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pSB1C3_BBa_K4720021
  • Sequence Map
Editing disabled on read-only sequence.
ATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCGAGCTCCGAACCTAAGAGTGGTTATTTTTTGCGGGCCGCCGTTGGCTCGCAAGACTTGTTTAGGTCTACCTCAAGACTCCAGTAATGACCTAGATAGTTGTCCTCAGAvaITliIXhoIBsoBIPspXIPaeR7ITO terminator20406080100120140160
CAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGTTCGCTCGAGCTATAGTTTAATGCGGGGCGGGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGTACCTTCGGTAGTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGCGGAACGCATATTATAAACGGGTACCACTTTTGCCBsmIBtgINcoISacIScaISspITatIBanIIEcoRVEco53kIcmR180200220240260280300320340
GGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCCCCGCTTCTTCAACAGGTATAACCGGTGCAAATTTAGTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAGAGTTATTTGGGAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTTATATACACATCTTTGACGGCCTTTAGCAGPasIBcoDIBsmAIBsmBIEsp3IPflMIcmR360380400420440460480500520
GTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCCACCATAAGTGAGGTCTCGCTACTTTTGCAAAGTCAAACGAGTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGTGGCAGAAAGTAACGGTATGCTTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACTTATTTCCGGCCTATTTTGAACACGAclIBsmIBspEIBsrDIcmR540560580600620640660680700
TTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTATAGGTCGACTTGCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACAAGAAATGCTACGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAAAATCGAAGGAATCGAGGACcmR720740760780800820840860
AAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAATTTTAGAGCTATTGAGTTTTTTATGCGGGCCATCACTAGAATAAAGTAATACCACTTTCAACCTTGGAGAATGCACGGGCTAGTTGAGCTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGTCTTAAAGTCTATTTAvaITliIXhoIZraIAatIIBsaHIBsoBIPspXIPaeR7IVF28809009209409609801,0001,0201,040
AAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGttgacggctagctcagtcctaggtacagtgctaGCTACTAGAGAAAGAGGAGAAATACTAGATGTGGCGTGAAACGAAGCTGCTGTTAATCGATGATAACCTGGACCGTTCTCGTGATTTTTTTAGGAATCGAAAGCGATTCCTACTAAAGACCTTAAGCGCCGGCGAAGATCTCaactgccgatcgagtcaggatccatgtcacgatCGATGATCTCTTTCTCCTCTTTATGATCTACACCGCACTTTGCTTCGACGACAATTAGCTACTATTGGACCTGGCAAGAGCACTAABmtIBmtIClaIEagINheINheINotIXbaIAvrIIBspDIEcoRIFleQ_Codon_OptimizedBBa_J23100prefixRBS...011,0601,0801,1001,1201,1401,1601,1801,2001,220
TAGCGGTGATATTGAATTTCTTAGGAGAGGACCAATTAACTTGTAATAGTGAAGACTGGAGAGAAGTTGCTGCTGGGCTCTCTAATTCTCGTGAGGCTCTCTGTGTTTTACTGGGATCCGTTGAATCTAAAGGTGGCGCAGTTGAACTCCTCAAACAGCTCGCTTCCTGGGATGAATCGCCACTATAACTTAAAGAATCCTCTCCTGGTTAATTGAACATTATCACTTCTGACCTCTCTTCAACGACGACCCGAGAGATTAAGAGCACTCCGAGAGACACAAAATGACCCTAGGCAACTTAGATTTCCACCGCGTCAACTTGAGGAGTTTGTCGAGCGAAGGACCCTACTBmrIBamHIBanIIFleQ_Codon_Optimized1,2401,2601,2801,3001,3201,3401,3601,3801,400
ATATTTACCAATTTTGTTAATTGGTGAACCAGCGCCAGCTGATTGGCCTGAGGAATTGCGTCGACGAGTTCTTGCATCTTTGGAAATGCCGCCGTCCTATAATAAATTACTGGACTCTTTACACCGTGCTCAAGTTTATCGTGAAATGTATGATCAAGCACGTGAACGGGGACGCTATAAATGGTTAAAACAATTAACCACTTGGTCGCGGTCGACTAACCGGACTCCTTAACGCAGCTGCTCAAGAACGTAGAAACCTTTACGGCGGCAGGATATTATTTAATGACCTGAGAAATGTGGCACGAGTTCAAATAGCACTTTACATACTAGTTCGTGCACTTGCCCCTGCGBclISalISspIBstXIPflFISgrDIBsu36ITth111IFleQ_Codon_Optimized1,4201,4401,4601,4801,5001,5201,5401,560
