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EW435 4x(ZNF35/IKZF1tar)-SFFV-GFP
  • Sequence Map
    Editing disabled on read-only sequence.
    ttaagctacaacaaggcaaggcttgaccgacaattgacgattgagtAAACAGGAAATATATAAAGAGctcttcAAACAGGAAATATATAAAGAGgaggtcAAACAGGAAATATATAAAGAGcagaacAAACAGGAAATATATAAAGAGagtctagagggtatataatggatcccaaattcgatgttgttccgttccgaactggctgttaactgctaactcaTTTGTCCTTTATATATTTCTCgagaagTTTGTCCTTTATATATTTCTCctccagTTTGTCCTTTATATATTTCTCgtcttgTTTGTCCTTTATATATTTCTCtcagatctcccatatattacctagggtMfeISapIXbaIBamHIBseRIBspQIZNF35/IKZF1 siteZNF35/IKZF1 siteZNF35/IKZF1 siteZNF35/IKZF1 sitepr...320406080100120140160
    aattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagctgcagtaacgccattttgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaaaccgttcgatcgacgtcattgcggtaaaacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgBmtINheIPstISFFV180200220240260280300320340
    gttgggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccgcagtttcggccccggcccgaggccaagaacagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggcaacccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggcgtcaaagccggggccgggctccggttcttgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttccAloISmaIXmaIAflIIBbvCIEcoRVPpuMITspMIBpu10IBstEIISFFV360380400420440460480500520
    acctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttctgcttcccgagctctataaaagagctcacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGggatccGTCTCAtggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagacgaagggctcgagatattttctcgagtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACcctaggCAGAGTAscINcoISmaIXmaIBamHITspMIBssHIIBssHIIHindIIIMG2SV4S686-1417SFFVGFP540560580600620640660680700
    AAGGGCGAGGAACTTTTTACGGGCGTTGTTCCGATCCTCGTCGAATTGGACGGGGACGTTAATGGGCATAAATTCTCAGTTAGTGGTGAGGGTGAGGGGGACGCGACATATGGGAAACTTACGCTGAAGTTCATATGTACCACCGGAAAATTGCCAGTGCCGTGGCCTACTCTCGTTCCCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGGAGCAGCTTAACCTGCCCCTGCAATTACCCGTATTTAAGAGTCAATCACCACTCCCACTCCCCCTGCGCTGTATACCCTTTGAATGCGACTTCAAGTATACATGGTGGCCTTTTAACGGTCACGGCACCGGATGAGAGCBsmBIBstXIEsp3IK6GE8EL10FT12GV14VP16IL18VE20LD22GD24VN26GH28KF30SV32SG34EG36EG38DA40TY42GK44LT46LK48FI50CT52TG54KL56PV58PW60PT62L686-1417GFP720740760780800820840860
    TCACCACCCTGACATATGGTGTTCAGTGTTTTAGCCGGTATCCAGACCATATGAAACAGCACGATTTTTTTAAATCCGCTATGCCTGAAGGTTACGTACAGGAGCGAACGATATTTTTTAAAGACGACGGAAACTACAAAACTAGAGCCGAGGTAAAATTCGAGGGTGACACTCTAGTGGTGGGACTGTATACCACAAGTCACAAAATCGGCCATAGGTCTGGTATACTTTGTCGTGCTAAAAAAATTTAGGCGATACGGACTTCCAATGCATGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTTTGATGTTTTGATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAAleIBarISnaBIV64TT66LT68YG70VQ72CF74SR76YP78DH80MK82QH84DF86FK88SA90MP92EG94YV96QE98RT100IF102FK104DD106GN108YK110TR112AE114VK116FE118GD120TL122686-1417GFP8809009209409609801,0001,0201,040
    CGTGAACCGGATCGAACTGAAGGGCATAGATTTTAAGGAGGATGGCAATATACTGGGCCACAAGCTCGAATACAACTATAATTCTCATAATGTCTATATCATGGCGGACAAACAGAAAAACGGCATCAAGGTAAACTTTAAAATCCGACACAATATTGAGGACGGCTCTGTCCAGGCACTTGGCCTAGCTTGACTTCCCGTATCTAAAATTCCTCCTACCGTTATATGACCCGGTGTTCGAGCTTATGTTGATATTAAGAGTATTACAGATATAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCATTTGAAATTTTAGGCTGTGTTATAACTCCTGCCGAGACAGGTCBmrIBplISspIVN124RI126EL128KG130ID132FK134ED136GN138IL140GH142KL144EY146NY148NS150HN152VY154IM156AD158KQ160KN162GI164KV166NF168KI170RH172NI174ED176GS178VQ180686-1417GFP1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CTTGCGGACCATTACCAACAGAACACGCCGATAGGGGATGGTCCAGTACTTCTCCCTGATAATCACTATTTGTCTACTCAGTCAGCTCTTAGCAAGGACCCAAACGAAAAACGGGATCACATGGTCCTTCTTGAGTTCGTCACTGCGGCTGGGATAACACTCGGGATGGACGAACGAACGCCTGGTAATGGTTGTCTTGTGCGGCTATCCCCTACCAGGTCATGAAGAGGGACTATTAGTGATAAACAGATGAGTCAGTCGAGAATCGTTCCTGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAACTCAAGCAGTGACGCCGACCCTATTGTGAGCCCTACCTGCTTGBplIPpuMILA182DH184YQ186QN188TP190IG192DG194PV196LL198PD200NH202YL204ST206QS208AL210SK212DP214NE216KR218DH220MV222LL224EF226VT228AA230GI232TL234GM236DE238686-1417GFP1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TGTATAAGaccggTtaggccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccACATATTCtggccAatccggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggAgeIApaIBclIPmeITliIXhoIBseRIPspXIPaeR7IPspOMILY240KT242G*244686-1417BGH polyadenylation sequencec-myc epitopePolyhi...e tagGFP1,4201,4401,4601,4801,5001,5201,5401,560
    ttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacBbsINspISphIBtgZIBGH polyadenylation sequence1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgagClaIBglIIBspDIPvuIIBGH pA1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattcgaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagNaeINgoMIVFRT1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatBarIMscIXbaIZraIAatIIBsaXIBsmBIEsp3IPshAIHygromycinFRT2,1202,1402,1602,1802,2002,2202,2402,260
    aatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgBfuAIBspMIEcoRIBstAPIDraIIIHygromycin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggaggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctNcoIPstIPvuIAsiSIRsrIIHygromycin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattccgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaaggBtgZIBstAPIDraIIIHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    caatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagcAjuISacIIHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    atgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatatactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagccgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgNaeIPfoIPsiINgoMIVSV40 pA3,1603,1803,2003,2203,2403,2603,2803,3003,320
    atcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacatagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgtBsmIBstZ17ISV40 pA3,3403,3603,3803,4003,4203,4403,4603,4803,500
    acatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttctgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaagBsaXIPvuII3,5203,5403,5603,5803,6003,6203,6403,660
    cgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcNspIPciISapIBspQIAflIIIpUC ori3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaapUC ori3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcgcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgpUC ori4,0404,0604,0804,1004,1204,1404,1604,1804,200
    agcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacctcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggAlwNIpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    accgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaBspHIpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    taaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatcatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagAhdIAmp (bla)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaataccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattaBglIBmrIBsaIBsrDIAmp (bla)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    agtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggtcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccFspIPvuIBsrDIAmp (bla)4,9204,9404,9604,9805,0005,0205,0405,060
    ccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacaggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtBcgIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttaXmnIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccaccggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtgSspIBspHIbla promoterAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatgactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaZraIAatIIAlwNIBglIISgrDI5,6205,6405,6605,6805,7005,720
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    5738 bp42504500475050005250550025050075010001250for cloningfor cloning686-14171415-1416EKo309.2 (DNA)EKo130.1 (DNA)EKbR (DNA)pNK018 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)

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    5738