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  • 0 feature results
pMS81 ( rpl-28::mKate2::unc-54utr ; Ch. II Integration Vector) (Github)
  • Sequence Map
    Editing disabled on read-only sequence.
    TAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTAhdIBanIBmrIBpmIBsrFIpUC57AmpR20406080100120140160
    GACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAColE1 originpUC57180200220240260280300320340
    TTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTAcuIColE1 originpUC57360380400420440460480500520
    CTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGAlwNIColE1 originpUC57540560580600620640660680700
    TACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGAHaeIIColE1 originpUC57720740760780800820840860
    GACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTPciIColE1 originpUC578809009209409609801,0001,0201,040
    TACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAEaeISapIBspQIHaeIIPvuIIpUC571,0601,0801,1001,1201,1401,1601,1801,2001,220
    TAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTcactCGTTTAGGCTATTCCCCTATTTTTGATATTCCTTCGCACATatgaaaacATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGTTCGAgtgaGCAAATCCGATAAGGGGATAAAAACTATAAGGAAGCGTGTAtacttttgBanIF14E5.8 CDSLeft Homology Armlac promoterLacOM13-revpUC571,2401,2601,2801,3001,3201,3401,3601,3801,400
    tactttttttcgaaactgtTAACTCCAGAATTTATAAATCTATAGCCCTTACTTGATTATTTATTATCATGGTTACTCACCTCAtgtctctttctcttttcaagAAGTCTTCGTTCATATCTGTTGTCTCTTTCCTCCTTTTGAATCTCTGCTTTGCTCTTCGCCAttgtttcctatgaaaaaaagctttgacaATTGAGGTCTTAAATATTTAGATATCGGGAATGAACTAATAAATAATAGTACCAATGAGTGGAGTacagagaaagagaaaagttcTTCAGAAGCAAGTATAGACAACAGAGAAAGGAGGAAAACTTAGAGACGAAACGAGAAGCGGTaacaaaggaBpmIHpaIPsiISapIBspQIBstBILeft Homology ArmF14E5.8 CDS1,4201,4401,4601,4801,5001,5201,5401,560
    gaaaataatgtAACTTGAATTGTGtaatatacttttttaatttgaatttggcTTGTAACGCGGAATCACTACGTGCGGGATCATTTCTTACTAGAAAACCCAGAAAATGCCATATTTCACTTATCTCGGGGTcatttctaattagaaaagctacaaaaaccatttctaattACGGcttttattacaTTGAACTTAACACattatatgaaaaaattaaacttaaaccgAACATTGCGCCTTAGTGATGCACGCCCTAGTAAAGAATGATCTTTTGGGTCTTTTACGGTATAAAGTGAATAGAGCCCCAgtaaagattaatcttttcgatgtttttggtaaagattaaTGCCBsaAIDraIIILeft Homology Arm1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGACTTTAGAACTGGTCGAAATAGCTAATTAGAAATGGGTTTTTGCTAATTAGAGGTGACTCTATGTTATCTGAGTAAGCCTCGTTTGTATGTAAACTCACAGTACTAATAAATGCAAGACACCCGGGTTTGTCTAGatatgaaataattgaaatatcaATTCTGACAGACAATAACTGAAATCTTGACCAGCTTTATCGATTAATCTTTACCCAAAAACGATTAATCTCCACTGAGATACAATAGACTCATTCGGAGCAAACATACATTTGAGTGTCATGATTATTTACGTTCTGTGGGCCCAAACAGATCtatactttattaactttatagtTAAGACTGTCTGTTATScaISmaIXbaIXmaITspMILeft