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AAV-KrasG12V/sgKras/Cre
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    ACGCGTgatatcagatctgttacgtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgaTGCGCActatagtctagacaatgcatctattcatcgtaccgcccaattagtaattgatgttccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctMluIMscIBglIIITR20406080100120140160
    gcgagcgcgcagctgcctgcaggtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgacgctcgcgcgtcgacggacgtccagctgagatctcctaggggcccatggctcgagcttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctKpnISalIXbaIBamHIAcc65I180200220240260280300320340
    tcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgFspIRep_Ori360380400420440460480500520
    ttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcaacggtcgcggaatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagNaeINgoMIVRep_Ori540560580600620640660680700
    gccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaaccgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgagatagagcccgataagaaaactaaatattccctaaaacggctaaagccagataaccaattttttactcgactaaattgtttttaaattgAloIRep_Ori720740760780800820840860880
    gcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgaRep_Ori9009209409609801,0001,0201,0401,060
    gcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttZraIAatII1,0801,1001,1201,1401,1601,1801,2001,2201,240
    tatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacAmpR1,2601,2801,3001,3201,3401,3601,3801,4001,420
    ggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtctXmnIAmpR1,4401,4601,4801,5001,5201,5401,5601,5801,600
    atgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacaAmpR1,6201,6401,6601,6801,7001,7201,7401,7601,780
    aactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctFspINmeAIIIAmpR1,8001,8201,8401,8601,8801,9001,9201,940
    ccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcBsaIAmpR1,9601,9802,0002,0202,0402,0602,0802,1002,120
    ctgagataggtgcctcactgattaagtattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagagactctatccacggagtgactaattcataaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctAmpR2,1402,1602,1802,2002,2202,2402,2602,2802,300
    ccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaaRep-origin2,3202,3402,3602,3802,4002,4202,4402,4602,480
    ctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttgatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaaRep-origin2,5002,5202,5402,5602,5802,6002,6202,6402,660
    cgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctgcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaRep-origin2,6802,7002,7202,7402,7602,7802,8002,8202,840
    ttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataacaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattgRep-origin2,8602,8802,9002,9202,9402,9602,9803,0003,020
    cgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcacSapIBspQI3,0403,0603,0803,1003,1203,1403,1603,1803,200
    agcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggcagctgcgcgctcgaactttcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgaacgtacggacgtccgtcgacgcgcgagcttgaaHindIII3,2203,2403,2603,2803,3003,3203,3403,3603,380
    catgcctgccgaccttccccaggtcacgatccggacggcgggtgagttcacattttarcagccggacgtgcaractccgctggtggtctaacgtcggttaggtcccttgaatcacgggacatatgttggtgttggaggtcctgcaggcagctgcgcgctcgctcgctcactgaggccggtacggacggctggaaggggtccagtgctaggcctgccgcccactcaagtgtaaaatygtcggcctgcacgtytgaggcgaccaccagattgcagccaatccagggaacttagtgccctgtatacaaccacaacctccaggacgtccgtcgacgcgcgagcgagcgagtgactccggcNdeIBspEIEcoNIITR3,4003,4203,4403,4603,4803,5003,5203,5403,560
    cccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctggaggggtggagtcgtgacgtaaagatctgatatcatcgatcgcgatgcattaattaagcggccgccatggCAGTTCTTTTGTATCCgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacctccccacctcagcactgcatttctagactatagtagctagcgctacgtaattaattcgccggcggtaccGTCAAGAAAACATAGGBtgIClaIEagIMscINcoINotINruINsiIPacIBglIIBspDIITRKrasH....1kb3,5803,6003,6203,6403,6603,6803,7003,720
    ATTTGTCTCTTTATCTGCAATAATATATGTTCTACAAGGGAAAGGAGATAAAGTGTATATATTAATTTTATTTCAAAAATGTTTGGAAAAGCAGACTAGGAAACAACAGTGTTGTATTCAGGGCTGTTAACTGTATACAGTGCCAGCACTTAGTGCACCTGTTGGGAGTGCAGTGTATTAAACAGAGAAATAGACGTTATTATATACAAGATGTTCCCTTTCCTCTATTTCACATATATAATTAAAATAAAGTTTTTACAAACCTTTTCGTCTGATCCTTTGTTGTCACAACATAAGTCCCGACAATTGACATATGTCACGGTCGTGAATCACGTGGACAACCCTCACGTCACATAHpaIBstAPIBstZ17IKrasHR 2.1kb3,7403,7603,7803,8003,8203,8403,8603,8803,900
    TATAAGCAAGGCAGAAGTCACAGAGGTTACTGAGAAACTCTTTTATGTTCTTTACATTTTTTTTCTTGCCAAAAATAGTCTTTATCTCTGAATTTCATTCAATTTTATTTCCTTGTACAGAGGTACTGTTTTTAGTACTCAAAGGATTATATGTTAAAATTGATCTGATCCTATACCAATATTCGTTCCGTCTTCAGTGTCTCCAATGACTCTTTGAGAAAATACAAGAAATGTAAAAAAAAGAACGGTTTTTATCAGAAATAGAGACTTAAAGTAAGTTAAAATAAAGGAACATGTCTCCATGACAAAAATCATGAGTTTCCTAATATACAATTTTAACTAGACTAGGATATGGTKrasHR 2.1kb3,9203,9403,9603,9804,0004,0204,0404,0604,080
    AATATTTAAACCCATTTCCCACAGATAGTTTATAGATTAAAATGCCATCAAATATTATCTTTATTTGCTTCATTGCCTAAATATGTCTTTCCCCAGCACAGTGCAGTTTTGACACCAGCTTCGGCTTCCTATTTTGTTGCCCTTTATTGCAGAACTGCTCTGATGGCTTTAAAACAGTTTATAAATTTGGGTAAAGGGTGTCTATCAAATATCTAATTTTACGGTAGTTTATAATAGAAATAAACGAAGTAACGGATTTATACAGAAAGGGGTCGTGTCACGTCAAAACTGTGGTCGAAGCCGAAGGATAAAACAACGGGAAATAACGTCTTGACGAGACTACCGAAATTTTGTCAKrasHR 2.1kb4,1004,1204,1404,1604,1804,2004,2204,2404,260
