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GeneArt® CRISPR Nuclease Vector - OFP
  • Sequence Map
    Editing disabled on read-only sequence.
    GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCATTGGAAGAAGAGCAAAGGGTTTTTTCTAGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTACAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGAACTTTTTCACCGTGGCTCAGCCACGTAACCTTCTTCTCGTTTCCCAAAAAAGATCATATGGCAGCTGGAGATCGATCTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATBstZ17IgRNA scaffoldcrRNA20406080100120140160
    TCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCA180200220240260280300320340
    TTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATPciI360380400420440460480500520
    AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATATTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATori540560580600620640660680700
    CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTori720740760780800820840860
    CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAori8809009209409609801,0001,0201,040
    CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCori1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCAhdIAmpR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCBsrDIAmpR1,4201,4401,4601,4801,5001,5201,5401,560
    TAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAAclIFspIBsrDIAmpR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATPvuIAmpR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCAclIAmpR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGAmpR promoterAmpR2,1202,1402,1602,1802,2002,2202,2402,260
    ATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCTAAAGGGGCTTTTCACGGTGGACTGCAGCTGCCTAGCCCTCTAGAGGGCTAGGGGATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCATCACGCGCTCGTTTTAAATTCGATGTTGTTCCGSgrDI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTTCCGAACTGGCTGTTAACGTACTTCTTAGACGAATCCCAATCCGCAAAACGCGACGAAGCGCTACATGCCCGGTCTATATGCGCAACTGTAACTAATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTGAMfeIMluINruISpeICMV enhancer2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCNdeICMV enhancer2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTANcoICspCISnaBICMV enhancerCMV promoter2,8202,8402,8602,8802,9002,9202,9402,960
    TTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCSacIEco53kIR1L2V3N4R5Q6I7A8W9R103122-3216CMV promoter2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGGAGCGGCCGGCCGCCATTGGAAGAAGAGCAAAGGGGTTTTCGGCCGACCATGGGCAAGCCCATCCCTAACCCCCTGTTGGGGCTGGACAGCACCGCTCCCAAAAAGAAAAGGAAGGTGTGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTGTGGCCCTGGCTAGGTCGGAGGCCTCGCCGGCCGGCGGTAACCTTCTTCTCGTTTCCCCAAAAGCCGGCTGGTACCCGTTCGGGTAGGGATTGGGGGACAACCCCGACCTGTCGTGGCGAGGGTTTTTCTTTTCCTTCCACBbsIFseINcoIBspEIRP2PL4EE6EQ8RG10FR12PT14MG16KP18IP20NP22LL24GL26DS28TA30PK32KK34RK36V3215-8231R11H12P13R14C15F16D17L18H19R20R21H22R23D24R25S26S27L28R29S30G313122-3216V5 tagCRISPR RNASV40 NLS3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GGCATTCACGGCGTGCCTGGACAAAAAGTACAGCATCGGCCTTGATATCGGCACCAATAGCGTGGGCTGGGCCGTTATCACAGACGAATACAAGGTACCCAGCAAGAAGTTCAAGGTGCTGGGGAATACAGACAGGCACTCTATCAAGAAAAACCTTATCGGGGCTCTGCTGTTTCCGTAAGTGCCGCACGGACCTGTTTTTCATGTCGTAGCCGGAACTATAGCCGTGGTTATCGCACCCGACCCGGCAATAGTGTCTGCTTATGTTCCATGGGTCGTTCTTCAAGTTCCACGACCCCTTATGTCTGTCCGTGAGATAGTTCTTTTTGGAATAGCCCCGAGACGACAAAKpnIBstXIAcc65IG38IH40GV42PG44QK46VQ48HR50P*52YR54HQ56*R58GL60GR62YH64RR66IQ68GT70QQ72EV74QG76AG78EY80RQ82AL84YQ86EK88PY90RG92SA94V3215-8231Cas93,3403,3603,3803,4003,4203,4403,4603,4803,500
    GACTCAGGCGAGACCGCCGAGGCCACCAGGTTGAAGAGGACCGCAAGGCGAAGGTACACCCGGAGGAAGAACAGGATCTGCTATCTGCAGGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGGCTGGAGGAGAGCTTCCTTGTCGAGGAGGATAAGACTGAGTCCGCTCTGGCGGCTCCGGTGGTCCAACTTCTCCTGGCGTTCCGCTTCCATGTGGGCCTCCTTCTTGTCCTAGACGATAGACGTCCTCTAGAAGTCGTTGCTCTACCGGTTCCACCTGCTGTCGAAGAAGGTGTCCGACCTCCTCTCGAAGGAACAGCTCCTCCTATTCTPflMISexAI*96LR98RD100RR102GH104QV106EE108DR110KA112KV114HP116EE118EQ120DL122LS124AG126DL128QQ130RD132GQ134GG136RQ138LL140PQ142AG144GE146LP148CR150GG152*E1543215-8231Cas93,5203,5403,5603,5803,6003,6203,6403,660
    AGCACGAACGACACCCCATCTTCGGCAACATAGTCGACGAGGTCGCTTATCACGAGAAGTACCCCACCATCTACCACCTGCGAAAGAAATTGGTGGATAGCACCGATAAAGCCGACTTGCGACTTATCTACTTGGCTCTGGCGCACATGATTAAGTTCAGGGGCCACTTCCTGATTCGTGCTTGCTGTGGGGTAGAAGCCGTTGTATCAGCTGCTCCAGCGAATAGTGCTCTTCATGGGGTGGTAGATGGTGGACGCTTTCTTTAACCACCTATCGTGGCTATTTCGGCTGAACGCTGAATAGATGAACCGAGACCGCGTGTACTAATTCAAGTCCCCGGTGAAGGACTAPaqCIPflFITth111IAR156TT158PH160LR162QH164SR166RG168RL170SR172EV174PH176HL178PP180AK182EI184GG186*H188R*190SR192LA194TY196LL198GS200GA202HD204*V206QG208PL210PD2123215-8231Cas93,6803,7003,7203,7403,7603,7803,8003,8203,840
    CGAGGGCGACCTTAACCCCGACAACAGTGACGTAGACAAATTGTTCATCCAGCTTGTACAGACCTATAACCAGCTGTTCGAGGAAAACCCTATTAACGCCAGCGGGGTGGATGCGAAGGCCATACTTAGCGCCAGGCTGAGCAAAAGCAGGCGCTTGGAGAACCTGATAGCCCAGGCTCCCGCTGGAATTGGGGCTGTTGTCACTGCATCTGTTTAACAAGTAGGTCGAACATGTCTGGATATTGGTCGACAAGCTCCTTTTGGGATAATTGCGGTCGCCCCACCTACGCTTCCGGTATGAATCGCGGTCCGACTCGTTTTCGTCCGCGAACCTCTTGGACTATCGGGTCBlpIBsrGIBstXIRG214RP216*P218RQ220Q*222RR224QI226VH228PA230CT232DL234*P236AV238RG240KP242Y*244RQ246RG248GC250EG252HT254*R256QA258EQ260KQ262AL264GE266PD268SP2703215-8231Cas93,8603,8803,9003,9203,9403,9603,9804,0004,020