AGTCGTGAACCAAATTTATTTAGATCGCTCGTAGGTACGAGTCGCGCCATACAACAAGTTCGACAAATGATGCAACAAGTAGCGGACACTGACGCTTCAGTTTTGATTCTGGGTGAAAGCGGGACAGGGAAAGAGGTAGTCGCCCGAAATTTACACTATCACAGCAAACGTCGTGTCAGCACTTGGTTTAAATAAATCTAGCGAGCATCCATGCTCAGCGCGGTATGTTGTTCAAGCTGTTTACTACGTTGTTCATCGCCTGTGACTGCGAAGTCAAAACTAAGACCCACTTTCGCCCTGTCCCTTTCTCCATCAGCGGGCTTTAAATGTGATAGTGTCGTTTGCAGCACFleQ_Codon_Optimized1,5801,6001,6201,6401,6601,6801,7001,7201,740
AAGGACCATTTGTTCCTGTTAATTGTGGTGCCATTCCAGCCGAGCTTTTAGAGTCGGAGCTGTTTGGTCATGAAAAGGGCGCATTTACAGGGGCGATTACTAGCCGAGCTGGGCGCTTTGAACTTGCTAATGGTGGTACGTTGTTTCTTGATGAAATAGGAGATATGCCCCTTCCTTCCTGGTAAACAAGGACAATTAACACCACGGTAAGGTCGGCTCGAAAATCTCAGCCTCGACAAACCAGTACTTTTCCCGCGTAAATGTCCCCGCTAATGATCGGCTCGACCCGCGAAACTTGAACGATTACCACCATGCAACAAAGAACTACTTTATCCTCTATACGGGGAAGGAloIBanINmeAIIINmeAIIIFleQ_Codon_Optimized1,7601,7801,8001,8201,8401,8601,8801,9001,920
CATGCAAGTAAAACTCTTACGTGTTTTACAAGAACGTACGTTTGAGCGCGTAGGTAGTAATAAAACCCAAAATGTAGATGTTCGTATAATAGCGGCTACGCATAAGAATCTGGAAAAGATGATAGAAGACGGGACCTTTCGTGAGGATTTATATTATCGTCTGAATGTGTTTCCTGTACGTTCATTTTGAGAATGCACAAAATGTTCTTGCATGCAAACTCGCGCATCCATCATTATTTTGGGTTTTACATCTACAAGCATATTATCGCCGATGCGTATTCTTAGACCTTTTCTACTATCTTCTGCCCTGGAAAGCACTCCTAAATATAATAGCAGACTTACACAAAGGABsiWIPpuMIEcoO109IFleQ_Codon_Optimized1,9401,9601,9802,0002,0202,0402,0602,0802,100
ATCGAAATGGCCCCTTTGCGTGAACGAGTGGAAGATATAGCATTATTACTTAATGAGCTTATTAGTCGTATGGAACACGAAAAGCGGGGAAGTATTCGTTTTAATAGCGCCGCTATTATGTCACTGTGTCGTCATGATTGGCCAGGAAATGTTAGAGAGCTTGCTAATCTCGTTGTAGCTTTACCGGGGAAACGCACTTGCTCACCTTCTATATCGTAATAATGAATTACTCGAATAATCAGCATACCTTGTGCTTTTCGCCCCTTCATAAGCAAAATTATCGCGGCGATAATACAGTGACACAGCAGTACTAACCGGTCCTTTACAATCTCTCGAACGATTAGAGCAACFleQ_Codon_Optimized2,1202,1402,1602,1802,2002,2202,2402,260
AAAGACTTGCAATAATGCACCCGTATGGCGTTATAGGTGTTGGGGAGTTACCTAAGAAGTTTCGACACGTGGATGATGAAGATGAACAGCTTGCATCTTCCTTAAGAGAGGAATTGGAAGAAAGAGCCGCAATTAATGCAGGCCTTCCCGGTATGGATGCTCCCGCTATGCTTCCTTTCTGAACGTTATTACGTGGGCATACCGCAATATCCACAACCCCTCAATGGATTCTTCAAAGCTGTGCACCTACTACTTCTACTTGTCGAACGTAGAAGGAATTCTCTCCTTAACCTTCTTTCTCGGCGTTAATTACGTCCGGAAGGGCCATACCTACGAGGGCGATACGAAGGAseIStuIAflIIBstAPIFleQ_Codon_Optimized2,2802,3002,3202,3402,3602,3802,4002,4202,440
CGCAGAGGGACTTGATTTAAAAGATTATTTGGCTAATTTAGAACAAGGGTTGATTCAACAAGCTCTGGATGATGCAGGCGGCGTTGTTGCCCGCGCAGCGGAGCGTCTTCGTATACGGCGGACTACATTAGTTGAAAAGATGCGTAAATATGGTATGAGTCGGCGTGACGACGACGCGTCTCCCTGAACTAAATTTTCTAATAAACCGATTAAATCTTGTTCCCAACTAAGTTGTTCGAGACCTACTACGTCCGCCGCAACAACGGGCGCGTCGCCTCGCAGAAGCATATGCCGCCTGATGTAATCAACTTTTCTACGCATTTATACCATACTCAGCCGCACTGCTGCTGBstZ17IFleQ_Codon_Optimized2,4602,4802,5002,5202,5402,5602,5802,6002,620
CTGTCGGATGATTGATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGAGTAACACCGTGCGTGTGACAGCCTACTAACTATGATCTCGGTCCGTAGTTTATTTTGCTTTCCGAGTCAGCTTTCTGACCCGGAAAGCAAAATAGACAACAAACAGCCACTTGCGAGAGATGATCTCAGTGTGACCGAGTGGAAGCCCACCCGGAAAGACGCAAATATATGATCTCATTGTGGCACGCACAAhdIBmrIPsrIdouble terminator FleQ...zedPcl ...0512,6402,6602,6802,7002,7202,7402,7602,7802,800
