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pED17x3 - RepA (E93R) SC101 proD SD8 GFP-151-GGGG tetA-orfL kanR
  • Sequence Map
    Editing disabled on read-only sequence.
    tcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtagtctaggaaggcataaatcggtcatacaagagatcacaccaagcaacaaaaacgcactcggtactcttgcttggtaactctagtacgaatgaaacgtacagtgagtttttaaaacggagttttgaccactcgacttaaaaacgtcaatttcgtagcacatcacaaaaagaatcaRepA20406080100120140160
    ccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtggcaatgcatccatccttagactacattaccaacaaccataaaacagtggtaagtaaaaatagaccaacaagagttcaagccaatgctctaggtaaacagatagatcaagttgaaccttttagttgcatagtcagcccgccggagcgaatagttggtggttaaagtataacgacaBpuEIBsaAISnaBIRepA180200220240260280300320340
    aagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatttcacaaatttagaaatgaataaccaaagttttgggtaaccaattcggaaaatttgagtaccatcaataaaagttcgtaattgtacttgaatttaagtagttccgattagagatataaacggaacactcaaaagaaaacacaatcaagaaaattattggtgagtatttaggagtaDraIDraIRepA360380400420440460480500520
    agagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatccgaaagcctttaaccaaaggattcctgtctcataaacaaaagttttctgaattgtacaaggtctaatataaaatacttaaaaaaattgaccttttctattccgttatagagaagtgatttttgattaagattaaaaagcgaactcttgaaccgtatcaaacaggtgaccttttaggctttcggaaattggtttcctaaggacPciIBpuEIAflIIIRepA540560580600620640660680700
    atttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattataaaggtgtcaagagcagtagtcgagagaccaacgaaatcgattatgtggtattcgtaaaagggatgactacaagtagtagactcgcataaccaatattcacttgctatggcaggcaagaaaggaacatcccaaaagttagcaccccaactcatcacggtgtgtcgtattttaatPsiIRepA720740760780800820840860
    gcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattactagtccttttcctttgagttgtgggtatctgcgaaccaaagtacgaggcaattcagtatcgctgattagcgatcaagtaaacgaaacttttgttgattaagtctgtatgtagagttaaccagatccactaaaattagtgatatggttaactctacccgatcagttactattaatgatcaggaaaaggaaactcaacacccatagacMfeIMfeISpeIpSC101RepA8809009209409609801,0001,0201,040
    taaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactaatttaagacgatctggaaacgaccttttgaacatttaagacgatctgggagacatttaaggcgatctggaaacacacaaaaaaaacaaatataagttcaccaatattaaatatcttatttctttcttatttttttctatttttcttatctagggtcgggacacatattgagtgatPsiIBseYIpSC1011,0601,0801,1001,1201,1401,1601,1801,2001,220
    ctttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacgaaatcagtcaaggcgtcataatgttttcctacagcgtttgcgacaaacgaggagatgttttgtctggaattttgggatttccgaattcatcgtgggagcgttcgagcccgtttagcgacttataaggaaaacagaggctggtagtccgtggactcagcgacagaaaaagcactgBcgIMlyIPleISspIAflIIBseRIpSC1011,2401,2601,2801,3001,3201,3401,3601,3801,400
    attcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttctaagtcaagcgacgcgagtgccgagaccgtcacttacccccatttaccgtgatgtccgcggaaaatacctaagtacgttcctttgatgggtattatgttcttttcgggcagtgcccgaagagtcccgcaaaataccgcccagacgatacaccacgatagactgaaaaacgacaagKasINarISfcISfoIBsaHIHaeIIPluTIpSC1011,4201,4401,4601,4801,5001,5201,5401,560
    agcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtcaagaggacatccggtggctgttttggcggatgagagaagattttcagccttcgtcaaggacgggagactaaaaggtcagactggtgaagcctaatagggcactgtccagtaagtctgaccgattacgtgggtcattccgtcgccatagtagttgtccgaatgggcagaatgacagttctcctgtaggccaccgacaaaaccgcctactctcttctaaaagtcggaEciIXmnIAlwNIBstAPIMspA1IEcoP15IpSC101rrnB1 transcri...al terminatorUSER...ion1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaactatgtctaatttagtcttgcgtcttcgccagactattttgtcttaaacggaccgccgtcatcgcgccaccagggtggactggggtacggcttgagtcttcactttgcggcatcgcggctaccatcacaccccagaggggtacgctctcatcccttgacggtccgtagtttatttBsaIAvaIIBsmFIHaeIIrrnB1 transcriptional terminator1,7601,7801,8001,8201,8401,8601,8801,9001,920
    acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgccataaactgccaggcatcaaattaagcagaagtgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagaggactcatcctgtttaggcggccctcgcctaaacttgcaacgcttcgttgccgggcctcccaccgcccgtcctgcgggcggtatttgacggtccgtagtttaattcgtcttcAclIEciIHgaIBsaHIBsrBIrrnB1 transcriptional terminator1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gccatcctgacggatggcctttttgcgtttctacaaactcgctgaggattctagagcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtataatatattcagggagaccacaacggtttccccggtaggactgcctaccggaaaaacgcaaagatgtttgagcgactcctaagatctcgtgtcgattgtggtgcagcagggatagacgacgggatccagatactcaccaacgacctattgaaatgcccgtacgtattccgagcatattatataagtccctctggtgttgccaaagggBcgIBsaISphIStyIXbaIAvrIIBbvCIBmgBIBsmFIHpy99IBsiHKAIEcoP15IrrnB1 transcriptional terminatorproDUSER Linker2,1202,1402,1602,1802,2002,2202,2402,260
