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(pEmpty_Control = pKR484 ) pRS416-tRNA-HDV-NotI-Qi-gRNA + Tef1-Cas9 with RPL25 intron
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCCTGCCTAGCGAACGGACATTGAATGTGCGCGGAGCATAGAAAATTACTACCTTATTAAACCCTTAAATGAGACACAAATAAATAAAAATACAAAACATAAACCTAAAATCCEN/ARS20406080100120140160
    AAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAATCACAGGATTTTCGTTTCATTTATTTCTTCCATCTTCTCAATGCCTTACTTCTTTTTTTTTATTTGTTTCCAAATTTTTTAAAGTTGTTTTTCGCATGAAATGTATATATAAATAATCTGTTCTTTTCGTCTAATTTATCTATATGTAAGCTAATTGCTATTCATTTTACATTTTAGTGTCCTAAAAGCCEN/ARS180200220240260280300320340
    TGTGTGGTCTTCTACACAGACAAGATGAAACAATTCGGCATTAATACCTGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAACACACCAGAAGATGTGTCTGTTCTACTTTGTTAAGCCGTAATTATGGACTCTCGTCCTTCTCGTTCTATTTTCCATCATAAACAACCGCTAGGGGGATCTCAGAAAATGTAGAAGCCTTTTGTTTTTGATAAAAAAGAAATTAAAGAAAAAAATGAAAGATAAAAATTAAATATCEN/ARS360380400420440460480500520
    TATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATGAAAAGGACCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTATAAATATAATTTTTTAAATTTAATATTAATAAAAATATCGTGCACTACTTTTCCTGGGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAAPmlISwaIAmpR promoterCEN/ARS540560580600620640660680700
    GAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAAmpR open reading frame (ORF)AmpR...ter720740760780800820840860
    CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCAmpR open reading frame (ORF)8809009209409609801,0001,0201,040
    GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCACTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTAmpR open reading frame (ORF)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATANmeAIIIAmpR open reading frame (ORF)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTAhdIAmpR open reading frame (ORF)1,4201,4401,4601,4801,5001,5201,5401,560
    CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCAmpR terminator1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGori2,1202,1402,1602,1802,2002,2202,2402,260
    ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCTTAATTAAGTATATGTGTTATGTAGTATACTCTTTCTTCAACAATTAAATACTCTCGGTAGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGAATTAATTCATATACACAATACATCATATGAGAAAGAAGTTGTTAATTTATGAGAGCCATCPacIoritRNA-Tyr (SUP4)US_gRNA_fwd2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCAAGTTGGTTTAAGGCGCAAGACTGTAATTTATCACTACGAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGAGATGGCCGGCATGGTCCCAGCCTCCTCGCTGGCGCCGGCTGGGCAACACCTTCGGGTGGCGAATGGGACTTTGGCGGCCGCgtttaagagctatgcGGTTCAACCAAATTCCGCGTTCTGACATTAAATAGTGATGCTTTAGAACTCTAGCCCGCAAGCTGAGCGGGGGCCCTCTCTACCGGCCGTACCAGGGTCGGAGGAGCGACCGCGGCCGACCCGTTGTGGAAGCCCACCGCTTACCCTGAAACCGCCGGCGcaaattctcgatacgAjuINotItRNA-Tyr (SUP4)HDV-RibozymegRNA_NotI_revCas9...old2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tggaaacagcatagcaagtttaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttGTTAACTCCGTTACTGTGGGCTTGCCATATTTTTTGGAACTTTTCTGCCCTTTTTCTCAATGAGTAAGGAGGGCGTTTATCTTTTCTATTTTATTTTTacctttgtcgtatcgttcaaatttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaCAATTGAGGCAATGACACCCGAACGGTATAAAAAACCTTGAAAAGACGGGAAAAAGAGTTACTCATTCCTCCCGCAAATAGAAAAGATAAAATAAAAAHpaIU5 terminator (SNR7)Cas9 gRNA scaffold2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CTACAAATTTGATTCATGAGTCCATGGAACAAATATATAGAACTCATTTTCTTGTTTCGGTATATATATTAACTCCATTGAAGCATTTGCTTTTATCCTGACTGGCTAGCtagtcgtgcaatgtatgactttaagCCGCGGTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAGATGTTTAAACTAAGTACTCAGGTACCTTGTTTATATATCTTGAGTAAAAGAACAAAGCCATATATATAATTGAGGTAACTTCGTAAACGAAAATAGGACTGACCGATCGatcagcacgttacatactgaaattcGGCGCCAGAAAGGACGCAATAGGGGACTAAGACACCTATTBmtINcoINheISacIIU5 terminator (SNR7)DS_gRNA_rev2,8202,8402,8602,8802,9002,9202,9402,960
    CCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCG2,9803,0003,0203,0403,0603,0803,1003,1203,140
    AGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTcaCACAggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAgtGTGTcctttgtcgatactggtactaatgcggttcgcgcgttaattgggagtgatttcccttgT3_promoterM13_rev3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aaaagctggAGCTCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAttttcgaccTCGAGTATCGAAGTTTTACAAAGATGAGGAAAAAATGAGAAGGTCTAAAAGAGCCTGAGGCGCGTAGCGGCATGGTGAAGTTTTGTGGGTTCGTGTCGTATGATTTAAAGGGGAGAAAGAAGGAGATCCCACAGCAATTAATGGGCATGATTTCCAAACCTTTTCTSacIEco53kITEF Promoter3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTTTTCTCTGGCGGAGCAAAGAAAAAGAAGCAGCTTTTTCCGTTATTTTTAAAAATAGTGCAAAGAAAAAGAACTTTTAAAAAAAAAACTAAAAAAAGAGAAAGCTACTGGAGGGTAACTATAAATTCAATTATTTGCCAGAAGTTAAAGAGTTCAAAGTCAAAGTAAAAAGAACTEF Promoter3,5203,5403,5603,5803,6003,6203,6403,660
    TTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTCTAGAGatgtctagagccccaaagaagaagagaaaagttagacccgggATGGACAAGAAGTACTCCATTGGGCTCGATATCGGCACAAACAGCGTCGGCTGGGCCGTCAAAGATAATGTTGAAAAAAATGAAGAACGAGTAATCTTTCTTTCGTATCGTTAGATTAGATTCAAAAGATCTCtacagatctcggggtttcttcttctcttttcaatctgggcccTACCTGTTCTTCATGAGGTAACCCGAGCTATAGCCGTGTTTGTCGCAGCCGACCCGGCAGTXbaIXbaIEcoRVM1S2R3A4P5K6K7K8R9K10V11R12P13G143748-3789MD2KK4YS6IG8LD10IG12TN14SV16GW18AV203790-8343Cas9 exon 1TEF Promoternuclear localizati...(NLS) with linker3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGTTCTGGGCAATACCGATCGCCACAGCATAAAGAAGAACCTCATTGGCGCCCTCCTGTTCGACTCCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCGCAGAAAGAATCGGATAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACCCGTTATGGCTAGCGGTGTCGTATTTCTTCTTGGAGTAACCGCGGGAGGACAAGCTGAGGCCCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTTTCTTAGCCTANmeAIIIIT22DE24YK26VP28SK30KF32KV34LG36NT38DR40HS42IK44KN46LI48GA50LL52FD54SG56ET58AE60AT62RL64KR66TA68RR70RY72TR74RK76NR78I3790-8343Cas9 exon 13,8603,8803,9003,9203,9403,9603,9804,0004,020
    CTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAGGTGGATGACTCTTTCTTCCATAGGCTGGAGGAGTCCTTTTTGGTGGAGGAGGATAAAAAGCACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTACCCAACCATATATCATGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGAGAAAGAAGGTATCCGACCTCCTCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGGTGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATATAGTASbfIXcmIBglIIPflMIPflMIC80YL82QE84IF86SN88EM90AK92VD94DS96FF98HR100LE102ES104FL106VE108ED110KK112HE114RH116PI118FG120NI122VD124EV126AY128HE130KY132PT134IY136H3790-8343Cas9 exon 14,0404,0604,0804,1004,1204,1404,1604,1804,200
    CTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACTTGCGGTTGATCTATCTCGCGCTGGCGCATATGATCAAATTTCGGGGACACTTCCTCATCGAGGGGGACCTGAACCCAGACAACAGCGATGTCGACAAACTCTTTATCCAACTGGTTCAGACTTACAATCAGCTTTGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAGAGCGCGACCGCGTATACTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGGGTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGTCGAAANdeISalIEcoNIL138RK140KL142VD144ST146DK148AD150LR152LI154YL156AL158AH160MI162KF164RG166HF168LI170EG172DL174NP176DN178SD180VD182KL184FI186QL188VQ190TY192NQ194L3790-8343Cas9 exon 14,2204,2404,2604,2804,3004,3204,3404,360
    TCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAAGCAATCCTGAGCGCTAGGCTGTCCAAATCCCGGCGGCTCGAAAACCTCATCGCACAGCTCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCTGACCCCCAACTTTAAATCAGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCGACAGGTTTAGGGCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGCCGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAATTTAGPasIBspEIF196EE198NP200IN202AS204GV206DA208KA210IL212SA214RL216SK218SR220RL222EN224LI226AQ228LP230GE232KK234NG236LF238GN240LI242AL244SL246GL248TP250NF252KS2543790-8343Cas9 exon 14,3804,4004,4204,4404,4604,4804,5004,5204,540
    TAACTTCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGCAAAGACACCTACGATGATGATCTCGACAATCTGCTGGCCCAGATCGGCGACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAGTGATATTCTGCGAGTGAACACGGAGATCATTGAAGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGCTGTTAGACGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGGACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCCTCTAGBsaBINF256DL258AE260DA262KL264QL266SK268DT270YD272DD274LD276NL278LA280QI282GD284QY286AD288LF290LA292AK294NL296SD298AI300LL302SD304IL306RV308NT310EI3123790-8343Cas9 exon 14,5604,5804,6004,6204,6404,6604,6804,7004,720
    ACCAAAGCTCCGCTGAGCGCTAGTATGATCAAGCGCTATGATGAGCACCACCAAGACTTGACTTTGCTGAAGGCCCTTGTCAGACAGCAACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGGATACATTGACGGCGGAGCAAGCCAGGAGGTGGTTTCGAGGCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGACTTCCGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGATTTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGTCCTCCBlpITK314AP316LS318AS320MI322KR324YD326EH328HQ330DL332TL334LK336AL338VR340QQ342LP344EK346YK348EI350FF352DQ354SK356NG358YA360GY362ID364GG366AS368QE3703790-8343Cas9 exon 14,7404,7604,7804,8004,8204,8404,8604,8804,900
    AATTTTACAAATTTATTAAGCCCATCTTGGAAAAAATGGACGGCACCGAGGAGCTGCTGGTAAAGCTTAACAGAGAAGATCTGTTGCGCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACTGCACGCTATCCTCAGGCGGCAAGAGGATTTTTAAAATGTTTAAATAATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGTCTCTTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGGACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTCCTAAABglIIBsu36IEF372YK374FI376KP378IL380EK382MD384GT386EE388LL390VK392LN394RE396DL398LR400KQ402RT404FD406NG408SI410PH412QI414HL416GE418LH420AI422LR424RQ426ED428F3790-8343Cas9 exon 14,9204,9404,9604,9805,0005,0205,0405,060
    CTACCCCTTTTTGAAAGATAACAGGGAAAAGATTGAGAAAATCCTCACATTTCGGATACCCTACTATGTAGGCCCCCTCGCCCGGGGAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAACTTCGAGGAAGTCGTGGATAAGGGGGCCTCTGATGGGGAAAAACTTTCTATTGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCGGGGGAGCGGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGACCTTGAAGCTCCTTCAGCACCTATTCCCCCGGAGAY430PF432LK434DN436RE438KI440EK442IL444TF446RI448PY450YV452GP454LA456RG458NS460RF462AW464MT466RK468SE470ET472IT474PW476NF478EE480VV482DK484GA486S3790-8343Cas9 exon 15,0805,1005,1205,1405,1605,1805,2005,2205,240
