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EW378(ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP
  • Sequence Map
    Editing disabled on read-only sequence.
    agatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggtt20406080100120140160
    tgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggAhdIBsaIBsrDIAmp180200220240260280300320340
    agccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttctcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagAclIFspIBsrDIAmp360380400420440460480500520
    attcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagataagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctAmp540560580600620640660680700
    tgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatgAclIBcgIXmnIAmp720740760780800820840860
    cgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattagcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataatAmp8809009209409609801,0001,0201,040
    ttgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaatCspCI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    accaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctatggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggatAloIPsiI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    atcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaacctagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattgg1,4201,4401,4601,4801,5001,5201,5401,560
    accacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcactggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgFspIPvuII1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatattgtctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataacaBssHIIBII(R)5'TR31...r5'...)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttgtBsiWI31bp spacer5'TR(1)5'TR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatcccagagccggcagcgtgcggggattttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtagggtctcggccgtcgcacgcccctPasIcore insul2,1202,1402,1602,1802,2002,2202,2402,260
    cagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgacgattgagtaacactgtatctagtctacctacagggctcAAAATTGCAAGGATATATgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaactgctaactcattgtgacatagatcagatggatgtcccgagTTTTAACGTTCCTATATAMfeISfiISrfIcore insulZNF35/... site2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AAAGAGctcttcAAAATTGCAAGGATATATAAAGAGgaggtcAAAATTGCAAGGATATATAAAGAGcagaacAAAATTGCAAGGATATATAAAGAGgccttccgtatcagtctagagggtatataatggatcccatcgcgtctcagcctcactttgagctcctccacacgaattcTTTCTCgagaagTTTTAACGTTCCTATATATTTCTCctccagTTTTAACGTTCCTATATATTTCTCgtcttgTTTTAACGTTCCTATATATTTCTCcggaaggcatagtcagatctcccatatattacctagggtagcgcagagtcggagtgaaactcgaggaggtgtgcttaagSapIXbaIBspQIZNF35/250 siteZNF35/250 siteZNF35/250 site2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cccgataccgtcgattcaaggagcttgcttgttctttttgcagaagctcagaataaacgctcaactttggcagatctaccaagcttggtaccgagctcggatccaactcgaggccaccatgccagagccagcgaagtctgctcccgccccgaaaaagggctccaagaaggcggtggggctatggcagctaagttcctcgaacgaacaagaaaaacgtcttcgagtcttatttgcgagttgaaaccgtctagatggttcgaaccatggctcgagcctaggttgagctccggtggtacggtctcggtcgcttcagacgagggcggggctttttcccgaggttcttccgccacKpnITliIXhoIBglIIPspXIAcc65IPaeR7IHindIIIMP2EP4AK6SA8PA10PK12KG14SK16KA18V2744-3853H2BXenopus globin 5'-UTR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    actaaggcgcagaagaaaggcggcaagaagcgcaagcgcagccgcaaggagagctattccatctatgtgtacaaggttctgaagcaggtccaccctgacaccggcatttcgtccaaggccatgggcatcatgaattcgtttgtgaacgacattttcgagcgcatcgcaggtgaggtgattccgcgtcttctttccgccgttcttcgcgttcgcgtcggcgttcctctcgataaggtagatacacatgttccaagacttcgtccaggtgggactgtggccgtaaagcaggttccggtacccgtagtacttaagcaaacacttgctgtaaaagctcgcgtagcgtccactccBsrGIPaqCIT20KA22QK24KG26GK28KR30KR32SR34KE36SY38SI40YV42YK44VL46KQ48VH50PD52TG54IS56SK58AM60GI62MN64SF66VN68DI70FE72RI74AG76E2744-3853H2B2,8202,8402,8602,8802,9002,9202,9402,960
