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EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF
  • Sequence Map
    Editing disabled on read-only sequence.
    aagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccattctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggt20406080100120140160
    atgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaactacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttgAhdIBsaIAmp180200220240260280300320340
    cagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttgtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaaAclIFspIAmp360380400420440460480500520
    cattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaggtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattcAmp540560580600620640660680700
    atgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttatacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatAclIBcgIScaIAmp720740760780800820840860
    ccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataaAmp8809009209409609801,0001,0201,040
    attgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttttaacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaa1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctttggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggaPsiI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaacttagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattg1,4201,4401,4601,4801,5001,5201,5401,560
    caccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcagtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtFspI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatattggctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataacBssHIIBII(R)5'TR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttgBsiWI31bp spacer5'TR(1)5'TR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatcccagagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtagggtctcggccgtcgcacgcccccore insul2,1202,1402,1602,1802,2002,2202,2402,260
    acagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgacgattgagtAAACAGGAAATATATAAAGAGctcttcAAACAGGAAATATATAAAGAtgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaactgctaactcaTTTGTCCTTTATATATTTCTCgagaagTTTGTCCTTTATATATTTCTMfeISfiISrfIcore insulZNF35/IKZF1 siteZNF35/I...1 site2,2802,3002,3202,3402,3602,3802,4002,4202,440
    GgaggtcAAACAGGAAATATATAAAGAGcagaacAAACAGGAAATATATAAAGAGagtctagagggtatataatggatcccatcgcgtctcagcctcactttgagctcctccacacgaattccccgataccgtcgattcaaggagcttgcttgttctttttgcagaagctcagaaCctccagTTTGTCCTTTATATATTTCTCgtcttgTTTGTCCTTTATATATTTCTCtcagatctcccatatattacctagggtagcgcagagtcggagtgaaactcgaggaggtgtgcttaaggggctatggcagctaagttcctcgaacgaacaagaaaaacgtcttcgagtcttXbaIXenopus globin 5'-UTRZNF35/IKZF1 siteZNF35/IKZF1 site2,4602,4802,5002,5202,5402,5602,5802,6002,620
    taaacgctcaactttggcagatctaccaagcttggtaccgagctcggatccaactcgaggccaccatgccagagccagcgaagtctgctcccgccccgaaaaagggctccaagaaggcggtgactaaggcgcagaagaaaggcggcaagaagcgcaagcgcagccgcaaggagagatttgcgagttgaaaccgtctagatggttcgaaccatggctcgagcctaggttgagctccggtggtacggtctcggtcgcttcagacgagggcggggctttttcccgaggttcttccgccactgattccgcgtcttctttccgccgttcttcgcgttcgcgtcggcgttcctctcKpnITliIXhoIBglIIPspXIAcc65IPaeR7IMP2EP4AK6SA8PA10PK12KG14SK16KA18VT20KA22QK24KG26GK28KR30KR32SR34KE36SH2B-BFPH2BXenop...-UTR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ctattccatctatgtgtacaaggttctgaagcaggtccaccctgacaccggcatttcgtccaaggccatgggcatcatgaattcgtttgtgaacgacattttcgagcgcatcgcaggtgaggcttcccgcctggcgcattacaacaagcgctcgaccatcacctccagggagatcgataaggtagatacacatgttccaagacttcgtccaggtgggactgtggccgtaaagcaggttccggtacccgtagtacttaagcaaacacttgctgtaaaagctcgcgtagcgtccactccgaagggcggaccgcgtaatgttgttcgcgagctggtagtggaggtccctctagAfeIPaqCIY38SI40YV42YK44VL46KQ48VH50PD52TG54IS56SK58AM60GI62MN64SF66VN68DI70FE72RI74AG76EA78SR80LA82HY84NK86RS88TI90TS92RE94IH2B-BFPH2B2,8202,8402,8602,8802,9002,9202,9402,960
