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B582_s23_U6_mus_NLGN3_sgRNA#2_scaffoldv2
  • Sequence Map
Editing disabled on read-only sequence.
AAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGTTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCAclIAcuIXmnIApaLIBssSIBssSαIAmpR20406080100120140160
GGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACGAATTGGCCAGCGCTGCATTACCCTGTTATCCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGCTTAACCGGTCGCGACGTAATGGGACAATAGGAfeIEaeIEarIMscISspIBcoDIBsmAIBspHII-SceII-SceIAmpR180200220240260280300320340
CTAACCGGTGCGTTTTTATGCTTGTAGTATTGTATAATGTTTTTAAGATCCGGAGttctgcAATTCATGtGATCTgcgcctgggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccGATTGGCCACGCAAAAATACGAACATCATAACATATTACAAAAATTCTAGGCCTCaagacgTTAAGTACaCTAGAcgcggaccctcgagtgcccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggAgeIPasISacIBspEIEco53kI2x cHS4seq2Seq2 F360380400420440460480500520
gctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaagctaggctgaaagaGGATCTtagaatgcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgcccctttttcgatccgactttctCCTAGAatcttacPstISrfIRsrII2x cHS4540560580600620640660680700
acacgcgcctgggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggacagcccgggcacggggaaggttgtgcgcggaccctcgagtgcccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccctgtcgggcccgtgccccttccaPasISacISrfIRsrIIEco53kI2x cHS4720740760780800820840860
ggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaagctGGATCataaCTCGAcgtgcaTACTgctggccttttgctcacatgtgagggcctatttcccatgattccttcatatttgcatatacgatacaaggcccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgcccctttttcgaCCTAGtattGAGCTgcacgtATGAcgaccggaaaacgagtgtacactcccggataaagggtactaaggaagtataaacgtatatgctatgttccgNspIPciIPstITfiIBmgBIAflIIIEcoO109I2x cHS4hU6_promoter8809009209409609801,0001,0201,040
tgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatacaatctctctattaaccttaattaaactgacatttgtgtttctataatcatgttttatgcactgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgatagtatacgaatggcattgaactttcataaagctaaagaaccgaaatataNdeIRsaITatIBsaAICviQIhU6_promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
atcttGTGGAAAGGACGAAACACCGGAACAGGCAACATGATTGAgtttcagagctatgctggaaacagcatagcaagttgaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttTTTTgttttagagctagaaatagcaagttaaaataaggctagttagaaCACCTTTCCTGCTTTGTGGCCTTGTCCGTTGTACTAACTcaaagtctcgatacgacctttgtcgtatcgttcaactttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaAAAAcaaaatctcgatctttatcgttcaattttattccgatcaBanIsgRNA scaffold v2guidehU6_promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
ccgtTTTTagcgcgcgctGGTCTAATACCCAATCTCTCGTCTTATCCAGATGTTTTATACGCCTTAATTAATAGGGATAACAGGGTAATATCTTGCCCTGCACGAATACCAGCGACCCCTTGCCCAAATACTTGCCGTGGGCCTCGGCCTGAGAGCCAAAACACTTGATGCGGAAggcaAAAAtcgcgcgcgaCCAGATTATGGGTTAGAGAGCAGAATAGGTCTACAAAATATGCGGAATTAATTATCCCTATTGTCCCATTATAGAACGGGACGTGCTTATGGTCGCTGGGGAACGGGTTTATGAACGGCACCCGGAGCCGGACTCTCGGTTTTGTGAACTACGCCTTBsgIBtgIPacIBceAIBssHIIBssHIII-SceINmeAIIIRK2 trfaseq3I-SceI1,4201,4401,4601,4801,5001,5201,5401,560
GAAGTCGGTGCGCTCCTGCTTGTCGCCGGCATCGTTGCGCCACATCTAGGATCTGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATCTTCAGCCACGCGAGGACGAACAGCGGCCGTAGCAACGCGGTGTAGATCCTAGACGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTADrdIColE1 originRK2 trfa1,5801,6001,6201,6401,6601,6801,7001,7201,740
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTMmeIApaLIBseYIBssSIBssSαIColE1 origin1,7601,7801,8001,8201,8401,8601,8801,9001,920
ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGAMmeIAlwNIColE1 origin1,9401,9601,9802,0002,0202,0402,0602,0802,100
AGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATAcuIBceAIColE1 origin2,1202,1402,1602,1802,2002,2202,2402,260
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGBanIBspHIColE1 originAmpR2,2802,3002,3202,3402,3602,3802,4002,4202,440
TATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTTGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCAhdIBglIBmrIBpmIBsrDIAmpR2,4602,4802,5002,5202,5402,5602,5802,6002,620
CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGAclIFspIBsrDINmeAIIIAmpR2,6402,6602,6802,7002,7202,7402,7602,7802,800
GATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGACTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTBtsIBtsIEaeIPvuIBtsαIBtsαIAmpR2,8202,8402,8602,8802,9002,9202,9402,960
GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAABcgIRsaIScaITatIBsaHICviQIAmpR2,9803,0003,0203,0403,060
  • Plasmid
Editing disabled on read-only sequence.
3074 bp225025002750250500750Seq2 FAmpRSeq2 R (DNA)Seq3 R (DNA)pL1 Seq R (DNA)

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3074