    CTGCCCGGTGAAAAGAAGAACGGCCTCTTCGGTAATCTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCAGAAGATGCCAAGCTGCAGTTGAGTAAGGACACCTATGACGACGACTTGGACAATCTGCTCGCCCAAATCGGCGACCAGTACGGACGGGCCACTTTTCTTCTTGCCGGAGAAGCCATTAGACTAACGGGACTCGGACCCGGACTGGGGGTTGAAGTTCTCGTTGAAGCTGGACCGTCTTCTACGGTTCGACGTCAACTCATTCCTGTGGATACTGCTGCTGAACCTGTTAGACGAGCGGGTTTAGCCGCTGGTCATGCAA272R*274KE276ER278PL280R*282SD284CP286EP288GP290DP292QL294QE296QL298RP300GR302RC304QA306AV308E*310GH312L*314RR316LG318QS320AR322PN324RR326PV328R3215-8231Cas94,0404,0604,0804,1004,1204,1404,1604,1804,200
    CTGACCTGTTCCTCGCCGCCAAGAACCTTTCTGACGCAATCCTGCTTAGCGATATCCTTAGGGTGAACACAGAGATCACCAAGGCCCCCCTGAGCGCCAGCATGATCAAGAGGTACGACGAGCACCATCAGGACCTGACCCTTCTGAAGGCCCTGGTGAGGCAGCAACTGCCCGAGACTGGACAAGGAGCGGCGGTTCTTGGAAAGACTGCGTTAGGACGAATCGCTATAGGAATCCCACTTGTGTCTCTAGTGGTTCCGGGGGGACTCGCGGTCGTACTAGTTCTCCATGCTGCTCGTGGTAGTCCTGGACTGGGAAGACTTCCGGGACCACTCCGTCGTTGACGGGCTBlpIBsu36I*330PV332PR334RQ336EP338F*340RN342PA344*R346YP348*G350EH352RD354HQ356GP358PE360RQ362HD364QE366VR368RA370PS372GP374DP376SE378GP380GE382AA384TA386R3215-8231Cas94,2204,2404,2604,2804,3004,3204,3404,360
    GAAGTACAAGGAGATCTTTTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATCGACGGCGGAGCCAGCCAAGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGATGGCACCGAGGAGCTGCTGGTGAAGCTGAACAGGGAAGATTTGCTCCGGAAGCAGAGGCTTCATGTTCCTCTAGAAAAAGCTGGTCTCGTTCTTGCCGATGCGGCCGATGTAGCTGCCGCCTCGGTCGGTTCTCCTCAAGATGTTCAAGTAGTTCGGGTAGGACCTCTTCTACCTACCGTGGCTCCTCGACGACCACTTCGACTTGTCCCTTCTAAACGAGGCCTTCGTCTCCBspEIE388VQ390GD392LF394RP396EQ398ER400LR402RL404HR406RR408SQ410PR412GV414LQ416VH418QA420HP422GE424DG426WH428RG430AA432GE434AE436QG438RF440AP442EA444E3215-8231Cas94,3804,4004,4204,4404,4604,4804,5004,5204,540
    ACCTTTGACAACGGTAGCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCAATACTGAGGCGACAGGAGGATTTCTACCCCTTCCTCAAGGACAATAGGGAGAAAATCGAAAAGATTCTGACCTTCAGGATCCCCTACTACGTGGGCCCTCTTGCCAGGGGCAACAGCCGATTGGAAACTGTTGCCATCGTAGGGGGTGGTCTAGGTGGACCCGCTCGACGTGCGTTATGACTCCGCTGTCCTCCTAAAGATGGGGAAGGAGTTCCTGTTATCCCTCTTTTAGCTTTTCTAAGACTGGAAGTCCTAGGGGATGATGCACCCGGGAGAACGGTCCCCGTTGTCGGCTAApaIBamHIPspOMID446L*448QR450*H452PP454PD456PP458GR460AA462RN464TE466AT468GG470FL472PL474PQ476GQ478*G480EN482RK484DS486DL488QD490PL492LR494GP496SC498QG500QQ502PI5043215-8231Cas94,5604,5804,6004,6204,6404,6604,6804,7004,720
