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pUC_gapped_wt
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAZraIAatIIBciVIBsaHIEcoO109IpUC19-fseq 20406080100120140160
    TCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGApaLIBaeGIbla genebla signal peptide180200220240260280300320340
    AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAATCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTScaIBsaHIbla geneBcgIXm...I360380400420440460480500520
    AGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGAPvuIbla gene540560580600620640660680700
    GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTCTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGABglIFspIBsrDINmeAIIIbla geneBglI720740760780800820840860
    GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCAAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATAhdIBanIBmrIBpmIBsrDIBsrFIbla genepUC-remBSA-F pUC-remBSA-R Ea...I8809009209409609801,0001,0201,040
    AGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCbl...e1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTATAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATBceAIrep originRNAI transcript1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCACCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTAlwNIApaLIBaeGIBseYIrep origin1,4201,4401,4601,4801,5001,5201,5401,560
    GCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACABciVIrep origin1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGDrdINspIPciIrep originDrd...DI1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCACGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTSapIBsaXIBspQIBsaXI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCCGCTTCATCCAATCTCGAGCATCTTTCATTGATGGAAATTTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGGCGAAGTAGGTTAGAGCTCGTAGAAAGTAACTACCTTTAAABanITliIXhoIPaeR7IHom3-trunc-loc2 homologous arm 3pUC19-rseqCAP...nd2,1202,1402,1602,1802,2002,2202,2402,260
    TTGAGGAGCGCAGTTTTTACCATAGATGTCTTTGTATTTTGACTCTTCCTTACAATGCCTAAACATAGTTGGAAGATATTCTACTTCACGGGTACGTTCCTTTCCATTTTCATCAATATAACGTTCAACAATGTTATTTCCGACTGTTTCAATAGAGATATAAAATTCTTTCATAAACTCCTCGCGTCAAAAATGGTATCTACAGAAACATAAAACTGAGAAGGAATGTTACGGATTTGTATCAACCTTCTATAAGATGAAGTGCCCATGCAAGGAAAGGTAAAAGTAGTTATATTGCAAGTTGTTACAATAAAGGCTGACAAAGTTATCTCTATATTTTAAGAAAGTATBarIBseRIhomologous arm 32,2802,3002,3202,3402,3602,3802,4002,4202,440
