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pTS056
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    tgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacactgaatcatttcgtgtagattttgaaaatcgcaataatgcattttttagaacggtcgaaaggggaagatttcccgttttcactcataccacggatagattgtagagttaccgattccgcagctcgtttcgggcgaataaaaaatgtacggttatgttacatccgacgagatgtgFauIBsaAIBtgZIEcoNISnaBIBpu10ITetR20406080100120140160
    ctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctagacatttgtattacctccttatttcctctagtaAAAGTTAAACAAAATTATTTGTAGAGGGAAACCGTTGTGGTCTCgatcgaagacccgctcaaatgcccaacaatttggaagctaaggctggagtaattcgtcgagattacgcgacaattagtgaaatgaaaatagattagatctgtaaacataatggaggaataaaggagatcatTTTCAATTTGTTTTAATAAACATCTCCCTTTGGCAACACCAGAGproB-RBS expression element (Rogers 2015)RBS for... 2015)TetR180200220240260280300320340
    CCTGAATATatattaCGAGCCTTATGCATGCCcgtaaaGTTATCCAGCAACCACTCATAGACCTAGGGCAGCAGATAGGGACGACGTGGTGTTAGCTGTGctctagaaTCTGCGGCCGCTCACAtccctatcagtgatagagattgacatccctatcagtgatagagatactgagGGACTTATAtataatGCTCGGAATACGTACGGgcatttCAATAGGTCGTTGGTGAGTATCTGGATCCCGTCGTCTATCCCTGCTGCACCACAATCGACACgagatcttAGACGCCGGCGAGTGTagggatagtcactatctctaactgtagggatagtcactatctctatgactcBcgIBsaIEagINotINsiISphIAvrIIBmgBIBsaBIBsmFIproB-RBS expression element (Rogers 2015)proB-promoter (Davis 2010)PLtetO-1 (Lutz Bujard 1997)360380400420440460480500520
    cacatcagcaggacgcactgaccaagaattcaaaaGATCTAAAGAGGAGAAAGGATCTgtgttttcttattactttcaaggtcttgcacttggggcggctatgatcctaccgctcggtccacaaaatgcttttgtgatgaatcagggcatacgtcgtcagtaccacattatgattgtgtagtcgtcctgcgtgactggttcttaagttttCTAGATTTCTCCTCTTTCCTAGAcacaaaagaataatgaaagttccagaacgtgaaccccgccgatactaggatggcgagccaggtgttttacgaaaacactacttagtcccgtatgcagcagtcatggtgtaatactaaApoIBglIIEcoRIargO CDS (arginine transporter)Strong RBS (El...Leibler 2000)PLtetO-1...d 1997)540560580600620640660680700
    gccttactttgtgctatcagcgatttggtcctgatttgcgccgggatttttggtggcagcgcgttattgatgcagtcgccgtggttgctggcgctggtcacctggggcggcgtagccttcttgctgtggtatggttttggcgcttttaaaacagcaatgagcagtaatattgagtcggaatgaaacacgatagtcgctaaaccaggactaaacgcggccctaaaaaccaccgtcgcgcaataactacgtcagcggcaccaacgaccgcgaccagtggaccccgccgcatcggaagaacgacaccataccaaaaccgcgaaaattttgtcgttactcgtcattataactcaBtgIDraIBstEIIargO CDS (arginine transporter)720740760780800820840860
    tagccagcgccgaagtcatgaagcaaggcagatggaaaattatcgccaccatgttggcagtgacctggctgaatccgcatgtttacctggatacttttgttgtactgggcagccttggcgggcaacttgatgtggaaccaaaacgctggtttgcactcgggacaattagcgcctcatcggtcgcggcttcagtacttcgttccgtctaccttttaatagcggtggtacaaccgtcactggaccgacttaggcgtacaaatggacctatgaaaacaacatgacccgtcggaaccgcccgttgaactacaccttggttttgcgaccaaacgtgagccctgttaatcgcggagAvaIBaeIFauIBciVIBsmFIBsoBIBspHIBstXIBstXIargO CDS (arginine transporter)8809009209409609801,0001,0201,040
    tttcctgtggttctttggtctggctcttctcgcagcctggctggcaccgcgtctgcgcacggcaaaagcacagcgcattatcaatctggttgtgggatgtgttatgtggtttattgccttgcagctggcgagagacggtattgctcatgcacaagccttgttcagttagCtcgagaaaggacaccaagaaaccagaccgagaagagcgtcggaccgaccgtggcgcagacgcgtgccgttttcgtgtcgcgtaatagttagaccaacaccctacacaatacaccaaataacggaacgtcgaccgctctctgccataacgagtacgtgttcggaacaagtcaatcGagctcAvaIBglIBpmIFspISapITliIXhoIBsmBIBsoBIBspQIEsp3IPspXIPvuIIPaeR7IargO CDS (arginine transporter)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacctagggatatattccgcttcctcgctcactgactcgattcctagaggtccgtagtttattttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagagatgatctcagtgtgaccgagtggaagcccacccggaaagacgcaaatatggatccctatataaggcgaaggagcgagtgactgagcPsrIAvrIIrrnB T1 terminatorT7Te terminator1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggatgcgagccagcaagctgacgccgctcgcctttaccgaatgcttgccccgcctctaaaggaccttctacggtccttctatgaattgtcccttcactctcccggcgccgtttcggcaaaaaggtatccgaggcggggggactgttcgtagtgctttagactgcgagtttagtcacBtgIPfoISacIIp15A ori1,4201,4401,4601,4801,5001,5201,5401,560
    gtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggacaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccgccgagggagcacgcgagaggacaaggacggaaagccaaatggccacagtaaggcgacaataccggcgcaaacagagtaaggtgcggactgtgagtcaaggcccatccgtcaagcgaggttcgacctAgeIBssSIBssSαIp15A ori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaaggacatacgtgcttggggggcaagtcaggctggcgacgcggaataggccattgatagcagaactcaggttgggcctttctgtacgttttcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatcagaacttcagtacgcggccaattccgatttgactttcctgttcAlwNIp15A ori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagaaaaccactgacgcgaggaggttcggtcaatggagccaagtttctcaaccatcgagtctcttggaagctttttggcgggacgttccgccaaaaaagcaaaagtctcgttctctaatgcgcgtctggttttgctagagttcttctagtagaataattagtctattttataaagatcAseIBstBIp15A ori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    atttcagtgcaatttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgttactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaataaagtcacgttaaatagagaagtttacatcgtggacttcagtcggggtatgctatattcaacaatgatcacgaacctaagagtggttattttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttAcuISpeIlambda t0 terminator2,1202,1402,1602,1802,2002,2202,2402,260
    gcgagctcgatatcaaattaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggcgctcgagctatagtttaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccAhdIBpmIBsaISacIBanIIEcoRVEco53kIAmpicillin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagAclIAseIBglIFspISfcINmeAIIIAmpicillin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    acgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattcttgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagaPvuIAmpicillin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcgggaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccAclIBcgIDraIScaIXmnIAmpicillin2,8202,8402,8602,8802,9002,9202,9402,960
    ggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgaAcuIApaLIBaeGIBssSIBssSαIAmpicillin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    catactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaaacgtctcattttcgccagatatcgacgtcttaagacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctgtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaactttgcagagtaaaagcggtctatagctgcagaattctgggtgaaagtgtaaattcaacaaaaagattaggcgtatactagttaagttccggcttattcttccgaBclIBsgINdeIZraIAatIIAflIIBciVIBsmBIBspHIEcoRVEsp3IAmpR_promoterTetR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgccgagacgtggaaccactagtttattaagctatcgaacagcattattaccgccgtatgatagtcatcatccacaaagggaaagaagaaatcgctgaactacgagaactagaaggttatgcgttggatttcattttacggggtgtcgcgactcacgtatattacgtaagagatcacAfeIAleIBclIBsmINsiIBstAPITetR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    aaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatttttggaacaaccgtatttttccgattaactaaaagctctcaaagtatgacaaaaagacatccggcacatggatttacatgaaaacgaggtagcgctNruISfcIBsaXIBtgZITetR3,5203,5403,5603,580
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    3598 bp2500275030003250250500750TetR

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    3598