    TCGCTTGGATGACAAGAAAGAGCGAGGAGACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAAGGAGCAAGCGCGCAGTCTTTCATCGAACGGATGACCAATTTCGACAAAAACCTGCCTAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTTTACGAGTACTTCACCGTAGCGAACCTACTGTTCTTTCTCGCTCCTCTGGTAGTGGGGGACCTTGAAGCTCCTTCACCACCTGTTTCCTCGTTCGCGCGTCAGAAAGTAGCTTGCCTACTGGTTAAAGCTGTTTTTGGACGGATTGCTCTTCCACGACGGGTTCGTGTCGGACGAAATGCTCATGAAGTGGCABssHIIRL506DD508KK510ER512GD514HH516PL518EL520RG522SG524GQ526RS528KR530AV532FH534RT536DD538QF540RQ542KP544A*546RE548GA550AQ552AQ554PA556LR558VL560HR5623215-8231Cas94,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTACAACGAGCTCACCAAGGTGAAATATGTGACCGAGGGCATGCGAAAACCCGCTTTCCTGAGCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACCAACAGGAAGGTGACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTTGATAGCGTGCATGTTGCTCGAGTGGTTCCACTTTATACACTGGCTCCCGTACGCTTTTGGGCGAAAGGACTCGCCGCTCGTCTTCTTCCGGTAGCACCTGGACGACAAGTTCTGGTTGTCCTTCCACTGGCACTTCGTCGACTTCCTCCTGATGAAGTTCTTCTAGCTCACGAAACTATCGCACAleIBarISacISphIBsrGIBstEIIEco53kIVQ564RA566HQ568GE570IC572DR574GH576AK578TR580FP582ER584RA586EE588GH590RG592PA594VQ596DQ598QE600GD602RE604AA606EG608GL610LQ612ED614RV616L*618*R6203215-8231Cas94,9204,9404,9604,9805,0005,0205,0405,060
    GAAATAAGCGGCGTGGAGGACAGGTTCAACGCCAGCCTGGGCACCTACCACGACTTGTTGAAGATAATCAAAGACAAGGATTTCCTGGATAATGAGGAGAACGAGGATATACTCGAGGACATCGTGCTGACTTTGACCCTGTTTGAGGACCGAGAGATGATTGAAGAAAGGCTCACTTTATTCGCCGCACCTCCTGTCCAAGTTGCGGTCGGACCCGTGGATGGTGCTGAACAACTTCTATTAGTTTCTGTTCCTAAAGGACCTATTACTCCTCTTGCTCCTATATGAGCTCCTGTAGCACGACTGAAACTGGGACAAACTCCTGGCTCTCTACTAACTTCTTTCCGAGTTliIXhoIPspXIPaeR7IGN622KR624RG626GQ628VQ630RQ632PG634HL636PR638LV640ED642NQ644RQ646GF648PG650**652GE654RG656YT658RG660HR662AD664FD666PV668*G670PR672DD674*R676KA678Q3215-8231Cas95,0805,1005,1205,1405,1605,1805,2005,2205,240
    AAACCTACGCCCACCTGTTCGACGACAAAGTGATGAAACAACTGAAGAGACGAAGATACACCGGCTGGGGCAGACTGTCCAGGAAGCTCATCAACGGCATTAGGGACAAGCAGAGCGGCAAGACCATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACCGAAACTTCATGCATTTGGATGCGGGTGGACAAGCTGCTGTTTCACTACTTTGTTGACTTCTCTGCTTCTATGTGGCCGACCCCGTCTGACAGGTCCTTCGAGTAGTTGCCGTAATCCCTGTTCGTCTCGCCGTTCTGGTAGGACCTAAAGGACTTCAGGCTGCCGAAGCGGTTGGCTTTGAAGTACGTN680LR682PP684VR686RQ688SD690ET692TE694ET696KI698HR700LG702QT704VQ706EA708HQ710RH712*G714QA716ER718QD720HP722GF724PE726VR728RL730RQ732PK734LH736A3215-8231Cas95,2605,2805,3005,3205,3405,3605,3805,4005,420
    GCTGATTCACGATGACAGCTTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTTAGCGGCCAGGGCGACTCCCTGCACGAACATATTGCAAACCTGGCAGGCTCCCCTGCGATCAAGAAGGGCATACTGCAGACCGTTAAGGTTGTGGACGAATTGGTCAAGGTCATGGGCAGGCGACTAAGTGCTACTGTCGAACTGGAAGTTCCTCCTGTAGGTCTTCCGGGTCCAATCGCCGGTCCCGCTGAGGGACGTGCTTGTATAACGTTTGGACCGTCCGAGGGGACGCTAGTTCTTCCCGTATGACGTCTGGCAATTCCAACACCTGCTTAACCAGTTCCAGTACCCGTCCAjuIA738DS740R*742QL744DL746QG748GH750PE752GP754G*756RP758GR760LP762AR764TY766CK768PG770RL772PC774DQ776EG778HT780AD782R*784GC786GR788IG790QG792HG794Q3215-8231Cas95,4405,4605,4805,5005,5205,5405,5605,5805,600
    CACAAGCCCGAAAACATAGTTATAGAGATGGCCAGAGAGAACCAGACCACCCAAAAGGGCCAGAAGAACAGCCGGGAGCGCATGAAAAGGATCGAGGAGGGTATCAAGGAACTCGGAAGCCAGATCCTCAAAGAGCACCCCGTGGAGAATACCCAGCTCCAGAACGAGAAGCTGTGTGTTCGGGCTTTTGTATCAATATCTCTACCGGTCTCTCTTGGTCTGGTGGGTTTTCCCGGTCTTCTTGTCGGCCCTCGCGTACTTTTCCTAGCTCCTCCCATAGTTCCTTGAGCCTTCGGTCTAGGAGTTTCTCGTGGGGCACCTCTTATGGGTCGAGGTCTTGCTCTTCGACAA796QA798RK800HS802YR804DG806QR808EP810DH812PK814GP816EE818QP820GA822HE824KD826RG828GY830QG832TR834KP836DP838QR840AP842RG844EY846PA848PE850RE852AV8543215-8231Cas95,6205,6405,6605,6805,7005,7205,7405,760
    ACCTGTACTACCTGCAGAACGGCAGGGACATGTACGTTGACCAGGAGTTGGACATCAACAGGCTTTCAGACTATGACGTGGATCACATAGTGCCCCAGAGCTTTCTTAAAGACGATAGCATCGACAACAAGGTCCTGACCCGCTCCGACAAAAACAGGGGCAAAAGCGACAACGTTGGACATGATGGACGTCTTGCCGTCCCTGTACATGCAACTGGTCCTCAACCTGTAGTTGTCCGAAAGTCTGATACTGCACCTAGTGTATCACGGGGTCTCGAAAGAATTTCTGCTATCGTAGCTGTTGTTCCAGGACTGGGCGAGGCTGTTTTTGTCCCCGTTTTCGCTGTTGCAPciIBmgBIPflMIPV856LP858AE860RQ862GH864VR866*P868GV870GH872QQ874AF876RL878*R880GS882HS884AP886EL888S*890RR892*H894RQ896QG898PD900PL902RQ904KQ906GQ908KR910QR9123215-8231Cas95,7805,8005,8205,8405,8605,8805,9005,9205,940
    GCCAAGCGAAGAGGTGGTTAAAAAGATGAAGAACTACTGGAGGCAACTGCTCAACGCGAAATTGATCACCCAGAGAAAGTTCGATAACCTGACCAAGGCCGAGAGGGGCGGACTCTCCGAACTTGACAAAGCGGGCTTCATAAAGAGGCAGCTGGTCGAGACCCGACAGATCACGCGGTTCGCTTCTCCACCAATTTTTCTACTTCTTGATGACCTCCGTTGACGAGTTGCGCTTTAACTAGTGGGTCTCTTTCAAGCTATTGGACTGGTTCCGGCTCTCCCCGCCTGAGAGGCTTGAACTGTTTCGCCCGAAGTATTTCTCCGTCGACCAGCTCTGGGCTGTCTAGTGCAK914RR916GG918*K920DE922EL924LE926AT928AQ930RE932ID934HP936EK938VR940*P942DQ944GR946EG948RT950LR952T*954QS956GL958HK960EA962AG964RD966PT968DH9703215-8231Cas95,9605,9806,0006,0206,0406,0606,0806,1006,120
    AAGCACGTGGCCCAAATCCTCGACAGCAGAATGAATACCAAGTACGATGAGAATGACAAACTCATCAGGGAAGTGAAAGTGATTACCCTGAAGAGCAAGTTGGTGTCCGACTTTCGCAAAGATTTCCAGTTCTACAAGGTGAGGGAGATCAACAACTACCACCATGCCCACGACGTTCGTGCACCGGGTTTAGGAGCTGTCGTCTTACTTATGGTTCATGCTACTCTTACTGTTTGAGTAGTCCCTTCACTTTCACTAATGGGACTTCTCGTTCAACCACAGGCTGAAAGCGTTTCTAAAGGTCAAGATGTTCCACTCCCTCTAGTTGTTGATGGTGGTACGGGTGCTGCAjuIPmlIEA972RG974PN976PR978QQ980NE982YQ984VR986*E988*Q990TH992QG994SE996SD998YP1000EE1002QV1004GV1006RL1008SQ1010RF1012PV1014LQ1016GE1018GD1020QQ1022LP1024PC1026PR1028R3215-8231Cas96,1406,1606,1806,2006,2206,2406,2606,2806,300
