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pSBtetTA-YP_NotISpeI
  • Sequence Map
    Editing disabled on read-only sequence.
    acctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgcccAhdIbla CDS20406080100120140160
    agggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagtcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcAseIBsrDIbla CDS180200220240260280300320340
    ttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagAclIFspIPvuIBsrDIbla CDS360380400420440460480500520
    agaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggatatcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctatScaIbla CDS540560580600620640660680700
    ataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaactatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgAclIXmnIApaLIbla CDS720740760780800820840860
    aggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccctccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaagggSspIBciVIBspHIbla promoterbla CDS8809009209409609801,0001,0201,040
    cgaaaagtgccacctgatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggaaattgtaagcgttaatattttgttaaaattggatccctatacagttgaagtcggaagtttacatacacttaagttggagtcattaaaactcgtttttcaactactccgcttttcacggtggactacgccacactttatggcgtgtctacgcattcctcttttatggcgtagtcctttaacattcgcaattataaaacaattttaacctagggatatgtcaacttcagccttcaaatgtatgtgaattcaacctcagtaattttgagcaaaaagttgatgaggSspIAflIIBamHI5'-ITR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    acaaatttcttgttaacaaacaatagttttggcaagtcagttaggacatctactttgtgcatgacacaagtcatttttccaacaattgtttacagacagattatttcacttataattcactgtatcacaattccagtgggtcagaagtttacatacactaagttcgactcctctgtgtttaaagaacaattgtttgttatcaaaaccgttcagtcaatcctgtagatgaaacacgtactgtgttcagtaaaaaggttgttaacaaatgtctgtctaataaagtgaatattaagtgacatagtgttaaggtcacccagtcttcaaatgtatgtgattcaagctgaggagacHpaIPsiICspCIPshAIHincII5'-ITR5'...R1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cagaatgcggcgatgtttcggtaaggggtccgctatctagacgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagaggtcttacgccgctacaaagccattccccaggcgatagatctgctcaaatgagggatagtcactatctcttgcatacagctcaaatgagggatagtcactatctcttgctacagctcaaatgagggatagtcactatctcttgcatacagctcaaatgagggatagtcactatctcTet-responsive promoter5'-proxITRTet operatorTet operatorTet operatorTet operator1,4201,4401,4601,4801,5001,5201,5401,560
    aacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgattaggcgtgtacggtgggcgcctataaaagcagagctcgtttagtgaaccgtcagattgcatacagctcaaatgagggatagtcactatctcttgcatacagctcaaatagggatagtcactatctcttgcatacagctcaaatgagggatagtcactatctcttgcatacagctaatccgcacatgccacccgcggatattttcgtctcgagcaaatcacttggcagtctSacIEco53kITet-responsive promoterTet operator1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcgcctggagccaattccaactcttttgccttatacctactttccgtaccacttcctaccctcgaaaggcctctgaggccAATTCGCGGCCGCatctagagagACTAGTaGTGGCCGCTGCAggcctgtcaggccaagcttccatcgatagacatgataagatacattgatgagtagcggacctcggttaaggttgagaaaacggaatatggatgaaaggcatggtgaaggatgggagctttccggagactccggTTAAGCGCCGGCGtagatctctcTGATCAtCACCGGCGACGTccggacagtccggttcgaaggtagctatctgtactattctatgtaactactcaClaINotISfiISfiISpeIBspDIHindIIITet-responsive promoterSV40-polyANotI SpeI