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pGS-21a his6-GST-WTPhi29DNAP
  • Sequence Map
    Editing disabled on read-only sequence.
    taatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatatacatatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaaattatgctgagtgatatccccttaacactcgcctattgttaaggggagatctttattaaaacaaattgaaattcttcctctatatgtatacaggggatatgatccaataaccttttaattcccggaacacgttgggtgagctgaagaaaaccttatagaacttctttttatacttBarINdeISapIXbaIBspQIEcoNIMS2PI4LG6YW8KI10KG12LV14QP16TR18LL20LE22YL24EE26KY28E89-2572GSTGST-DNAPol-6xHislac operatorefficien..., 1989)T7T7 promoter20406080100120140160
    gagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagctcgtaaacatactcgcgctacttccactatttaccgctttgtttttcaaacttaacccaaacctcaaagggttagaaggaataatataactaccactacaatttaattgtgtcagataccggtagtatgcaatatatcgactgttcgtgttgtacaacccaccaacaggttttcMscIPciIE30HL32YE34RD36EG38DK40WR42NK44KF46EL48GL50EF52PN54LP56YY58ID60GD62VK64LT66QS68MA70II72RY74IA76DK78HN80ML82GG84CP86K89-2572GSTGST-DNAPol-6xHis180200220240260280300320340
    agcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcgcacgtctctaaagttacgaacttcctcgccaaaacctataatctatgccacaaagctcttaacgtatatcatttctgaaactttgagagtttcaactaaaagaatcgttcgatggactttacgacttttacaagcttctagcaaatacagtattttgtataaatttaccactBclISwaIBstBIE88RA90EI92SM94LE96GA98VL100DI102RY104GV106SR108IA110YS112KD114FE116TL118KV120DF122LS124KL126PE128ML130KM132FE134DR136LC138HK140TY142LN144GD14689-2572GSTGST-DNAPol-6xHis360380400420440460480500520
    tcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagagtacattgggtaggactgaagtacaacatactgcgagaactacaacaaaatatgtacctgggttacacggacctacgcaagggttttaatcaaacaaaattttttgcataacttcgatagggtgtttaactattcatgaactttaggtcgttcatatatcgtaccggaaacgtcScaIHV148TH150PD152FM154LY156DA158LD160VV162LY164MD166PM168CL170DA172FP174KL176VC178FK180KR182IE184AI186PQ188ID190KY192LK194SS196KY198IA200WP202LQ20489-2572GSTGST-DNAPol-6xHis540560580600620640660680700
    ggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctgggccacacaggccatagatctggtaccgacgacgacgacaaggccatggctgatatcggatccatgccgcgtaaaatgtattcctgtgattttgaaacgacgacgaaagttgaagattgccgtgccgaccgttcggtgcaaaccaccaccgctggtaggaggttttagcctagacccggtgtgtccggtatctagaccatggctgctgctgctgttccggtaccgactatagcctaggtacggcgcattttacataaggacactaaaactttgctgctgctttcaacttctaacggcacKpnINcoISfiIStyIBamHIBglIIAcc65IGW206QA208TF210GG212GD214HP216PK218SD220LG222HT224GH226RS228GT230DD232DD234KA236MA238DI240GS242MP244RK246MY248SC250DF252ET254TT256KV258ED260CR26289-2572GST-DNAPol-6xHisGSTpGEX 5'ente...ite720740760780800820840860
    tgtgggcgtatggttatatgaacatcgaagaccattcagaatataaaattggcaactcgctggatgaatttatggcgtgggtgctgaaagttcaggccgacctgtacttccacaatctgaaatttgatggtgcgttcattatcaactggctggaacgtaatggctttaaatggagacacccgcataccaatatacttgtagcttctggtaagtcttatattttaaccgttgagcgacctacttaaataccgcacccacgactttcaagtccggctggacatgaaggtgttagactttaaactaccacgcaagtaatagttgaccgaccttgcattaccgaaatttacctcVW264AY266GY268MN270IE272DH274SE276YK278IG280NS282LD284EF286MA288WV290LK292VQ294AD296LY298FH300NL302KF304DG306AF308II310NW312LE314RN316GF318KW320S89-2572GST-DNAPol-6xHis8809009209409609801,0001,0201,040