    CATACCTGAACGCCGTGGTCGGCACCGCCCTGATTAAGAAGTATCCAAAGCTGGAGTCCGAATTTGTCTACGGCGACTACAAAGTTTACGATGTGAGGAAGATGATCGCTAAGAGCGAACAGGAGATCGGCAAGGCCACCGCTAAGTATTTCTTCTACAGCAACATCATGAACTTGTATGGACTTGCGGCACCAGCCGTGGCGGGACTAATTCTTCATAGGTTTCGACCTCAGGCTTAAACAGATGCCGCTGATGTTTCAAATGCTACACTCCTTCTACTAGCGATTCTCGCTTGTCCTCTAGCCGTTCCGGTGGCGATTCATAAAGAAGATGTCGTTGTAGTACTTGAAI1030PE1032RR1034GR1036HR1038PD1040*E1042VS1044KA1046GV1048RI1050CL1052RR1054LQ1056SL1058RC1060EE1062DD1064R*1066ER1068TG1070DR1072QG1074HR1076*V1078FL1080LQ1082QH1084HE1086L3215-8231Cas96,3206,3406,3606,3806,4006,4206,4406,460
    TTTCAAGACCGAGATCACACTTGCCAACGGCGAAATCAGGAAGAGGCCGCTTATCGAGACCAACGGTGAGACCGGCGAGATCGTGTGGGACAAGGGCAGGGACTTCGCCACCGTGAGGAAAGTCCTGAGCATGCCCCAGGTGAATATTGTGAAAAAAACTGAGGTGCAGACAGGCAAAGTTCTGGCTCTAGTGTGAACGGTTGCCGCTTTAGTCCTTCTCCGGCGAATAGCTCTGGTTGCCACTCTGGCCGCTCTAGCACACCCTGTTCCCGTCCCTGAAGCGGTGGCACTCCTTTCAGGACTCGTACGGGGTCCACTTATAACACTTTTTTTGACTCCACGTCTGTCCGSphIF1088QD1090RD1092HT1094CQ1096RR1098NQ1100EE1102AA1104YR1106DQ1108R*1110DR1112RD1114RV1116GQ1118GQ1120GL1122RH1124RE1126ES1128PE1130HA1132PG1134EY1136CE1138KN1140*G1142AD1144R3215-8231Cas96,4806,5006,5206,5406,5606,5806,6006,6206,640
    GGCTTTAGCAAGGAATCCATCCTGCCCAAGAGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCTAAGAAGTATGGAGGCTTCGACAGCCCCACCGTAGCCTACAGCGTGCTGGTGGTCGCGAAGGTAGAGAAGGGGAAGAGCAAGAAACTGAAGAGCGTGAAGGCCGAAATCGTTCCTTAGGTAGGACGGGTTCTCCTTGTCGCTGTTCGACTAGCGGGCCTTCTTCCTGACCCTGGGATTCTTCATACCTCCGAAGCTGTCGGGGTGGCATCGGATGTCGCACGACCACCAGCGCTTCCATCTCTTCCCCTTCTCGTTCTTTGACTTCTCGCACTTCCBarIKflINruIR1146L*1148QG1150IH1152PA1154QE1156EQ1158RQ1160AD1162RP1164EE1166GL1168GP1170*E1172VW1174RL1176RQ1178PH1180RS1182LQ1184RA1186GG1188RE1190GR1192EG1194EE1196QE1198TE1200ER1202EG12043215-8231Cas96,6606,6806,7006,7206,7406,7606,7806,8006,820
    AGCTGCTCGGCATAACCATCATGGAGAGGTCCAGCTTTGAGAAGAACCCCATTGACTTTTTGGAAGCCAAGGGCTACAAAGAGGTCAAAAAGGACCTGATCATCAAACTCCCCAAGTACTCCCTGTTTGAATTGGAGAACGGCAGAAAGAGGATGCTGGCGAGCGCTGGGGAACTTCGACGAGCCGTATTGGTAGTACCTCTCCAGGTCGAAACTCTTCTTGGGGTAACTGAAAAACCTTCGGTTCCCGATGTTTCTCCAGTTTTTCCTGGACTAGTAGTTTGAGGGGTTCATGAGGGACAAACTTAACCTCTTGCCGTCTTTCTCCTACGACCGCTCGCGACCCCTTGAAfeIXcmIAA1206RH1208NH1210HG1212EV1214QL1216*E1218EP1220H*1222LF1224GS1226QG1228LQ1230RG1232QK1234GP1236DH1238QT1240PQ1242VL1244PV1246*I1248GE1250RQ1252KE1254DA1256GE1258RW1260GT12623215-8231Cas96,8406,8606,8806,9006,9206,9406,9606,9807,000
