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  • 0 feature results
XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export
  • Sequence Map
    Editing disabled on read-only sequence.
    tccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatSgrDI20406080100120140160
    caacaaggcaaggcttgaccgacaattgcatgaagaatcGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTgttgttccgttccgaactggctgttaacgtacttcttagCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAgeIMfeIMfeIEF1Alpha180200220240260280300320340
    TTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGGACCGACGTCEF1Alpha360380400420440460480500520
    TACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGStuIStuIAflIIEF1Alpha540560580600620640660680700
    TAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGApaIPciIBfuAIBspMIPspOMIEF1Alpha720740760780800820840860
    ACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCFseIPasISacIEco53kIEF1Alpha8809009209409609801,0001,0201,040
    CGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGcGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGAGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCgCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTKasINarISfoIBbvCIPluTIBpu10IEF1AlphaEF1Alpha1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGaagctACTAGTtcgcACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACttcgaTGATCAagcgSpeIEF1Alphahomolog1,2401,2601,2801,3001,3201,3401,3601,3801,400
    caccATGTTGAAGCCATCATTACCATTCACATCCCTCTTATTCCTGCAGCTGCCCCTGCTGGGAGTGGGGGCGGCCGTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTCCGGCCgtggTACAACTTCGGTAGTAATGGTAAGTGTAGGGAGAATAAGGACGTCGACGGGGACGACCCTCACCCCCGCCGGCAAATGGGTATGCTACAAGGACTGATACGCCCGATAGGGATACTGCAGGGCCTGATACGTCCTAGGATAGGTATACTGCAAGGTCTAATGCGAGGCCGGNdeIBamHIML2KP4SL6PF8TS10LL12FL14QL16PL18LG20VG22AA24VY26PY28DV30PD32YA34GY36PY38DV40PD42YA44GS46YP48YD50VP52DY54AP56A1405-23673xHAhIL-2Rgamma Signal Peptidehomolog1,4201,4401,4601,4801,5001,5201,5401,560
    GCCGGTACCCTGAACACGACAATTCTGACGCCCAATGGGAATGAAGACACCACAGCTGATTTCTTCCTGACCACTATGCCCACTGACTCCCTCAGTGTTTCCACTCTGCCCCTCCCAGAGGTTCAGTGTTTTGTGTTCAATGTCGAGTACATGAATTGCACTTGGAACAGCAGCTCGGCCATGGGACTTGTGCTGTTAAGACTGCGGGTTACCCTTACTTCTGTGGTGTCGACTAAAGAAGGACTGGTGATACGGGTGACTGAGGGAGTCACAAAGGTGAGACGGGGAGGGTCTCCAAGTCACAAAACACAAGTTACAGCTCATGTACTTAACGTGAACCTTGTCGTCGABbsIKpnIAcc65IA58GT60LN62TT64IL66TP68NG70NE72DT74TA76DF78FL80TT82MP84TD86SL88SV90ST92LP94LP96EV98QC100FV102FN104VE106YM108NC110TW112NS114S1405-2367hIL-2Rgamma Ectodomainhomoolog1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CTGAGCCCCAGCCTACCAACCTCACTCTGCATTATTGGTACAAGAACTCGGATAATGATAAAGTCCAGAAGTGCAGCCACTATCTATTCTCTGAAGAAATCACTTCTGGCTGTCAGTTGCAAAAAAAGGAGATCCACCTCTACCAAACATTTGTTGTTCAGCTCCAGGACCCACGGACTCGGGGTCGGATGGTTGGAGTGAGACGTAATAACCATGTTCTTGAGCCTATTACTATTTCAGGTCTTCACGTCGGTGATAGATAAGAGACTTCTTTAGTGAAGACCGACAGTCAACGTTTTTTTCCTCTAGGTGGAGATGGTTTGTAAACAACAAGTCGAGGTCCTGGGTGCS116EP118QP120TN122LT124LH126YW128YK130NS132DN134DK136VQ138KC140SH142YL144FS146EE148IT150SG152CQ154LQ156KK158EI160HL162YQ164TF166VV168QL170QD172PR1741405-2367hIL-2Rgamma Ectodomain1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GGAACCCAGGAGACAGGCCACACAGATGCTAAAACTGCAGAATCTGGTGATCCCCTGGGCTCCAGAGAACCTAACACTTCACAAACTGAGTGAATCCCAGCTAGAACTGAACTGGAACAACAGATTCTTGAACCACTGTTTGGAGCACTTGGTGCAGTACCGGACTGACTGGGACCCTTGGGTCCTCTGTCCGGTGTGTCTACGATTTTGACGTCTTAGACCACTAGGGGACCCGAGGTCTCTTGGATTGTGAAGTGTTTGACTCACTTAGGGTCGATCTTGACTTGACCTTGTTGTCTAAGAACTTGGTGACAAACCTCGTGAACCACGTCATGGCCTGACTGACCCTGAjuIPasIPflMIEP176RR178QA180TQ182ML184KL186QN188LV190IP192WA194PE196NL198TL200HK202LS204ES206QL208EL210NW212NN214RF216LN218HC220LE222HL224VQ226YR228TD230WD2321405-2367hIL-2Rgamma