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XZ024 CMV-TO-bArrestin2-MCP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaCspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgggggagaaacccgggaccagggtcttcaagaagtcgagccctaactgcaagctcaccgtgtacttgggcaagcgggacttcgtagatcacctggacaaagtggaccctgtagattctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtaccccctctttgggccctggtcccagaagttcttcagctcgggattgacgttcgagtggcacatgaacccgttcgccctgaagcatctagtggacctgtttcacctgggacatctPmeISmaIXmaIAflIITspMIHindIIIMG2EK4PG6TR8VF10KK12SS14PN16CK18LT20VY22LG24KR26DF28VD30HL32DK34VD36PV38D760-2730ARRB2CMV promot...th TetOs)homolog720740760780800820840860
    tggcgtggtgcttgtggaccctgactacctgaaggaccgcaaagtgtttgtgaccctcacctgcgccttccgctatggccgtgaagacctggatgtgctgggcttgtccttccgcaaagacctgttcatcgccacctaccaggccttccccccggtgcccaacccaccccggcccaccgcaccacgaacacctgggactgatggacttcctggcgtttcacaaacactgggagtggacgcggaaggcgataccggcacttctggacctacacgacccgaacaggaaggcgtttctggacaagtagcggtggatggtccggaaggggggccacgggttgggtggggccgggStuIBfuAIBspMIPaqCIG40VV42LV44DP46DY48LK50DR52KV54FV56TL58TC60AF62RY64GR66ED68LD70VL72GL74SF76RK78DL80FI82AT84YQ86AF88PP90VP92NP94PR96P760-2730ARRB28809009209409609801,0001,0201,040
    cccacccgcctgcaggaccggctgctgaggaagctgggccagcatgcccaccccttcttcttcaccataccccagaatcttccatgctccgtcacactgcagccaggcccagaggatacaggaaaggcctgcggcgtagactttgagattcgagccttctgtgctaaatcactaggggtgggcggacgtcctggccgacgactccttcgacccggtcgtacgggtggggaagaagaagtggtatggggtcttagaaggtacgaggcagtgtgacgtcggtccgggtctcctatgtcctttccggacgccgcatctgaaactctaagctcggaagacacgatttagtgatcSbfISphIStuIBbvCIBpu10IP98TR100LQ102DR104LL106RK108LG110QH112AH114PF116FF118TI120PQ122NL124PC126SV128TL130QP132GP134ED136TG138KA140CG142VD144FE146IR148AF150CA152KS154L760-2730ARRB21,0601,0801,1001,1201,1401,1601,1801,2001,220
    aagagaaaagccacaaaaggaactctgtgcggctggtgatccgaaaggtgcagttcgccccggagaaacccggcccccagccttcagccgaaaccacacgccacttcctcatgtctgaccggtccctgcacctcgaggcttccctggacaaggagctgtactaccatggggagccttctcttttcggtgttttccttgagacacgccgaccactaggctttccacgtcaagcggggcctctttgggccgggggtcggaagtcggctttggtgtgcggtgaaggagtacagactggccagggacgtggagctccgaagggacctgttcctcgacatgatggtacccctcggAgeIBsgIBsgIEcoNIPspXIE156EK158SH160KR162NS164VR166LV168IR170KV172QF174AP176EK178PG180PQ182PS184AE186TT188RH190FL192MS194DR196SL198HL200EA202SL204DK206EL208YY210HG212EP214760-2730ARRB21,2401,2601,2801,3001,3201,3401,3601,3801,400
    cctcaatgtaaatgtccacgtcaccaacaactccaccaagaccgtcaagaagatcaaagtctctgtgagacagtacgccgacatctgcctcttcagcaccgcccagtacaagtgtcctgtggctcaactcgaacaagatgaccaggtatctcccagctccacattctgtaaggtgggagttacatttacaggtgcagtggttgttgaggtggttctggcagttcttctagtttcagagacactctgtcatgcggctgtagacggagaagtcgtggcgggtcatgttcacaggacaccgagttgagcttgttctactggtccatagagggtcgaggtgtaagacattccacBmgBIBsrGISexAILN216VN218VH220VT222NN224ST226KT228VK230KI232KV234SV236RQ238YA240DI242CL244FS246TA248QY250KC252PV254AQ256LE258QD260DQ262VS264PS266ST268FC270KV272760-2730ARRB21,4201,4401,4601,4801,5001,5201,5401,560
    tacaccataaccccactgctcagcgacaaccgggagaagcggggtctcgccctggatgggaaactcaagcacgaggacaccaacctggcttccagcaccatcgtgaaggagggtgccaacaaggaggtgctgggaatcctggtgtcctacagggtcaaggtgaagctggtggtgtatgtggtattggggtgacgagtcgctgttggccctcttcgccccagagcgggacctaccctttgagttcgtgctcctgtggttggaccgaaggtcgtggtagcacttcctcccacggttgttcctccacgacccttaggaccacaggatgtcccagttccacttcgaccaccacaAleIBlpIBsaIYT274IT276PL278LS280DN282RE284KR286GL288AL290DG292KL294KH296ED298TN300LA302SS304TI306VK308EG310AN312KE314VL316GI318LV320SY322RV324KV326KL328VV330760-2730ARRB21,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctcgaggcggggatgtctctgtggagctgccttttgttcttatgcaccccaagccccacgaccacatccccctccccagaccccagtcagccgctccggagacagatgtccctgtggacaccaacctcattgaatttgataccaactatgccacagatgatgacattgtgtttgagagctccgcccctacagagacacctcgacggaaaacaagaatacgtggggttcggggtgctggtgtagggggaggggtctggggtcagtcggcgaggcctctgtctacagggacacctgtggttggagtaacttaaactatggttgatacggtgtctactactgtaacacaaactSR332GG334DV336SV338EL340PF342VL344MH346PK348PH350DH352IP354LP356RP358QS360AA362PE364TD366VP368VD370TN372LI374EF376DT378NY380AT382DD384DI386VF388E760-2730ARRB2NES1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggactttgcccggcttcggctgaaggggatgaaggatgacgactatgatgatcaactctgcggcGGTGGTAGCGCTATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGcctgaaacgggccgaagccgacttcccctacttcctactgctgatactactagttgagacgccgCCACCATCGCGATACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCAfeIBclID390FA392RL394RL396KG398MK400DD402DY404DD406QL408CG410GG412SA414MA416SN418FT420QF422VL424VD426NG428GT430GD432VT434VA436PS438NF440AN442GI444AE446W760-2730MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPARRB2PCRNEShomolog1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTXcmII448SS450NS452RS454QA456YK458VT460CS462VR464QS466SA468QN470RK472YT474IK476VE478VP480KG482AW484RS486YL488NM490EL492TI494PI496FA498TN500SD502CE504L760-2730MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCP2,1202,1402,1602,1802,2002,2202,2402,260
    TTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAAAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTAgeIAsiSIBstEIII506VK508AM510QG512LL514KD516GN518PI520PS522AI524AA526NS528GI530YG532GG534TG536GG538SM540AS542NF544TQ546FV548LV550DN552GG554TG556DV558TV560AP562SN564760-2730from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPMCP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAABplIBplIBstXIPpuMIBstZ17IFA566NG568IA570EW572IS574SN576SR578SQ580AY582KV584TC586SV588RQ590SS592AQ594NR596KY598TI600KV602EV604PK606GA608WR610SY612LN614ME616LT618IP620IF622760-2730from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTGCGAATAGCGGAATTTACtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcCGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGACGCTTATCGCCTTAAATGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagApaIBseRIPspXIPspOMIH1H2*1AT624NS626DC628EL630IV632KA634MQ636GL638LK640DG642NP644IP646SA648IA650AN652SG654IY656*760-2730from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPc-myc epitope2,6402,6602,6802,7002,7202,7402,7602,7802,800
    atcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagBclIPmeIH3H4H5H62794-2811BGH polyadenylation sequencePo...g2,8202,8402,8602,8802,9002,9202,9402,960
    tattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgClaISphIBspDIPvuIIBGH polyadenylation sequenceBGH pA2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgattcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaKflINaeIPpuMINgoMIV3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgBarIMscIXbaIPflFITth111IHygromycinFRT3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacacagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    ttggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggAsiSIBfuAIBspMIEcoRIRsrIIDraIIIHygromycin3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcaccgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgNdeIPflFIDraIIITth111IHygromycin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggccAjuIHygromycin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    agcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagtcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcSacIIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    cgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactagNaeIPfoIScaINgoMIVHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtagaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatBsmIPsiISV40 pA4,5604,5804,6004,6204,6404,6604,6804,7004,720
    taccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgcatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgBstZ17I4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggSapIBspQIPvuII4,9204,9404,9604,9805,0005,0205,0405,060
    acagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagattgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctaPciIAflIIIpUC ori5,0805,1005,1205,1405,1605,1805,2005,2205,240
    accaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgctpUC ori5,2605,2805,3005,3205,3405,3605,3805,4005,420
    accccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacacttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgaAlwNIpUC ori5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    cggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcaccgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggBspHIAmp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcAhdIBsaIBsrDIAmp (bla)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgAclIFspIBsrDIAmp (bla)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgactagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactAmp (bla)6,3206,3406,3606,3806,4006,4206,4406,460
    gtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagcAclIBcgIScaIXmnIAmp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    atgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggtttacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaaSspIAmp (bla)bla promoter6,6606,6806,7006,7206,7406,7606,7806,8006,820
    attgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtattaacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcataBspHISgrDIbla promoter6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIAlwNIBsiWIAflIIIBpu10I7,0207,0407,0607,0807,1007,1207,140
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7146 bp500055006000650050010001500ARRB2startCMVTetR binding sitesTetO760-2730XZp024.1 (DNA)EKbF (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)EK_CMVFa (DNA),EK_CMVFb (DNA),EK_CMVFc (DNA),EKo272.1 (DNA),SpeI,NdeICMV promoter (with TetOs),A,EK_CMVFd (DNA),EK_CMVFe (DNA),SnaBI,CspCITATA box,TetO,CMV-Forward Genewiz (DNA),Eco53kI,SacI,SgrDI,Eco53kI,+1homolog,Kozak,CMVF1 (DNA),ToFlpF (DNA),XZp025.1 (DNA),PmeI,+2TspMI,XmaI,SmaIBfuAI,BspMI,PaqCIStuISbfI,BbvCI,Bpu10I,SphIStuIARRB2-seq-F1 (DNA)BsgI,BsgI,AgeI,PspXIEcoNIBmgBISexAI,BsrGIBlpI,BsaIAleIARRB2-seq-F2 (DNA)pUC oriAlwNIBspHIAhdIBsaIBsrDIFspI,AclI,BsrDIAmp (bla)ScaIBcgI,AclI,XmnIbla promoter,SspI,BspHIFlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),EKo152.4 (DNA),EKo279.1 (DNA),SgrDI,+6

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    7146

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