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EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP
  • Sequence Map
    Editing disabled on read-only sequence.
    gagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatcccagagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtagggtctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcgaaaggaPasISrfIcore insul20406080100120140160
    ctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgacgattgagtGGCAGGCCCGCAGCGGGGAGGAGGaggagGAGCCGCCGAAGGAGCGAGCCTCTatcagtctagagggtatataatggatcccatcgcggacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaactgctaactcaCCGTCCGGGCGTCGCCCCTCCTCCtcctcCTCGGCGGCTTCCTCGCTCGGAGAtagtcagatctcccatatattacctagggtagcgcMfeISfiIStuIXbaIcore insulUTRN Prom...-43 SiteUTRN Prom...-14 Site180200220240260280300320340
    tctcagcctcactttgagctcctccacacgaattccccgataccgtcgattcaaggagcttgcttgttctttttgcagaagctcagaataaacgctcaactttggcagatctaccaagcttggtaccgagctcggatccaactcgaggccaccatgccagagccagcgaagtctgagagtcggagtgaaactcgaggaggtgtgcttaaggggctatggcagctaagttcctcgaacgaacaagaaaaacgtcttcgagtcttatttgcgagttgaaaccgtctagatggttcgaaccatggctcgagcctaggttgagctccggtggtacggtctcggtcgcttcagacKpnITliIXhoIBglIIPspXIAcc65IPaeR7IHindIIIMP2EP4AK6S504-893Xenopus globin 5'-UTRH2B360380400420440460480500520
    ctcccgccccgaaaaagggctccaagaaggcggtgactaaggcgcagaagaaaggcggcaagaagcgcaagcgcagccgcaaggagagctattccatctatgtgtacaaggttctgaagcaggtccaccctgacaccggcatttcgtccaaggccatgggcatcatgaattcgttgagggcggggctttttcccgaggttcttccgccactgattccgcgtcttctttccgccgttcttcgcgttcgcgtcggcgttcctctcgataaggtagatacacatgttccaagacttcgtccaggtgggactgtggccgtaaagcaggttccggtacccgtagtacttaagcaaBsrGIA8PA10PK12KG14SK16KA18VT20KA22QK24KG26GK28KR30KR32SR34KE36SY38SI40YV42YK44VL46KQ48VH50PD52TG54IS56SK58AM60GI62MN64SF66504-893H2B540560580600620640660680700
    tgtgaacgacattttcgagcgcatcgcaggtgaggcttcccgcctggcgcattacaacaagcgctcgaccatcacctccagggagatccagacggccgtgcgcctgctgctgcctggggagttggccaagcacgccgtgtccgagggtactaaggccatcaccaagtacaccagcacacttgctgtaaaagctcgcgtagcgtccactccgaagggcggaccgcgtaatgttgttcgcgagctggtagtggaggtccctctaggtctgccggcacgcggacgacgacggacccctcaaccggttcgtgcggcacaggctcccatgattccggtagtggttcatgtggtcgAfeIMscIPaqCIVN68DI70FE72RI74AG76EA78SR80LA82HY84NK86RS88TI90TS92RE94IQ96TA98VR100LL102LP104GE106LA108KH110AV112SE114GT116KA118IT120KY122TS124504-893H2B720740760780800820840860
    gctaaggatccccgggtaccggtcGTCTCAAAGGGCGAGGAACTTTTTACGGGCGTTGTTCCGATCCTCGTCGAATTGGACGGGGACGTTAATGGGCATAAATTCTCAGTTAGTGGTGAGGGTGAGGGGGACGCGACATATGGGAAACTTACGCTGAAGTTCATATGTACCACCGcgattcctaggggcccatggccagCAGAGTTTCCCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGGAGCAGCTTAACCTGCCCCTGCAATTACCCGTATTTAAGAGTCAATCACCACTCCCACTCCCCCTGCGCTGTATACCCTTTGAATGCGACTTCAAGTATACATGGTGGCAfeIAgeIKpnIAcc65IBpu10IAK126DP128RV130504-893PV2VS4KG6EE8LF10TG12VV14PI16LV18EL20DG22DV24NG26HK28FS30VS32GE34GE36GD38AT40YG42KL44TL46KF48IC50TT52894-1613GFPlinker8809009209409609801,0001,0201,040
    GAAAATTGCCaGTGCCGTGGCCTACTCTCGTCACCACCCTGACATATGGTGTTCAGTGTTTTAGCCGGTATCCAGACCATATGAAACAGCACGATTTTTTTAAATCCGCTATGCCTGAAGGTTACGTACAGGAGCGAACGATATTTTTTAAAGACGACGGAAACTACAAAACTAGCTTTTAACGGtCACGGCACCGGATGAGAGCAGTGGTGGGACTGTATACCACAAGTCACAAAATCGGCCATAGGTCTGGTATACTTTGTCGTGCTAAAAAAATTTAGGCGATACGGACTTCCAATGCATGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTTTGATGTTTTGATCBarISnaBIGK54LP56VP58WP60TL62VT64TL66TY68GV70QC72FS74RY76PD78HM80KQ82HD84FF86KS88AM90PE92GY94VQ96ER98TI100FF102KD104DG106NY108KT110R894-1613GFP1,0601,0801,1001,1201,1401,1601,1801,2001,220
