Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
AAV-KrasG12V/sgKras/Cre
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    ACGCGTgatatcagatctgttacgtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagTGCGCActatagtctagacaatgcatctattcatcgtaccgcccaattagtaattgatgttccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcMluIMscIBglIIITR20406080100120140160
    cgagcgagcgcgcagctgcctgcaggtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccggctcgctcgcgcgtcgacggacgtccagctgagatctcctaggggcccatggctcgagcttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcKpnISalIXbaIBamHIAcc65I180200220240260280300320340
    caccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgagtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactFspIRep_Ori360380400420440460480500520
    ccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgggcgatgtgaacggtcgcggaatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcNaeINgoMIVRep_Ori540560580600620640660680700
    tagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgattatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgagatagagcccgataagaaaactaaatattccctaaaacggctaaagccagataaccaattttttactcgactaaAloIRep_Ori720740760780800820840860
    taacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacRep_Ori8809009209409609801,0001,0201,040
    tgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaaZraIAatII1,0601,0801,1001,1201,1401,1601,1801,2001,220
    atttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttAmpR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaaccattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgXmnIAmpR1,4201,4401,4601,4801,5001,5201,5401,560
    tcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaacagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattgAmpR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattagcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaatFspIAmpR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggatatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctBsaINmeAIIIAmpR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagtattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaacagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcataaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttAmpR2,1202,1402,1602,1802,2002,2202,2402,260
    aatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaagttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttcRep-origin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagaccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgRep-origin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccRep-origin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    aacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccRep-origin2,8202,8402,8602,8802,9002,9202,9402,960
    ccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggcggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccgSapIBspQI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacaggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtc3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctatgaccatgattacgccaagcttgcatgcctgcaggcagctgcgcgctcgaacttcatgcctgccgaccttccccaggtcacgatccggacggcgggtgagttcacattttarcagccggacgtgcaractccgctggtggtctaacgtcggttaggtcccttgaatcacggggatactggtactaatgcggttcgaacgtacggacgtccgtcgacgcgcgagcttgaagtacggacggctggaaggggtccagtgctaggcctgccgcccactcaagtgtaaaatygtcggcctgcacgtytgaggcgaccaccagattgcagccaatccagggaacttagtgcccBspEIEcoNIHindIII3,3403,3603,3803,4003,4203,4403,4603,4803,500
    acatatgttggtgttggaggtcctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctggaggggtggagtcgtgacgtaaatgtatacaaccacaacctccaggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacctccccacctcagcactgcatttMscINdeIITR3,5203,5403,5603,5803,6003,6203,6403,660
    gatctgatatcatcgatcgcgatgcattaattaagcggccgccatggCAGTTCTTTTGTATCCATTTGTCTCTTTATCTGCAATAATATATGTTCTACAAGGGAAAGGAGATAAAGTGTATATATTAATTTTATTTCAAAAATGTTTGGAAAAGCAGACTAGGAAACAACAGTGTctagactatagtagctagcgctacgtaattaattcgccggcggtaccGTCAAGAAAACATAGGTAAACAGAGAAATAGACGTTATTATATACAAGATGTTCCCTTTCCTCTATTTCACATATATAATTAAAATAAAGTTTTTACAAACCTTTTCGTCTGATCCTTTGTTGTCACABtgIClaIEagINcoINotINruINsiIPacIBglIIBspDIKrasHR 2.1kb3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TGTATTCAGGGCTGTTAACTGTATACAGTGCCAGCACTTAGTGCACCTGTTGGGAGTGCAGTGTATTATAAGCAAGGCAGAAGTCACAGAGGTTACTGAGAAACTCTTTTATGTTCTTTACATTTTTTTTCTTGCCAAAAATAGTCTTTATCTCTGAATTTCATTCAATTTTATTACATAAGTCCCGACAATTGACATATGTCACGGTCGTGAATCACGTGGACAACCCTCACGTCACATAATATTCGTTCCGTCTTCAGTGTCTCCAATGACTCTTTGAGAAAATACAAGAAATGTAAAAAAAAGAACGGTTTTTATCAGAAATAGAGACTTAAAGTAAGTTAAAATAAHpaIBstAPIBstZ17IKrasHR 2.1kb3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TCCTTGTACAGAGGTACTGTTTTTAGTACTCAAAGGATTATATGTTAAAATTGATCTGATCCTATACCAAATATTTAAACCCATTTCCCACAGATAGTTTATAGATTAAAATGCCATCAAATATTATCTTTATTTGCTTCATTGCCTAAATATGTCTTTCCCCAGCACAGTGCAGAGGAACATGTCTCCATGACAAAAATCATGAGTTTCCTAATATACAATTTTAACTAGACTAGGATATGGTTTATAAATTTGGGTAAAGGGTGTCTATCAAATATCTAATTTTACGGTAGTTTATAATAGAAATAAACGAAGTAACGGATTTATACAGAAAGGGGTCGTGTCACGTCKrasHR 2.1kb4,0404,0604,0804,1004,1204,1404,1604,1804,200
    TTTTGACACCAGCTTCGGCTTCCTATTTTGTTGCCCTTTATTGCAGAACTGCTCTGATGGCTTTAAAACAGTTCTCTGCAGTTGTTGGCTCCAACACAGATGTTCTTAGGCTACCTAACTTCTAACTTTTAATATCCAGTCAACAAAGAATACCGCAAGGGTAGGTGTTGGGATAAAAACTGTGGTCGAAGCCGAAGGATAAAACAACGGGAAATAACGTCTTGACGAGACTACCGAAATTTTGTCAAGAGACGTCAACAACCGAGGTTGTGTCTACAAGAATCCGATGGATTGAAGATTGAAAATTATAGGTCAGTTGTTTCTTATGGCGTTCCCATCCACAACCCTATKrasHR 2.1kb4,2204,2404,2604,2804,3004,3204,3404,360
    GCTGTCGACAAGCTCATGCGGGTGTGTCCACAGGGTATAGCGTACTATGCAGAATATTTGTACTGAGTGAAGTCATGATACATTCCTTTGAGAGCCATTAGCTGCTACAAAACAGTAATCTGGCTGTTTAGATCAACAAGCTAAATGATAGAAGATGAAAGTACTGGTTTCCATGCGACAGCTGTTCGAGTACGCCCACACAGGTGTCCCATATCGCATGATACGTCTTATAAACATGACTCACTTCAGTACTATGTAAGGAAACTCTCGGTAATCGACGATGTTTTGTCATTAGACCGACAAATCTAGTTGTTCGATTTACTATCTTCTACTTTCATGACCAAAGGTACBarISalIKrasHR 2.1kb4,3804,4004,4204,4404,4604,4804,5004,5204,540