    GATATTCCTTAGTTTATAGCCCGAGTTATTAGGCTCTTGATATATTATACTCCAAATAAGGGGCCGAAGCCCCTTGCTTAATTACCAATCGTATATTTAGGAACGAGCTTCCATTCATGTTTTTGTTTAAAAGAAATAACTCGGAAGTTATTAGTTAAATCTTTCATAAAAGTTCCTATAAGGAATCAAATATCGGGCTCAATAATCCGAGAACTATATAATATGAGGTTTATTCCCCGGCTTCGGGGAACGAATTAATGGTTAGCATATAAATCCTTGCTCGAAGGTAAGTACAAAAACAAATTTTCTTTATTGAGCCTTCAATAATCAATTTAGAAAGTATTTTCAAGhomologous arm 32,4602,4802,5002,5202,5402,5602,5802,6002,620
    TTTGACCAGGAACGATTTCAATTAGTCCCCAATCTTCTAATAGCCATGCAATCGAATCACGACGAACTTCATCTTCTTCTGTCATTTCAACTTGACGACCATCCATACGAAGCATTTCTTTAATGGGAAATGAACGATATAGTATAGTCCTTTTTTCTGAAGAATATGACAGGACAAACTGGTCCTTGCTAAAGTTAATCAGGGGTTAGAAGATTATCGGTACGTTAGCTTAGTGCTGCTTGAAGTAGAAGAAGACAGTAAAGTTGAACTGCTGGTAGGTATGCTTCGTAAAGAAATTACCCTTTACTTGCTATATCATATCAGGAAAAAAGACTTCTTATACTGTCCTGBsmFIhomologous arm 3GAP-Trans-clmp-F H3-rev-GAP-clamp2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TGATATAGAACTTTATCTTTATTATTAGCAATTCCCATACGAGTCAAAGTTTCTTTTACTTTCAGAAAATCTTCAGGTTTTTTAAGAGTAATTTCAATCATTTTACCATTCCAATGCTAGTTTTTTGAGTTGTTTCTGTTCTTTTACGTTCTTAGTCACTTCTTTCAAAAAATCAACTATATCTTGAAATAGAAATAATAATCGTTAAGGGTATGCTCAGTTTCAAAGAAAATGAAAGTCTTTTAGAAGTCCAAAAAATTCTCATTAAAGTTAGTAAAATGGTAAGGTTACGATCAAAAAACTCAACAAAGACAAGAAAATGCAAGAATCAGTGAAGAAAGTTTTTTAGTGP62Xm...I2,8202,8402,8602,8802,9002,9202,9402,960
    TCCGTGACTAAACCTTTTAGTTCTTTTAATACTAAAGGAAGTTTTCCATTTTTAGTAAGAATTGATTTATAGTTAATTGCATCATTTGTATTAACTTGATACCGCTTAGCAAGTAACTTAATAATCAATACTTCGGTGGAATCTTCAACCAGTTTTGCCCATTTACCATATCTTTAGGCACTGATTTGGAAAATCAAGAAAATTATGATTTCCTTCAAAAGGTAAAAATCATTCTTAACTAAATATCAATTAACGTAGTAAACATAATTGAACTATGGCGAATCGTTCATTGAATTATTAGTTATGAAGCCACCTTAGAAGTTGGTCAAAACGGGTAAATGGTATAGAAABlpID81E80V79L78K77A76W75K74G73Y72R71K703114-3358GP622,9803,0003,0203,0403,0603,0803,1003,1203,140
    TACCACGAGGAACTGCAGCCATTAGATAATTAAAATGAGCTTCATCACTTAAGCCTGATCCAATTAAATTCATAGCATATACAGCTGGCATACACTCTGGAAATTGTGATAATGCATTTTCAACCATGAATTTTGAATAATCTTTTTGAGCAATAGAGCATTTAGTTTTATTATTATGGTGCTCCTTGACGTCGGTAATCTATTAATTTTACTCGAAGTAGTGAATTCGGACTAGGTTAATTTAAGTATCGTATATGTCGACCGTATGTGAGACCTTTAACACTATTACGTAAAAGTTGGTACTTAAAACTTATTAGAAAAACTCGTTATCTCGTAAATCAAAATAATAANsiIPstIAflIIG69R68P67V66A65A64M63L62Y61N60F59H58A57E56D55S54L53G52S51G50I49L48N47M46A45Y44V43A42P41M40C39E38P37F36Q35S34L33A32N31E30V29M28F27K26S25Y24D23K22Q21A20I19S18C17K16T15K14N13N123114-3358GP623,1603,1803,2003,2203,2403,2603,2803,3003,320