    GCCCAGTCCTTCATCGAAAGGATGACTAACTTTGATAAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGCTGTACGAGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAAAGCCAGCATTCCTGTCTGGAGAGCAGAAGACGGGTCAGGAAGTAGCTTTCCTACTGATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGACGACATGCTCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTTTCGGTCGTAAGGACAGACCTCTCGTCTTCTBarISacIBsaBIEco53kIA488QS490FI492ER494MT496NF498DK500NL502PN504EK506VL508PK510HS512LL514YE516YF518TV520YN522EL524TK526VK528YV530TE532GM534RK536PA538FL540SG542EQ544K3790-8343Cas9 exon 15,2605,2805,3005,3205,3405,3605,3805,4005,420
    AAGCTATCGTGGACCTCCTCTTCAAGACGAACCGGAAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATTGAATGTTTCGACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACGATCTCCTGAAAATCATTAAAGACAATTCGATAGCACCTGGAGGAGAAGTTCTGCTTGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTAACTTACAAAGCTGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGCTAGAGGACTTTTAGTAATTTCTGTTPasIK546AI548VD550LL552FK554TN556RK558VT560VK562QL564KE566DY568FK570KI572EC574FD576SV578EI580SG582VE584DR586FN588AS590LG592TY594HD596LL598KI600IK602DK6043790-8343Cas9 exon 15,4405,4605,4805,5005,5205,5405,5605,5805,600
    GGACTTCCTGGACAATGAGGAGAACGAGGACATTCTTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGATGATTGAAGAACGCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCGCCGATATACAGGATGGGGGCGGCTGCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAGAACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCTACTAACTTCTTGCGAACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTATATGTCCTACCCCCGCCGACPflFIPflFITth111ITth111IDF606LD608NE610EN612ED614IL616ED618IV620LT622LT624LF626ED628RE630MI632EE634RL636KT638YA640HL642FD644DK646VM648KQ650LK652RR654RY656TG658WG660RL6623790-8343Cas9 exon 15,6205,6405,6605,6805,7005,7205,7405,760
    TCAAGAAAACTGATCAATGGGGTATGTGAACTGCAATATTAATAGCACGAGAAAATTGAGAGGAAGATAGATGGGAACTAGTAGAGTTGATATTGATGAGATACGAAAACCACACGTAAATAAACTATCCGACGACAAGAATAGTGCATTAAGGACTTGATTAAGATATGGTGAGAGTTCTTTTGACTAGTTACCCCATACACTTGACGTTATAATTATCGTGCTCTTTTAACTCTCCTTCTATCTACCCTTGATCATCTCAACTATAACTACTCTATGCTTTTGGTGTGCATTTATTTGATAGGCTGCTGTTCTTATCACGTAATTCCTGAACTAATTCTATACCACTCSpeISR664KL666IN668GV670CE672LQ674Y*676*H678EK680IE682RK684ID686GN688**690S*692Y*694*D696TK698TT700RK702*T704IR706RQ708E*710CI712KD714LI716KI718W*7203790-8343RPL25 IntronCas9 exon 15,7805,8005,8205,8405,8605,8805,9005,9205,940
    CAACGTAATTATCGGGCTCAACAGTTTATTAGCAATCGTTTTGATAGAAGCGTTGATGCTGTGGAAGTTGTTCTTTTTACTACCAACAGCCATTAACAAATCCATTAGAGTGTTCGTTCGTTTTTCTGCTCAGATTCAAGAAAATTATTCCATCCTCATTATACTTTTTTCTTCTGTTGCATTAATAGCCCGAGTTGTCAAATAATCGTTAGCAAAACTATCTTCGCAACTACGACACCTTCAACAAGAAAAATGATGGTTGTCGGTAATTGTTTAGGTAATCTCACAAGCAAGCAAAAAGACGAGTCTAAGTTCTTTTAATAAGGTAGGAGTAATATGAAAAAAGAAGAAT722*L724SG726ST728VY730*Q732SF734**736KR738*C740CG742SC744SF746YY748QQ750PL752TN754PL756EC758SF760VF762LL764RF766KK768II770PS772SL774YF776FL778L3790-8343RPL25 Intron5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ATTTCGTGCTCCACGTCGAGGTATCAAGGAACATAGTTTACTAACATTAACGAATTCATCTCCTATGAATTTACTTTTTGTATAGATCCGAGACAAGCAGAGTGGAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGCCAACCGGAACTTCATGCAGTTGATCCATGATTAAAGCACGAGGTGCAGCTCCATAGTTCCTTGTATCAAATGATTGTAATTGCTTAAGTAGAGGATACTTAAATGAAAAACATATCTAGGCTCTGTTCGTCTCACCTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACGGTTGGCCTTGAAGTACGTCAACTAGGTACTAAjuIPsrIAflIIBmgBICspCIEcoRIF780RA782PR784RG786IK788EH790SL792LT794LT796NS798SP800MN802LL804FV806*I808RD810KQ812SG814KT816IL818DF820LK822SD824GF826AN828RN830FM832QL834IH836D3790-8343Cas9 exon 2RPL25 Intron6,1406,1606,1806,2006,2206,2406,2606,2806,300
    GACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACAAGTTTCTGGCCAGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCAGCTATCAAAAAGGGAATACTGCAGACCGTTAAGGTCGTGGATGAACTCGTCAAAGTAATGGGAAGGCATAAGCCCGAGACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGGTCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTTTTTCCCTTATGACGTCTGGCAATTCCAGCACCTACTTGAGCAGTTTCATTACCCTTCCGTATTCGGGCTCTD838SL840TF842KE844DI846QK848AQ850VS852GQ854GD856SL858HE860HI862AN864LA866GS868PA870IK872KG874IL876QT878VK880VV882DE884LV886KV888MG890RH892KP894E3790-8343Cas9 exon 26,3206,3406,3606,3806,4006,4206,4406,460
    ATATCGTTATCGAGATGGCCCGAGAGAACCAAACTACCCAGAAGGGACAGAAGAACAGTAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAAGAACTGGGGTCCCAAATCCTTAAGGAACACCCAGTTGAAAACACCCAGCTTCAGAATGAGAAGCTCTACCTGTACTACCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCTTGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCCAGGGTTTAGGAATTCCTTGTGGGTCAACTTTTGTGGGTCGAAGTCTTACTCTTCGAGATGGACATGATGGAKflIAflIIN896IV898IE900MA902RE904NQ906TT908QK910GQ912KN914SR916ER918MK920RI922EE924GI926KE928LG930SQ932IL934KE936HP938VE940NT942QL944QN946EK948LY950LY952YL9543790-8343Cas9 exon 26,4806,5006,5206,5406,5606,5806,6006,6206,640
    GCAGAACGGCAGGGACATGTACGTGGATCAGGAACTGGACATCAATCGGCTCTCCGACTACGACGTGGATCATATCGTGCCCCAGTCTTTTCTCAAAGATGATTCTATTGATAATAAAGTGTTGACAAGATCCGATAAAAATAGAGGGAAGAGTGATAACGTCCCCTCAGAAGAACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGATGCTGCACCTAGTATAGCACGGGGTCAGAAAAGAGTTTCTACTAAGATAACTATTATTTCACAACTGTTCTAGGCTATTTTTATCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTPciIBmgBIQN956GR958DM960YV962DQ964EL966DI968NR970LS972DY974DV976DH978IV980PQ982SF984LK986DD988SI990DN992KV994LT996RS998DK1000NR1002GK1004SD1006NV1008PS1010EE10123790-8343Cas9 exon 26,6606,6806,7006,7206,7406,7606,7806,8006,820
    GTTGTCAAGAAAATGAAAAATTATTGGCGGCAGCTGCTGAACGCCAAACTGATCACACAACGGAAGTTCGATAATCTGACTAAGGCTGAACGAGGTGGCCTGTCTGAGTTGGATAAAGCCGGCTTCATCAAAAGGCAGCTTGTTGAGACACGCCAGATCACCAAGCACGTGGCCCCAACAGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCTTCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGACTCAACCTATTTCGGCCGAAGTAGTTTTCCGTCGAACAACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGPmlIBstXIVV1014KK1016MK1018NY1020WR1022QL1024LN1026AK1028LI1030TQ1032RK1034FD1036NL1038TK1040AE1042RG1044GL1046SE1048LD1050KA1052GF1054IK1056RQ1058LV1060ET1062RQ1064IT1066KH1068VA10703790-8343Cas9 exon 26,8406,8606,8806,9006,9206,9406,9606,9807,000
    AAATTCTCGATTCACGCATGAACACCAAGTACGATGAAAATGACAAACTGATTCGAGAGGTGAAAGTTATTACTCTGAAGTCTAAGCTGGTCTCAGATTTCAGAAAGGACTTTCAGTTTTATAAGGTGAGAGAGATCAACAATTACCACCATGCGCATGATGCCTACCTGAATGCTTTAAGAGCTAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAATAATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCCTGAAAGTCAAAATATTCCACTCTCTCTAGTTGTTAATGGTGGTACGCGTACTACGGATGGACTTACGPsrIFspAIQI1072LD1074SR1076MN1078TK1080YD1082EN1084DK1086LI1088RE1090VK1092VI1094TL1096KS1098KL1100VS1102DF1104RK1106DF1108QF1110YK1112VR1114EI1116NN1118YH1120HA1122HD1124AY1126LN1128A3790-8343Cas9 exon 27,0207,0407,0607,0807,1007,1207,1407,160