Homology Arm1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ATGGTAATCTTGATAAGGAGTTCCACGCCCAGGAGAACACGttagttttcttgtttttgattGCGTGCgttattttggagaaaaactcgattttttacaaaataattttttgaaaggaacactgttcaataagttttgtcttttttctcaGTTGTGATAcggttttttattctttTACCATTAGAACTATTCCTCAAGGTGCGGGTCCTCTTGTGCaatcaaaagaacaaaaactaaCGCACGcaataaaacctctttttgagctaaaaaatgttttattaaaaaactttccttgtgacaagttattcaaaacagaaaaaagagtCAACACTATgccaaaaaataagaaaLeft Homology Arm1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tttgtaGTTATACAGAAGACCGcctgcaggATTTGAGTAGAGCACTCGAGGGGAGAAAATGCGGAGAGAAGAACCCAGCATCTTAATAAAATTATATAATTTTGAATAAAATCAAATTAAAAATAAGATTAATTGAAACACAACAATAGAATGCAGAAAATTACTCATAAGAAATaaacatCAATATGTCTTCTGGCggacgtccTAAACTCATCTCGTGAGCTCCCCTCTTTTACGCCTCTCTTCTTGGGTCGTAGAATTATTTTAATATATTAAAACTTATTTTAGTTTAATTTTTATTCTAATTAACTTTGTGTTGTTATCTTACGTCTTTTAATGAGTATTCTTTABsmIPstISbfITliIXhoIPspXIPaeR7Irpl-28 PromoterLeft Homology Arm2,1202,1402,1602,1802,2002,2202,2402,260
    AATTTATTTTGAAAGAAGTTGTGTCGATTTACGATAAAAACTACACGCAAACGAGGCTCCGCGTTTACACCGTTTGGCAATGGAGCGCGCTTGCTATTAGATTTTAGGCCAGCTCAACGAAAGTTTAATTTATTTTTCTTCTTTTTTTCCATTTTTTTGAGTAAAATATCGAATTTTAAATAAAACTTTCTTCAACACAGCTAAATGCTATTTTTGATGTGCGTTTGCTCCGAGGCGCAAATGTGGCAAACCGTTACCTCGCGCGAACGATAATCTAAAATCCGGTCGAGTTGCTTTCAAATTAAATAAAAAGAAGAAAAAAAGGTAAAAAAACTCATTTTATAGCTTAABssHIIrpl-28 Promoter2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TTTTGTATTAATTCTGTAAATTTTATAATTTGCAACTATTTTGGCGTATTCTGCTTGTTTTTATCAGCGAATTTTTATGCATTTCAGTCAAAAGTTTGAGCAGGAAAAATGACATTTAGGACTTTGTAAAATTGTATTCTTAAATTTGCTTGAAAACCTACGTGTACAATTGATTAAAACATAATTAAGACATTTAAAATATTAAACGTTGATAAAACCGCATAAGACGAACAAAAATAGTCGCTTAAAAATACGTAAAGTCAGTTTTCAAACTCGTCCTTTTTACTGTAAATCCTGAAACATTTTAACATAAGAATTTAAACGAACTTTTGGATGCACATGTTAACTAAMfeIPsiIBsaAIBsrGIrpl-28 Promoter2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TTATTGAATTTTTCTTACGTTTCCCGTTGTATCTTATTGTATTTAACTTTCATCATAAAATAATTTATTTCCAGGTAAAATATCCGACGCTCTCGTGATGGTCTCCGAGCTCATTAAAGAAAACATGCATATGAAGCTCTACATGGAGGGAACCGTCAACAACCACCACTTCAAGAATAACTTAAAAAGAATGCAAAGGGCAACATAGAATAACATAAATTGAAAGTAGTATTTTATTAAATAAAGGTCCATTTTATAGGCTGCGAGAGCACTACCAGAGGCTCGAGTAATTTCTTTTGTACGTATACTTCGAGATGTACCTCCCTTGGCAGTTGTTGGTGGTGAAGTTCrpl-28 PromoterSynthetic mKate2 Gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TGCACCTCCGAGGGAGAGGGAAAGCCATACGAGGGAACCCAAACCATGCGTATCAAGGCCGTCGAGGGAGGACCACTCCCATTCGCCTTCGACATCCTCGCCACCTCCTTCATGTACGGATCCAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcACGTGGAGGCTCCCTCTCCCTTTCGGTATGCTCCCTTGGGTTTGGTACGCATAGTTCCGGCAGCTCCCTCCTGGTGAGGGTAAGCGGAAGCTGTAGGAGCGGTGGAGGAAGTACATGCCTAGGTTCcattcaaatttgtatatatatgattgattgggactaataaatttaaaagBamHISynthetic mKate2 GeneSynthetic Intron2,8202,8402,8602,8802,9002,9202,9402,960