    AATAGCTCCAATTATTTCAAAAAATTCATTTTCTGCTTTTTCTTTAAAAGAATCAGCAGCGGATTGGACAGCTGTCCAATCTTTTGAATACCAAGCAACTTGATGCTCGTTTAATTGAATATCATCTTTAAATAAGCTCATATCACTTCCACTGCATTTCGCATGCTAATTGAATTTATCGAGGTTAATAAAGTTTTTTAAGTAAAAGACGAAAAAGAAATTTTCTTAGTCGTCGCCTAACCTGTCGACAGGTTAGAAAACTTATGGTTCGTTGAACTACGAGCAAATTAACTTATAGTAGAAATTTATTCGAGTATAGTGAAGGTGACGTAAAGCGTACGATTAACTTANspISphIPshAI*320KW318QM316EC314AL312QI3103468-4427I11A10G9I8I7E6F5F4E3N2E13114-3358GP62GP44homo1_f1Ps...I3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GAAAAGATAAGCTAAATGCAATTCAGTATTAGCTGCAATACCATGATACTGATTATTTTCGCCGACAATTTCGTACATACGAATAATACTTTGTGGAGTTACACGTGAATAGATTTCTTCGGCAAGTTTACCCACGAACCACGAATAATCAGCCGCATATTTTGGTGCTAAAGCTCTTTTCTATTCGATTTACGTTAAGTCATAATCGACGTTATGGTACTATGACTAATAAAAGCGGCTGTTAAAGCATGTATGCTTATTATGAAACACCTCAATGTGCACTTATCTAAAGAAGCCGTTCAAATGGGTGCTTGGTGCTTATTAGTCGGCGTATAAAACCACGATTTCGAPmlIBsaAIFL308YA306LH304LE302TN300AA298IG296HY294QN292NE290GV288IE286YM284RI282IS280QP278TV276RS274YI272EE270AL268KG266VF264WS262YD260AA258YK256PA254LA2523468-4427GP44spacer 13,5203,5403,5603,5803,6003,6203,6403,660
    CTGAGTTGTTTAACATCTTTATTTTTGAGAGACTCAAGAACATCATCAATAGCACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTACGAAAATCAGGAAAATTCTTTTTAACCAAAGCTGCTAGACTCAACAAATTGTAGAAATAAAAACTCTCTGAGTTCTTGTAGTAGTTATCGTGGTGCTAGCAATCATTGGTCACTATTTTATGGTCGTAGGTTTTGTGGAAATCTGCTTATTGATAGCTCGAGCGGTTATCAAAATGCTTTTAGTCCTTTTAAGAAAAATTGGTTTCGACGATPvuISacIBanIIEco53kIRL250QK248VD246KN244KL242SE240LV238DD236IA234GR232DN230TV228LS226LI224GA222DL220VG218KS216SY214SD212LE210GI208TK206RF204DP202FN200KK198VL196AA194V3468-4427GP44Mseq-gp44-151-R spacer 2Mseq.G...B2.R Spacer 3BcgI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CAACTTTCATATCAGCTATAGCAATTCCTTCATGCTTGCAGATTTCAGTCAATCGACGAATCATCTGCTTCATCATTTCAATTTTATCTTCATCAGTTGGTTGACCGAATGTAATAACTCGGCAGCGTGACTGAAGCGGTTTAATAATACCATCAATATTATTAGCAGTAATAATGTTGAAAGTATAGTCGATATCGTTAAGGAAGTACGAACGTCTAAAGTCAGTTAGCTGCTTAGTAGACGAAGTAGTAAAGTTAAAATAGAAGTAGTCAACCAACTGGCTTACATTATTGAGCCGTCGCACTGACTTCGCCAAATTATTATGGTAGTTATAATAATCGTCATTATTAHincIINmeAIIIV192KM190DA188IA186IG184EH182KC180IE178TL176RR174IM172QK170MM168EI166KD164ED162TP160QG158FT156IV154RC152RS150QL148PK146II144GD142IN140NA138TI136I3468-4427GP44spacer 4BcgI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    AATACTACAGTTTGAACTATAAGCTTCCATAAAGGAACGAAGATGTCGCTGAGACTCTGCTAACCCTGAACGGTCAAATTCATCAATAACGATTACTTTTTGACGACCATCAAATGAAGCGGCGCTGGCAAAATTAGTCAAAGGACCACGAACGAAATCAATTTTACAATCTGACTTATGATGTCAAACTTGATATTCGAAGGTATTTCCTTGCTTCTACAGCGACTCTGAGACGATTGGGACTTGCCAGTTTAAGTAGTTATTGCTAATGAAAAACTGCTGGTAGTTTACTTCGCCGCGACCGTTTTAATCAGTTTCCTGGTGCTTGCTTTAGTTAAAATGTTAGACTGHindIIII134SC132NS130SY128AE126MF124SR122LH120RQ118SE116AL114GS112RD110FE108DI106VI104VK102QR100GD98FS96AA94SA92FN90TL88PG86RV84FD82IK80CD78S3468-4427GP44spacer12...r) cas9Mseq....2.F Mseq....R Mseq....F 4,0404,0604,0804,1004,1204,1404,1604,1804,200
