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JPZF1 CMV-TO-SCN1A-377(G2E, A104K, S109I)-deImmLink-NZF
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMV20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcCMV180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaNcoISnaBICMV360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgTATGAATGCAAGAAATGTGGGCGGCGTTTCGGCCGGCTTTCCAATTGTACACGTCACGAGAAAACCCACAGCGCTTGTACGGGAGAGCGCCCGCATGGCTGTCACTTGTGTGGttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacATACTTACGTTCTTTACACCCGCCGCAAAGCCGGCCGAAAGGTTAACATGTGCAGTGCTCTTTTGGGTGTCGCGAACATGCCCTCTCGCGGGCGTACCGACAGTGAACACACCAfeIAleIBbsIMfeIPmeIAflIIBmgBIBsrGIHindIIIMY2EC4KK6CG8RR10FG12RL14SN16CT18RH20EK22TH24SA26CT28GE30RP32HG34CH36LC38GA104Kjpzf1720740760780800820840860
    TAAAGCCTTTACACACTGCTCAGATTTGAGAAAACACGAAAGGACACATACAGGTGAGCGGCCGTACTCATGTGGAATATGTGGGAAATCCTTTTCTGATAGCAGCGCAAAGAGACGGCACTGTATCCTGCACACGGGAGAACGGCCTTTCGTCTGTGGGGTCTGCAAAATGGGAATTTCGGAAATGTGTGACGAGTCTAAACTCTTTTGTGCTTTCCTGTGTATGTCCACTCGCCGGCATGAGTACACCTTATACACCCTTTAGGAAAAGACTATCGTCGCGTTTCTCTGCCGTGACATAGGACGTGTGCCCTCTTGCCGGAAAGCAGACACCCCAGACGTTTTACCCTBsgIK40AF42TH44CS46DL48RK50HE52RT54HT56GE58RP60YS62CG64IC66GK68SF70SD72SS74AK76RR78HC80IL82HT84GE86RP88FV90CG92VC94KM96GA104Kjpzf18809009209409609801,0001,0201,040
    TTTTCTCTGCTTACGAGCTTAAAGCAGCATCACTCCATACATACCGGGGAACGACCATATGAGTGCAAAGAGTGTGGAAAGACCTTTAGGCAATGTAGTCACCTGAAAAGGCATCAAAGGATACATACTGGCGAGCGGCCCTACCAATGTGGGCAATGTGGTAAGAGCTTCAGACAAAAGAGACGAATGCTCGAATTTCGTCGTAGTGAGGTATGTATGGCCCCTTGCTGGTATACTCACGTTTCTCACACCTTTCTGGAAATCCGTTACATCAGTGGACTTTTCCGTAGTTTCCTATGTATGACCGCTCGCCGGGATGGTTACACCCGTTACACCATTCTCGAAGTCTGF98SL100LT102SL104KQ106HH108SI110HT112GE114RP116YE118CK120EC122GK124TF126RQ128CS130HL132KR134HQ136RI138HT140GE142RP144YQ146CG148QC150GK152SF154RA104Kjpzf11,0601,0801,1001,1201,1401,1601,1801,2001,220
    AATCTTCCAATCTGCATCAGCATCACCGCCTGCATCACACCGGTGAACGCCCTTATGAATGTAAGGAATGCGGAAAGACGTTCAGACAATGTTCACATCTCAAGCGGCATCAACGCATCCATACGGGAGAGAGGCCGTTCGAGTGTGCCGTCTGCGGAGAATCTTTTGTCAATCCTTAGAAGGTTAGACGTAGTCGTAGTGGCGGACGTAGTGTGGCCACTTGCGGGAATACTTACATTCCTTACGCCTTTCTGCAAGTCTGTTACAAGTGTAGAGTTCGCCGTAGTTGCGTAGGTATGCCCTCTCTCCGGCAAGCTCACACGGCAGACGCCTCTTAGAAAACAGTTAGGAgeIAleISgrAIQ156SS158NL160HQ162HH164RL166HH168TG170ER172PY174EC176KE178CG180KT182FR184QC186SH188LK190RH192QR194IH196TG198ER200PF202EC204AV206CG208ES210FV212NP214A104Kjpzf11,2401,2601,2801,3001,3201,3401,3601,3801,400
    GGCCGAACTCGCAGACCACGTTACCGTGCATgctgctATGggcATGagcggaggctctgAcggatctGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCCGGCTTGAGCGTCTGGTGCAATGGCACGTAcgacgaTACccgTACtcgcctccgagacTgcctagaCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCAvaIBlpIBsgIPasISphIBsoBIBstXIAE216LA218DH220VT222VH224A104KEG2QS4DE6RA8LL10DQ12LH14TL16LS18NT20DA22TG24LE26EI28DR30AL32GI34PE361468-1926A1A2M3G4M5S6G7G8S9D10G11S121432-1467linkerjpzf11,4201,4401,4601,4801,5001,5201,5401,560
    CTGGTGAACCAGGGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGGACCACTTGGTCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCTAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCPasIBmgBIEcoNIEcoRIPflMIPpuMILV38NQ40GQ42AL44EP46KQ48DG50GW52GS54MA56EE58FV60TL62KD64VG66MD68FT70LG72DW74EQ76LG78LE80QG82DT84FW86DT88AL90DN92CQ941468-19261,5801,6001,6201,6401,6601,6801,7001,7201,740
    ACCTGTTCCTGCTGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGACACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCtaTGGACAAGGACGACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGatPciIBstXIDL96FL98LD100PP102GG104GD106GL108GL110GH112EK114FP116SD118LD120LD122MF124NG126SL128EC130DM132ES134II136RS138EL140MD142AD144GL146DF148NF150DS152*1468-19261,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggccgctcgaggagGGCCCgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaccggcgagctcctcCCGGGcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttApaIAvaIBclIPmeITliIXhoIBsoBIPspXIPaeR7IPspOMIBGH polyadenylation sequencec-myc epitopePolyhi...e tag1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaaBbsIClaISphIBspDIBGH polyadenylation sequence2,1202,1402,1602,1802,2002,2202,2402,260
    ctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccgacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggBGH pA2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatcctXbaIFRT2,4602,4802,5002,5202,5402,5602,5802,6002,620
    acttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatgtgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctacBarIMscIBsaXIPflFIPshAITth111IHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagcccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggPstIBfuAIBspMIEcoRIDraIIIHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    ggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcNcoIPvuIAsiSIPflFIRsrIITth111IHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggtDraIIIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    acatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggctgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgAjuISacIIHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaScaIHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    ttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgttPfoIPsiISV40 pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagtatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcBstZ17ISV40 pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgaSapIBsaXIBspQI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccctgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggPciIpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    gcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgapUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccacpUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagcaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcBspHIpUC ori4,9204,9404,9604,9805,0005,0205,0405,060
    ttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgtAhdIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    atgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgtactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacAclIBsaIFspIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgaPvuIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    catggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttBcgIScaIAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    gtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaacacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttAclIXmnIAmp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    taagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagSspIBspHISgrDIbla promoterAmp (bla)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttaccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatMfeIBpu10I6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gggttaggcgttttgcgctgcttcgtacgggccagatatacgccccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMluIBsiWI6,3206,340
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    6343 bp450050005500600050010001500linkermut6 H166IA104K1432-14671468-1926

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    6343