Ectodomain1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CACAGCTGGACTGAACAATCAGTGGATTATAGACATAAGTTCTCCTTGCCTAGTGTGGATGGGCAGAAACGCTACACGTTTCGTGTTCGGAGCCGCTTTAACCCACTCTGTGGAAGTGCTCAGCATTGGAGTGAATGGAGCCACCCAATCCACTGGGGGAGCAATACTTCAAAAGGTGTCGACCTGACTTGTTAGTCACCTAATATCTGTATTCAAGAGGAACGGATCACACCTACCCGTCTTTGCGATGTGCAAAGCACAAGCCTCGGCGAAATTGGGTGAGACACCTTCACGAGTCGTAACCTCACTTACCTCGGTGGGTTAGGTGACCCCCTCGTTATGAAGTTTTCBlpIBstXIPflMIHS234WT236EQ238SV240DY242RH244KF246SL248PS250VD252GQ254KR256YT258FR260VR262SR264FN266PL268CG270SA272QH274WS276EW278SH280PI282HW284GS286NT288SK2901405-2367hIL-2Rgamma Ectodomain2,1202,1402,1602,1802,2002,2202,2402,260
    AGAATCCTTTCCTGTTTGCATTGGAAGCCGTGGTTATCTCTGTTGGCTCCATGGGATTGATTATCAGCCTTCTCTGTGTGTATTTCTGGCTGGGTACCgcggccgccaagagcaggatcaccagcgagggcgagtacatccccctggaccagatcgacatcaacgtgATGGCGAGTCTTAGGAAAGGACAAACGTAACCTTCGGCACCAATAGAGACAACCGAGGTACCCTAACTAATAGTCGGAAGAGACACACATAAAGACCGACCCATGGcgccggcggttctcgtcctagtggtcgctcccgctcatgtagggggacctggtctagctgtagttgcacTACCGCTCKpnINotIBsaBIAcc65IG1T2EN292PF294LF296AL298EA300VV302IS304VG306SM308GL310II312SL314LC316VY318FW320L1405-2367KS2RI4TS6EG8EY10IP12LD14QI16DI18NV20MA22S2383-3192A1A2A3hIL-2Rgamma TransmembraneTraffic signalhIL-2Rgamma EctodomainPCRhomologMCP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCAAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGTTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATN24FT26QF28VL30VD32NG34GT36GD38VT40VA42PS44NF46AN48GI50AE52WI54SS56NS58RS60QA62YK64VT66CS68VR70QS72SA74QN76RK78YT80I2383-3192MCP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    AAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCXcmIAsiSIBsaBIBstEIIK82VE84VP86KG88AW90RS92YL94NM96EL98TI100PI102FA104TN106SD108CE110LI112VK114AM116QG118LL120KD122GN124PI126PS128AI130AA132NS134GI136YG138G2383-3192MCP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCAgeIBplIBplIG140TG142GG144SM146AS148NF150TQ152FV154LV156DN158GG160TG162DV164TV166AP168SN170FA172NG174IA176EW178IS180SN182SR184SQ186AY188KV190TC192SV194RQ196SS1982383-3192MCP2,8202,8402,8602,8802,9002,9202,9402,960
    TGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGABstXIBstZ17IAQ200NR202KY204TI206KV208EV210PK212GA214WR216SY218LN220ME222LT224IP226IF228AT230NS232DC234EL236IV238KA240MQ242GL244LK246DG248NP250IP252SA254IA2562383-3192MCPhomolog2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GCGAATAGCGGAATTTACttctgctacgagaacgaggtgtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatCGCTTATCGCCTTAAATGaagacgatgctcttgctccacactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtaApaIBclIPmeITliIXhoIBseRIPspXIPaeR7IPspOMIAN258SG260IY262FC264YE266NE268V*2702383-3192c-myc epitopeBGH polyaden...on sequenceER exportMCPPolyhi...e taghomolog3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtBbsIBGH polyadenylation sequence3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacaacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtClaISphIBspDIBGH pA3,5203,5403,5603,5803,6003,6203,6403,660
    cttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttKflI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggacaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctBarIMscIXbaIPflFIPshAITth111IHygromycinFRT3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgccccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgEcoRIHygromycin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacaNdeIAsiSIBfuAIBspMIRsrIIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    gtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggcatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgaccPflFITth111IHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    agcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagAjuIHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcScaIHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttatgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaataPsiIHygromycinSV40 pA4,9204,9404,9604,9805,0005,0205,0405,060
    aatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattttaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaaBsmIBstZ17ISV40 pA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctcc5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttgPciISapIBspQIpUC ori5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    gatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccapUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtttctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccapUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    agctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagttBspHIpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgAhdI*W286HK284IL282SA280GI278EA276IQ274RN272RE270DM268TA266QS264GT262TY260IV2586386-7246Amp (bla)6,3206,3406,3606,3806,4006,4206,4406,460
    tacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgaBsaIBsrDIVI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA2006386-7246Amp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    agagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctctctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggagFspIBsrDILT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170EN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142G6386-7246Amp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcgctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagBcgIScaII140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84D6386-7246Amp (bla)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26P6386-7246Amp (bla)7,0207,0407,0607,0807,1007,1207,1407,160
    tgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggtactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaSspIBspHIH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M6386-7246Amp (bla)bla promoter7,1807,2007,2207,2407,2607,2807,3007,3207,340
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7345 bp550060006500700050010001500homoolog1405-23676386-7246EKo152.4 (DNA)XZp358.2 (DNA)FlpInF1 (DNA),FlpInF (DNA),EKo273.1 (DNA),SgrDI,MfeI,MfeI,AgeIStuI,AflIIEF1Alpha,StuI,BfuAI,BspMIPspOMI,ApaI,PciIFseI,PasIEco53kI,SacI,BbvCI,Bpu10I,KasI,NarIEF1Alpha,SfoI,PluTIEF1a-seq-F (DNA),IGF1R-seq-F0 (DNA)homolog,Kozak,hIL-2Rgamma Signal Peptide,XZp374.1 (DNA),SpeIhomolog,3xHA,XZp374.2 (DNA),XZp374.3 (DNA),BamHI,NdeIXZp374.4 (DNA)Acc65IKpnI,XZp374.5 (DNA),KpnI,BbsIpUCori5F (DNA)PciIpUCoriR (DNA)pUC oriBspHIEKo152.2 (DNA),AhdI,BsaI,BsrDIFspI,BsrDIAmp (bla),ScaI,BcgIbla promoter,SspI,BspHI

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    7345

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