TGACTATTTTACCTCTGGCGGTGATAATGGTTGCTACTAGAGCGCCTAAAAATTGACAGTTTCCGCTTAAAAATTTAGCAATTAGCATATTTAGTCATTAGATTGACGTTAATCGCCTGCGGCTGCTTTGCCCGTGCCCGCACTGCCAGGGATTCGACGTTGCCACCGGAAACCGACTGATAAAATGGAGACCGCCACTATTACCAACGATGATCTCGCGGATTTTTAACTGTCAAAGGCGAATTTTTAAATCGTTAATCGTATAAATCAGTAATCTAACTGCAATTAGCGGACGCCGACGAAACGGGCACGGGCGTGACGGTCCCTAAGCTGCAACGGTGGCCTTTGGCBtsIBtsαIPpel K4242001Pcl lamR00512,8202,8402,8602,8802,9002,9202,9402,960
CATCCATGCTTCCGTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAAGTAGGTACGAAGGCATGATCTCTTTCTCCTCTTTATGATCTACGCATTTCCTCTTCTTGAAAAGTGACCTCAACAGGGTTAAGAACAACTTAATCTACCACTACAATTACCCGTGTTTAAAAGACAGTCACCTCTCCCACTTCCACTACGTTGTATGCCTTTTGAATGGGAATTTGFPPpel...001RBS...FP2,9803,0003,0203,0403,0603,0803,1003,1203,140
TTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGAAATAAACGTGATGACCTTTTGATGGACAAGGTACCGGTTGTGAACAGTGATGAAAGCCAATACCACAAGTTACGAAACGCTCTATGGGTCTAGTATACTTTGTCGTACTGAAAAAGTTCTCACGGTACGGGCTTCCAATACATGTCCTTTCTTGATATAAAAAGTTTCTACTGCBarIBtgINcoINdeITatIBsrGIGFP3,1603,1803,2003,2203,2403,2603,2803,3003,320
GGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAACCTTGATGTTCTGTGCACGACTTCAGTTCAAACTTCCACTATGGGAACAATTATCTTAGCTCAATTTTCCATAACTAAAATTTCTTCTACCTTTGTAAGAACCTGTGTTTAACCTTATGTTGATATTGAGTGTGTTACATATGTAGTACCGTCTGTTTGTTTTCTTACCTTAGTTBaeIBstZ17IGFP3,3403,3603,3803,4003,4203,4403,4603,4803,500
AGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTTCAATTGAAGTTTTAATCTGTGTTGTAACTTCTACCTTCGCAAGTTGATCGTCTGGTAATAGTTGTTTTATGAGGTTAACCGCTACCGGGACAGGAAAATGGTCTGTTGGTAATGGACAGGTGTGTTAGACGGGAAAGCTTTCTAGGGTTGCTTTTCTCTCTGGTGTACCAGGAABsaIDrdIHpaIMfeIBcoDIBsmAIBstBIGFP3,5203,5403,5603,5803,6003,6203,6403,660
CTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGaaaaaaaaaccccgcccctgacagggcggggttttttttTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAGAACTCAAACATTGTCGACGACCCTAATGTGTACCGTACCTACTTGATATGTTTATTATTATGATCTCtttttttttggggcggggactgtcccgccccaaaaaaaaATGATCATCGCCGGCGACGTCAGGCCGTTTTTTCCCGTTCCACAGTGGTGGGACGGGAAAAAGAAATTEagINotIPstISpeIAlwNIGFPterminatorTerminator_B1006suffix3,6803,7003,7203,7403,7603,7803,8003,8203,840
AACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGTTGGCTTTTCTAATGAAGCGCAATACGTCCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCNspIPciIBsrBIterminatorVR3,8603,8803,9003,9203,9403,9603,9804,0004,020
GAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTGTCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAADrdIBciVIori_pMB14,0404,0604,0804,1004,1204,1404,1604,1804,200
ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCTGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGApaLIori_pMB14,2204,2404,2604,2804,3004,3204,3404,360
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAAlwNIori_pMB14,3804,4004,4204,4404,4604,4804,5004,5204,540
TGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATori_pMB14,5604,5804,6004,6204,6404,6604,6804,7004,720
TCAAAAAGGATCTTCACCTAGAGTTTTTCCTAGAAGTGGATC4,740
  • Plasmid
Editing disabled on read-only sequence.
4746 bp350037504000425045002505007501000

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4746