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APL0043 SFFV-CCR6-ADAR2dd (FLP-IN) from xz032/APL31
  • Sequence Map
    Editing disabled on read-only sequence.
    gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatag*W286HK284IL282SA280GI278145-1005Amp (bla)20406080100120140160
    tcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtAhdIBglIBsaIEA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SR220L145-1005Amp (bla)180200220240260280300320340
    gaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatccttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagAclIFspIL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170EN168LE166PE164WR162D145-1005Amp (bla)360380400420440460480500520
    aaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtacttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgPvuIL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104Y145-1005Amp (bla)540560580600620640660680700
    tcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacaAclIXmnIE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44145-1005Amp (bla)720740760780800820840860
    aacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgttgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacSspIGV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M145-1005Amp (bla)bla promoter8809009209409609801,0001,0201,040
    tctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgcagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgAlwNIBglIISgrDIbla promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaagctagctgcagtaacgccagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaataccgttcgatcgacgtcattgcggBmtIMfeINheISFFV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    attattgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccgcagtttcggccccggcccgaggccataataacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggcgtcaaagccggggccgggctccggtSmaIXmaIEcoRVTspMIBstEIISFFV1,4201,4401,4601,4801,5001,5201,5401,560
    agagcagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttctgcttcccgagctctataaaagagcttctcgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagacgaagggctcgagatattttctcgaSacIAflIIBbvCIBpu10IBssHIIEco53kIEco53kISFFV1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGAGCGGAGAGAGCATGAACTTCAGTGATGTGTTCGATTCCAGTGAGGACTACTTCGTGTCCGTCAATACATCCTACTACAGCGTAGATTCTGAGATGCTGCTTTGCTCCgtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACTCGCCTCTCTCGTACTTGAAGTCACTACACAAGCTAAGGTCACTCCTGATGAAGCACAGGCAGTTATGTAGGATGATGTCGCATCTAAGACTCTACGACGAAACGAGGAscISacISmaIXmaITspMIBssHIIHindIIICCR6SFFV1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CTGCAGGAGGTGCGCCAGTTCTCCCGACTCTTCGTTCCCATCGCGTATTCACTCATCTGCGTCTTTGGCCTCCTGGGGAATATCTTGGTAGTCATCACATTTGCTTTTTATAAAAAGGCCAGATCCATGACAGACGTCTACCTGCTTAATATGGCCATTGCAGACATCCTGTTCGGACGTCCTCCACGCGGTCAAGAGGGCTGAGAAGCAAGGGTAGCGCATAAGTGAGTAGACGCAGAAACCGGAGGACCCCTTATAGAACCATCAGTAGTGTAAACGAAAAATATTTTTCCGGTCTAGGTACTGTCTGCAGATGGACGAATTATACCGGTAACGTCTGTAGGACAAGCMscIPsiISbfIEcoNICCR61,9401,9601,9802,0002,0202,0402,0602,0802,100
    TCCTGACGCTCCCATTCTGGGCTGTGAGTCACGCTACCGGGGCCTGGGTGTTCAGTAATGCAACCTGCAAGCTGTTGAAGGGAATTTATGCCATCAACTTTAATTGCGGTATGCTGCTGCTGACTTGCATAAGTATGGACAGATACATCGCCATAGTACAGGCAACAAAATCCTTAGGACTGCGAGGGTAAGACCCGACACTCAGTGCGATGGCCCCGGACCCACAAGTCATTACGTTGGACGTTCGACAACTTCCCTTAAATACGGTAGTTGAAATTAACGCCATACGACGACGACTGAACGTATTCATACCTGTCTATGTAGCGGTATCATGTCCGTTGTTTTAGGAACCR62,1202,1402,1602,1802,2002,2202,2402,260
