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GGDestX2-Amp
  • Sequence Map
    Editing disabled on read-only sequence.
    gagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactctcccggataaagggtactaaggaagtataaacgtatatgctatgttccgacaatctctctattaaccttaattaaactgacatttgtgtttctataatcatgttttatgcactgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgaTfiIEcoO109IHuman U6 Promoter20406080100120140160
    atcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttGTGGAAAGGACGAAACACCcGAGACCgaGGTCTCagttttagagctaGAAAtagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcTTTTTTgtttagtatacgaatggcattgaactttcataaagctaaagaaccgaaatatatagaaCACCTTTCCTGCTTTGTGGgCTCTGGctCCAGAGtcaaaatctcgatCTTTatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgAAAAAAcaaBsaIBsaINdeIPshAIchimeric guide RNA scaffoldHuman U6 Promoter180200220240260280300320340
    ttagagctagaaatagcaagttaaaataaggctagtccgtTTTTagcgcgtgcgccaattctgcagacaaatggctctagaggtacccgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaatagtaacgccaatagggactaatctcgatctttatcgttcaattttattccgatcaggcaAAAAtcgcgcacgcggttaagacgtctgtttaccgagatctccatgggcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttatcattgcggttatccctgaKpnIPstIXbaIAcc65ICBh360380400420440460480500520
    ttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattGtgcccagtacatgaccttatgggactttcctacttggcagtacatctacgaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaaCacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcBmrINdeISnaBICBh540560580600620640660680700
    tattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaataatcagtagcgataatggtaccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccccgcgcgcggtccgccccgccccgccccgctBsgINcoIStyICBh720740760780800820840860
    ggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgacgctgccttcgccccgtgccccgctccgccgccgcccccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgctgcgacggaagcggggcacggggcgaggcggcggcgCBh8809009209409609801,0001,0201,040
    ctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagctgagcaagaggtaagggtttaagggatggttggttggtggggtattaatgtttaattacctggagcacctgcctgaaatcacttttgagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgactcgttctccattcccaaattccctaccaaccaaccaccccataattacaaattaatggacctcgtggacggactttagtgaaaaBlpIBpmIBfuAIBseRIBspMIPaqCICBh1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tttcaggttGGaccggtgccaccATGgagGGGTCTTCGAGAAGACCTACTAgctaagaattcCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCaaagtccaaCCtggccacggtggTACctcCCCAGAAGCTCTTCTGGATGATcgattcttaagGATCTCGAGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGAgeIBbsIBbsIBclINcoISacIStyIEcoRIEco53kIbGH polyAKozakCBh1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGAgAATAGCAGGCATGCTGGGGAgcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTcTTATCGTCCGTACGACCCCTcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgaEagIEarIMscINotISphIBseYIbGH polyAR-ITR1,4201,4401,4601,4801,5001,5201,5401,560
    cgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgcccgcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggAhdIKasINarIPstISbfISfoISmaISmaISrfISrfIXmaIXmaIPluTIPvuIITspMITspMIR-ITR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacNaeINgoMIVf1 Origin1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaaAloIBsaXIDraIIIf1 Origin1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgactattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgPsiISspI2,1202,1402,1602,1802,2002,2202,2402,260
    gcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctPfoIBsmBIBsmBIEsp3IEsp3IEcoO109I2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttttgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaEarISspIBciVIbla promoterAmpR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaaAcuIXmnIAmpR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtBcgIBtsIBtsIScaIBtsαIBtsαIAmpR2,8202,8402,8602,8802,9002,9202,9402,960
    ctgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcggacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcFspIPvuIBsrDIAmpR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    caaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagccgcggtatcattgcagcactggtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcaccttcggcgccatagtaacgtcgtgacBpmIBsrDISacIINmeAIIIAmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttcccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaaAhdIBmrIAmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    aatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccttaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggpUC ori3,5203,5403,5603,5803,6003,6203,6403,660
    agcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtgtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcacAcuIpUC ori3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgAlwNIBseYIpUC ori3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacBciVIpUC ori4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori4,2204,2404,260
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    4265 bp300032503500375040002505007501000CBhBGHR (Genewiz) (DNA)Human U6 Promoter,U6 - Genewiz (DNA),EcoO109I,TfiI,NdeI,BsaIchimeric guide RNA scaffold,U6 terminator,PshAI,BsaI,PstIXbaI,Acc65I,KpnINdeIBmrISnaBI,NcoI,StyIBsgINmeAIIIBpmI,SacII,BsrDI,BmrIAhdIAcuIpUC ori,AlwNI,BseYIBciVI,AflIII,PciI

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