    AGTGGTAGGCACTGCACTTATCAAAAAATATCCCAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGTACGATGTTAGGAAAATGATCGCAAAGTCTGAGCAGGAAATAGGCAAGGCCACCGCTAAGTACTTCTTTTACAGCAATATTATGAATTTTTTCAAGACCGAGTCACCATCCGTGACGTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACATGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCTTTATCCGTTCCGGTGGCGATTCATGAAGAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCV1130VG1132TA1134LI1136KK1138YP1140KL1142ES1144EF1146VY1148GD1150YK1152VY1154DV1156RK1158MI1160AK1162SE1164QE1166IG1168KA1170TA1172KY1174FF1176YS1178NI1180MN1182FF1184KT1186E3790-8343Cas9 exon 27,1807,2007,2207,2407,2607,2807,3007,3207,340
    ATTACACTGGCCAATGGAGAGATTCGGAAGCGACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACAAGGGTAGGGATTTCGCGACAGTCCGGAAGGTCCTGTCCATGCCGCAGGTGAACATCGTTAAAAAGACCGAAGTACAGACCGGAGGCTTCTCCAAGGTAATGTGACCGGTTACCTCTCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGTTCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCAATTTTTCTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCNruIBspEIPaqCII1188TL1190AN1192GE1194IR1196KR1198PL1200IE1202TN1204GE1206TG1208EI1210VW1212DK1214GR1216DF1218AT1220VR1222KV1224LS1226MP1228QV1230NI1232VK1234KT1236EV1238QT1240GG1242FS1244K3790-8343Cas9 exon 27,3607,3807,4007,4207,4407,4607,4807,5007,520
    AAAGTATCCTCCCGAAAAGGAACAGCGACAAGCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTCGATTCTCCTACAGTCGCTTACAGTGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAGTCTAAAAAACTCAAAAGCGTCAAGGAACTGCTGGGCATTTTCATAGGAGGGCTTTTCCTTGTCGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAAGAGGATGTCAGCGAATGTCACATGACCAACACCGGTTTCACCTCTTTCCCTTCAGATTTTTTGAGTTTTCGCAGTTCCTTGACGACCCGTAKflIE1246SI1248LP1250KR1252NS1254DK1256LI1258AR1260KK1262DW1264DP1266KK1268YG1270GF1272DS1274PT1276VA1278YS1280VL1282VV1284AK1286VE1288KG1290KS1292KK1294LK1296SV1298KE1300LL1302GI13043790-8343Cas9 exon 27,5407,5607,5807,6007,6207,6407,6607,6807,700
    CACAATCATGGAGCGATCAAGCTTCGAAAAAAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAAGACCTCATCATTAAGCTTCCCAAGTACTCTCTCTTTGAGCTTGAAAACGGCCGGAAACGAATGCTCGCTAGTGCGGGCGAGCTGCAGAAAGGTAACGTGTTAGTACCTCGCTAGTTCGAAGCTTTTTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGTAATTCGAAGGGTTCATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTGCTTACGAGCGATCACGCCCGCTCGACGTCTTTCCATTGTliIXhoIPaeR7ITI1306ME1308RS1310SF1312EK1314NP1316ID1318FL1320EA1322KG1324YK1326EV1328KK1330DL1332II1334KL1336PK1338YS1340LF1342EL1344EN1346GR1348KR1350ML1352AS1354AG1356EL1358QK1360GN13623790-8343Cas9 exon 27,7207,7407,7607,7807,8007,8207,8407,860
    GAGCTGGCACTGCCCTCTAAATACGTTAATTTCTTGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAAGATAATGAGCAGAAGCAGCTGTTCGTGGAACAACACAAACACTACCTTGATGAGATCATCGAGCAAATAAGCGAATTCTCCAAAAGAGTGATCCTCGCTCGACCGTGACGGGAGATTTATGCAATTAAAGAACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCGTCGACAAGCACCTTGTTGTGTTTGTGATGGAACTACTCTAGTAGCTCGTTTATTCGCTTAAGAGGTTTTCTCACTAGGAGCEcoRIEL1364AL1366PS1368KY1370VN1372FL1374YL1376AS1378HY1380EK1382LK1384GS1386PE1388DN1390EQ1392KQ1394LF1396VE1398QH1400KH1402YL1404DE1406II1408EQ1410IS1412EF1414SK1416RV1418IL14203790-8343Cas9 exon 27,8807,9007,9207,9407,9607,9808,0008,0208,040