    cagacggccgtgcgcctgctgctgcctggggagttggccaagcacgccgtgtccgagggtactaaggccatcaccaagtacaccagcgctaaggatccccgggtaccggtcgtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccggtctgccggcacgcggacgacgacggacccctcaaccggttcgtgcggcacaggctcccatgattccggtagtggttcatgtggtcgcgattcctaggggcccatggccagcacagattcccgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtggcAfeIAgeIKpnIAcc65IBpu10IQ96TA98VR100LL102LP104GE106LA108KH110AV112SE114GT116KA118IT120KY122TS124AK126DP128RV130PV132VS134KG136EE138LI140KE142NM144HM146KL148YM150EG152TH2B-BFPH2BmtagBFP2linker2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatcccacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtagggV154DN156HH158FK160CT162SE164GE166GK168PY170EG172TQ174TM176RI178KV180VE182GG184PL186PF188AF190DI192LA194TS196FL198YG200SK202TF204IN206HT208QG210IP212H2B-BFPmtagBFP23,1603,1803,2003,2203,2403,2603,2803,3003,320
    cgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagaaggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtcttcPfoIBsu36IDF214FK216QS218FP220EG222FT224WE226RV228TT230YE232DG234GV236LT238AT240QD242TS244LQ246DG248CL250IY252NV254KI256RG258VN260FT262SN264GP266VM268QK270H2B-BFPmtagBFP23,3403,3603,3803,4003,4203,4403,4603,4803,500
    aaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctactttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagatgaKT272LG274WE276AF278TE280TL282YP284AD286GG288LE290GR292ND294MA296LK298LV300GG302SH304LI306AN308AK310TT312YR314SK316KP318AK320NL322KM324PG326VY328H2B-BFPmtagBFP23,5203,5403,5603,5803,6003,6203,6403,660
    atgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaagcttaattagctagcgaattcgaatttaaatcggcctcgactgtgccttctagttgccagccatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgttcgaattaatcgatcgcttaagcttaaatttagccggagctgacacggaagatcaacggtcggtBmtINheISwaIBstBIYV330DY332RL334ER336IK338EA340NN342ET344YV346EQ348HE350VA352VA354RY356CD358LP360SK362LG364HK366LN368*H2B-BFPmtagBFP2BGH polya...sequenceMCS3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgBGH polyadenylation sequence3,8603,8803,9003,9203,9403,9603,9804,0004,020
    atgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcatacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtNotISexAIpSV405, 299 FBG...e4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgpSV404,2204,2404,2604,2804,3004,3204,3404,360
    ttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaataaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcactactttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttaBclIPmlIAvrIIPflFIPshAITth111IHygropSV404,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcgagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagHygro2, 563 F4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagAsiSIHygro4,7404,7604,7804,8004,8204,8404,8604,8804,900
    atatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatgtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttacNdeIPflFITth111IHygro4,9204,9404,9604,9805,0005,0205,0405,060
    gccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctcggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaAjuISacIIHygro3, 135 F5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagScaIHygro5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataggtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaaccggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatccagctgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgHpaIWPREHygro6, 730 R5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccaPsiISV40 pASV40 pA(1)5,6205,6405,6605,6805,7005,7205,7405,760
    ctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttcccaaactgggttcttatatcccttgctctggtcaaccaggttgcagggtttcctgtcctcacaggaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattcgagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaagggtttgacccaagaatatagggaacgagaccagttggtccaacgtcccaaaggacaggagtgtccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaagBsaISexAI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggctcaaagagcagcgagaagcgttcagaggaaagcgatcccgtgccaccttccccgtgcccgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcgggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtccgagtttctcgtcgctcttcgcaagtctcctttcgctagggcacggtggaaggggcacgggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgccApaISrfIEcoNIPspOMIcore insul(1) Geneious name: core insul(1)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    agccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggggctttgggggggggctgtccctgatgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataactcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccccgaaacccccccccgacagggactacaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgSpeICMVcore insul(1) Geneious name: core insul(1)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgNdeICMV6,3206,3406,3606,3806,4006,4206,4406,460
    gccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggacgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctSnaBICMV6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctaaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatBglIIBsaBICMVTetR binding sitesTetO6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgTACGAGTGTAATGAGTGCGGTAAGACCTagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacATGCTCACATTACTCACGCCATTCTGGAPmeIAflIIBsaBISgrDIMY2EC4NE6CG8KT10ZNF35/IKZF1 TFZNF35 ZF Domains 5-8CMVTet...esTetO6,8406,8606,8806,9006,9206,9406,9606,9807,000
    TCACACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTGGGAAGGCTTTTACTCAGAGTGCCAACTTGATCGTGCATCAGAGAAGCCATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGAGTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACACCCTTCCGAAAATGAGTCTCACGGTTGAACTAGCACGTAGTCTCTTCGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACFT12RS14SN16LI18VH20QR22IH24TG26EK28PF30AC32ND34CG36KA38FT40QS42AN44LI46VH48QR50SH52TG54EK56PY58EC60KE62CG64KA66FS68CZNF35/IKZF1 TFZNF35 ZF Domains 5-87,0207,0407,0607,0807,1007,1207,1407,160
    CTTCAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTTTAGTCAGCTGTCTTGCTTGATTGTGCATCAGAGAATTCACACGGGCGAACGCCCCTTCCAATGCAATCAGTGCGGGGCATCTTTCACTCAGAAGGGGGAAGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAAATCAGTCGACAGAACGAACTAACACGTAGTCTCTTAAGTGTGCCCGCTTGCGGGGAAGGTTACGTTAGTCACGCCCCGTAGAAAGTGAGTCTTCCCCF70SH72LI74VH76QR78IH80TA82EK84PY86DC88SE90CG92KA94FS96QL98SC100LI102VH104QR106IH108TG110ER112PF114QC116NQ118CG120AS122FT124QK126GZNF35/IKZF1 TFZNF35 ZF Domains 5-8IKZF1 ZF 2-4Cano...ker7,1807,2007,2207,2407,2607,2807,3007,3207,340
    AACTTACTGCGCCATATTAAATTGCACTCTGGTGAGAAACCGTTTAAATGTCACTTATGCAACTACGCCTGCAGGCGCAGAGATGCCTTGACCGGCCACCTCAGAACCCATTCAGTCGGCAAACCCCATAAGTGCGGGTACTGTGGCAGATCATATAAACAAAGGAGCTCCCTGGTTGAATGACGCGGTATAATTTAACGTGAGACCACTCTTTGGCAAATTTACAGTGAATACGTTGATGCGGACGTCCGCGTCTCTACGGAACTGGCCGGTGGAGTCTTGGGTAAGTCAGCCGTTTGGGGTATTCACGCCCATGACACCGTCTAGTATATTTGTTTCCTCGAGGGACCAleISbfIN128LL130RH132IK134LH136SG138EK140PF142KC144HL146CN148YA150CR152RR154DA156LT158GH160LR162TH164SV166GK168PH170KC172GY174CG176RS178YK180QR182SS184LZNF35/IKZF1 TFIKZF1 ZF 2-47,3607,3807,4007,4207,4407,4607,4807,5007,520
    AAGAACATAAGGAAAGGTGTCACgctgctgcaggcTGGagcggaggcGATggcggaGACGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCTGGTGAATTCTTGTATTCCTTTCCACAGTGcgacgacgtccgACCtcgcctccgCTAccgcctCTGCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCGACCACTTBlpIBsgIE186EH188KE190RC192HA194AA196GW198SG200GD202GG204DE206GQ208SD210ER212AL214LD216QL218HT220LL222SN224TD226AT228GL230EE232ID234RA236LG238IP240EL242VN244ZNF35/IKZF1 TFN (NCOA3) Activation DomainNZF DomainIKZF1 ZF 2-47,5407,5607,5807,6007,6207,6407,6607,6807,700
    CCAGGGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGACCTGTTCGGTCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCTAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCTGGACAAGBmgBIEcoNIPflMIPpuMIQG246QA248LE250PK252QD254GG256WG258SM260AE262EF264VT266LK268DV270GM272DF274TL276GD278WE280QL282GL284EQ286GD288TF290WD292TA294LD296NC298QD300LF302ZNF35/IKZF1 TFNZF DomainZ (ZNF473) Activation DomainN (NCOA3) Activation Domain7,7207,7407,7607,7807,8007,8207,8407,860
    CTGCTGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGACACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCtaggccgctcGACGACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGatccggcgagPciITliIXhoIPspXIAflIIIPaeR7ILL304DP306PG308GG310DG312LG314LG316HE318KF320PS322DL324DL326DM328FN330GS332LE334CD336ME338SI340IR342SE344LM346DA348DG350LD352FN354FD356S*358ZNF35/IKZF1 TFNZF DomainF (FOXO3) Activation DomainZ (Z...ain7,8807,9007,9207,9407,9607,9808,0008,0208,040
    gaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccacctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgApaIBclIPmeIPspOMIBGH polyadenylation sequencec-myc epitopePolyhi...e tag8,0608,0808,1008,1208,1408,1608,1808,2008,220
    tcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggaatctataacaagaaaatatatatataataagttatcacgtagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgaccccttagatattgttcttttatatatatattattcaatagtgcaBGH polyadenylation sequence8,2408,2608,2808,3008,3208,3408,3608,3808,400
    aagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggat8,4208,4408,4608,4808,5008,5208,5408,560
    aatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcgggttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagccc3'TR8,5808,6008,6208,6408,6608,6808,7008,7208,740
    gaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggatactccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctat8,7608,7808,8008,8208,8408,8608,8808,9008,920
    ccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcaggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtBsgI8,9408,9608,9809,0009,0209,0409,0609,0809,100
    gaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacBssHIIpLac(F)9,1209,1409,1609,1809,2009,2209,2409,260
    cgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaagcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagttt9,2809,3009,3209,3409,3609,3809,4009,4209,440
    ggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgPciIAflIII9,4609,4809,5009,5209,5409,5609,5809,6009,620
    ccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggt9,6409,6609,6809,7009,7209,7409,7609,7809,800
    agctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtgtcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccac9,8209,8409,8609,8809,9009,9209,9409,960
    gcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagcggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttc9,98010,00010,02010,04010,06010,08010,10010,12010,140
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10148 bp750080008500900095005001000150020002500ZNF35/IKZF1 siteGibson overlapMluI-PGK-GFNew StopZNF35/IKZF1 TFEWo146.2 (DNA)EWo153.4 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),AhdI,BsaI,FspI,AclIAmp,ScaI,BcgIAclIPsiIFrtF (DNA)FlpInR (DNA),FspIM13-40FOR Genewiz (DNA),M13F Genewiz (DNA),BssHIIBII(R),5'TRBsiWI31bp spacer,5'TR(1),5'TR,5'TRcore insul,SrfIAdded MluI site,ZNF35/IKZF1 site,+4ZNF35/IKZF1 site,ZNF35/IKZF1 site,EWo122.1 (DNA),+3EWXglobR (DNA)EWo195.2 (DNA),EWo195.1 (DNA)N (NCOA3) Activation Domain,EWo183 (DNA),EW_NZF_R (DNA),+2NZF Domain,Z (ZNF473) Activation Domain,BmgBI,EcoNIPpuMI,AflIII,PciIF (FOXO3) Activation Domain,c-myc epitope,PaeR7I,PspXI,+4Polyhistidine tag,STOP (missing now),ToFlpR (DNA),+3BGH polyadenylation sequence,BghR1 (DNA),bghF (DNA)EWo370.2 (DNA)3'TRES003-3 C->ABsgIpLac(F),M13R Genewiz (DNA),BssHIIpUCori5F (DNA)pUCoriR (DNA),AflIII,PciI

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    10148