    CAGACTGAGGTCTCGAACGCTCCCTAGGTCTAAAATCATTTGTCTTGTTGTATGGGGCTTGAGCGTGATTATTTCCAGCAGTACTTTCGTGTTTAACCAGAAGTACAACACGCAGGGCAGCGATGTGTGTGAGCCCAAGTACCAGACGGTATCTGAGCCTATCAGGTGGAAGCTGGTCTGACTCCAGAGCTTGCGAGGGATCCAGATTTTAGTAAACAGAACAACATACCCCGAACTCGCACTAATAAAGGTCGTCATGAAAGCACAAATTGGTCTTCATGTTGTGCGTCCCGTCGCTACACACACTCGGGTTCATGGTCTGCCATAGACTCGGATAGTCCACCTTCGACBsaIAvrIICCR62,2802,3002,3202,3402,3602,3802,4002,4202,440
    TTGATGCTGGGTTTGGAATTGCTGTTCGGCTTTTTTATCCCACTCATGTTTATGATTTTTTGTTATACCTTCATCGTGAAGACCCTGGTCCAGGCACAAAACTCTAAGCGACACAAGGCCATCCGGGTAATCATCGCTGTTGTGCTGGTATTTCTGGCTTGCCAGATCCCTCATAAACTACGACCCAAACCTTAACGACAAGCCGAAAAAATAGGGTGAGTACAAATACTAAAAAACAATATGGAAGTAGCACTTCTGGGACCAGGTCCGTGTTTTGAGATTCGCTGTGTTCCGGTAGGCCCATTAGTAGCGACAACACGACCATAAAGACCGAACGGTCTAGGGAGTATAloIEcoNICCR62,4602,4802,5002,5202,5402,5602,5802,6002,620
    ATATGGTCCTTCTGGTAACGGCAGCCAACCTGGGCAAAATGAATCGCTCATGTCAGTCAGAAAAGCTTATCGGGTACACCAAAACGGTTACGGAGGTTCTGGCGTTCCTGCATTGTTGCCTTAACCCTGTGCTGTACGCATTTATAGGTCAGAAGTTTCGAAACTACTTCCTGAATATACCAGGAAGACCATTGCCGTCGGTTGGACCCGTTTTACTTAGCGAGTACAGTCAGTCTTTTCGAATAGCCCATGTGGTTTTGCCAATGCCTCCAAGACCGCAAGGACGTAACAACGGAATTGGGACACGACATGCGTAAATATCCAGTCTTCAAAGCTTTGATGAAGGACTTBstBIHindIIICCR62,6402,6602,6802,7002,7202,7402,7602,7802,800
    GATTCTCAAAGACCTCTGGTGTGTAAGAAGAAAGTATAAAAGTAGTGGATTTTCATGCGCAGGACGCTACTCCGAAAACATTTCCAGACAGACCAGTGAGACGGCCGATAACGATAACGCCTCCTCCTTTACCATGGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCTAAGAGTTTCTGGAGACCACACATTCTTCTTTCATATTTTCATCACCTAAAAGTACGCGTCCTGCGATGAGGCTTTTGTAAAGGTCTGTCTGGTCACTCTGCCGGCTATTGCTATTGCGGAGGAGGAAATGGTACCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGAgeIFspIKpnINcoIBsmBIEsp3IAcc65IGT2GA4LH6LD8QT10PS12R2937-4160CCR6hADAR2 cat domainhomolog2,8202,8402,8602,8802,9002,9202,9402,960
    CAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACBstEIIQ14PI16PS18EG20LQ22LH24LP26QV28LA30DA32VS34RL36VL38GK40FG42DL44TD46NF48SS50PH52AR54RK56VL58AG60VV62MT64TG66TD68VK70D2937-4160hADAR2 cat domain2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGBclIA72KV74IS76VS78TG80TK82CI84NG86EY88MS90DR92GL94AL96ND98CH100AE102II104SR106RS108LL110RF112LY114TQ116LE118LY120LN122NK124DD126QK128RS1302937-4160hADAR2 cat domain3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGPfoIBsrGIIF132QK134SE136RG138GF140RL142KE144NV146QF148HL150YI152ST154SP156CG158DA160RI162FS164PH166EP168IL170EE172PA174DR176HP178NR180KA182RG184QL186RT1882937-4160hADAR2 cat domain3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAABlpIBamHIKI190ES192GQ194GT196IP198VR200SN202AS204IQ206AW208DG210VL212QG214ER216LL218TM220SC222SD224KI226AR228WN230VV232GI234QG236SL238LS240IF242VE244PI2462937-4160hADAR2 cat domain3,5203,5403,5603,5803,6003,6203,6403,660
    ACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCTliIXhoIPaeR7IYF248SS250II252LG254SL256YH258GD260HL262SR264AM266YQ268RI270SN272IE274DL276PP278LY280TL282NK284PL286LS288GI290SN292AE294AR296QP298GK300AP302NF304S2937-4160hADAR2 cat domain3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACMluIV306NW308TV310GD312SA314IE316VI318NA320TT322GK324DE326LG328RA330SR332LC334KH336AL338YC340RW342MR344VH346GK348VP350SH352LL354RS356KI358TK360PN362V2937-4160hADAR2 cat domain3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgacgctcgaggagggcccgaacaaaaactcatctcagaagagATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactgcgagctcctcccgggcttgtttttgagtagagtcttctcApaIBglITliIXhoIPspXIPaeR7IPspOMIY364HE366SK368LA370AK372EY374QA376AK378AR380LF382TA384FI386KA388GL390GA392WV394EK396PT398EQ400DQ402FS404LT406P*4082937-4160hADAR2 cat domainc-myc epitope4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacBclIPmeIBGH polyadenylation sequencePolyhi...e tag4,2204,2404,2604,2804,3004,3204,3404,360
    catcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttgClaINspISphIBglIIBspDIBGH polyadenylation sequenceBGH pA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctagtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatNaeINgoMIV4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgattagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctBarIKflIMscIXbaIBsaAIFRTHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggcccttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggBsmBIEsp3IHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    gcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagcccgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggNcoIPvuIAsiSIEcoRIDraIIIHygromycin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    agacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggatctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctNdeIRsrIIHygromycin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ctgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcaggacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtcAjuIDraIIIHygromycin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    acgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacSacIIHygromycin5,6205,6405,6605,6805,7005,7205,7405,760
    tcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttaBsaAIHygromycin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttBsmINaeIPfoIPsiINgoMIVSV40 pA5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ctcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcagagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtBstZ17ISV40 pA6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagcgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgSapIBspQI6,3206,3406,3606,3806,4006,4206,4406,460
    tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccNspIPciIpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcpUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaaAlwNIpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctpUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    tctcaagaagatcctttgatcttttctacgggagagttcttctaggaaactagaaaagatgcccpUC ori7,1807,200
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7207 bp500055006000650050010001500SFFVG->A from pSLQ2349145-1005EKo271.2 (DNA)XZp031.2 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)oLSM-pUC_ori_R (DNA)oLSM-pUC_ori_F (DNA)pUC_oriR_Fv2 (DNA)EKo152.2 (DNA),AhdI,BsaIBglI,FspI,AclIAmp (bla),PvuIAclIXmnISspIbla promoterFlpInF1 (DNA),SgrDI,BglII,AlwNIFlpInF (DNA),EKo285.1 (DNA),MfeImissing in pSLQ2349,NheI,BmtImissing in pSLQ2349,A->T from pSLQ2349EcoRV,TspMI,XmaI,SmaI,BstEIIBbvCI,Bpu10I,AflIIBssHIIEK_SFFV_F2 (DNA),Eco53kI,SacI,Eco53kI,SacI,AscI,+4oAPL0133 (DNA)HygromycinDraIIIAjuISacIIHyg_F (DNA)BsaAI,PfoI,NgoMIV,NaeISV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiISV40 pA,BsmI,BstZ17IM13R Genewiz (DNA)M13-48REV Genewiz (DNA)pUCori5F (DNA),BspQI,SapIpUC_oriR_Rv2 (DNA),pUCoriR (DNA),PciI,NspIpUC oriAlwNI

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    7207

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