    CCGACGCTAACCTCGATAAGGTGCTTTCTGCTTACAATAAGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAACATTATCCACTTGTTTACTCTGACCAACTTGGGCGCGCCTGCAGCCTTCAAGTACTTCGACACCACCATAGACAGAAAGCGGTACACCTCTACAAAGGAGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTATTCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACTGGTTGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTGTGGTGGTATCTGTCTTTCGCCATGTGGAGATGTTTCCTAscIEcoNIAD1422AN1424LD1426KV1428LS1430AY1432NK1434HR1436DK1438PI1440RE1442QA1444EN1446II1448HL1450FT1452LT1454NL1456GA1458PA1460AF1462KY1464FD1466TT1468ID1470RK1472RY1474TS1476TK1478E3790-8343Cas9 exon 28,0608,0808,1008,1208,1408,1608,1808,2008,220
    GGTCCTGGACGCCACACTGATTCATCAGTCAATTACGGGGCTCTATGAAACAAGAATCGACCTCTCTCAGCTCGGTGGAGACAGCAGGGCTGACCCCAAGAAGAAGAGGAAGGTGTGATCTCTTCTCGAGTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGATACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGGGGTTCTTCTTCTCCTTCCACACTAGAGAAGAGCTCAGTACATTAATCAATACAGTGCGAATGTAAGTGCGGGAGGGGGGTTliIXhoIPaeR7IV1480LD1482AT1484LI1486HQ1488SI1490TG1492LY1494ET1496RI1498DL1500SQ1502LG1504GD1506SR1508AD1510PK1512KK1514RK1516V*15183790-8343Cas9 exon 2CYC1 Terminator8,2408,2608,2808,3008,3208,3408,3608,3808,400
    CATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTGTAGGCGAGATTGGCTTTTCCTTCCTCAATCTGTTGGACTTCAGATCCAGGGATAAATAAAAAAATATCAATACAATCATAATTCTTGCAATAAATATAAAGTTTAAAAAGAAAAAAAAGACATGTCTGCGCACATGCGTACATTGTAATATGACTTTTGGAACGAACTCTTCCAMluIBsrGICYC1 Terminator8,4208,4408,4608,4808,5008,5208,5408,560
    TTTGGGACGCTCGAAGGCTTTAATTTGCGGCCGGTACccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAACCCTGCGAGCTTCCGAAATTAAACGCCGGCCATGggttaagcgggatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgtTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCKpnIAcc65IlacZαCYC1 TerminatorT7T7_promoterM13-fwd8,5808,6008,6208,6408,6608,6808,7008,7208,740
    AAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAlacZαf1 ori8,7608,7808,8008,8208,8408,8608,8808,9008,920
    CGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGf1 orilacZα8,9408,9608,9809,0009,0209,0409,0609,0809,100
    TTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCCTGATGCGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTAAAGGACTACGCAloIf1 ori9,1209,1409,1609,1809,2009,2209,2409,260
    GTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAGGGTAATAACTGATATAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTTTAGTTTTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCCTGCTTTTCTGTACATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATCCCATTATTGACTATATTAATTTAACTTCGAGATTAAACACTCAAATCATATGTACGTAAATGAATATTATGTCAAAAAATCAAAACGACCGGCGTAGAAGAGTTTATACGAAGGGTCGGACGAAAAGACATNsiI*268NQ266QG264CR262RL260YA258EW256GA254KR252Y9397-10200URA3 terminatorURA39,2809,3009,3209,3409,3609,3809,4009,4209,440
    ACGTTCACCCTCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAATAATAATGTCAGATCCTGTAGAGACCACATCATCCACGGTTCTATACTGTTGACCCAATGCGTCTCCCTTGTCATCTAAACCCACACCGGGTGTCATAATCAACCAATCGTAACCTTCATGCAAGTGGGAGATGGAATCGTAGGGAAGGGAAACGTTTATCAGGAGAAGGTTGTTATTATTACAGTCTAGGACATCTCTGGTGTAGTAGGTGCCAAGATATGACAACTGGGTTACGCAGAGGGAACAGTAGATTTGGGTGTGGCCCACAGTATTAGTTGGTTAGCATTGGAAGTR250EG248EV246KA244DR242GK240AF238LG236RG234VI232II230DS228GT226SV224VD222DV220TR218YQ216QG214LA212DG210KD208DL206GV204GP202TM200IL198WD196YG194E9397-10200URA39,4609,4809,5009,5209,5409,5609,5809,6009,620
    TCTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAATCTTTGTCGCTCTTCGCAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATGACAATTCTGCTAACATCAAAAGGCCTCTAGGTTCCTTTGTTACTTCTTCTGCCGCCTGCTAGAGAAGGTGGGTACAGAGAAACTCGTTATTTCGGCTATTGTTTTAGAAACAGCGAGAAGCGTTACAGTTGTCATGGGAATCATATAAGAGGTCATCTATCCCTCGGGAACGTACTGTTAAGACGATTGTAGTTTTCCGGAGATCCAAGGAAACAATGAAGAAGACGGCGGACGAStuID192RG190GM188DR186QA184IF182GI180VF178DK176DS174KA172ID170VT168GK166TY164EG162TS160LS158GK156CS154LE152AL150ML148LG146RP144EK142TV140EE138AA136QK1349397-10200URA39,6409,6609,6809,7009,7209,7409,7609,7809,800
    TCAAACCGCTAACAATACCTGGGCCCACCACACCGTGTGCATTCGTAATGTCTGCCCATTCTGCTATTCTGTATACACCCGCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTCTGTCTTCGAAGAGTAAAAAATTGTACTTGGCGGATAATGCCTTTAGCGGAGTTTGGCGATTGTTATGGACCCGGGTGGTGTGGCACACGTAAGCATTACAGACGGGTAAGACGATAAGACATATGTGGGCGTCTCATGACGTTAAACTGACATAATGGTTACAGTCGTTTAAAAGACAGAAGCTTCTCATTTTTTAACATGAACCGCCTATTACGGAAATCGCCApaIPspOMILG132SV130IG128PG126VV124GH122AN120TI118DA116WE114AI112RY110VG108AS106YQ104LK102VT100NG98ID96AF94KR92DE90FL88LF86NY84KA82SL80AK78LP769397-10200URA39,8209,8409,8609,8809,9009,9209,9409,960
    CTTAACTGTGCCCTCCATGGAAAAATCAGTCAAGATATCCACATGTGTTTTTAGTAAACAAATTTTGGGACCTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACATCCAATGAAGCACACAAGTTTGTTTGCTTTTCGTGCATGATATTAAATAGCTTGGCAGCAGAATTGACACGGGAGGTACCTTTTTAGTCAGTTCTATAGGTGTACACAAAAATCATTTGTTTAAAACCCTGGATTACGAAGTTGATTGAGGTCATTAAGGAACCACCATGCTTGTAGGTTACTTCGTGTGTTCAAACAAACGAAAAGCACGTACTATAATTTATCGAACCGTCGTNcoIPciIXcmIEcoRVKV74TG72EM70SF68DT66LI64DV62HT60KL58LC56IK54PG52LA50EV48LE46LL44EK42TT40RV38DL36SA34CL32NT30QK28EH26MI24NF22LK20AA189397-10200URA39,98010,00010,02010,04010,06010,08010,10010,12010,140
    ACAGGACTAGGATGAGTAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTCGTTTCCTGCAGGTTTTTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGTTTCTTCAACACTACATATGCGTATATATACCAATCTAAGTCTGTGCTCCTTCCTTCTGTCCTGATCCTACTCATCGTCGTGCAAGGAATATACATCGAAAGCTGTACTAAATAGAAGCAAAGGACGTCCAAAAACAAGACACGTCAACCCAATTCTTATGACCCGTTAAAGTACAAAGAAGTTGTGATGTATACGCATATATATGGTTAGATTCAGACACGAGGAAGGAAGNdeISbfIVP16SP14HT12AA10RE8KY6TA4KS2M9397-10200URA3 promoterURA310,16010,18010,20010,22010,24010,26010,28010,30010,320
    GTTCTTCCTTCTGTTCGGAGATTACCGAATCAAAAAAATTTCAAAGAAACCGAAATCAAAAAAAAGAATAAAAAAAAAATGATGAATTGAATTGAAAAGCTGTGGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCAAGAAGGAAGACAAGCCTCTAATGGCTTAGTTTTTTTAAAGTTTCTTTGGCTTTAGTTTTTTTTCTTATTTTTTTTTTACTACTTAACTTAACTTTTCGACACCATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGGGCTGTGGGCGGTTGTGGURA3 promoter10,34010,36010,38010,40010,42010,44010,46010,48010,500
    CGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCT10,52010,54010,56010,58010,600
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10616 bp75008000850090009500100005001000150020002500f1 ori9397-102003790-8343CEN/ARS,SwaI,PmlIAmpR promoterAmpR open reading frame (ORF)NmeAIII,AhdIAmpR terminatororitRNA-Tyr (SUP4),US_gRNA_fwd,PacIHDV-Ribozyme,gRNA_NotI_rev,AjuI,NotICas9 gRNA scaffoldEcoRI,EcoNIAscICYC1 Terminator,PaeR7I,TliI,XhoIT7,T7_promoter,M13-fwd,BsrGI,MluI,Acc65I,KpnIlacZαAloIURA3 terminator,NsiIURA3,StuI,PspOMI,ApaI,NcoI,EcoRVURA3 promoter,PciI,XcmI,SbfI,NdeI

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    10616