    TCTCTGCAGTTGTTGGCTCCAACACAGATGTTCTTAGGCTACCTAACTTCTAACTTTTAATATCCAGTCAACAAAGAATACCGCAAGGGTAGGTGTTGGGATAGCTGTCGACAAGCTCATGCGGGTGTGTCCACAGGGTATAGCGTACTATGCAGAATATTTGTACTGAGTGAAGTCAAGAGACGTCAACAACCGAGGTTGTGTCTACAAGAATCCGATGGATTGAAGATTGAAAATTATAGGTCAGTTGTTTCTTATGGCGTTCCCATCCACAACCCTATCGACAGCTGTTCGAGTACGCCCACACAGGTGTCCCATATCGCATGATACGTCTTATAAACATGACTCACTTCAGTBarISalIKrasHR 2.1kb4,2804,3004,3204,3404,3604,3804,4004,4204,440
    TGATACATTCCTTTGAGAGCCATTAGCTGCTACAAAACAGTAATCTGGCTGTTTAGATCAACAAGCTAAATGATAGAAGATGAAAGTACTGGTTTCCATGTATTTTTATTAAGTGTTGATGAGAAAGTTGTAAGTGACTTACAGGTTACTCTGTACATCTGTAGTCACTGAATTCGGAACTATGTAAGGAAACTCTCGGTAATCGACGATGTTTTGTCATTAGACCGACAAATCTAGTTGTTCGATTTACTATCTTCTACTTTCATGACCAAAGGTACATAAAAATAATTCACAACTACTCTTTCAACATTCACTGAATGTCCAATGAGACATGTAGACATCAGTGACTTAAGCCTKrasHR 2.1kb4,4604,4804,5004,5204,5404,5604,5804,6004,620
    ATATCTTAGAGTTTTACACACctAGGTGAGTGTTAAAATATTGATAAAGTTTTTGATAATCTTGTGTGAGACATGTTCTAATTTAGTTGTATTTTATTATTTTTATTGTAAGGCCTGCTGAAAATGACTGAGTATAAACTaGTaGTcGTTGGAGCTGTTGGCGTCGGGAAATCGGCCCTATAGAATCTCAAAATGTGTGgaTCCACTCACAATTTTATAACTATTTCAAAAACTATTAGAACACACTCTGTACAAGATTAAATCAACATAAAATAATAAAAATAACATTCCGGACGACTTTTACTGACTCATATTTGAtCAtCAgCAACCTCGACAACCGCAGCCCTTTAGCCGGGAleISpeIStuIAvrIIM1T2E3Y4K5L6V7V8V9G10A11V12G13V14G15K16S17A184752-4863KrasHR 2.1kbexon2sgKrasBarcode4,6404,6604,6804,7004,7204,7404,7604,7804,800
    TTACTATTCAGCTAATTCAGAATCACTTTGTGGATGAGTATGACCCTACGATAGAGGTAACGCTGCTCTACAGTCTGCGTaCGCTTGTAAAGGACGGCAGCCAGCCGCTTTGAAAAAGATATCATTTTTATATTTATTAGAAAATTATATTGAAAGTTATTTCAGTTATATGTGATGTAATGATAAGTCGATTAAGTCTTAGTGAAACACCTACTCATACTGGGATGCTATCTCCATTGCGACGAGATGTCAGACGCAtGCGAACATTTCCTGCCGTCGGTCGGCGAAACTTTTTCTATAGTAAAAATATAAATAATCTTTTAATATAACTTTCAATAAAGTCAATATACACTACABsiWIL19T20I21Q22L23I24Q25N26H27F28V29D30E31Y32D33P34T35I36E374752-4863KrasHR 2.1kbexon24,8204,8404,8604,8804,9004,9204,9404,9604,980
    CCTTTAGTTCCAAGGCTTTAAACTGGGTGTTAGGGAACCATAGGTGCAAGAAAGTCCACTTCTCATGAGAGCTCACCACAGAGAAAGAAAGTCCACTTCTCATGAGAGCTTACCACAGAGAGTAAGGTCCAAGATGCCATCCGTGCTTTGTATTTGCATTTTGATAAATAAATTTCATGGAAATCAAGGTTCCGAAATTTGACCCACAATCCCTTGGTATCCACGTTCTTTCAGGTGAAGAGTACTCTCGAGTGGTGTCTCTTTCTTTCAGGTGAAGAGTACTCTCGAATGGTGTCTCTCATTCCAGGTTCTACGGTAGGCACGAAACATAAACGTAAAACTATTTATTTAAAGTAKrasHR 2.1kb5,0005,0205,0405,0605,0805,1005,1205,1405,160
