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pAAV-nEF-3x-EYFP-WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaaggatctgcgatcgctccgggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcctagacgctagcgaggcEagIMluINotIPvuISbfIAsiSIAflIIIL-ITRnEF promoter20406080100120140160
    gtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtcacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttgcccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcaMfeIEcoNInEF promoter180200220240260280300320340
    agtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgcctcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtcgacttcgaagctccccgagcgtagagaggaagtgcgcgggcggcgggatggactccggcggtaggtgcggccaactcagcgcaagacggcggagggcggacaccacggaggacttgacgcaggcggCspCIEcoNIBsu36IHindIIInEF promoter360380400420440460480500520
    gtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacagatccattcaaatttcgagtccagctctggcccggaaacaggccgcgagggaacctcggatggatctgagtcggccgagaggtgcgaaacggactgggacgaacgagttgagatgcagaaacaaagcaaaagacaagacgcggcaatgtctaggttcgacactggccgcggatKasINarISfoIPluTInEF promoter540560580600620640660680700
    cggatccataacttcgtataggatactttatacgaagttatgtagctggagcaatttgaaaagattgttgtattacctctagctgcgcttaataacttcgtatagtataccttatacgaagttatggcgcgccaaccccgacacttaccttgccggtggtgcagatgaacttcaggcctaggtattgaagcatatcctatgaaatatgcttcaatacatcgacctcgttaaacttttctaacaacataatggagatcgacgcgaattattgaagcatatcatatggaatatgcttcaataccgcgcggttggggctgtgaatggaacggccaccacgtctacttgaagtcAscIBamHIBstZ17Ilox2722loxNEYFP Exon 1CMV ...nor720740760780800820840860
    ggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcaccggtataacttcgtataaagtatcctatacgaagtccagtcgaacggcatccaccgtagcgggagcgggagcggcctgtgcgacttgaacaccggcaaatgcagcggcaggtcgagctggtcctacccgtggtggggccacttgtcgaggagcgggaacgagtggtaccaccgtggccatattgaagcatatttcataggatatgcttcaAgeINcoIEYFP Exon 1lox 2722 Rev8809009209409609801,0001,0201,040
    tatttgccttagttattcttagatagacccagaaattatcacttgtgcaaagaataacttcgtataaggtatactatacgaagttatgagctctgtgcccccccttttttttataaaattgtattaatgttatatacatatctcctgtatgtgacccatgtgcttatgactctatataaacggaatcaataagaatctatctgggtctttaatagtgaacacgtttcttattgaagcatattccatatgatatgcttcaatactcgagacacgggggggaaaaaaaatattttaacataattacaatatatgtatagaggacatacactgggtacacgaatactgagataPsiISacIXmnIBstZ17IEco53kICMV bplox N Rev1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taattaagaagttcctattcttcaaatagtataggaacttcgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttagaagttcctattcttcaaaaggtataggaacttcactagtcctcctgtactcaccttgaagaagatggtgcgctcctggacgtaattaattcttcaaggataagaagtttatcatatccttgaagcgtcttaccatcgacctaacatcgacgataatcgttatactttggagaatcttcaaggataagaagttttccatatccttgaagtgatcaggaggacatgagtggaacttcttctaccacgcgaggacctgcatPacISpeIF3F5EYFP Exon 2CE i...nor1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgggcgaagcactgcaggccgtagccgaaggtggtcacgagggtgggccagggcacgggcagctaaacacatgagaaagtcgacgaagttcctatactatttgaagaataggaacttcttcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgcccgcttcgtgacgtccggcatcggcttccaccagtgctcccacccggtcccgtgcccgtcgatttgtgtactctttcagctgcttcaaggatatgataaacttcttatccttgaagaaSalIHincIIEYFP Exon 2F3 RevCMV i...ptor1,4201,4401,4601,4801,5001,5201,5401,560
    gccttaacccagaaattatcactgttattctttagaatggtgcaaagagaagttcctataccttttgaagaataggaacttcgctagctggagagtggccagcccttctcatgttcagagaacatggttaactggttaagtcatgtctcaatttctgagaactgtcattctcggacggaattgggtctttaatagtgacaataagaaatcttaccacgtttctcttcaaggatatggaaaacttcttatccttgaagcgatcgacctctcaccggtcgggaagagtacaagtctcttgtaccaattgaccaattcagtacagagttaaagactcttgacagtaagagcctBmtIHpaINheIHincIICE bpF5 RevvLoxP1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aattgagtagctggttgaagattgctctagtattacagcaatttgaactgcgcttatcaatttctgagaagtgtctttctcggaaattgaggtaccttacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttcttaactcatcgaccaacttctaacgagatcataatgtcgttaaacttgacgcgaatagttaaagactcttcacagaaagagcctttaactccatggaatgaacatgtcgagcaggtacggctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagKpnIBsrGIAcc65IEYFP Exon 3vLox22721,7601,7801,8001,8201,8401,8601,8801,9001,920
    tcgttggggtctttgctcagggcggactggtagctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtagcaaccccagaaacgagtcccgcctgaccatcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctacccccacaagacgaccatcaccagccgctcgacgtgcgacggcaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacaEYFP Exon 31,9401,9601,9802,0002,0202,0402,0602,0802,100
    cggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtcctgtgggatctagaggccggtactatatctgcaacaccgacaacatcaacatgaggtcgaacacggggtcctacaacggcaggaggaacttcagctacgggaagtcgagctacgccaagtggtcccacagcgggagcttgaagtggagccgcgcccagaacatcaacggcagcaggacaccctagatctcXbaIEYFP Exon 32,1202,1402,1602,1802,2002,2202,2402,260
    tcaagattgctccagcaatcttgtaatactcaatttccgagaatgacagttctcagaaattgataagcagacaatcttgtaatacatgcagctagtcaagattgctccagctatcaatttccgagaaagacacttctcagaaattgagaattcgatatcaagcttatcgataatcagttctaacgaggtcgttagaacattatgagttaaaggctcttactgtcaagagtctttaactattcgtctgttagaacattatgtacgtcgatcagttctaacgaggtcgatagttaaaggctctttctgtgaagagtctttaactcttaagctatagttcgaatagctattagClaIBspDIEcoRIEcoRVHindIIIvLoxP RvLox2272 RevWPRE2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaaWPRE2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgaPflMIWPRE2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcgccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagZraIAatIISacIIWPRE2,8202,8402,8602,8802,9002,9202,9402,960
    ttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattatAfeIBbsIBplIBplIClaIEarITliIXhoIBglIIBspDIPaeR7IhGH polyAWPRE2,9803,0003,0203,0403,0603,0803,1003,1203,140
    aaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgccttttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaBbsICspCIEcoO109IhGH polyA3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaaBlpINsiISphIBbvCIhGH polyA3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctcgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaAloIEagINotIPmlIBsaAIBseYIBstXIRsrIIBstEIIR-ITRhGH polyA3,5203,5403,5603,5803,6003,6203,6403,660
    ttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgKasINarISbfISfoIPluTIR-ITRf1 Origin3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaaf1 Origin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgaAloIPsiIBsaAIDraIIIf1 Origin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgtt4,2204,2404,2604,2804,3004,3204,3404,360
    gctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaaZraIAatIIEcoO109I4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtcaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccaEarIAmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagctttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcXmnIAmpR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    caactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgPvuIScaIAmpR4,9204,9404,9604,9805,0005,0205,0405,060
    taaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgtFspIAmpR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    attaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacgtaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcAmpR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactAmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    gaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcacttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagtAlwNIpUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    agacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccBseYIpUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ctggccttttgctcacatgtgaccggaaaacgagtgtacaPciIAflIIIpUC ori6,320
    • Plasmid
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    • Linear Map
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    6320 bp450050005500600050010001500F3 RevSTARTL-ITR,SbfI,EagI,NotI,AflIII,MluI,AsiSI,PvuI,MfeI,EcoNInEF promoter,HindIII,EcoNI,Bsu36I,CspCIlox2722,KasI,NarI,SfoI,PluTI,BamHIloxN,CMV intron donor,EYFP Exon 1,BstZ17I,AscIkozak,lox 2722 Rev,NcoI,AgeIlox N Rev,CMV bp,XmnI,BstZ17I,Eco53kI,SacI,PsiIF3,F5,PacICE intron donor,EYFP Exon 2,SpeICMV intron acceptorSalI,HincIIXmnIScaIAmpR,PvuIFspIAlwNIpUC ori,BseYI,AflIII,PciI

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    6320