    TATTTTTATTAAGTGTTGATGAGAAAGTTGTAAGTGACTTACAGGTTACTCTGTACATCTGTAGTCACTGAATTCGGAATATCTTAGAGTTTTACACACctAGGTGAGTGTTAAAATATTGATAAAGTTTTTGATAATCTTGTGTGAGACATGTTCTAATTTAGTTGTATTTTATATAAAAATAATTCACAACTACTCTTTCAACATTCACTGAATGTCCAATGAGACATGTAGACATCAGTGACTTAAGCCTTATAGAATCTCAAAATGTGTGgaTCCACTCACAATTTTATAACTATTTCAAAAACTATTAGAACACACTCTGTACAAGATTAAATCAACATAAAATAAleIAvrIIKrasHR 2.1kb4,5604,5804,6004,6204,6404,6604,6804,7004,720
    TATTTTTATTGTAAGGCCTGCTGAAAATGACTGAGTATAAACTaGTaGTcGTTGGAGCTGTTGGCGTCGGGAAATCGGCCCTTACTATTCAGCTAATTCAGAATCACTTTGTGGATGAGTATGACCCTACGATAGAGGTAACGCTGCTCTACAGTCTGCGTaCGCTTGTAAAGGAATAAAAATAACATTCCGGACGACTTTTACTGACTCATATTTGAtCAtCAgCAACCTCGACAACCGCAGCCCTTTAGCCGGGAATGATAAGTCGATTAAGTCTTAGTGAAACACCTACTCATACTGGGATGCTATCTCCATTGCGACGAGATGTCAGACGCAtGCGAACATTTCCTSpeIStuIBsiWIM1T2E3Y4K5L6V7V8V9G10A11V12G13V14G15K16S17A18L19T20I21Q22L23I24Q25N26H27F28V29D30E31Y32D33P34T35I36E374752-4863KrasHR 2.1kbexon2BarcodesgKras4,7404,7604,7804,8004,8204,8404,8604,8804,900
    CGGCAGCCAGCCGCTTTGAAAAAGATATCATTTTTATATTTATTAGAAAATTATATTGAAAGTTATTTCAGTTATATGTGATGTCCTTTAGTTCCAAGGCTTTAAACTGGGTGTTAGGGAACCATAGGTGCAAGAAAGTCCACTTCTCATGAGAGCTCACCACAGAGAAAGAAAGGCCGTCGGTCGGCGAAACTTTTTCTATAGTAAAAATATAAATAATCTTTTAATATAACTTTCAATAAAGTCAATATACACTACAGGAAATCAAGGTTCCGAAATTTGACCCACAATCCCTTGGTATCCACGTTCTTTCAGGTGAAGAGTACTCTCGAGTGGTGTCTCTTTCTTTCKrasHR 2.1kb4,9204,9404,9604,9805,0005,0205,0405,060
    TCCACTTCTCATGAGAGCTTACCACAGAGAGTAAGGTCCAAGATGCCATCCGTGCTTTGTATTTGCATTTTGATAAATAAATTTCATGATTTTGTGAGAGTTAAGAATATAGCTTTGCCTTTGATCTATTCCTGAAAGGTTTGTTGTTGTTTTATGGGTTTGTTGTTATTGTGTTAGGTGAAGAGTACTCTCGAATGGTGTCTCTCATTCCAGGTTCTACGGTAGGCACGAAACATAAACGTAAAACTATTTATTTAAAGTACTAAAACACTCTCAATTCTTATATCGAAACGGAAACTAGATAAGGACTTTCCAAACAACAACAAAATACCCAAACAACAATAACACAAKrasHR 2.1kb5,0805,1005,1205,1405,1605,1805,2005,2205,240
    GTTGTTTGGTGTCTTTTAGGCAGGGTCTTACTATGCAGCCCTGACCGGCCTAGAACTCAACTATGTAGACCAGACTAGCTCTGAATTCTCAGAGATCCATCTACCTGCCTCTGCTGCCCAGATGCTGCGATTAAAGATGTACACCACCATGTACAGTGAAAGTTACTCTTTTGAACAACAAACCACAGAAAATCCGTCCCAGAATGATACGTCGGGACTGGCCGGATCTTGAGTTGATACATCTGGTCTGATCGAGACTTAAGAGTCTCTAGGTAGATGGACGGAGACGACGGGTCTACGACGCTAATTTCTACATGTGGTGGTACATGTCACTTTCAATGAGAAAACTTKrasHR 2.1kb5,2605,2805,3005,3205,3405,3605,3805,4005,420
    AACACTTTATTGTTATAGATTACTTGGAATGTTGATGTGATGAGAAAATTTGATTATACTAGGAAAATAGAATCACTTGATGGTTGAGCTTTTTTTTTAAGACTTATTTATTTTATGTAAGTGAGTACACTGCTTCTCTCTTCAGACACACCAGAAGAGGGCATCAGATCCCATTTTGTGAAATAACAATATCTAATGAACCTTACAACTACACTACTCTTTTAAACTAATATGATCCTTTTATCTTAGTGAACTACCAACTCGAAAAAAAAATTCTGAATAAATAAAATACATTCACTCATGTGACGAAGAGAGAAGTCTGTGTGGTCTTCTCCCGTAGTCTAGGGTAAKrasHR 2.1kb5,4405,4605,4805,5005,5205,5405,5605,5805,600
    AAGATGGCTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAAGGCAGTCAGTGCTCTTTTTTTGTTTAAAGATTTATTTATTTTTATGAATACAGTGTCACTGTCTTCATACACACAAGAGctcgaGCTGTGAGGGCCTATTTCCCATGATTCCTTCATATTCTACCGACACTCGGTGGTACACCAACGACCCTTAACTTGAGTCCTGGAGACCTTTCCGTCAGTCACGAGAAAAAAACAAATTTCTAAATAAATAAAAATACTTATGTCACAGTGACAGAAGTATGTGTGTTCTCgagctCGACACTCCCGGATAAAGGGTACTAAGGAAGTATBbsITliIXcmIXhoIBstXIPflMIPspXIBstAPIPaeR7IKrasHR 2.1kbU6 promoter5,6205,6405,6605,6805,7005,7205,7405,760
    TTTGCATATACGATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAACCTTAATTAAACTGACATTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATTAAAGAACCCATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAACTTTCATANdeIU6 promoter5,7805,8005,8205,8405,8605,8805,9005,9205,940
    TTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACCAAACACCGACTGAGTATAAACTTGTGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTGTTTGGCGCGCCTATGGCTTAATTAACAAAGCTAAAGAACCGAAATATATAGAACACCTTTCCTGGTTTGTGGCTGACTCATATTTGAACACCCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGAACTTTTTCACCGTGGCTCAGCCACGAAAAAACAAACCGCGCGGATACCGAATTAATTGTAscIPacIChimeric RNA (& trRNA)U6 promotergmKras5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TGGCGGCCGCATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCGCCGGCGTAAGATGGCCCATCCCCTCCGCGAAAAGGGTTCCGTCAGACCTCGTACGCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGAGACCGGAGCGTGTGTAAGGTGTAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCGGAgeIBsmIEagINotIPGK promoter6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCTGGAAGATGAGGAGGGGATCAGTCCTTCAAGGGGGGGCGGGGCGTCGAGCGCAGCACGTCCTGCACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTGTCGTGGCGACTCGTTACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGBlpISpeIStuIBseRIPGK promoter6,3206,3406,3606,3806,4006,4206,4406,460
    GCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGACCCGGGATGCCCACGAAAGACCCGAGTCTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCCCGCCCCGCCCGCGGGCTTCCAGGAGGCCTCCGGGCCGTAAGACGTGCGAAGTTTTCGCGTGCAGACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCTGGGCCCTACGGGTBsmIKflIBseRIBspEIMP2PGK promoter6,4806,5006,5206,5406,5606,5806,6006,6206,640
    AGAAGAAGAGGAAGGTGTCCAATTTACTGACCGTACACCAAAATTTGCCTGCATTACCGGTCGATGCAACGAGTGATGAGGTTCGCAAGAACCTGATGGACATGTTCAGGGATCGCCAGGCGTTTTCTGAGCATACCTGGAAAATGCTTCTGTCCGTTTGCCGGTCGTGGGCGGCTCTTCTTCTCCTTCCACAGGTTAAATGACTGGCATGTGGTTTTAAACGGACGTAATGGCCAGCTACGTTGCTCACTACTCCAAGCGTTCTTGGACTACCTGTACAAGTCCCTAGCGGTCCGCAAAAGACTCGTATGGACCTTTTACGAAGACAGGCAAACGGCCAGCACCCGCCGAgeIKK4KR6KV8SN10LL12TV14HQ16NL18PA20LP22VD24AT26SD28EV30RK32NL34MD36MF38RD40RQ42AF44SE46HT48WK50ML52LS54VC56RS58WA60A6644-7695Cre6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ATGGTGCAAGTTGAATAACCGGAAATGGTTTCCCGCAGAACCTGAAGATGTTCGCGATTATCTTCTATATCTTCAGGCGCGCGGTCTGGCAGTAAAAACTATCCAGCAACATTTGGGCCAGCTAAACATGCTTCATCGTCGGTCCGGGCTGCCACGACCAAGTGACAGCAATGCTTACCACGTTCAACTTATTGGCCTTTACCAAAGGGCGTCTTGGACTTCTACAAGCGCTAATAGAAGATATAGAAGTCCGCGCGCCAGACCGTCATTTTTGATAGGTCGTTGTAAACCCGGTCGATTTGTACGAAGTAGCAGCCAGGCCCGACGGTGCTGGTTCACTGTCGTTACGANruIXmnIRsrIIW62CK64LN66NR68KW70FP72AE74PE76DV78RD80YL82LY84LQ86AR88GL90AV92KT94IQ96QH98LG100QL102NM104LH106RR108SG110LP112RP114SD116SN118A6644-7695Cre6,8406,8606,8806,9006,9206,9406,9606,9807,000
