Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pCAGGS-CIBN-EGFP-KRasCT
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgatcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacagctagctgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattPvuISpeICAG promoterCAG enhancer20406080100120140160
    cgccaatagggactttccattgacgtcaatgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctgataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacCAG promoterCAG enhancer180200220240260280300320340
    gcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggcggggcgtcatgtagatgcataatcagtagcgataatggtacccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccgcgcgcggtccgccccNcoIBsaAISnaBICAG promoterCAG enhancer360380400420440460480500520
    cggggcgggggcgagggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctgccccgcccccgctcccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgcaacggaagcggggcacggggcgaCAG promoter540560580600620640660680700
    ccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggccctttgtgcggcgcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgagcaaagaaaagacaccgacgcactttcggaatttcccgaggccctcccgggaaacacgAfeIApaIPfoIPspOMI720740760780800820840860
    gggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcccccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgcacacgcgctcccctcgcgccggcccccgccacggggcgccacgcccccccgacgAfeIEagISacII8809009209409609801,0001,0201,040
    gaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgggcctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgccgccagcccgacattggggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcacgccccgcaccgcgccccgagcggcacggcccgBlpI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagccccctccccgcgccgccgggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgaaggaaacagggMreIBfuAIBspMIPaqCISgrAIBstAPI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgtttagacacgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgcgccccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagagggagaggtcggagccccgacaggcgcccccctgccgacKasIKasINarINarISfoISfoIPluTIPluTISacII1,4201,4401,4601,4801,5001,5201,5401,560
    ccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagAATTCACCATGAATGGAGCggaagccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagatcgatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcgaggacccgttgcacgaccaataacacgacagagtagtaaaaccgtttcTTAAGTGGTACTTACCTCGAlwNIEcoRICAG1640cag1700-seq-fwdCIBNCIBN1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TATAGGAGGTGACCTTTTGCTCAATTTTCCTGACATGTCGGTCCTAGAGCGCCAAAGGGCTCACCTCAAGTACCTCAATCCCACCTTTGATTCTCCTCTCGCCGGCTTCTTTGCCGATTCTTCAATGATTACCGGCGGCGAGATGGACAGCTATCTTTCGACTGCCGGTTTGAATATATCCTCCACTGGAAAACGAGTTAAAAGGACTGTACAGCCAGGATCTCGCGGTTTCCCGAGTGGAGTTCATGGAGTTAGGGTGGAAACTAAGAGGAGAGCGGCCGAAGAAACGGCTAAGAAGTTACTAATGGCCGCCGCTCTACCTGTCGATAGAAAGCTGACGGCCAAACTTADrdIBstEIICIBNCIBN1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CTTCCGATGATGTACGGTGAGACGACGGTGGAAGGTGATTCAAGACTCTCAATTTCGCCGGAAACGACGCTTGGGACTGGAAATTTCAAGGCAGCGAAGTTTGATACAGAGACTAAGGATTGTAATGAGGCGGCGAAGAAGATGACGATGAACAGAGATGACCTAGTAGAAGAAGGAAGGCTACTACATGCCACTCTGCTGCCACCTTCCACTAAGTTCTGAGAGTTAAAGCGGCCTTTGCTGCGAACCCTGACCTTTAAAGTTCCGTCGCTTCAAACTATGTCTCTGATTCCTAACATTACTCCGCCGCTTCTTCTACTGCTACTTGTCTCTACTGGATCATCTTCTTCAjuIBsmBIEsp3ICIBNCIBN1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GAGAAGAAGAGAAGTCGAAAATAACAGAGCAAAACAATGGGAGCACAAAAAGCATCAAGAAGATGAAACACAAAGCCAAGAAAGAAGAGAACAATTTCTCTAATGATTCATCTAAAGTGACGAAGGAATTGGAGAAAACGGATTATATTCATCTCGAGCGCGGCCGCatggtgagCTCTTCTTCTCTTCAGCTTTTATTGTCTCGTTTTGTTACCCTCGTGTTTTTCGTAGTTCTTCTACTTTGTGTTTCGGTTCTTTCTTCTCTTGTTAAAGAGATTACTAAGTAGATTTCACTGCTTCCTTAACCTCTTTTGCCTAATATAAGTAGAGCTCGCGCCGGCGtaccactcEagINotITliIXhoIXmnIPaeR7ICIBNCIBN2,1202,1402,1602,1802,2002,2202,2402,260
    caagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcaagtagacgtggtggccgttcgacgggcacgggaccgggtgggagBcgIEGFP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccccactggtgggactggatgccgcacgtcacgaagtcggcgatggggctggtgtacttcgtcgtgctgaagaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggPfoIEGFP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcaaccacttggcgtagctcgacttcccgtagctgaagttcctcctgccgttgtaggaccccgtgttcgacctcatgttgatgttgtcggtgttgcagatatagtaccggctgttcgtcttcttgccgtagttccacttgaagttctaggcggtgttgtagctcctgccgtcgcacgtEGFP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgcctctagaaagatgagcaaagatggtcgagcggctggtgatggtcgtcttgtgggggtagccgctgccggggcacgacgacgggctgttggtgatggactcgtgggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggagatctttctactcgtttctaccaXbaIBpu10IBpu10IK1M2S3K4D5G62958-3017EGFPKrasCT2,8202,8402,8602,8802,9002,9202,9402,960
    aaaaagaagaaaaagaagtcaaagacaaagtgtgtaattatgtaaGtcgacggatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcatttttcttctttttcttcagtttctgtttcacacattaatacattCagctgcctagaaaaagggagacggtttttaatacccctgtagtacttcggggaactcgtagactgaagaccgattatttcctttaaataaaagtaacgttatcacacaaccttaaaaaacacagagagtAccISalIHincIIK7K8K9K10K11K12S13K14T15K16C17V18I19M202958-3017KrasCTcaggs-seq-revCAGGS-R22,9803,0003,0203,0403,0603,0803,1003,1203,140
    ctcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagcctgagccttcctgtataccctcccgtttagtaaattttgtagtcttactcataaaccaaatctcaaaccgttgtatacgggtatacgaccgacggtacttgtttccaaccgatatttctccagtagtcatatactttgtcgggggacgacaggtaaggaataaggtatcttttcggaAjuIPflMIBstAPI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgcagcccaagcttggactgaactccaatctaaaaaaaatataaaacaaaacacaataaaaaaagaaattgtagggattttaaaaggaatgtacaaaatgatcggtctaaaaaggaggagaggactgatgagggtcagtatcgacagggagaagagaatacctctagggagctggacgtcgggttcgaaccPstIBfuAIBspMIHindIII3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtlac promoterLacOM13-rev3,5203,5403,5603,5803,6003,6203,6403,660
    gcggatccgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggagcgcctaggcgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctcBplIBplINcoISfiIBamHIBsaBISV40 Ori3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gcttttttggaggcctaggcttttgcaaaaagctaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccgctgccgaaaaaacctccggatccgaaaacgtttttcgattgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctaggcgacgBsmIPsiIStuIAvrIIBamHISV40 Ori3,8603,8803,9003,9203,9403,9603,9804,0004,020
    attaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacattaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtaSapIBsaXIBspQI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacDrdIColE1 origin4,2204,2404,2604,2804,3004,3204,3404,360
    cgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagttacgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataApaLIBciVIBseYIColE1 origin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgaAlwNIColE1 origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtColE1 origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccagcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtAmp_RColE1 origin4,9204,9404,9604,9805,0005,0205,0405,060
    tagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctcatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggagAhdIBsaIAmp_R5,0805,1005,1205,1405,1605,1805,2005,2205,240
    catccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacAclIFspIAmp_R5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtaacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatBcgIPvuIScaIAmp_R5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagAclIXmnIApaLIAmp_R5,6205,6405,6605,6805,7005,7205,7405,760
    agcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgttcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacaSspIBciVIAmp_R5,7805,8005,8205,8405,8605,8805,9005,9205,940
    atttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgtaaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggacSexAI5,9605,9806,000
    • Plasmid
    • Assembly history
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6008 bp45005000550050010001500Amp_RCAG promoterCAG enhancer

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    6008