    cgccgatggtctgccgaacacctataatacgattatctctcgtatgggccaatggtatatgattgatatctgcctgggctacaaaggtaaacgcaaaattcataccgtgatctatgacagcctgaaaaaactgccgtttccggtgaagaaaattgcgaaagatttcaaactgaccgcggctaccagacggcttgtggatattatgctaatagagagcatacccggttaccatatactaactatagacggacccgatgtttccatttgcgttttaagtatggcactagatactgtcggacttttttgacggcaaaggccacttcttttaacgctttctaaagtttgactggA322DG324LP326NT328YN330TI332IS334RM336GQ338WY340MI342DI344CL346GY348KG350KR352KI354HT356VI358YD360SL362KK364LP366FP368VK370KI372AK374DF376KL378T89-2572GST-DNAPol-6xHis1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gtcctgaaaggcgatattgactatcacaaagaacgtccggttggttacaaaatcacgccggaagaatatgcgtacattaaaaacgatatccagattatcgcagaagctctgctgattcagtttaaacaaggcctggatcgcatgaccgccggcagtgactccctgaaaggtttcacaggactttccgctataactgatagtgtttcttgcaggccaaccaatgttttagtgcggccttcttatacgcatgtaatttttgctataggtctaatagcgtcttcgagacgactaagtcaaatttgttccggacctagcgtactggcggccgtcactgagggactttccaaagtPmeIStuIV380LK382GD384ID386YH388KE390RP392VG394YK396IT398PE400EY402AY404IK406ND408IQ410II412AE414AL416LI418QF420KQ422GL424DR426MT428AG430SD432SL434KG436F89-2572GST-DNAPol-6xHis1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aagatatcatcaccacgaaaaaattcaaaaaagtgttcccgaccctgagcctgggtctggataaagaagttcgttatgcataccgcggcggttttacgtggctgaacgaccgtttcaaagaaaaagaaattggcgagggtatggtctttgatgtgaatagtctgtatccggctcattctatagtagtggtgcttttttaagttttttcacaagggctgggactcggacccagacctatttcttcaagcaatacgtatggcgccgccaaaatgcaccgacttgctggcaaagtttctttttctttaaccgctcccataccagaaactacacttatcagacataggccgagtNsiISacIIBpu10IK438DI440IT442TK444KF446KK448VF450PT452LS454LG456LD458KE460VR462YA464YR466GG468FT470WL472ND474RF476KE478KE480IG482EG484MV486FD488VN490SL492YP494AQ49689-2572GST-DNAPol-6xHis1,4201,4401,4601,4801,5001,5201,5401,560
    gatgtactcccgcctgctgccgtatggcgaaccgatcgttttcgagggtaaatatgtctgggatgaagactacccgctgcatattcagcacatccgttgtgaatttgaactgaaagaaggctatattccgaccattcaaatcaaacgtagccgcttctataagggtaacgaatacctacatgagggcggacgacggcataccgcttggctagcaaaagctcccatttatacagaccctacttctgatgggcgacgtataagtcgtgtaggcaacacttaaacttgactttcttccgatataaggctggtaagtttagtttgcatcggcgaagatattcccattgcttatgPvuIMY498SR500LL502PY504GE506PI508VF510EG512KY514VW516DE518DY520PL522HI524QH526IR528CE530FE532LK534EG536YI538PT540IQ542IK544RS546RF548YK550GN552EY55489-2572GST-DNAPol-6xHis1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctgaaaagctctggcggtgaaatcgcagacctgtggctgagtaacgtcgatctggaactgatgaaagaacattacgatctgtacaacgttgaatacatctccggcctgaaatttaaagccaccacgggtctgtttaaagatttcattgacaaatggacctacatcaaaaccacgtgacttttcgagaccgccactttagcgtctggacaccgactcattgcagctagaccttgactactttcttgtaatgctagacatgttgcaacttatgtagaggccggactttaaatttcggtggtgcccagacaaatttctaaagtaactgtttacctggatgtagttttggtgcaBmgBIBsrGILK556SS558GG560EI562AD564LW566LS568NV570DL572EL574MK576EH578YD580LY582NV584EY586IS588GL590KF592KA594TT596GL598FK600DF602ID604KW606TY608IK610TT61289-2572GST-DNAPol-6xHis1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctgaaggtgcaatcaaacagctggctaaactgatgctgaacagcctgtatggcaaatttgcatctaatccggatgttaccggtaaagtcccgtacctgaaagaaaatggcgctctgggttttcgcctgggcgaagaagaaaccaaagatccggtgtatacgccgatgggtgttttgacttccacgttagtttgtcgaccgatttgactacgacttgtcggacataccgtttaaacgtagattaggcctacaatggccatttcagggcatggactttcttttaccgcgagacccaaaagcggacccgcttcttctttggtttctaggccacatatgcggctacccacaaaaAccIAgeIBstZ17ISE614GA616IK618QL620AK622LM624LN626SL628YG630KF632AS634NP636DV638TG640KV642PY644LK646EN648GA650LG652FR654LG656EE658ET660KD662PV664YT666PM668GV670F89-2572GST-DNAPol-6xHis1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cattaccgcgtgggcacgttacaccacgatcacggcagcacaagcgtgctatgaccgcattatctactgtgataccgactcaattcatctgaccggcacggaaatcccggatgtgattaaagatatcgttgacccgaaaaaactgggttattgggcacacgaatcgacctttaaagtaatggcgcacccgtgcaatgtggtgctagtgccgtcgtgttcgcacgatactggcgtaatagatgacactatggctgagttaagtagactggccgtgcctttagggcctacactaatttctatagcaactgggcttttttgacccaataacccgtgtgcttagctggaaatttAleIHincIII672TA674WA676RY678TT680IT682AA684QA686CY688DR690II692YC694DT696DS698IH700LT702GT704EI706PD708VI710KD712IV714DP716KK718LG720YW722AH724ES726TF728K89-2572GST-DNAPol-6xHis2,1202,1402,1602,1802,2002,2202,2402,260