    cttcccgcctggcgcattacaacaagcgctcgaccatcacctccagggagatccagacggccgtgcgcctgctgctgcctggggagttggccaagcacgccgtgtccgagggtactaaggccatcaccaagtacaccagcgctaaggatccccgggtaccggtcGTCTCAAAGGGgaagggcggaccgcgtaatgttgttcgcgagctggtagtggaggtccctctaggtctgccggcacgcggacgacgacggacccctcaaccggttcgtgcggcacaggctcccatgattccggtagtggttcatgtggtcgcgattcctaggggcccatggccagCAGAGTTTCCCAfeIAfeIAgeIKpnIMscIAcc65IBpu10IVS2KG43140-3853A78SR80LA82HY84NK86RS88TI90TS92RE94IQ96TA98VR100LL102LP104GE106LA108KH110AV112SE114GT116KA118IT120KY122TS124AK126DP128RV130PV132VS134KG1362744-3853H2BlinkerGFP2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CGAGGAACTTTTTACGGGCGTTGTTCCGATCCTCGTCGAATTGGACGGGGACGTTAATGGGCATAAATTCTCAGTTAGTGGTGAGGGTGAGGGGGACGCGACATATGGGAAACTTACGCTGAAGTTCATATGTACCACCGGAAAATTGCCAGTGCCGTGGCCTACTCTCGTCACCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGGAGCAGCTTAACCTGCCCCTGCAATTACCCGTATTTAAGAGTCAATCACCACTCCCACTCCCCCTGCGCTGTATACCCTTTGAATGCGACTTCAAGTATACATGGTGGCCTTTTAACGGTCACGGCACCGGATGAGAGCAGTGGEE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK26FS28VS30GE32GE34GD36AT38YG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT623140-3853EE138LF140TG142VV144PI146LV148EL150DG152DV154NG156HK158FS160VS162GE164GE166GD168AT170YG172KL174TL176KF178IC180TT182GK184LP186VP188WP190TL192VT1942744-3853GFP3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ACCCTGACATATGGTGTTCAGTGTTTTAGCCGGTATCCAGACCATATGAAACAGCACGATTTTTTTAAATCCGCTATGCCTGAAGGTTACGTACAGGAGCGAACGATATTTTTTAAAGACGACGGAAACTACAAAACTAGAGCCGAGGTAAAATTCGAGGGTGACACTCTCGTGATGGGACTGTATACCACAAGTCACAAAATCGGCCATAGGTCTGGTATACTTTGTCGTGCTAAAAAAATTTAGGCGATACGGACTTCCAATGCATGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTTTGATGTTTTGATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAGCACTAleIBarISnaBITL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF84KS86AM88PE90GY92VQ94ER96TI98FF100KD102DG104NY106KT108RA110EV112KF114EG116DT118LV1203140-3853TL196TY198GV200QC202FS204RY206PD208HM210KQ212HD214FF216KS218AM220PE222GY224VQ226ER228TI230FF232KD234DG236NY238KT240RA242EV244KF246EG248DT250LV2522744-3853GFP3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ACCGGATCGAACTGAAGGGCATAGATTTTAAGGAGGATGGCAATATACTGGGCCACAAGCTCGAATACAACTATAATTCTCATAATGTCTATATCATGGCGGACAAACAGAAAAACGGCATCAAGGTAAACTTTAAAATCCGACACAATATTGAGGACGGCTCTGTCCAGCTTGCTGGCCTAGCTTGACTTCCCGTATCTAAAATTCCTCCTACCGTTATATGACCCGGTGTTCGAGCTTATGTTGATATTAAGAGTATTACAGATATAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCATTTGAAATTTTAGGCTGTGTTATAACTCCTGCCGAGACAGGTCGAACGNR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142YN144YN146SH148NV150YI152MA154DK156QK158NG160IK162VN164FK166IR168HN170IE172DG174SV176QL178A3140-3853NR254IE256LK258GI260DF262KE264DG266NI268LG270HK272LE274YN276YN278SH280NV282YI284MA286DK288QK290NG292IK294VN296FK298IR300HN302IE304DG306SV308QL310A2744-3853GFP3,5203,5403,5603,5803,6003,6203,6403,660
    GGACCATTACCAACAGAACACGCCGATAGGGGATGGTCCAGTACTTCTCCCTGATAATCACTATTTGTCTACTCAGTCAGCTCTTAGCAAGGACCCAAACGAAAAACGGGATCACATGGTCCTTCTTGAGTTCGTCACTGCGGCTGGGATAACACTCGGGATGGACGAACTGTATCCTGGTAATGGTTGTCTTGTGCGGCTATCCCCTACCAGGTCATGAAGAGGGACTATTAGTGATAAACAGATGAGTCAGTCGAGAATCGTTCCTGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAACTCAAGCAGTGACGCCGACCCTATTGTGAGCCCTACCTGCTTGACATAPpuMIEcoO109ID180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY200LS202TQ204SA206LS208KD210PN212EK214RD216HM218VL220LE222FV224TA226AG228IT230LG232MD234EL236Y3140-3853D312HY314QQ316NT318PI320GD322GP324VL326LP328DN330HY332LS334TQ336SA338LS340KD342PN344EK346RD348HM350VL352LE354FV356TA358AG360IT362LG364MD366EL368Y2744-3853GFP3,6803,7003,7203,7403,7603,7803,8003,8203,840
    AAGtagctagcgaattcgaatttaaatcggcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctatTTCatcgatcgcttaagcttaaatttagccggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataBmtINheISwaIBstBI*1K238K370BGH polyadenylation sequenceMCS3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagBbsINotIXmnIBGH polyadenylation sequencepSV405, 299 F4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttattaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaatSexAIpSV404,2204,2404,2604,2804,3004,3204,3404,360
    tttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagttaaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcactactttttcggacttgagtggcgctgcagacagctcttcaaBclIPmlIZraIAatIIAvrIIPshAIpSV40Hygro4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagPflFITth111IHygro2, 563 F4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgaggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctAsiSIHygro4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacgggPflFITth111IHygro4,9204,9404,9604,9805,0005,0205,0405,060
    cgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgcgcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgAjuIHygro5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgSacIIHygro3, 135 F5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataggtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgccatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatccagctgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgSalIHygroWPRE6, 730 R5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagBsmIHpaIPsiISV40 pASV40 pA(1)WPRE5,6205,6405,6605,6805,7005,7205,7405,760
    ttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttcccaaactgggttcttatatcccttgctctggtcaaccaggttgcagggtttcctgtcctcacagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaagggtttgacccaagaatatagggaacgagaccagttggtccaacgtcccaaaggacaggagtgtcBsaISexAISV40 pASV4...1)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggctcaaagagcagcgagaagcgttcagaggaaagcgatcccgtgccaccttccccgtgcccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtccgagtttctcgtcgctcttcgcaagtctcctttcgctagggcacggtggaaggggcacggApaIEcoNIPspOMIEcoO109Icore insul(1) Geneious name: core insul(1)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggggctttgggggggggctgtccctgatatctataacaagaaaatatatatataataaggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccccgaaacccccccccgacagggactatagatattgttcttttatatatatattattcPasISrfIEcoRVcore insul(1) Geneious name: core insul(1)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgaaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgact6,3206,3406,3606,3806,4006,4206,4406,460
    gatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacBsmI3'TR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccaccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtMscI6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctgggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacBsgI6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcactacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtBssHIIpLac(F)7,0207,0407,0607,0807,1007,1207,1407,160
    cattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagcgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgSapIBspQIPvuII7,1807,2007,2207,2407,2607,2807,3007,3207,340
    tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccPciIAflIII7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagc7,5407,5607,5807,6007,6207,6407,6607,6807,700
    ttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaaAlwNI7,7207,7407,7607,7807,8007,8207,8407,860
    gaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcct7,8807,9007,9207,9407,9607,9808,0008,0208,040
    tctcaagaagagttct8,058
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8058 bp60006500700075005001000150020005'TR5'TR(1)AmppLac(F)EKo147.1 (DNA)SV40PA_R (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),AhdI,BsaI,BsrDIFspI,AclI,BsrDIBcgIAclIXmnICspCIPsiIAloIFrtF (DNA)FlpInR (DNA)FspI,PvuIIM13-40FOR Genewiz (DNA),M13F Genewiz (DNA),BssHIIBII(R),5'TRBsiWI31bp spacer,5'TRSrfIcore insul(1) Geneious name: core insul(1)PasIEcoRVBsmI3'TR,ES003-3 C->AMscIBsgIM13R Genewiz (DNA),BssHIIPvuIIpUCori5F (DNA),BspQI,SapI,AflIII,PciIpUCoriR (DNA)AlwNI

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    8058