drop-in1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttggacaaaccacaacaagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggagatgtgggaggttttttaaagcaagtaaacctgtttggtgttgttcttacgtcactttttttacgaaataaacactttaaacactacgataacgaaataaacattggtaatattcgacgttatttgttcaattgttgttgttaacgtaagtaaaatacaaagtccaagtccccctctacaccctccaaaaaatttcgttcatHpaIPsiIHincIISV40-polyA1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aaacctctacaaatgtggtacctaggcgagaccctgtctcacaaaataaagtaagcccggactgagtgcggaaaggcgggcctggcgggtctggtctccccatgcgggccaccagaggccctgcagccttcagtcgcttgaaggggtaatggcgcttccactcacaaacatggcgtttggagatgtttacaccatggatccgctctgggacagagtgttttatttcattcgggcctgactcacgcctttccgcccggaccgcccagaccagaggggtacgcccggtggtctccgggacgtcggaagtcagcgaacttccccattaccgcgaaggtgagtgtttgtaccgcKpnIAvrIIPflFIAcc65ITth111IRPL13a promoterRPBSA constitutive promoterSV40-polyA2,1202,1402,1602,1802,2002,2202,2402,260
    gacagagcgtgtgaacgagatgaacagcccctcaaaaatatggccgccgaggctggacggccgtgccccagcagcaccgcctccgcgccccacgtgatctctcgccgggcacagcgctgaccgcggaggtccaaccggaagaatgtccggattggacattcggaagagggcccgcctgtctcgcacacttgctctacttgtcggggagtttttataccggcggctccgacctgccggcacggggtcgtcgtggcggaggcgcggggtgcactagagagcggcccgtgtcgcgactggcgcctccaggttggccttcttacaggcctaacctgtaagccttctcccgggcgAfeIApaIPmlIBsaAIBspEISacIIPspOMIRPL13a promoterRPBSA constitutive promoter2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cttccctggggaatctctgcgcacgcgcagaacgcttcgaccaatgaaaacacaggaagccgtccgcgcaaccgcgttgcgtcacttctgccgcccctgtttcaaggtatatagccgtagacggaacttcgcctttctctcggccttagcgccatttttttgggtgagtgtttttgaagggaccccttagagacgcgtgcgcgtcttgcgaagctggttacttttgtgtccttcggcaggcgcgttggcgcaacgcagtgaagacggcggggacaaagttccatatatcggcatctgccttgaagcggaaagagagccggaatcgcggtaaaaaaacccactcacaaaaaAccIFspIBstXIRPBSA constitutive promoterRPL13a promoterRPL41 exon1RPL...n12,4602,4802,5002,5202,5402,5602,5802,6002,620
    tggttcctgcgttgggattccgtgtacaatccatagacatctgacctcggcacttagcatcatcacagcaaactaactgtagcctttctctctttccctgtagaaacctctgcacctgaggccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggaccaaggacgcaaccctaaggcacatgttaggtatctgtagactggagccgtgaatcgtagtagtgtcgtttgattgacatcggaaagagagaaagggacatctttggagacgtggactccggtggtaccactcgttcccgctcctcgacaagtggccccaccacgggtaggaccNcoIBsrGIBsu36IMV2SK4GE6EL8FT10GV12VP14IL162752-3468RPBSA constitutive promoterRPL41 intron1YFP VenusRP...22,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagctgatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgggctacggcctccagtgcttcgcccgctaagctcgacctgccgctgcatttgccggtgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcgactagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggacccgatgccggaggtcacgaagcgggcgatVE18LD20GD22VN24GH26KF28SV30SG32EG34EG36DA38TY40GK42LT44LK46LI48CT50TG52KL54PV56PW58PT60LV62TT64LG66YG68LQ70CF72AR74Y2752-3468YFP Venus2,8202,8402,8602,8802,9002,9202,9402,960
    ccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggagggggctggtgtacttcgtcgtgctgaagaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagctgaagttcctcPfoIP76DH78MK80QH82DF84FK86SA88MP90EG92YV94QE96RT98IF100FK102DD104GN106YK108TR110AE112VK114FE116GD118TL120VN122RI124EL126KG128ID130FK132E2752-3468YFP Venus2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacgctgccgttgtaggaccccgtgttcgacctcatgttgatgttgtcggtgttgcagatatagtggcggctgttcgtcttcttgccgtagttccggttgaagttctaggcggtgttgtagctcctgccgccgcacgtcgagcggctggtgatggtcgtcttgtgggggtagccgctgcD134GN136IL138GH140KL142EY144NY146NS148HN150VY152IT154AD156KQ158KN160GI162KA164NF166KI168RH170NI172ED174GG176VQ178LA180DH182YQ184QN186TP188IG190D2752-3468YFP