    cgtgctaaatacctgcgccagaaaacgtacatccaagacatctacatgaaagaagtcgatggcaaactggtggaaggttcaccggatgactataccgacattaaattttcggtgaaatgcgccggcatgaccgataaaatcaaaaaagaagtcacgttcgaaaatttcaaagtgggcacgatttatggacgcggtcttttgcatgtaggttctgtagatgtactttcttcagctaccgtttgaccaccttccaagtggcctactgatatggctgtaatttaaaagccactttacgcggccgtactggctattttagttttttcttcagtgcaagcttttaaagtttcaccBfuAIBspMIBstBIR730AK732YL734RQ736KT738YI740QD742IY744MK746EV748DG750KL752VE754GS756PD758DY760TD762IK764FS766VK768CA770GM772TD774KI776KK778EV780TF782EN784FK786V89-2572GST-DNAPol-6xHis2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gtttcagtcgcaaaatgaaaccgaaaccggtgcaagttccgggcggcgttgtcctggtggatgataccttcacgattaaacagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgccaaagtcagcgttttactttggctttggccacgttcaaggcccgccgcaacaggaccacctactatggaagtgctaatttgtcgaacgccggcgtgagctcgtggtggtggtggtggtgactctaggccgacgattgtttcgggctttccttcgactcaaccgacgacggtggcgAgeIAvaIEagINotITliIXhoIBsoBIPspXIPaeR7IG788FS790RK792MK794PK796PV798QV800PG802GV804VL806VD808DT810FT812IK814QL816AA818AL820EH822HH824HH826H*82889-2572GST-DNAPol-6xHis6xHis2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttcctccttgatataggcctaaccgcttaccctgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcBlpIStyIf1 oriT7 terminatorT7 Term2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgDraIIIf1 oriF1ori-R2,8202,8402,8602,8802,9002,9202,9402,960
    ggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaaAloIPsiIBsaXIf1 oriF1ori-F2,9803,0003,0203,0403,0603,0803,1003,1203,140
    aacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgttgttttataattgcgaatgttaaatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcSspISspICspCIAmpR promoterf1 oriAmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggtBssSIBssSαIAmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    atgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgtactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacScaIAmpRAmp-R3,5203,5403,5603,5803,6003,6203,6403,660
    ccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacgPvuIAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    agcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcttcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagaFspIPstINmeAIIIAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctAhdIBsaIAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ttgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtori4,2204,2404,2604,2804,3004,3204,3404,360
    aacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgaori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtggacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcacAlwNIori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctctcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagBssSIBssSαIoripBR322ori-F4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcPciIori4,9204,9404,9604,9805,0005,0205,0405,060
    cgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcaatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcggcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgttaccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcAccISapIBspQIPflFIBstZ17ITth111IbompRS-markerL44405,0805,1005,1205,1405,1605,1805,2005,2205,240
    ccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgcBsmBIEsp3IroppGEX 3'5,2605,2805,3005,3205,3405,3605,3805,4005,420
    attcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgtaagtgtctacagacggacaagtaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgactacggaggcacattccccctaaagacaagtacccccattactatggctactttgcrop5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcattctctcctacgagtgctatgcccaatgactactacttgtacgggccaatgaccttgcaacactcccatttgttgaccgccatacctacgccgccctggtctctttttagtgagtcccagttacggtcgcgaagcaattatgtctacatccacaaggtgtcccatcggtcgtcgta5,6205,6405,6605,6805,7005,7205,7405,760
    cctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggacgctacgtctaggccttgtattaccacgtcccgcgactgaaggcgcaaaggtctgaaatgctttgtgcctttggcttctggtaagtacaacaacgagtccagcgtctgcaaaacgtcgtcgtcagcgaagtgcaagcgagcgcatagccactaagtaagacgattggtcattBpu10I5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccccgttggggcggtcggatcggcccaggagttgctgtcctcgtgctagtacgcgtgggcaccccggcggtacggccgctattaccggacgaagagcggctttgcaaaccaccgccctggtcactgcttccgaactcgctcccgcacgttctaaggcttatggcgttcgctgtccggFspIFspAIPpuMI5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtctagtagcagcgcgaggtcgctttcgccaggagcggcttttactgggtctcgcgacggccgtggacaggatgctcaacgtactatttcttctgtcagtattcacgccgctgctatcagtacggggcgcgggtggccttcctcgactgacccaacttccgagagttcccgtagccaPshAI6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacggctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgcBsaXIBsmBIEsp3IlacI6,3206,3406,3606,3806,4006,4206,4406,460
    ggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcaccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcatagggtgatggctctacaggcgtggttgcgcgtHpaIHincIIlacI6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatacgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactctatBssHIIlacI6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacaaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaactacccacagaccagtctctgtagttctttattgApaIBstEIIPspOMINmeAIIIlacI6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccBclIMluIlacI7,0207,0407,0607,0807,1007,1207,1407,160
    cgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaclacI7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgcgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatattgcaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaaaacgcggtaagctaccacaggccctagagctgcPflMIBstAPIlacIlacI promoterLacI-R7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcggcggcgttccttaccacgtacgttcctctaccgcgggttgtcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgagtactcgggcttcaccgctSphIEcoNI7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatcgatctcgatcccgcgaaatcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagctctagctagagctagggcgctttaClaIBspDISgrAIpBRrevBam7,7207,7407,7607,7807,8007,820
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7824 bp5500600065007000750050010001500GST-DNAPol-6xHislacIenterokinase sitepGEX 5'89-2572Backbone_pGS21a_rev (DNA)Backbone_pGS21a_fwd (DNA)T7 promoter,T7,lac operator,+9GST,BstBI,SwaI,BclIScaISfiIBglIIAcc65IKpnI,NcoI,StyI,BamHIPmeI,StuI,Bpu10INsiI,SacIIPvuIBsrGIBmgBIBpu10IPpuMIFspAI,FspIPshAIBsaXI,BsmBI,Esp3IHincII,HpaIBssHIIPspOMI,ApaI,NmeAIII,BstEIIBclI,MluILacI-R,BstAPIlacI promoter,pBRrevBam,PflMI,EcoNI,SphI,SgrAI,BspDI,ClaI

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    7824