    AGCCGAGGTAAAATTCGAGGGTGACACTCTCGTGAACCGGATCGAACTGAAGGGCATAGATTTTAAGGAGGATGGCAATATACTGGGCCACAAGCTCGAATACAACTATAATTCTCATAATGTCTATATCATGGCGGACAAACAGAAAAACGGCATCAAGGTAAACTTTAAAATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAGCACTTGGCCTAGCTTGACTTCCCGTATCTAAAATTCCTCCTACCGTTATATGACCCGGTGTTCGAGCTTATGTTGATATTAAGAGTATTACAGATATAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCATTTGAAATTTTAGAleIA112EV114KF116EG118DT120LV122NR124IE126LK128GI130DF132KE134DG136NI138LG140HK142LE144YN146YN148SH150NV152YI154MA156DK158QK160NG162IK164VN166FK168I894-1613GFP1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CGACACAATATTGAGGACGGCTCTGTCCAGCTTGCGGACCATTACCAACAGAACACGCCGATAGGGGATGGTCCAGTACTTCTCCCTGATAATCACTATTTGTCTACTCAGTCAGCTCTTAGCAAGGACCCAAACGAAAAACGGGATCACATGGTCCTTCTTGAGTTCGTCACTGGCTGTGTTATAACTCCTGCCGAGACAGGTCGAACGCCTGGTAATGGTTGTCTTGTGCGGCTATCCCCTACCAGGTCATGAAGAGGGACTATTAGTGATAAACAGATGAGTCAGTCGAGAATCGTTCCTGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAACTCAAGCAGTGACAccIPpuMIEcoO109IR170HN172IE174DG176SV178QL180AD182HY184QQ186NT188PI190GD192GP194VL196LP198DN200HY202LS204TQ206SA208LS210KD212PN214EK216RD218HM220VL222LE224FV226T894-1613GFP1,4201,4401,4601,4801,5001,5201,5401,560
    CGGCTGGGATAACACTCGGGATGGACGAACTGTATAAGtagctagcgaattcgaatttaaatcggcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaGCCGACCCTATTGTGAGCCCTACCTGCTTGACATATTCatcgatcgcttaagcttaaatttagccggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactccttBmtINheISwaIBstBI*1A228AG230IT232LG234MD236EL238YK240894-1613BGH polyadenylation sequenceGFPMCS1,5801,6001,6201,6401,6601,6801,7001,7201,740
    attgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggcttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaBbsINotIXmnIBGH polyadenylation sequence5, 299 FpSV401,7601,7801,8001,8201,8401,8601,8801,9001,920
    ccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgSexAIpSV401,9401,9601,9802,0002,0202,0402,0602,0802,100
    ccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgatgaaaggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcactactttBclIPmlIStuIAvrIIpSV402,1202,1402,1602,1802,2002,2202,2402,260
    aagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagZraIAatIIPflFIPshAITth111IHygro2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtaHygro2, 563 F2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctcctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgaAsiSIPflFITth111IHygro2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctHygro2,8202,8402,8602,8802,9002,9202,9402,960
    ggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgaccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctAjuISacIIHygro3, 135 F2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataggtcgacaatcaacctctggattacaaaattgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatccagctgttagttggagacctaatgttttaaAccISalIHygroWPRE6, 730 R3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcatacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaBsmIHpaIPsiISV40 pAWPRESV40 pA(1)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttcccaaactgggttcttatatcccttgctctagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaagggtttgacccaagaatatagggaacgagaBsaISV40 pASV40 pA(1)3,5203,5403,5603,5803,6003,6203,6403,660
    ggtcaaccaggttgcagggtttcctgtcctcacaggaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggctcaaagagcagcgagaagcgttcccagttggtccaacgtcccaaaggacaggagtgtccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtccgagtttctcgtcgctcttcgcaagApaIEcoNISexAIPspOMIEcoO109Icore insul(1) Geneious name: core insul(1)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    agaggaaagcgatcccgtgccaccttccccgtgcccgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggggctttgggggggggctgtccctctcctttcgctagggcacggtggaaggggcacgggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccccgaaacccccccccgacagggPasISrfIcore insul(1) Geneious name: core insul(1)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacactatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgEcoRV4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtctttttttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaBsmI3'TR4,2204,2404,2604,2804,3004,3204,3404,360
    tccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccataggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtaMscI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgcgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgacBsgI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgcctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacBssHIIpLac(F)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    taaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacSapIBspQIPvuII4,9204,9404,9604,9805,0005,0205,0405,060
    cgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctggcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggacPciIAflIII5,0805,1005,1205,1405,1605,1805,2005,2205,240
    acgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagtgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatc5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgAlwNI5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaac5,6205,6405,6605,6805,7005,7205,7405,760
    tttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttag5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcAhdIBsaIBsrDIAmp5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattggctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacAclIFspIBsrDIAmp6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataAmp6,3206,3406,3606,3806,4006,4206,4406,460
    ggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagBcgIAmp6,4806,5006,5206,5406,5606,5806,6006,6206,640
    attggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgataaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctAclIXmnIAmp6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttggtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacCspCIAmp6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttAloIPsiI7,0207,0407,0607,0807,1007,1207,1407,160
    ccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgccc7,1807,2007,2207,2407,2607,2807,3007,3207,340
    cgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgaFspIPvuII7,3607,3807,4007,4207,4407,4607,4807,5007,520
    gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgccgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgBssHIIBII(R)7,5407,5607,5807,6007,6207,6407,6607,6807,700
    agaacacgcagctagattaaccctagaaagataatcatattgtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattctcttgtgcgtcgatctaattgggatctttctattagtataacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagBsiWI31bp spacer5'TR(1)5'TR5'TR7,7207,7407,7607,7807,8007,8207,8407,860
    aacaaacaatttatttatgtttatttatttattaaaaaaaacaaaaactcaaaatttcttctataaagtaacaaaacttttatttgtttgttaaataaatacaaataaataaataattttttttgtttttgagttttaaagaagatatttcattgttttgaaaata7,8807,9007,9207,940
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7958 bp5500600065007000750050010001500pSV40GATCMluI-PGK-GF1614-1616504-893894-1613EWo174.4 (DNA)M13-48REV Genewiz (DNA)core insul,Added MluI site,Gibson overlap,UTRN Promoter -43 Site,+12Xenopus globin 5'-UTR,added SpeI site,Added XhoI site,Kozak,+10H2B,PaqCI,AfeI,MscIlinker,linker,EKo147.1 (DNA),GFP5 (DNA),NK-gfp-seq-r (DNA),AfeI,+4pNSK084 (DNA)EKo224.2 (DNA),BarI,SnaBIAleIGFP,pPYK028 (DNA)GFP6 (DNA),AccI,EcoO109I,PpuMIMCS,BGHR Genewiz (DNA),NheI,BmtI,BstBI,SwaIBGH polyadenylation sequence,BghR1 (DNA)NotI,5, 299 F,bghF (DNA),BbsI,NotI,XmnIES003-2 C->ASexAIAhdIEKo152.2 (DNA)BsaI,BsrDIFspI,AclI,BsrDIAmpBcgIAclIXmnICspCI,PsiIFrtF (DNA),AloIFlpInR (DNA),M13-40FOR Genewiz (DNA),FspI,PvuIIBII(R),5'TR,31bp spacer,5'TR(1),5'TR,5'TR,M13F Genewiz (DNA),BssHII,BsiWI

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    7958