    agACCTTCATCAACCACACCCAAGGAATCCCAGACTTCTTCAAGCAATCCTTCCCAGAGGGATTCACCTGGGAGCGTGTCACCACCTACGAGGACGGAGGAGTCCTCACCGCCACCCAAGACACCTCCCTCCAAGACGGATGCCTCATCTACAACGTCAAGgtaagtttaaacagtcTGGAAGTAGTTGGTGTGGGTTCCTTAGGGTCTGAAGAAGTTCGTTAGGAAGGGTCTCCCTAAGTGGACCCTCGCACAGTGGTGGATGCTCCTGCCTCCTCAGGAGTGGCGGTGGGTTCTGTGGAGGGAGGTTCTGCCTACGGAGTAGATGTTGCAGTTCcattcaaatttgtcSynthetic mKate2 GeneSynt...ron2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ttcggtactaactaaccatacatatttaaattttcagATCCGTGGAGTCAACTTCCCATCCAACGGACCAGTCATGCAAAAGAAGACCCTCGGATGGGAGGCCTCCACCGAGACCCTCTACCCAGCCGACGGAGGACTTGAGGGACGTGCCGACATGGCCCTCAAGCTCGTCGGAaagccatgattgattggtatgtataaatttaaaagtcTAGGCACCTCAGTTGAAGGGTAGGTTGCCTGGTCAGTACGTTTTCTTCTGGGAGCCTACCCTCCGGAGGTGGCTCTGGGAGATGGGTCGGCTGCCTCCTGAACTCCCTGCACGGCTGTACCGGGAGTTCGAGCAGCCTBglIBtgIStuISynthetic mKate2 GeneSynthetic intron3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GGAGGACACCTCATCTGCAACCTCAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagACCACCTACCGTTCCAAGAAGCCAGCCAAGAACCTCAAGATGCCAGGAGTCTACTACGTCGACCGTCGTCTTGAGCGTATCAAGGAGGCCGACAAGGCCTCCTGTGGAGTAGACGTTGGAGTTCcattcaaatttgtactaaaatgattgattgattagactaaatttaaaagtcTGGTGGATGGCAAGGTTCTTCGGTCGGTTCTTGGAGTTCTACGGTCCTCAGATGATGCAGCTGGCAGCAGAACTCGCATAGTTCCTCCGGCTGTTCCSalIPshAISynthetic mKate2 GeneSynthetic intron3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AGACCTACGTCGAGCAACACGAGGTCGCCGTCGCCCGTTACTGCGACCTCCCATCCAAGCTCGGACACCGTTAAGTCCAATTACTCTTCAACATCCCTACATGCTCTTTCTCCCTGTGCTCCCACCCCCTATTTTTGTTATTATCAAAAAACTTCTCTTAATTTCTTTGTTTTTTTCTGGATGCAGCTCGTTGTGCTCCAGCGGCAGCGGGCAATGACGCTGGAGGGTAGGTTCGAGCCTGTGGCAATTCAGGTTAATGAGAAGTTGTAGGGATGTACGAGAAAGAGGGACACGAGGGTGGGGGATAAAAACAATAATAGTTTTTTGAAGAGAATTAAAGAAACAAAAAAPshAIunc-54 3'UTRSynthetic mKate2 Gene3,5203,5403,5603,5803,6003,6203,6403,660
    AGCTTCTTTTAAGTCACCTCTAACAATGAAATTGTGTAGATTCAAAAATAGAATTAATTCGTAATAAAAAGTCGAAAAAAATTGTGCTCCCTCCCCCCATTAATAATAATTCTATCCCAAAATCTACACAATGTTCTGTGTACACTTCTTATGTTTTTTACTTCTGATAAATTTTTCGAAGAAAATTCAGTGGAGATTGTTACTTTAACACATCTAAGTTTTTATCTTAATTAAGCATTATTTTTCAGCTTTTTTTAACACGAGGGAGGGGGGTAATTATTATTAAGATAGGGTTTTAGATGTGTTACAAGACACATGTGAAGAATACAAAAAATGAAGACTATTTAAAAXmnIBsrGIunc-54 