    CCATTCACAAACATCATATCAGCATTTACATCATGACATAATGCTTTTGCTACAGTTGTTTTACCTGTTCCTGGAGAAGGAGAATGAAGAATAATATGTGGAATCTTACCTTTACTTGTAATAGATTTAAAGGTTTCTTTATCAAAAGCGGGAAGAATACATTCATCGATAGTAGGGTAAGTGTTTGTAGTATAGTCGTAAATGTAGTACTGTATTACGAAAACGATGTCAACAAAATGGACAAGGACCTCTTCCTCTTACTTCTTATTATACACCTTAGAATGGAAATGAACATTATCTAAATTTCCAAAGAAATAGTTTTCGCCCTTCTTATGTAAGTAGCTATCATCBpmIClaIPfoIBspDIEcoNIG76NV74FM72MD70AN68VD66HC64LA62KA60VT58TK56GT54GP52SP50SH48LI46IH44PI42KG40KS38TI36SK34FT32EK30DF28AP26LI24CE22DI20TS183468-4427GP44Ec...IXm...I4,2204,2404,2604,2804,3004,3204,3404,360
    ATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAATCATAATTTCCTCATTCAAGTTTTAGTGTAAATTATAAAGGCCGAAGCCCTCTATTAAAAATCGTGGGTAGAATCAGCTTCAAGAGCTACCACATAATTCGCGTGTTCACCTTCAAATTTAGCAGCATACCTGCTATAAAGACAAGTTCTTACACAAGAAAAAGTAAATGTCATTAGTATTAAAGGAGTAAGTTCAAAATCACATTTAATATTTCCGGCTTCGGGAGATAATTTTTAGCACCCATCTTAGTCGAAGTTCTCGATGGTGTATTAAGCGCACAAGTGGAAGTTTAAATCGTCGTPR16YK14QE12LI10HE8KE6NV4TI2M3468-4427*F228DH226TS224DA222EL220AV218VY216NA214HE212GE210FK208AA2064478-5164GP45GP44homo1_r1Mseq.GP44.B1.F homo2_f1termina...reviewXm...I4,3804,4004,4204,4404,4604,4804,5004,5204,540
    CCTTGTTTACCTTTTGCCCAAAGCAGAAGTTTATAATTTCCTGGTTGCATTTTCATATTTGCCATATTGATAATGAAATTAAATGTATTTTCACCATCATAATCACCAAGAGTCAAAGAATATTTAACCCGGGTCAGAGCAGAATCTTCTACTTTATTAAAACCGTTAATTACGAGGAACAAATGGAAAACGGGTTTCGTCTTCAAATATTAAAGGACCAACGTAAAAGTATAAACGGTATAACTATTACTTTAATTTACATAAAAGTGGTAGTATTAGTGGTTCTCAGTTTCTTATAAATTGGGCCCAGTCTCGTCTTAGAAGATGAAATAATTTTGGCAATTAATGCTSmaIXmaITspMIGQ204KG202KA200WL198LL196KY194NG192PQ190MK188MN186AM184NI182IF180NF178TN176EG174DY172DG170LT168LS166YK164VR162TL160AS158DE156VK154NF152GN150IV148I4478-5164GP45Mseq-GP45-R 4,5604,5804,6004,6204,6404,6604,6804,7004,720
    TTTTACCTTCTTTTACCGTGATAGCAATTGTATCAATTTGCAGACCACGAGATACACGCAACAGCTGTTGAAGGTCTTCAGCTTTAATTTCAGTAACAGCAGATGCTACCGGGAATGGAATTGGTTTATTAGGAGCAACTACTGTACTCGGATCGGCTGCTGGCCAAAAAATTGTAAAATGGAAGAAAATGGCACTATCGTTAACATAGTTAAACGTCTGGTGCTCTATGTGCGTTGTCGACAACTTCCAGAAGTCGAAATTAAAGTCATTGTCGTCTACGATGGCCCTTACCTTAACCAAATAATCCTCGTTGATGACATGAGCCTAGCCGACGACCGGTTTTTTAACABbsIMfeIMscIK146GE144KV142TI140AI138TD136IQ134LG132RS130VR128LL126QQ124LD122EA120KI118ET116VA114SA112VP110FP108IP106KN104PA102VV100TS98PD96AA94PW92FI90T4478-5164GP45Xm...I4,7404,7604,7804,8004,8204,8404,8604,8804,900