    GATTTTGTGAGAGTTAAGAATATAGCTTTGCCTTTGATCTATTCCTGAAAGGTTTGTTGTTGTTTTATGGGTTTGTTGTTATTGTGTTGTTGTTTGGTGTCTTTTAGGCAGGGTCTTACTATGCAGCCCTGACCGGCCTAGAACTCAACTATGTAGACCAGACTAGCTCTGAATTCTCCTAAAACACTCTCAATTCTTATATCGAAACGGAAACTAGATAAGGACTTTCCAAACAACAACAAAATACCCAAACAACAATAACACAACAACAAACCACAGAAAATCCGTCCCAGAATGATACGTCGGGACTGGCCGGATCTTGAGTTGATACATCTGGTCTGATCGAGACTTAAGAGKrasHR 2.1kb5,1805,2005,2205,2405,2605,2805,3005,3205,340
    AGAGATCCATCTACCTGCCTCTGCTGCCCAGATGCTGCGATTAAAGATGTACACCACCATGTACAGTGAAAGTTACTCTTTTGAAAACACTTTATTGTTATAGATTACTTGGAATGTTGATGTGATGAGAAAATTTGATTATACTAGGAAAATAGAATCACTTGATGGTTGAGCTTTTTCTCTAGGTAGATGGACGGAGACGACGGGTCTACGACGCTAATTTCTACATGTGGTGGTACATGTCACTTTCAATGAGAAAACTTTTGTGAAATAACAATATCTAATGAACCTTACAACTACACTACTCTTTTAAACTAATATGATCCTTTTATCTTAGTGAACTACCAACTCGAAAAKrasHR 2.1kb5,3605,3805,4005,4205,4405,4605,4805,500
    TTTTTAAGACTTATTTATTTTATGTAAGTGAGTACACTGCTTCTCTCTTCAGACACACCAGAAGAGGGCATCAGATCCCATTAAGATGGCTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAAGGCAGTCAGTGCTCTTTTTTTGTTTAAAGATTTATTTATAAAAATTCTGAATAAATAAAATACATTCACTCATGTGACGAAGAGAGAAGTCTGTGTGGTCTTCTCCCGTAGTCTAGGGTAATTCTACCGACACTCGGTGGTACACCAACGACCCTTAACTTGAGTCCTGGAGACCTTTCCGTCAGTCACGAGAAAAAAACAAATTTCTAAATAAATAXcmIBstXIPflMIBstAPIKrasHR 2.1kb5,5205,5405,5605,5805,6005,6205,6405,6605,680
    TTTTATGAATACAGTGTCACTGTCTTCATACACACAAGAGctcgaGCTGTGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAAAAATACTTATGTCACAGTGACAGAAGTATGTGTGTTCTCgagctCGACACTCCCGGATAAAGGGTACTAAGGAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAACCTTAATTAAACTGACATTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATBbsITliIXhoIPspXIPaeR7IU6 promoterKrasHR 2.1kb5,7005,7205,7405,7605,7805,8005,8205,8405,860
    ATTTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACCAAACACCGACTGAGTATAAACTTGTGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTATAAAGAACCCATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAACTTTCATAAAGCTAAAGAACCGAAATATATAGAACACCTTTCCTGGTTTGTGGCTGACTCATATTTGAACACCCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATNdeIU6 promoterChimeric RNA (& trRNA)gmKras5,8805,9005,9205,9405,9605,9806,0006,0206,040
    GTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTGTTTGGCGCGCCTATGGCTTAATTAACATGGCGGCCGCATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTCAGGCAATAGTTGAACTTTTTCACCGTGGCTCAGCCACGAAAAAACAAACCGCGCGGATACCGAATTAATTGTACCGCCGGCGTAAGATGGCCCATCCCCTCCGCGAAAAGGGTTCCGTCAGACCTCGTACGCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGAGAAscIBsmIEagINotIPacIPGK promoterChimeric RNA (& trRNA)6,0606,0806,1006,1206,1406,1606,1806,2006,220
    GGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACCGGAGCGTGTGTAAGGTGTAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCGGTGGAAGATGAGGAGGGGATCAGTCCTTCAAGGGGGGGCGGGGCGTCGAGCGCAGCACGTCCTGCACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTAgeISpeIBseRIPGK promoter6,2406,2606,2806,3006,3206,3406,3606,3806,400
    CAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGGTCGTGGCGACTCGTTACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGCGAAAGACCCGAGTCTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCCCGCCCCGCCCGCGGGCTTCCAGGAGGCCTCCGGGCCGTAAGACGTGCBlpIBsmIStuIBspEIPGK promoter6,4206,4406,4606,4806,5006,5206,5406,5606,580
    CTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGACCCGGGATGCCCAAGAAGAAGAGGAAGGTGTCCAATTTACTGACCGTACACCAAAATTTGCCTGCATTACCGGTCGATGCAACGAGTGATGAGGTTCGCAAGAACCTGATGGACATGTTCAGGGATCGAAGTTTTCGCGTGCAGACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCTGGGCCCTACGGGTTCTTCTTCTCCTTCCACAGGTTAAATGACTGGCATGTGGTTTTAAACGGACGTAATGGCCAGCTACGTTGCTCACTACTCCAAGCGTTCTTGGACTACCTGTACAAGTCCCTAGAgeIKflIBseRIMP2KK4KR6KV8SN10LL12TV14HQ16NL18PA20LP22VD24AT26SD28EV30RK32NL34MD36MF38RD406644-7695CrePGK promoter6,6006,6206,6406,6606,6806,7006,7206,7406,760
    GCCAGGCGTTTTCTGAGCATACCTGGAAAATGCTTCTGTCCGTTTGCCGGTCGTGGGCGGCATGGTGCAAGTTGAATAACCGGAAATGGTTTCCCGCAGAACCTGAAGATGTTCGCGATTATCTTCTATATCTTCAGGCGCGCGGTCTGGCAGTAAAAACTATCCAGCAACATTTGGGCGGTCCGCAAAAGACTCGTATGGACCTTTTACGAAGACAGGCAAACGGCCAGCACCCGCCGTACCACGTTCAACTTATTGGCCTTTACCAAAGGGCGTCTTGGACTTCTACAAGCGCTAATAGAAGATATAGAAGTCCGCGCGCCAGACCGTCATTTTTGATAGGTCGTTGTAAACCCNruIXmnIRQ42AF44SE46HT48WK50ML52LS54VC56RS58WA60AW62CK64LN66NR68KW70FP72AE74PE76DV78RD80YL82LY84LQ86AR88GL90AV92KT94IQ96QH98LG1006644-7695Cre6,7806,8006,8206,8406,8606,8806,9006,9206,940
    CCAGCTAAACATGCTTCATCGTCGGTCCGGGCTGCCACGACCAAGTGACAGCAATGCTGTTTCACTGGTTATGCGGCGGATCCGAAAAGAAAACGTTGATGCCGGTGAACGTGCAAAACAGGCTCTAGCGTTCGAACGCACTGATTTCGACCAGGTTCGTTCACTCATGGAAAATAGCGGTCGATTTGTACGAAGTAGCAGCCAGGCCCGACGGTGCTGGTTCACTGTCGTTACGACAAAGTGACCAATACGCCGCCTAGGCTTTTCTTTTGCAACTACGGCCACTTGCACGTTTTGTCCGAGATCGCAAGCTTGCGTGACTAAAGCTGGTCCAAGCAAGTGAGTACCTTTTATCGBamHIBstBIRsrIISexAIQL102NM104LH106RR108SG110LP112RP114SD116SN118AV120SL122VM124RR126IR128KE130NV132DA134GE136RA138KQ140AL142AF144ER146TD148FD150QV152RS154LM156EN158S6644-7695Cre6,9606,9807,0007,0207,0407,0607,0807,1007,120
