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pCAGGS-Stargazin-mEGFP-iLID
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgatcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacagctagctgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattPvuISpeICAG promoterCAG enhancer20406080100120140160
    cgccaatagggactttccattgacgtcaatgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctgataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacCAG promoterCAG enhancer180200220240260280300320340
    gcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggcggggcgtcatgtagatgcataatcagtagcgataatggtacccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccgcgcgcggtccgccccBsaAISnaBICAG promoterCAG enhancer360380400420440460480500520
    cggggcgggggcgagggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctgccccgcccccgctcccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgcaacggaagcggggcacggggcgaCAG promoter540560580600620640660680700
    ccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggccctttgtgcggcgcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgagcaaagaaaagacaccgacgcactttcggaatttcccgaggccctcccgggaaacacgAfeIApaIPfoIPspOMI720740760780800820840860
    gggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcccccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgcacacgcgctcccctcgcgccggcccccgccacggggcgccacgcccccccgacgAfeIEagI8809009209409609801,0001,0201,040
    gaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgggcctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgccgccagcccgacattggggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcacgccccgcaccgcgccccgagcggcacggcccg1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggCCCCCGGAGCGCCGGCGGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAGGGACTTCCTTTGTCCCccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagccccctccccgcgccgccGGGGGCCTCGCGGCCGCCGACAGCTCCGCGCCGCTCGGCGTCGGTAACGGAAAATACCATTAGCACGCTCTCCCGCGTCCCTGAAGGAAACAGGGMreINaeIPaqCISgrAINgoMIV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGTTTAGACACGCCTCGGCTTTAGACCCTCCGCGGCGGCGTGGGGGAGATCGCCCGCGCCCCGCTTCGCCACGCCGCGGCCGTCCTTCCTTTACCCGCCCCTCCCGGAAGCACGCAGCGGCGCGGCGGCAGGGGAAGAGGGAGAGGTCGGAGCCCCGACAGGCGCCCCCCTGCCGACNaeINgoMIV1,4201,4401,4601,4801,5001,5201,5401,560
    CCTTCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGaattcaccATGGGGCTGTTGGAAGCCCCCCCTGCCCCGTCCCGCCCCAAGCCGAAGACCGCACACTGGCCGCCGAGATCGATCTCGGAGACGATTGGTACAAGTACGGAAGAAGAAAAAGGATGTCGAGGACCCGTTGCACGACCAATAACACGACAGAGTAGTAAAACCGTTTCttaagtggTACCCCGACAAAlwNIMG2LF41740-2750CAG1640St...n1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGATCGAGGTGTTCAAATGCTTTTAACCACCGTTGGTGCTTTCGCTGCCTTCAGCTTGATGACCATCGCTGTGGGAACCGACTATTGGCTGTACTCCAGAGGGGTTTGCAAGACCAAAAGTGTCAGTGAGAATGAAACCAGCAAGAAGAACGAGGAAGTTATGACCCATTCCGGAACTAGCTCCACAAGTTTACGAAAATTGGTGGCAACCACGAAAGCGACGGAAGTCGAACTACTGGTAGCGACACCCTTGGCTGATAACCGACATGAGGTCTCCCCAAACGTTCTGGTTTTCACAGTCACTCTTACTTTGGTCGTTCTTCTTGCTCCTTCAATACTGGGTAAGGCCTAjuIBsaBIBspEIBstXIDR6GV8QM10LL12TT14VG16AF18AA20FS22LM24TI26AV28GT30DY32WL34YS36RG38VC40KT42KS44VS46EN48ET50SK52KN54EE56VM58TH60SG621740-2750Stargazin1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TTATGGAGAACCTGCTGCCTCGAAGGGAACTTCAAAGGTCTGTGCAAGCAAATCGACCACTTTCCGGAAGACGCGGACTACGAAGCTGACACCGCAGAGTATTTCCTCCGGGCCGTGAGGGCCTCGAGTATCTTCCCGATCCTGAGTGTGATCCTGCTTTTCATGGGTGGCCTCTAATACCTCTTGGACGACGGAGCTTCCCTTGAAGTTTCCAGACACGTTCGTTTAGCTGGTGAAAGGCCTTCTGCGCCTGATGCTTCGACTGTGGCGTCTCATAAAGGAGGCCCGGCACTCCCGGAGCTCATAGAAGGGCTAGGACTCACACTAGGACGAAAAGTACCCACCGGAGABbsISfiITliIXhoIBspEIPspXIPaeR7ILW64RT66CC68LE70GN72FK74GL76CK78QI80DH82FP84ED86AD88YE90AD92TA94EY96FL98RA100VR102AS104SI106FP108IL110SV112IL114LF116MG118GL1201740-2750Stargazin1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GCATCGCAGCCAGCGAGTTCTACAAACCGGTGAACTGCAGGAAGAGCTCGAAGAATTCGAAAAGCTTGACACGCCACAACATCATCCTGAGTGCTGGCATCTTCTTCGTGTCTGCAGGTCTTAGTAATATCATCGGGATCATCGTGTATATATCAGCCAATGCTGGAGACCCCTCCGTAGCGTCGGTCGCTCAAGATGTTTGGCCACTTGACGTCCTTCTCGAGCTTCTTAAGCTTTTCGAACTGTGCGGTGTTGTAGTAGGACTCACGACCGTAGAAGAAGCACAGACGTCCAGAATCATTATAGTAGCCCTAGTAGCACATATATAGTCGGTTACGACCTCTGGGGAGAgeIBsaISacISapIBspQIBstBIEco53kICI122AA124SE126FY128KP130VN132CR134KS136SK138NS140KS142LT144RH146NI148IL150SA152GI154FF156VS158AG160LS162NI164IG166II168VY170IS172AN174AG176DP178S1740-2750StargazinStargazin2,1202,1402,1602,1802,2002,2202,2402,260
    CAAGAGTGACTCCAAAAAGAACAGCTACTCCTACGGCTGGTCCTTCTACTTCGGGGCCCTGTCCTTCATCATCGCCGAGATGGTCGGGGTGCTGGCCGTGCACATGTTTATCGACCGCCACAAACAGCTGCGGGCCACGGCCCGCGCCACCGACTACCTCCAGGCCTCCGCCATCGTTCTCACTGAGGTTTTTCTTGTCGATGAGGATGCCGACCAGGAAGATGAAGCCCCGGGACAGGAAGTAGTAGCGGCTCTACCAGCCCCACGACCGGCACGTGTACAAATAGCTGGCGGTGTTTGTCGACGCCCGGTGCCGGGCGCGGTGGCTGATGGAGGTCCGGAGGCGGTAGAloIApaIStuIBsaXIPspOMIK180SD182SK184KN186SY188SY190GW192SF194YF196GA198LS200FI202IA204EM206VG208VL210AV212HM214FI216DR218HK220QL222RA224TA226RA228TD230YL232QA234SA236I1740-2750Stargazin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ACCCGCATCCCCAGCTACCGCTACCGCTACCAGCGCCGCAGCCGCTCCAGCTCGCGCTCCACCGAGCCCTCTCACTCCAGAGACGCCTCGCCCGTGGGCGTGAAGGGCTTCAACACCCTGCCGTCCACGGAGATCTCCATGTACACCCTCAGTAGGGACCCCCTGAAGGCTGCCATGGGCGTAGGGGTCGATGGCGATGGCGATGGTCGCGGCGTCGGCGAGGTCGAGCGCGAGGTGGCTCGGGAGAGTGAGGTCTCTGCGGAGCGGGCACCCGCACTTCCCGAAGTTGTGGGACGGCAGGTGCCTCTAGAGGTACATGTGGGAGTCATCCCTGGGGGACTTCCGACGGTKflIBglIIBseYIBsmBIBsrGIEsp3IPpuMIT238RI240PS242YR244YR246YQ248RR250SR252SS254SR256ST258EP260SH262SR264DA266SP268VG270VK272GF274NT276LP278ST280EI282SM284YT286LS288RD290PL292KA294A1740-2750Stargazin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CCACGCCCACCGCCACCTACAACTCGGACAGGGATAACAGCTTTCTCCAGGTCCACAACTGTATCCAGAAGGACAGCAAGGACTCTcTCCACGCCAACACAGCCAACCGCCGGACCACGCCCGTAgaattcggcATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCGGTGCGGGTGGCGGTGGATGTTGAGCCTGTCCCTATTGTCGAAAGAGGTCCAGGTGTTGACATAGGTCTTCCTGTCGTTCCTGAGAgAGGTGCGGTTGTGTCGGTTGGCGGCCTGGTGCGGGCATcttaagccgTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGBaeIT296TP298TA300TY302NS304DR306DN308SF310LQ312VH314NC316IQ318KD320SK322DS324LH326AN328TA330NR332RT334TP336V1740-2750StargazinmEGFP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGmEGFP2,8202,8402,8602,8802,9002,9202,9402,960
    AGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAPfoImEGFP2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAmEGFP3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTTAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACctcgagGGTGGATCTGgaggttcaggtGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGTTCGAATCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGgagctcCCACCTAGACctccaagtccaTliIXhoIPspXIBpu10IPaeR7IL1E2G3G4S5G6G7S8G93474-3506mEGFPlinkerlinker3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggaagtCTGGCAACCACACTGGAACGGATCGAGAAAAATTTCGTGATTACTGATCCGAGACTGCCTGACAACCCAATCATTTTTGCGAGCGATTCCTTCCTGCAGCTGACAGAATATTCTCGGGAAGAGATCCTGGGGCGCAATTGCCGTTTTCTGCAGGGACCCGAGACAGACCccttcaGACCGTTGGTGTGACCTTGCCTAGCTCTTTTTAAAGCACTAATGACTAGGCTCTGACGGACTGTTGGGTTAGTAAAAACGCTCGCTAAGGAAGGACGTCGACTGTCTTATAAGAGCCCTTCTCTAGGACCCCGCGTTAACGGCAAAAGACGTCCCTGGGCTCTGTCTGGKflIMfeISspIPpuMIG10S11iLid3,5203,5403,5603,5803,6003,6203,6403,660
