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EW265 SFFV-mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagBspHI*W286HK284IL282SA280GI278145-1005Amp (bla)20406080100120140160
    tcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtBglIBsaIBsrDIEA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SR220L145-1005Amp (bla)180200220240260280300320340
    gaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatccttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagFspIBsrDIL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170EN168LE166PE164WR162D145-1005Amp (bla)360380400420440460480500520
    aaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtacttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgPvuIL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104Y145-1005Amp (bla)540560580600620640660680700
    tcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacaBcgIXmnIE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44145-1005Amp (bla)720740760780800820840860
    aacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgttgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacSspIGV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M145-1005Amp (bla)bla promoter8809009209409609801,0001,0201,040
    tctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgcagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgBglIIBspHISgrDIbla promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagctgcagtaacgccaagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaaaccgttcgatcgacgtcattgcggtBmtIMfeINheISFFV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttttgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgttgggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccgcagtttcggccccggcccgaggccaagaaaacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcaacccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggcgtcaaagccggggccgggctccggttcAloISmaIXmaIEcoRVTspMISFFV1,4201,4401,4601,4801,5001,5201,5401,560
    aacagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttctgcttcccgagctctataaaagagctcattgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagacgaagggctcgagatattttctcgagtSacISacIAflIIBbvCIBpu10IBssHIIEco53kIEco53kISFFV1,5801,6001,6201,6401,6601,6801,7001,7201,740
    caacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCAscIMscISmaIXmaITspMIBssHIIHindIIIM1VS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG24SV26NG28HE30FE32IE34GE36G1816-2523mCherrySFFV1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGATGAACTTCGACAGGAAGGGGCTCCCGAAGStuIE38GR40PY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD82IP84DY86LK88LS90FP92EG94F1816-2523mCherry1,9401,9601,9802,0002,0202,0402,0602,0802,100
    AAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGGAGGCTCGCCTAloIBbsISbfIStuIPflMIK96WE98RV100MN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM140QK142KT144MG146WE148AS150SE152R1816-2523mCherry2,1202,1402,1602,1802,2002,2202,2402,260
    TGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTAACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATBsgIKasIKasINarINarISfoISfoIBbvCIPluTIPluTIBpu10IM154YP156ED158GA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198VN200IK202LD204IT206SH208NE210DY2121816-2523mCherry2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGACGTCGAGTCCAACCCCGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCTGCAGCTCAGGTTGGGGMluIXcmIBsrGISgrAIAflIIID1A2L3E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P312533-2631TI214VE216QY218ER220AE222GR224HS226TG228GM230DE232LY234KS2361816-25235' StopGo (2A; Loughran 2017)mCherry2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAACGTACTGTTGTATAGTACGTCTCAGACTGTTGTATAGTCTGTCTGGTATGTTGTATACGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTGCATGACAACATATCATGCAGAGTCTGACAACATATCAGACAGACCATACAACATATGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCAgeIBaeIKpnISpeIBamHISnaBIAcc65IBstZ17IS1V2*3V4G5G6I7V8T9Y10C11C12I13V14R15L16R17L18L19Y20S21L22S23G24M25L26Y27T28Y29H30S31I32P33P34T35Q36T372644-2754G32P33G1T2G1S2E1A2R3H4K5Q6K7I8V9A10P112767-2865T1G2T1S2ADAR substrate3xPUF 9R-tar3' StopGo (2A; Loughran 2017)5' stem3' stem2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAACTTTTTACGGGCGTTGTTCCGATCCTCGTCGAATTGGACGGGGACGTTAATGGGCATAAATTCTCAGTTAGTGGTGAGGGTGAGGGATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCACAGAGTTTCCCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGGAGCAGCTTAACCTGCCCCTGCAATTACCCGTATTTAAGAGTCAATCACCACTCCCACTCCCPfoITliIXhoIBfuAIBspMIPaqCIPspXIPaeR7IVS2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK26FS28VS30GE32GE34G2872-3585V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P332767-2865S1S2GFP3' StopGo (2A; Loughran 2017)2,8202,8402,8602,8802,9002,9202,9402,960