Venus3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggaAttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagggcagtggagctactaacttcagcctgctgaacggggcacgacgacgggctgttggtgatggactcgatggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctTaagcactggcggcggccctagtgagagccgtacctgctcgacatgttcccgtcacctcgatgattgaagtcggacgacttBsrGICspCIEcoRIG192PV194LL196PD198NH200YL202SY204QS206AL208SK210DP212NE214KR216DH218MV220LL222EF224VT226AA228GI230TL232GM234DE236LY238K2752-3468YFP VenusP2A3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gcaggctggtgacgtcgaggagaatcctggccccatgtctagactggacaagagcaaagtcataaacggcgctctggaattactcaatggagtcggtatcgaaggcctgacgacaaggaaactcgctcaaaagctgggagttgagcagcctaccctgtactggcacgtgaagaaccgtccgaccactgcagctcctcttaggaccggggtacagatctgacctgttctcgtttcagtatttgccgcgagaccttaatgagttacctcagccatagcttccggactgctgttcctttgagcgagttttcgaccctcaactcgtcggatgggacatgaccgtgcacttcttgPmlIXcmIBsaAIMS2RL4DK6SK8VI10NG12AL14EL16LN18GV20GI22EG24LT26TR28KL30AQ32KL34GV36EQ38PT40LY42WH44VK46NrtTArtTA-Advanced (improved tet-controlled transactivator)P2A3,5203,5403,5603,5803,6003,6203,6403,660
    aagcgggccctgctcgatgccctgccaatcgagatgctggacaggcatcatacccacttctgccccctggaaggcgagtcatggcaagactttctgcggaacaacgccaagtcattccgctgtgctctcctctcacatcgcgacggggctaaagtgcatctcggcacccgcccaattcgcccgggacgagctacgggacggttagctctacgacctgtccgtagtatgggtgaagacgggggaccttccgctcagtaccgttctgaaagacgccttgttgcggttcagtaaggcgacacgagaggagagtgtagcgctgccccgatttcacgtagagccgtgggcgggttApaINruIPspOMIK48RA50LL52DA54LP56IE58ML60DR62HH64TH66FC68PL70EG72ES74WQ76DF78LR80NN82AK84SF86RC88AL90LS92HR94DG96AK98VH100LG102TR104PrtTArtTA-Advanced (improved tet-controlled transactivator)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cagagaaacagtacgaaaccctggaaaatcagctcgcgttcctgtgtcagcaaggcttctccctggagaacgcactgtacgctctgtccgccgtgggccactttacactgggctgcgtattggaggaacaggagcatcaagtagcaaaagaggaaagagagacacctaccaccgagtctctttgtcatgctttgggaccttttagtcgagcgcaaggacacagtcgttccgaagagggacctcttgcgtgacatgcgagacaggcggcacccggtgaaatgtgacccgacgcataacctccttgtcctcgtagttcatcgttttctcctttctctctgtggatggtggctPsrIT106EK108QY110ET112LE114NQ116LA118FL120CQ122QG124FS126LE128NA130LY132AL134SA136VG138HF140TL142GC144VL146EE148QE150HQ152VA154KE156ER158ET160PT162TD164rtTArtTA-Advanced (improved tet-controlled transactivator)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttctatgcccccacttctgagacaagcaattgagctgttcgaccggcagggagccgaacctgccttccttttcggcctggaactaatcatatgtggcctggagaaacagctaaagtgcgaaagcggcgggccggccgacgcccttgacgattttgacttagacatgctcccagccaagatacgggggtgaagactctgttcgttaactcgacaagctggccgtccctcggcttggacggaaggaaaagccggaccttgattagtatacaccggacctctttgtcgatttcacgctttcgccgcccggccggctgcgggaactgctaaaactgaatctgtacgagggtcggAloIFseINaeINdeIBfuAIBspMINgoMIVSM166PP168LL170RQ172AI174EL176FD178RQ180GA182EP184AF186LF188GL190EL192II194CG196LE198KQ200LK202CE204SG206GP208AD210AL212DD214FD216LD218ML220PA222rtTArtTA-Advanced (improved tet-controlled transactivator)VP164,0404,0604,0804,1004,1204,1404,1604,1804,200
    gatgcccttgacgactttgaccttgatatgctgcctgctgacgctcttgacgattttgaccttgacatgctccccgggtccggcgctactaacttcagcctgctgaagcaggctggtgacgtcgaggagaatcctggtcccatgaccgagtacaagcccacggtgcgcctcgccactacgggaactgctgaaactggaactatacgacggacgactgcgagaactgctaaaactggaactgtacgaggggcccaggccgcgatgattgaagtcggacgacttcgtccgaccactgcagctcctcttaggaccagggtactggctcatgttcgggtgccacgcggagcggtAvaISmaIXmaIBsoBITspMIDA224LD226DF228DL230DM232LP234AD236AL238DD240FD242LD244ML246PG248rtTArtTA-Advanced (improved tet-controlled transactivator)P2APuroR (pac fro...lboniger CDS)VP16VP164,2204,2404,2604,2804,3004,3204,3404,360
    cccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacgggggcgctgctgcaggggtcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctaggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccacacccagcgcctgctgccBsiWIBspEIPflFIRsrIIBstEIITth111IPuroR (pac from Streptomyces alboniger CDS)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcgcggcgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgcaccaaggaccggtggcagMscIBsaXISacIIPuroR (pac from Streptomyces alboniger CDS)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaagccgcagagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggctgcagctccacgggcttcPfoIBsmBIEsp3IBssHIIPuroR (pac from Streptomyces alboniger CDS)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gaccgcgcacctggtgcatgacccgcaagcccggtgcctgattcgaaggcctgtcgtgaagcttggggatcaattctctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactctggcgcgtggaccacgtactgggcgttcgggccacggactaagcttccggacagcacttcgaacccctagttaagagatctcgagcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgaBclISacIBstBISexAIBstAPIDraIIIEco53kIHindIIIbGH-pAPuroR (pac from S...s alboniger CDS)4,9204,9404,9604,9805,0005,0205,0405,060
    cccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctgggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagataccgaagactccgcctttcttggtcgaBbsISphIPvuIIbGH-pA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gggcctactagctactcgggaccccttaccgaaacatcgccgcattctgcagaggagtcgagtgtatgtaaacttctgacccactgggaatgtgatgaaagaaataaaagctgaaatgaatcattctctctactattattctgatatttcacattcttaaaataaagtggtgatccccggatgatcgatgagccctggggaatggctttgtagcggcgtaagacgtctcctcagctcacatacatttgaagactgggtgacccttacactactttctttattttcgactttacttagtaagagagatgataataagactataaagtgtaagaattttatttcaccactagAvaIKflIBsoBIPpuMI3'-ITR3'-proxITR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ctaactgacctaagacagggaatttttactaggattaaatgtcaggaattgtgaaaaagtgagtttaaatgtatttggctaaggtgtatgtaaacttccgacttcaactgtatagggatccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtagattgactggattctgtcccttaaaaatgatcctaatttacagtccttaacactttttcactcaaatttacataaaccgattccacatacatttgaaggctgaagttgacatatccctaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatBamHIEcoNI3'-ITR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagDrdIPciIAflIIIori5,6205,6405,6605,6805,7005,7205,7405,760
    agaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtatctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatBciVIori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcAlwNIApaLIori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagBspHIori6,3206,3406,3606,3806,400
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6407 bp450050005500600050010001500Tet operatorbla CDSoripSB Puro seq r1 (DNA)AhdI,BsrDI,AseIFspI,AclI,BsrDI,PvuIScaIAclIXmnI,ApaLIbla promoter,SspI,BspHI,BciVISspIBamHI,AflII,HincII,HpaI5'-ITR,PshAI5'-proxITR,PsiI,CspCITet operator,Tet operator,pSBtet-GP f1 (DNA)Tet operator,Tet operatorTet-responsive promoterPfoISexAI,BstAPI,DraIII,BstBI,HindIIIEco53kI,SacI,BclIbGH-pABbsI,SphI,PvuII3'-proxITR,AvaI,BsoBI,KflI,PpuMI3'-ITR,EcoNIBamHIAflIII,PciIDrdI,BciVIApaLIAlwNI,BspHI

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    6407