    tctacaaataattttgtttaactttatatctgttattttttaaggaggaaaaaaaaatgagcaaaggtgaagagctgtttacgggtgtagtaccgatcttagtggaattagacggcgacgtgaacggtcacaaatttagcgtgcgcggcgaaggcgaaggtgacgctaccaatggagatgtttattaaaacaaattgaaatatagacaataaaaaattcctcctttttttttactcgtttccacttctcgacaaatgcccacatcatggctagaatcaccttaatctgccgctgcacttgccagtgtttaaatcgcacgcgccgcttccgcttccactgcgatggttaccHgaISapIBmgBIBspQIHpy99IMS2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK26FS28VR30GE32GE34GD36AT38NG402332-2781sfGFPUSER ...tionSD82,2802,3002,3202,3402,3602,3802,4002,4202,440
    taaattgaccctgaagtttatttgcacaacaggcaaattacccgttccgtggcccaccttagtgaccaccctgacctatggcgttcagtgcttcagtcgttacccagatcatatgaaacaacacgattttttcaaatcagccatgcctgaaggatatgttcaagagcgtacaatcatttaactgggacttcaaataaacgtgttgtccgtttaatgggcaaggcaccgggtggaatcactggtgggactggataccgcaagtcacgaagtcagcaatgggtctagtatactttgttgtgctaaaaaagtttagtcggtacggacttcctatacaagttctcgcatgttagAleIBtgINdeIKL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI982332-2781sfGFP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agcttcaaggacgatggcacctataaaacgcgtgcggaagtgaaatttgaaggcgacacattagtaaaccgtatcgaactgaaaggtatcgacttcaaagaagacggcaacattttaggccataagctggaatataactttAATTCTCATAACGTgGGGGattacggccgataaatcgaagttcctgctaccgtggatattttgcgcacgccttcactttaaacttccgctgtgtaatcatttggcatagcttgactttccatagctgaagtttcttctgccgttgtaaaatccggtattcgaccttatattgaaaTTAAGAGTATTGCAcCCCCtaatgccggctatttBbsIEaeIEagIMluIAflIIISF100KD102DG104TY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142YN144FN146SH148NV1502332-2781I1T2A3D4K52786-3073sfGFPUSER...ion2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cagaaaaacggtatcaaggcaaatttcaaaattcgccataacgtggaagacggcagcgttcaattagcggatcattatcaacaaaacacgccgattggtgacgggcctgtactgttacctgacaaccactacctgagcacccagtcagtcctgagcaaagatccgaacgaaaaacgtctttttgccatagttccgtttaaagttttaagcggtattgcaccttctgccgtcgcaagttaatcgcctagtaatagttgttttgtgcggctaaccactgcccggacatgacaatggactgttggtgatggactcgtgggtcagtcaggactcgtttctaggcttgctttttgBbsITatIBsiHKAIQ6K7N8G9I10K11A12N13F14K15I16R17H18N19V20E21D22G23S24V25Q26L27A28D29H30Y31Q32Q33N34T35P36I37G38D39G40P41V42L43L44P45D46N47H48Y49L50S51T52Q53S54V55L56S57K58D59P60N61E62K632786-3073sfGFP2,8202,8402,8602,8802,9002,9202,9402,960
    gcgatcacatggttctgttagaattcgtgaccgctgcaggcattactcacggaatggacgaactctacaagggaagccatcaccaccaccatcactgaacttaattaacggcactcctcagccaagtcaaaagcctccgaccggaggcttttgactacatgcccatggcgtttagcgctagtgtaccaagacaatcttaagcactggcgacgtccgtaatgagtgccttacctgcttgagatgttcccttcggtagtggtggtggtagtgacttgaattaattgccgtgaggagtcggttcagttttcggaggctggcctccgaaaactgatgtacgggtaccgcaaatcBtgINcoIPacIPstISfcIStyIBbvCIBseRIEcoRIMspA1IR64D65H66M67V68L69L70E71F72V73T74A75A76G77I78T79H80G81M82D83E84L85Y86K87G88S89H90H91H92H93H94H95*962786-3073sfGFPP14/tonB bidire...nal terminatorUSER ...tionUSE...on2,9803,0003,0203,0403,0603,0803,1003,1203,140
    aaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaactttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttgMlyIPleIKan3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcgaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcAseIHindIIIKan3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcaAjuIBsmIBsmIPvuISmaISspIXmaIAsiSIBsmBIBsrFIEcoNIEsp3ITspMIKan3,5203,5403,5603,5803,6003,6203,6403,660
    aaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgttggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcClaIBspDIKan3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctcgagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggagctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactactatataaaaaNruITliIXhoIBanIIBspHIPspXIPaeR7IKanKanR Promoter3,8603,8803,9003,9203,9403,9603,9804,0004,020
    atcttgtgcaatgtaacatcagagattttgaaggccaaataggccgttagaacacgttacattgtagtctctaaaacttccggtttatccggcaBglISfiIBsrDIKanR PromoterUSER ...tion4,0404,060
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4072 bp30003250350037502505007501000sfGFPRepAKan2786-3073ED0718 (DNA)ED0720 (DNA)

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    4072