    GATCGCTGCCAGGATATACGTAATCTGGCATTTCTGGGGATTGCTTATAACACCCTGTTACGTATAGCCGAAATTGCCAGGATCAGGGTTAAAGATATCTCACGTACTGACGGTGGGAGAATGTTAATCCATATTGGCAGAACGAAAACGCTGGTTAGCACCGCAGGTGTAGAGAAGGCTAGCGACGGTCCTATATGCATTAGACCGTAAAGACCCCTAACGAATATTGTGGGACAATGCATATCGGCTTTAACGGTCCTAGTCCCAATTTCTATAGAGTGCATGACTGCCACCCTCTTACAATTAGGTATAACCGTCTTGCTTTTGCGACCAATCGTGGCGTCCACATCTCTTCCAsiSIPaqCID160RC162QD164IR166NL168AF170LG172IA174YN176TL178LR180IA182EI184AR186IR188VK190DI192SR194TD196GG198RM200LI202HI204GR206TK208TL210VS212TA214GV216EK2186644-7695Cre7,1407,1607,1807,2007,2207,2407,2607,280
    CACTTAGCCTGGGGGTAACTAAACTGGTCGAGCGATGGATTTCCGTCTCTGGTGTAGCTGATGATCCGAATAACTACCTGTTTTGCCGGGTCAGAAAAAATGGTGTTGCCGCGCCATCTGCCACCAGCCAGCTATCAACTCGCGCCCTGGAAGGGATTTTTGAAGCAACTCATCGATTGTGAATCGGACCCCCATTGATTTGACCAGCTCGCTACCTAAAGGCAGAGACCACATCGACTACTAGGCTTATTGATGGACAAAACGGCCCAGTCTTTTTTACCACAACGGCGCGGTAGACGGTGGTCGGTCGATAGTTGAGCGCGGGACCTTCCCTAAAAACTTCGTTGAGTAGCTAAClaIBspDIAL220SL222GV224TK226LV228ER230WI232SV234SG236VA238DD240PN242NY244LF246CR248VR250KN252GV254AA256PS258AT260SQ262LS264TR266AL268EG270IF272EA274TH276RL2786644-7695Cre7,3007,3207,3407,3607,3807,4007,4207,4407,460
    GATTTACGGCGCTAAGGATGACTCTGGTCAGAGATACCTGGCCTGGTCTGGACACAGTGCCCGTGTCGGAGCCGCGCGAGATATGGCCCGCGCTGGAGTTTCAATACCGGAGATCATGCAAGCTGGTGGCTGGACCAATGTAAATATTGTCATGAACTATATCCGTAACCTGGATAGTCTAAATGCCGCGATTCCTACTGAGACCAGTCTCTATGGACCGGACCAGACCTGTGTCACGGGCACAGCCTCGGCGCGCTCTATACCGGGCGCGACCTCAAAGTTATGGCCTCTAGTACGTTCGACCACCGACCTGGTTACATTTATAACAGTACTTGATATAGGCATTGGACCTATCAPshAIIY280GA282KD284DS286GQ288RY290LA292WS294GH296SA298RV300GA302AR304DM306AR308AG310VS312IP314EI316MQ318AG320GW322TN324VN326IV328MN330YI332RN334LD336S6644-7695Cre7,4807,5007,5207,5407,5607,5807,6007,6207,640
    GAAACAGGGGCAATGGTGCGCCTGCTGGAAGATGGCGATTAGCCATGTTTAAGGGTTCCTCTAGtgtacACGCGTtgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaCTTTGTCCCCGTTACCACGCGGACGACCTTCTACCGCTAATCGGTACAAATTCCCAAGGAGATCacatgTGCGCAacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactccttMluIE338TG340AM342VR344LL346ED348GD3506644-7695CreBGH polyA7,6607,6807,7007,7207,7407,7607,7807,8007,820
    attgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggataacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctBbsIBGH polyA7,8407,8607,8807,9007,920
    • Plasmid
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    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    7936 bp5500600065007000750050010001500AmpRCre6644-7695ITR,MluI,BglII,MscI,SalI,XbaI,BamHI,Acc65I,KpnI,FspINgoMIV,NaeIRep_OriAloIZraIAatIIXmnIFspINmeAIIIU6 promotergmKras,Chimeric RNA (& trRNA)AscI,PacI,EagI,NotI,BsmIAgeIPGK promoter,BseRI,SpeIBlpI,StuIBspEI,BsmI,BseRI,KflIAgeIXmnI,NruIRsrII,BamHIBstBI,SexAI,AsiSIPaqCI,BspDI,ClaI,PshAIBGH polyA,MluI,BbsI

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    7936