3'UTR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TTTGAAACATCATAGAAAAAACCGCACACAAAATACCTTATCATATGTTACGTTTCAGTTTATGACCGCAATTTTTACTTAAGTTCAATCCGGAAAGgtaagtttaaaATAACTTCGTATAGCATACATTATACGAAGTTATtttcagGGAGAGCAAAAGATTTTTGCTTTCGTCAAACTTTGTAGTATCTTTTTTGGCGTGTGTTTTATGGAATAGTATACAATGCAAAGTCAAATACTGGCGTTAAAAATGAATTCAAGTTAGGCCTTTCcattcaaatttTATTGAAGCATATCGTATGTAATATGCTTCAATAaaagtcCCTCTCGTTTTCTAAAAACGAAAGCAGAflIIBspEIunc-54 3'UTRLoxPPrps-0Sy...)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    GTAAATCTACACACGCGTCTCTTCCGTGCGAGAGTCCAAGCCAGCAGCCAAATTCGTTGACTGAGTATTCAACGTTTATACGTTGTCGGCAACGAGAAATAGGAAAATGCATCGGGAAATGTTCTTTTTTCGATTTTTTCCAAGGTTTTGACAAATTTTACCACGAATTTTGCTACATTTAGATGTGTGCGCAGAGAAGGCACGCTCTCAGGTTCGGTCGTCGGTTTAAGCAACTGACTCATAAGTTGCAAATATGCAACAGCCGTTGCTCTTTATCCTTTTACGTAGCCCTTTACAAGAAAAAAGCTAAAAAAGGTTCCAAAACTGTTTAAAATGGTGCTTAAAACGATMluIPrps-04,0404,0604,0804,1004,1204,1404,1604,1804,200
    TGTTTTCAATTAAAAAATATGTTATTCAACTGTTTCTATGAGGAAAATAAGGCTTTGCATGTAATTTTCTTATTCAGCATAATTTTTAATTAATTTGAATTTTCTGTCCTAACGTTTATTTTGTTTTCTTGGTTATGACTGATCTGAAATTAATTTTTGAATTTTAAGGTAATatACAAAAGTTAATTTTTTATACAATAAGTTGACAAAGATACTCCTTTTATTCCGAAACGTACATTAAAAGAATAAGTCGTATTAAAAATTAATTAAACTTAAAAGACAGGATTGCAAATAAAACAAAAGAACCAATACTGACTAGACTTTAATTAAAAACTTAAAATTCCATTAtaPacICspCIM1Prps-04,2204,2404,2604,2804,3004,3204,3404,360
    gaaaaaacccgagttgaccgccacatccgtagagaagttcctcatcgagaagttcgactccgtctccgacctcatgcaactctccgagggagaggagtcccgtgccttctccttcgacgtcggaggacgtggatacgtcctccgtgtcaactcctgcgccgacggattctacaagcttttttgggctcaactggcggtgtaggcatctcttcaaggagtagctcttcaagctgaggcagaggctggagtacgttgagaggctccctctcctcagggcacggaagaggaagctgcagcctcctgcacctatgcaggaggcacagttgaggacgcggctgcctaagatgttcZraIAatIIPflFITth111IK2K3P4E5L6T7A8T9S10V11E12K13F14L15I16E17K18F19D20S21V22S23D24L25M26Q27L28S29E30G31E32E33S34R35A36F37S38F39D40V41G42G43R44G45Y46V47L48R49V50N51S52C53A54D55G56F57Y58K594374-4550Hygromycin Coding Sequence4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gaccgttacgtctaccgtcacttcgcctccgccgccctcccaatcccagaggtcctcgacatcggagagttctccgagtccctcacctactgcatctcccgtcgtgcccaaggagtcaccctccaagacctcccagagaccgagctcccagccgtcctccaaccagtcgccgaggctggcaatgcagatggcagtgaagcggaggcggcgggagggttagggtctccaggagctgtagcctctcaagaggctcagggagtggatgacgtagagggcagcacgggttcctcagtgggaggttctggagggtctctggctcgagggtcggcaggaggttggtcagcggctccBtgZIPpuMIEcoO109IDR2YV4YR6HF8AS10AA12LP14IP16EV18LD20IG22EF24SE26SL28TY30CI32SR34RA36QG38VT40LQ42DL44PE46TE48LP50AV52LQ54PV56AE584551-5053Hygromycin Coding SequenceRight Homology