    GTGCCACTGTTCGGAAAATCAGAGATGCTATTGACAACCAGACTGAAGTGACCGTTCAGCTGATCAATTATACCAAGAGCGGCAAGAAGTTCTGGAACGTGTTCCACCTGCAGCCGATGCGCGATTATAAGGGCGACGTCCAGTACTTCATTGGCGTGCAGCTGGATGGCACCGACACGGTGACAAGCCTTTTAGTCTCTACGATAACTGTTGGTCTGACTTCACTGGCAAGTCGACTAGTTAATATGGTTCTCGCCGTTCTTCAAGACCTTGCACAAGGTGGACGTCGGCTACGCGCTAATATTCCCGCTGCAGGTCATGAAGTAACCGCACGTCGACCTACCGTGGCTBbsIBclIPsiIScaIXcmIPaqCIiLid3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ACGTCTTCATGGCGCCGCTGAGCGTGAGGCGGTCTGCCTGATCAAAAAGACAGCCTTTCAGATTGCTGAGGCAGCGAACGACGAAAATTACTTTtaagcGGCCGCGGTTCTAGATAAGTCGACGGATCTTTTTCCCTCTGCCAAAAATTATGGGGACATCATGAAGCCCCTTGAGTGCAGAAGTACCGCGGCGACTCGCACTCCGCCAGACGGACTAGTTTTTCTGTCGGAAAGTCTAACGACTCCGTCGCTTGCTGCTTTTAATGAAAattcgCCGGCGCCAAGATCTATTCAGCTGCCTAGAAAAAGGGAGACGGTTTTTAATACCCCTGTAGTACTTCGGGGAACTCAccIBclIEagINotISalIXbaIBbvCIBpu10IHincIIiLidMCSCAGGS-R23,8603,8803,9003,9203,9403,9603,9804,0004,020
    CATCTGACTTCTGGCTAATAAAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATATGGGAGGGCAAATCATTTAAAACATCAGAATGAGTATTTGGTTTAGAGTTTGGCAACATATGCCCATATGCTGGCTGCCATGAACAAAGGTAGACTGAAGACCGATTATTTCCTTTAAATAAAAGTAACGTTATCACACAACCTTAAAAAACACAGAGAGTGAGCCTTCCTGTATACCCTCCCGTTTAGTAAATTTTGTAGTCTTACTCATAAACCAAATCTCAAACCGTTGTATACGGGTATACGACCGACGGTACTTGTTTCAjuIPflMI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    GTTGGCTATAAAGAGGTCATCAGTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTGACTTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAACATCCCTAAAATTTTCCTTACATGTTTTACTAGCCAGATTTTTCCTCCTCAACCGATATTTCTCCAGTAGTCATATACTTTGTCGGGGGACGACAGGTAAGGAATAAGGTATCTTTTCGGAACTGAACTCCAATCTAAAAAAAATATAAAACAAAACACAATAAAAAAAGAAATTGTAGGGATTTTAAAAGGAATGTACAAAATGATCGGTCTAAAAAGGAGGA4,2204,2404,2604,2804,3004,3204,3404,360
    CTCCTGACTACTCCCAGTCATAGCTGTCCCTCTTCTCTTATGGagatccctcgacctgcagcccaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaGAGGACTGATGAGGGTCAGTATCGACAGGGAGAAGAGAATACCtctagggagctggacgtcgggttcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggatlac promoterLacOM13-rev4,3804,4004,4204,4404,4604,4804,5004,5204,540
    atgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcggatccgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggcttactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgcctaggcgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgaBamHIBsaBISV40 Ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgattgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtBplIBplIPsiISfiIStuIAvrIISV40 Ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccgctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctaggcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgcBsmISapIBamHIBsaXIBspQISV40 Ori4,9204,9404,9604,9805,0005,0205,0405,060
    agcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgColE1 origin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagttacgagtgcgacatccatagagtcaaDrdIColE1 origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgcgccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgAlwNIBseYIColE1 origin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgColE1 origin5,6205,6405,6605,6805,7005,7205,7405,760
    agaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaatctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttColE1 origin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggAhdIBsaIAmp_R5,9605,9806,0006,0206,0406,0606,0806,1006,120
    agatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgAclIFspIAmp_R6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttaagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatPvuIAmp_R6,3206,3406,3606,3806,4006,4206,4406,460
    ctgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcggacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcAclIScaIAmp_R6,4806,5006,5206,5406,5606,5806,6006,6206,640
    aaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatAmp_R6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ctcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctggagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggacSspISexAI6,8406,8606,8806,9006,9206,940
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    6955 bp500055006000650050010001500MCSCAG enhancermEGFP R1 (DNA)

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    6955