoRI,NdeI,TspMI,XmaI,SmaIDrdIEGFP-NEXFP-REGFPEGFP-C,BsrGI,BamHISphI,AgeISpeIBlpIDraIII3' LTR,BsaAI,PmlI,BspEI,ScaIAflIIHIV-1 Psi,BplI,BplI,BssHIINruIBspQI,SapI,NgoMIV,NaeI,FseI,EcoNIMfeIRRE,BbvCI,BstAPI

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    8401

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