    TGAGCGGGCATCAGCAATTTTAATATTTCCATCTTCTGACTGGGAAATTTCTGCATCATCATTAACTAAAGACAGAATACCGAGAAAACCGTTCAAATCGTAAATTGCTACATCAAAATCAATAACGTCAGAAATATTTGCTTCCGCATAAGTTGTACCATTAACTGCGCGAGTCACTCGCCCGTAGTCGTTAAAATTATAAAGGTAGAAGACTGACCCTTTAAAGACGTAGTAGTAATTGATTTCTGTCTTATGGCTCTTTTGGCAAGTTTAGCATTTAACGATGTAGTTTTAGTTATTGCAGTCTTTATAAACGAAGGCGTATTCAACATGGTAATTGACGCGCTCAGBmrIXcmIS88RA86DA84IK82IN80GD78ES76QS74IE72AD70DN68VL66SL64IG62LF60GN58LD56YI54AV52DF50DI48VD46SI44NA42EA40YT38TG36NV34AR32T4478-5164GP454,9204,9404,9604,9805,0005,0205,0405,060
    ATAATAAATTGACCGGATTTAAGCATAATACCAGAGTTAATAGTAGCGAAATTTTTAAGCAGAGCAGTAGTATCTTTAGACAGTTTCATGTAATTTCCTTCAATTCAAATGAGATTTAATTTTATAACTAATTTAATAAAGCAATTAACGATTAAAATCAGCCGCAATTGTTTCCTATTATTTAACTGGCCTAAATTCGTATTATGGTCTCAATTATCATCGCTTTAAAAATTCGTCTCGTCATCATAGAAATCTGTCAAAGTACATTAAAGGAAGTTAAGTTTACTCTAAATTAAAATATTGATTAAATTATTTCGTTAATTGCTAATTTTAGTCGGCGTTAACAAAGGMfeIBsaBIM30IF28QG26SK24LM22IG20SN18IT16AF14NK12LL10AT8TD6KS4LK2M4478-5164GP45Bs...I5,0805,1005,1205,1405,1605,1805,2005,2205,240
    GCAACAATTTGAGCAGCAACAATTAGACGTTCATCTGCGGGGTAATATCAGAAAGAGACAAAACCCATGCTCGACGAATTTTATTTTGGTTTTGTGGATTTGATTCAGAACGCACGTGTTTTGGCTGAATATCTTTTACATCAACAGTATAATTCACAGTAATTTTAGTCATAATCGTTGTTAAACTCGTCGTTGTTAATCTGCAAGTAGACGCCCCATTATAGTCTTTCTCTGTTTTGGGTACGAGCTGCTTAAAATAAAACCAAAACACCTAAACTAAGTCTTGCGTGCACAAAACCGACTTATAGAAAATGTAGTTGTCATATTAAGTGTCATTAAAATCAGTATTAAjuIPmlIBsaAIhomologous arm 45,2605,2805,3005,3205,3405,3605,3805,4005,420
    ACACCTTTAGTCATAATAATCAGTAACAGTCCAAGCTTCATTTCTATTGGACATTATTTTTGTATATTCTGCTTTAAATGCATTCCTAAGCATAGATTCAGTAACTATATGCTCTTCATTAGAAAAATTATTTCTCAGAATATATCGTTTTATTTCAGGAATAGTTAATAGATGCTGTGGAAATCAGTATTATTAGTCATTGTCAGGTTCGAAGTAAAGATAACCTGTAATAAAAACATATAAGACGAAATTTACGTAAGGATTCGTATCTAAGTCATTGATATACGAGAAGTAATCTTTTTAATAAAGAGTCTTATATAGCAAAATAAAGTCCTTATCAATTATCTACGAjuIBsmINsiISapIBspQIBpu10IHindIIIhomologous arm 45,4405,4605,4805,5005,5205,5405,5605,5805,600
    TGTCCAGTTGAATATTCCATGTTTTTCCTCCATAGAGATTATACTCTAATAAATTAAAGCATAATCTCTTATAAATTAAACCATTACAGTAAATCGACCAACTTTCTTCATTTGAAGATGCTGACCATATTCTTGCGGGTCATGGTCTTTATGCGAAATTATAAAAACGTTAGTGACAGGTCAACTTATAAGGTACAAAAAGGAGGTATCTCTAATATGAGATTATTTAATTTCGTATTAGAGAATATTTAATTTGGTAATGTCATTTAGCTGGTTGAAAGAAGTAAACTTCTACGACTGGTATAAGAACGCCCAGTACCAGAAATACGCTTTAATATTTTTGCAATCAChomologous arm 45,6205,6405,6605,6805,7005,7205,7405,760