    GTTTCACTGGTTATGCGGCGGATCCGAAAAGAAAACGTTGATGCCGGTGAACGTGCAAAACAGGCTCTAGCGTTCGAACGCACTGATTTCGACCAGGTTCGTTCACTCATGGAAAATAGCGATCGCTGCCAGGATATACGTAATCTGGCATTTCTGGGGATTGCTTATAACACCCCAAAGTGACCAATACGCCGCCTAGGCTTTTCTTTTGCAACTACGGCCACTTGCACGTTTTGTCCGAGATCGCAAGCTTGCGTGACTAAAGCTGGTCCAAGCAAGTGAGTACCTTTTATCGCTAGCGACGGTCCTATATGCATTAGACCGTAAAGACCCCTAACGAATATTGTGGGAsiSIBamHIBstBISexAIV120SL122VM124RR126IR128KE130NV132DA134GE136RA138KQ140AL142AF144ER146TD148FD150QV152RS154LM156EN158SD160RC162QD164IR166NL168AF170LG172IA174YN176T6644-7695Cre7,0207,0407,0607,0807,1007,1207,1407,160
    TGTTACGTATAGCCGAAATTGCCAGGATCAGGGTTAAAGATATCTCACGTACTGACGGTGGGAGAATGTTAATCCATATTGGCAGAACGAAAACGCTGGTTAGCACCGCAGGTGTAGAGAAGGCACTTAGCCTGGGGGTAACTAAACTGGTCGAGCGATGGATTTCCGTCTCTGGACAATGCATATCGGCTTTAACGGTCCTAGTCCCAATTTCTATAGAGTGCATGACTGCCACCCTCTTACAATTAGGTATAACCGTCTTGCTTTTGCGACCAATCGTGGCGTCCACATCTCTTCCGTGAATCGGACCCCCATTGATTTGACCAGCTCGCTACCTAAAGGCAGAGACCPaqCIL178LR180IA182EI184AR186IR188VK190DI192SR194TD196GG198RM200LI202HI204GR206TK208TL210VS212TA214GV216EK218AL220SL222GV224TK226LV228ER230WI232SV234SG2366644-7695Cre7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TGTAGCTGATGATCCGAATAACTACCTGTTTTGCCGGGTCAGAAAAAATGGTGTTGCCGCGCCATCTGCCACCAGCCAGCTATCAACTCGCGCCCTGGAAGGGATTTTTGAAGCAACTCATCGATTGATTTACGGCGCTAAGGATGACTCTGGTCAGAGATACCTGGCCTGGTCTACATCGACTACTAGGCTTATTGATGGACAAAACGGCCCAGTCTTTTTTACCACAACGGCGCGGTAGACGGTGGTCGGTCGATAGTTGAGCGCGGGACCTTCCCTAAAAACTTCGTTGAGTAGCTAACTAAATGCCGCGATTCCTACTGAGACCAGTCTCTATGGACCGGACCAGAClaIBspDIPshAIVA238DD240PN242NY244LF246CR248VR250KN252GV254AA256PS258AT260SQ262LS264TR266AL268EG270IF272EA274TH276RL278IY280GA282KD284DS286GQ288RY290LA292WS2946644-7695Cre7,3607,3807,4007,4207,4407,4607,4807,5007,520
    GGACACAGTGCCCGTGTCGGAGCCGCGCGAGATATGGCCCGCGCTGGAGTTTCAATACCGGAGATCATGCAAGCTGGTGGCTGGACCAATGTAAATATTGTCATGAACTATATCCGTAACCTGGATAGTGAAACAGGGGCAATGGTGCGCCTGCTGGAAGATGGCGATTAGCCATCCTGTGTCACGGGCACAGCCTCGGCGCGCTCTATACCGGGCGCGACCTCAAAGTTATGGCCTCTAGTACGTTCGACCACCGACCTGGTTACATTTATAACAGTACTTGATATAGGCATTGGACCTATCACTTTGTCCCCGTTACCACGCGGACGACCTTCTACCGCTAATCGGTAGH296SA298RV300GA302AR304DM306AR308AG310VS312IP314EI316MQ318AG320GW322TN324VN326IV328MN330YI332RN334LD336SE338TG340AM342VR344LL346ED348GD3506644-7695Cre7,5407,5607,5807,6007,6207,6407,6607,6807,700
    GTTTAAGGGTTCCTCTAGtgtacACGCGTtgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctgggggCAAATTCCCAAGGAGATCacatgTGCGCAacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagacccccMluIBGH polyA7,7207,7407,7607,7807,8007,8207,8407,860
    gtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctBbsIBGH polyA7,8807,9007,920
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    7936 bp5500600065007000750050010001500AmpRCre6644-7695

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    7936