    GGACGCGACATATGGGAAACTTACGCTGAAGTTCATATGTACCACCGGAAAATTGCCAGTGCCGTGGCCTACTCTCGTCACCACCCTGACATATGGTGTTCAGTGTTTTAGCCGGTATCCAGACCATATGAAACAGCACGATTTTTTTAAATCCGCTATGCCTGAAGGTTACGTACCTGCGCTGTATACCCTTTGAATGCGACTTCAAGTATACATGGTGGCCTTTTAACGGTCACGGCACCGGATGAGAGCAGTGGTGGGACTGTATACCACAAGTCACAAAATCGGCCATAGGTCTGGTATACTTTGTCGTGCTAAAAAAATTTAGGCGATACGGACTTCCAATGCATBarIBstXISnaBID36AT38YG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF84KS86AM88PE90GY92V2872-3585GFP2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CAGGAGCGAACGATATTTTTTAAAGACGACGGAAACTACAAAACTAGAGCCGAGGTAAAATTCGAGGGTGACACTCTCGTGAACCGGATCGAACTGAAGGGCATAGATTTTAAGGAGGATGGCAATATACTGGGCCACAAGCTCGAATACAACTATAATTCTCATAATGTCTATAGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTTTGATGTTTTGATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAGCACTTGGCCTAGCTTGACTTCCCGTATCTAAAATTCCTCCTACCGTTATATGACCCGGTGTTCGAGCTTATGTTGATATTAAGAGTATTACAGATATQ94ER96TI98FF100KD102DG104NY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142YN144YN146SH148NV150Y2872-3585GFP3,1603,1803,2003,2203,2403,2603,2803,3003,320
    TCATGGCGGACAAACAGAAAAACGGCATCAAGGTAAACTTTAAAATCCGACACAATATTGAGGACGGCTCTGTCCAGCTTGCGGACCATTACCAACAGAACACGCCGATAGGGGATGGTCCAGTACTTCTCCCTGATAATCACTATTTGTCTACTCAGTCAGCTCTTAGCAAGGAAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCATTTGAAATTTTAGGCTGTGTTATAACTCCTGCCGAGACAGGTCGAACGCCTGGTAATGGTTGTCTTGTGCGGCTATCCCCTACCAGGTCATGAAGAGGGACTATTAGTGATAAACAGATGAGTCAGTCGAGAATCGTTCCTSspII152MA154DK156QK158NG160IK162VN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY200LS202TQ204SA206LS208KD2102872-3585GFP3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CCCAAACGAAAAACGGGATCACATGGTCCTTCTTGAGTTCGTCACTGCGGCTGGGATAACACTCGGGATGGACGAACTGTATAAGtaggccggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaaccGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAACTCAAGCAGTGACGCCGACCCTATTGTGAGCCCTACCTGCTTGACATATTCatccggcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttggApaIPmeIPspOMIPN212EK214RD216HM218VL220LE222FV224TA226AG228IT230LG232MD234EL236YK2382872-3585*1GFPc-myc epitopePolyhi...e tag3,5203,5403,5603,5803,6003,6203,6403,660
    cgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcgcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgBclIBtgZIBGH polyadenylation sequence3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcBbsIClaINspISphIBglIIBspDIBGH polyadenylation sequenceBGH pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctgNaeINgoMIV4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctgggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagaBarIMscIXbaIPshAIHygromycinFRT4,2204,2404,2604,2804,3004,3204,3404,360
    cggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggaEcoRIHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactctggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgaPvuIAsiSIBfuAIBspMIRsrIIBstAPIDraIIIHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    acatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacBstAPIDraIIIHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaAjuIBtgZISacIIHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    ccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggcggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgHygromycin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgcacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgNaeIPfoINgoMIVHygromycin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcBsmIPsiIBstZ17ISV40 pA5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcggaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagc5,6205,6405,6605,6805,7005,7205,7405,760
    tgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcagacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcSapIBspQI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcNspIPciIAflIIIpUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagapUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggcgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgcccpUC ori6,4806,5006,5206,5406,5606,5806,6006,620
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6632 bp450050005500600050010001500SFFVg -> a145-1005EKo152.2 (DNA)EKo412.2 (DNA)EKo271.3 (DNA)EKo309.1 (DNA)EKo326.2 (DNA)Cit_1 (DNA)pNSK019 (DNA)EKo260.2 (DNA)EKo387.3 (DNA)EKo409.1 (DNA)BGHR Genewiz (DNA)EKo326.3 (DNA)EKo152.1 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)BspHIBsaI,BsrDI,BglIFspI,BsrDIAmp (bla)PvuIBcgIXmnISspIbla promoter,BspHISgrDI,BglIIFlpInF1 (DNA)FlpInF (DNA)EKo285.1 (DNA),EWo118.11 (DNA),MfeIGRo003.3 (DNA)GRo003.4 (DNA),EWo118.12 (DNA),NheI,BmtIEcoRVTspMI,XmaI,SmaIAloI,BbvCI,Bpu10IAflIIAjuISacIIHyg_F (DNA)PfoINgoMIV,NaeISV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiISV40 pA,BsmIBstZ17IM13R Genewiz (DNA)BspQI,SapIpUCori5F (DNA)AflIIIPciI,NspIpUC ori

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    6632