    TTTTTCATTGAATTTATAATATTAGCTACACCTTTAATACCTTCGGCATCAAATGACCCATCAAACACTTCATCAAGAATTAATGTACTAATACTAACACCAGATACGATAGAAGCAATATCACGCCAAGTAAATAAAAGAGCAATATCGATTCGTGCCTTTTCACCTTCACTAAAAAAAGTAACTTAAATATTATAATCGATGTGGAAATTATGGAAGCCGTAGTTTACTGGGTAGTTTGTGAAGTAGTTCTTAATTACATGATTATGATTGTGGTCTATGCTATCTTCGTTATAGTGCGGTTCATTTATTTTCTCGTTATAGCTAAGCACGGAAAAGTGGAAGTGATTClaIBspDIhomologous arm 45,7805,8005,8205,8405,8605,8805,9005,9205,940
    ATGAAGCATAACTAAAATCTTCACGACCACGGGATTTAATTGTCTCATTAAATTCTTCATCTAATGTAAACACATAATCCGCTTCCATTATTTTAAGATAATGGTTAATCTGCTTATTAAATAATGGAATGTACTTTTTAATAATAGCACCTTTAATACCAGAATCTTTGAGCATTACTTCGTATTGATTTTAGAAGTGCTGGTGCCCTAAATTAACAGAGTAATTTAAGAAGTAGATTACATTTGTGTATTAGGCGAAGGTAATAAAATTCTATTACCAATTAGACGAATAATTTATTACCTTACATGAAAAATTATTATCGTGGAAATTATGGTCTTAGAAACTCGTABtgIhomologous arm 45,9605,9806,0006,0206,0406,0606,0806,1006,120
    ATCAGTCAAAATTCCTCGGTGGTATTTTTCCATTACTAAATTAGTTTTTGTCTTAACAATTTTATCAAGTTCTTCTTGAAGCAGTGCTATTTCATCAGCATGGTCAATAAACTCAGAAGATGCTTTTTCTATAGCCGCTTTAACTTTTTTAGCTTTATCTACTGCTGCGATCAGATAGTCAGTTTTAAGGAGCCACCATAAAAAGGTAATGATTTAATCAAAAACAGAATTGTTAAAATAGTTCAAGAAGAACTTCGTCACGATAAAGTAGTCGTACCAGTTATTTGAGTCTTCTACGAAAAAGATATCGGCGAAATTGAAAAAATCGAAATAGATGACGACGCTAGTCThomologous arm 46,1406,1606,1806,2006,2206,2406,2606,2806,300
    GATTGCTTTTTATTGCGAATATCACCAGAGGTTTTCACCGTCATCACCGAAACGCGCGACTAACGAAAAATAACGCTTATAGTGGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTBsmBIEsp3IHom4-loc1-gib homologous arm 4pUC-bkbone-Kpn1 6,3206,340
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6359 bp450050005500600050010001500GP45rep originBseYI, PspFIGsaIR87EcoP15I4478-5164BauI, BssSI, Bst2BI,pUC19-fseq ,BspHI, CciI, PagI,AatII,ZraI,-35 signal,+18BpuEI,XmnI, Asp700I, MroXI, PdmI,AclI, Psp1406I,DraI,BcgI,TaqII,ScaI,+3bla gene,BtsI,BtsI,PvuI, Ple19I,TaqII,PvuIBsrDI, Bse3DI, BseMI,AclI, Psp1406I,Acc16I, FspI, NsbI,+3EciI,NmeAIII,BglI,GsuI, BpmI,pUC-remBSA-F ,pUC-remBSA-R ,+7BmrI, BmuI,Eam1105I, AhdI, BmeRI, DriI,BmrI,AhdI,BanIDraI,DraIBspHI, CciI, PagI,BpuEI-35 signal,-10 signal,EcoP15I,RNAI transcriptEco57I, AcuI,RNAI promoter (clockwise); GCTACA -10 signal,+1AlwNI, CaiI, PstNI,EcoP15I,AlwNIBpuEIApaLI, Alw44I, VneI,ApaLI,BaeGI,BseYIBauI, BssSI, Bst2BI,PvuII,XmnI, Asp700I, MroXI, PdmI,+4EcoP15I,BalI,BalI, MlsI, MluNI, Mox20I, MscI, Msp20I,+5BmrISspIPsiI, AanIBsaBI, Bse8I, BseJI,MfeI, MunI,GAP2,BsaBI,MfeI,AjuIBsaAI,PmlIHindIII,DraI,HindIII,AjuI,BsmI,NsiI,Bpu10IBspQI,SapIhomologous arm 4ClaI,BspDI,ClaI,BtgIHom4-loc1-gib ,pUC-bkbone-Kpn1 ,BsmBI,Esp3I

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    6359