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EW489 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8[G2V G4W S6L G7L G10W G11D G13L G17M G19I G22M G23Q S24Q]-FKBP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    cggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcggCMVCMV promoter (with TetOs)20406080100120140160
    ccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttgggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaNcoICspCISnaBICMVCMV promoter (with TetOs)180200220240260280300320340
    ccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtSacIBsaBIEco53kITetR binding sitesTetOTetOCMVCMV promoter (with TetOs)360380400420440460480500520
    gtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgTACGAGTGTAATGAGTGCGGTAAGACCTTCAcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacATGCTCACATTACTCACGCCATTCTGGAAGTPmeISacIAflIIBsaBISgrDIEco53kIHindIIIMY2EC4NE6CG8KT10F667-1326ZNF35 ZF Domains 5-8Te...sTetOCMV promoter (with TetOs)CMV540560580600620640660680700
    CACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTGGGAAGGCTTTTACTCAGAGTGCCAACTTGATCGTGCATCAGAGAAGCCATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGCTTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACACCCTTCCGAAAATGAGTCTCACGGTTGAACTAGCACGTAGTCTCTTCGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACGAAStuICspCIT12RS14SN16LI18VH20QR22IH24TG26EK28PF30AC32ND34CG36KA38FT40QS42AN44LI46VH48QR50SH52TG54EK56PY58EC60KE62CG64KA66FS68CF70667-1326ZNF35 ZF Domains 5-8720740760780800820840860
    CAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTTTAGTCAGCTGTCTTGCTTGATTGTGCATCAGAGAATTCACACGGGCGAACGCCCCTATATGTGCGTAGAGTGCGGTAAATGTTTTGGGCGGAGTTCACATGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAAATCAGTCGACAGAACGAACTAACACGTAGTCTCTTAAGTGTGCCCGCTTGCGGGGATATACACGCATCTCACGCCATTTACAAAACCCGCCTCAAGTGTAStuIBsrGISH72LI74VH76QR78IH80TA82EK84PY86DC88SE90CG92KA94FS96QL98SC100LI102VH104QR106IH108TG110ER112PY114MC116VE118CG120KC122FG124RS126SH128667-1326ZNF35 ZF Domains 5-8ZNF250 ZF Domains 1-4Cano...ker8809009209409609801,0001,0201,040
    CTTTTGCAGCACCAACGTATTCACACAGGCGAGAAACCCTATGTTTGCAGCGTGTGCGGTAAGGCGTTTAGCCAGTCCTCAGTCCTTTCCAAACACCGCCGCATACATACTGGTGAGAAGCCATATGAATGTAATGAATGTGGCAAGGCTTTTAGAGTGAGTTCAGATCTCGCTCGAAAACGTCGTGGTTGCATAAGTGTGTCCGCTCTTTGGGATACAAACGTCGCACACGCCATTCCGCAAATCGGTCAGGAGTCAGGAAAGGTTTGTGGCGGCGTATGTATGACCACTCTTCGGTATACTTACATTACTTACACCGTTCCGAAAATCTCACTCAAGTCTAGAGCGAGLL130QH132QR134IH136TG138EK140PY142VC144SV146CG148KA150FS152QS154SV156LS158KH160RR162IH164TG166EK168PY170EC172NE174CG176KA178FR180VS182SD184LA186667-1326ZNF250 ZF Domains 1-41,0601,0801,1001,1201,1401,1601,1801,2001,220
    AACATCATAAGATCCATACAGGAGAGAAGCCACACGAATGTTTGGAGTGTCGAAAGGCTTTTACTCAGCTCAGTCACCTCATTCAGCATCAGAGAATTCACggcgtaagctggggactactgggctcttgggacagccttggatctggcatgagcataggctctatgcaacagGGTTGTAGTATTCTAGGTATGTCCTCTCTTCGGTGTGCTTACAAACCTCACAGCTTTCCGAAAATGAGTCGAGTCAGTGGAGTAAGTCGTAGTCTCTTAAGTGccgcattcgacccctgatgacccgagaaccctgtcggaacctagaccgtactcgtatccgagatacgttgtcCCPaqCIGGG1V2S3W4G5L6L7G8S9W10D11S12L13G14S15G16M17S18I19G20S21M22Q23Q241327-1398QH188HK190IH192TG194EK196PH198EC200LE202CR204KA206FT208QL210SH212LI214QH216QR218IH220667-1326ZNF250 ZF Domains 1-41,2401,2601,2801,3001,3201,3401,3601,3801,400
    AGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAATTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGTCACGTCCACCTTTGGTAGAGGGGTCCTCTGCCCGCGTGGAAGGGGTTCGCGCCGGTCTGGACGCACCACGTGATGTGGCCCTACGAACTTCTACCTTTCTTTAAACTAAGGAGGGCCCTGTCTTTGTTCGGGAAATTCAAATACGATCCGTTCGTCCTCCACTAGGCTCCGACCAvaIBsgISmaIXmaIBsoBITspMIV2QV4ET6IS8PG10DG12RT14FP16KR18GQ20TC22VV24HY26TG28ML30ED32GK34KF36DS38SR40DR42NK44PF46KF48ML50GK52QE54VI56RG58W1399-1719V2QV4ET6IS8PG10DG12RT14FP16KR18GQ20TC22VV24HY26TG28ML30ED32GK34KF36DS38SR40DR42NK44PF46KF48ML50GK52QE54VI56RG58W1399-1719FKBP1,4201,4401,4601,4801,5001,5201,5401,560
    GAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACTGGAAtaggccgctcgaggagggcccgaacaaaaacCTTCTTCCCCAACGGGTCTACTCACACCCAGTCTCTCGGTTTGACTGATATAGAGGTCTAATACGGATACCACGGTGACCCGTGGGTCCGTAGTAGGGTGGTGTACGGTGAGAGCAGAAGCTACACCTCGAAGATTTTGACCTTatccggcgagctcctcccgggcttgtttttgApaIAvaITliIXhoIBseRIBsoBIPspXIPaeR7IPspOMIEcoO109IE60EG62VA64QM66SV68GQ70RA72KL74TI76SP78DY80AY82GA84TG86HP88GI90IP92PH94AT96LV98FD100VE102LL104KL106E1399-1719E60EG62VA64QM66SV68GQ70RA72KL74TI76SP78DY80AY82GA84TG86HP88GI90IP92PH94AT96LV98FD100VE102LL104KL106E1399-1719*1FKBPc-...e1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattatBclIPmeIBGH polyadenylation sequencec-myc epitopePolyhi...e tag1,7601,7801,8001,8201,8401,8601,8801,9001,920
    aaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttcttttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagaClaISphIBspDIBGH polyadenylation sequenceBGH pA1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttcctccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaagNaeINgoMIV2,1202,1402,1602,1802,2002,2202,2402,260
    cccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcacgggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtgBarIMscIXbaIFRTHygromycin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcgggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccPflFIPshAITth111IHygromycin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctggtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacNcoIPstIPvuIAsiSIDraIIIHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactaPflFIRsrIITth111IHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    gctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggctcgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaAjuIDraIIINmeAIIIHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaSacIIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaagggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttcScaIHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagtcaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaNaeIPfoIPsiINgoMIVSV40 pA3,5203,5403,5603,5803,6003,6203,6403,660
    tgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattaBstZ17ISV40 pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgctcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgcSapIBspQI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctPciIAflIIIpUC ori4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccAlwNIpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcaBspHIpUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    aaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgAhdIBsaIAmp (bla)4,9204,9404,9604,9805,0005,0205,0405,060
    tccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcaaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtAclIFspINmeAIIIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagPvuIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcgggaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagcccAclIBcgIScaIXmnIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    atactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgSspIBspHISgrDIbla promoter5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcMfeIAlwNIBsiWIBpu10Iprimer35,9605,9806,0006,0206,0406,0606,0806,1006,120
    ggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttaccggtctatatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatMluISpeIAflIIICMV promoter (with TetOs)CMV6,1406,1606,1806,2006,220
    • Plasmid
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    Editing disabled on read-only sequence.
    6232 bp45005000550050010001500FKBPTetOCMV667-13261399-17191399-17191327-1398pNK018 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)EKo152.4 (DNA)CMV promoter (with TetOs),EK_CMVFb (DNA),EK_CMVFc (DNA),EK_CMVFd (DNA),SnaBIA,EK_CMVFe (DNA),NcoI,CspCITATA box,TetO,TetR binding sites,CMV-Forward Genewiz (DNA),Eco53kI,+6Kozak,start codon,CMVF1 (DNA),ToFlpF (DNA),EKbF (DNA),+5CspCIZNF35 ZF Domains 5-8,StuIEWo51 (DNA),EWo126 (DNA),BsrGI,StuICanonical ZF LinkerEWo100.2 (DNA),EWo100.3 (DNA)ZNF250 ZF Domains 1-4EWo489.1 (DNA)EWo489.2 (DNA),PaqCI,BsgIFKBP3 (DNA)AvaI,BsoBI,TspMI,XmaI,SmaIXZp017test (DNA)BspHIAhdIBsaINmeAIIIFspI,AclIAmp (bla)PvuIScaI,BcgIAclIXmnIbla promoter,SspI,BspHI,SgrDIprimer3,FlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),EKo279.1 (DNA),EKo272.1 (DNA),+8

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    6232