Arm4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ccatggacgccatcgccgccgccgacctctcccaaacctccggattcggaccattcggaccacaaggaatcggacaatacaccacctggcgtgacttcatctgcgccatcgccgacccacacgtctaccactggcaaaccgtcatggacgacaccgtctccgcctccgtcgcccaggtacctgcggtagcggcggcggctggagagggtttggaggcctaagcctggtaagcctggtgttccttagcctgttatgtggtggaccgcactgaagtagacgcggtagcggctgggtgtgcagatggtgaccgtttggcagtacctgctgtggcagaggcggaggcagcgggtBtgINcoIBspEIBtgZIPflFITth111IAM60DA62IA64AA66DL68SQ70TS72GF74GP76FG78PQ80GI82GQ84YT86TW88RD90FI92CA94IA96DP98HV100YH102WQ104TV106MD108DT110VS112AS114VA116Q4551-5053Hygromycin Coding SequenceRight Homology Arm4,7404,7604,7804,8004,8204,8404,8604,8804,900
    agccctcgacgagctcatgctctgggccgaggactgcccagaggtccgtcacctcgtccacgccgacttcggatccaacaacgtcctcaccgacaacggacgtatcaccgccgtcatcgactggtccgaggccatgttcggagactcccaataTGCGGTATTTTCTCCTTACGCAtcgggagctgctcgagtacgagacccggctcctgacgggtctccaggcagtggagcaggtgcggctgaagcctaggttgttgcaggagtggctgttgcctgcatagtggcggcagtagctgaccaggctccggtacaagcctctgagggttatACGCCATAAAAGAGGAATGCGTBaeIBamHIA118LD120EL122ML124WA126ED128CP130EV132RH134LV136HA138DF140GS142NN144VL146TD148NG150RI152TA154VI156DW158SE160AM162FG164DS166Q4551-5053Hygromycin Coding SequenceRight Homology ArmGibson overlapPrimerPrimerpUC574,9204,9404,9604,9805,0005,0205,0405,060
    TCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTAGACACGCCATAAAGTGTGGCGTATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAPfoIBstAPIpUC575,0805,1005,1205,1405,1605,1805,2005,2205,240
    CAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACAZraIAatIIEcoO109IAm...rpUC575,2605,2805,3005,3205,3405,3605,3805,4005,420
    ATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCSspIAmpR promoterpUC575,4405,4605,4805,5005,5205,5405,5605,5805,600
    TGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCAcuIBcgIXmnIpUC57AmpR5,6205,6405,6605,6805,7005,7205,7405,760
    AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGEaeIPvuIScaIpUC57AmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAFspIpUC57AmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTBglIpUC57AmpR6,1406,160
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6176 bp45005000550050010001500Left Homology ArmRight Homology ArmF14E5.8 CDSPrimerGibson overlapPrimerpUC57AmpR4551-5053MS262 (DNA)MS66 (DNA)ZCS343 (DNA)MS580 (DNA)MS834 (DNA)ZCS87 (DNA)MS355 (DNA)MS78 (DNA)MS70 (DNA)MS439 (DNA)MS119 (DNA)MS425 (DNA)MS749 (DNA)ZCS318 (DNA)MS611 (DNA)MS490 (DNA)MS547 (DNA)ZCS276 (DNA)MS426 (DNA)MS271 (DNA)MS272 (DNA)MS595 (DNA)

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    6176