    GCAAAAGGGCAACGAACTGGCGCTGCCCAGCAAGTACGTGAATTTTCTGTACCTGGCGTCCCACTACGAAAAGCTGAAAGGCAGCCCCGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCATTACCTGGACGAGATAATCGAGCAAATCAGCGAGTTCAGCAAGCGTTTTCCCGTTGCTTGACCGCGACGGGTCGTTCATGCACTTAAAAGACATGGACCGCAGGGTGATGCTTTTCGACTTTCCGTCGGGGCTCCTGTTGCTCGTCTTCGTCGACAAGCACCTCGTCGTGTTCGTAATGGACCTGCTCTATTAGCTCGTTTAGTCGCTCAAGTCGTTCAK1264GQ1266RT1268GA1270AQ1272QV1274RE1276FS1278VP1280GV1282PL1284RK1286AE1288RQ1290PR1292GQ1294RA1296EA1298AV1300RG1302AA1304QA1306LP1308GR1310DN1312RA1314NQ1316RV1318QQ13203215-8231Cas97,0207,0407,0607,0807,1007,1207,1407,160
    AGGGTGATTCTGGCCGACGCGAACCTGGATAAGGTCCTCAGCGCCTACAACAAGCACCGAGACAAACCCATCAGGGAGCAGGCCGAGAATATCATACACCTGTTCACCCTGACAAATCTGGGCGCACCTGCGGCATTCAAATACTTCGATACCACCATCGACAGGAAAAGGTACATCCCACTAAGACCGGCTGCGCTTGGACCTATTCCAGGAGTCGCGGATGTTGTTCGTGGCTCTGTTTGGGTAGTCCCTCGTCCGGCTCTTATAGTATGTGGACAAGTGGGACTGTTTAGACCCGCGTGGACGCCGTAAGTTTATGAAGCTATGGTGGTAGCTGTCCTTTTCCATGTBbvCIPaqCIEG1322DS1324GR1326RE1328PG1330*G1332PQ1334RL1336QQ1338AP1340RQ1342TH1344QG1346AG1348RE1350YH1352TP1354VH1356PD1358KS1360GR1362TC1364GI1366QI1368LR1370YH1372HR1374QE1376KV1378H3215-8231Cas97,1807,2007,2207,2407,2607,2807,3007,3207,340
    CTAGCACTAAGGAGGTGCTGGATGCCACCTTGATCCACCAGTCCATTACCGGCCTGTATGAGACCAGGATCGACCTGAGCCAGCTTGGAGGCGACTCTAGGGCGGACCCAAAAAAGAAAAGGAAGGTGGAATTCTCTAGAGGCAGTGGAGAGGGCAGAGGAAGTCTGCTAACATGGATCGTGATTCCTCCACGACCTACGGTGGAACTAGGTGGTCAGGTAATGGCCGGACATACTCTGGTCCTAGCTGGACTCGGTCGAACCTCCGCTGAGATCCCGCCTGGGTTTTTTCTTTTCCTTCCACCTTAAGAGATCTCCGTCACCTCTCCCGTCTCCTTCAGACGATTGTACXbaIEcoRI*1380H*1382GG1384AG1386CH1388LD1390PP1392VH1394YR1396PV1398*D1400QD1402RP1404EP1406AW1408RR1410L*1412GG1414PK1416KE1418KE1420GG1422IL1424*R1426QW1428RG1430QR1432KS1434AN1436M3215-8231Cas9T2ASV40 NLS7,3607,3807,4007,4207,4407,4607,4807,5007,520
    CGGTGACGTCGAGGAGAATCCTGGCCCAATGAACCTGAGCAAAAACGTGAGCGTGAGCGTGTATATGAAGGGGAACGTCAACAATCATGAGTTTGAGTACGACGGGGAAGGTGGTGGTGATCCTTATACAGGTAAATATTCCATGAAGATGACGCTACGTGGTCAAAATTCCCTAGCCACTGCAGCTCCTCTTAGGACCGGGTTACTTGGACTCGTTTTTGCACTCGCACTCGCACATATACTTCCCCTTGCAGTTGTTAGTACTCAAACTCATGCTGCCCCTTCCACCACCACTAGGAATATGTCCATTTATAAGGTACTTCTACTGCGATGCACCAGTTTTAAGGGATR1438*R1440RG1442ES1444WP1446NE1448PE1450QK1452RE1454RE1456RV1458YE1460GE1462RQ1464QS1466*V1468*V1470RR1472GR1474WW1476*S1478LY1480R*1482IF1484HE1486DD1488AT1490WS1492KF1494P3215-8231OFPT2A7,5407,5607,5807,6007,6207,6407,6607,6807,700
    CCCTTTTCCTATGATATCATTACCACGGCATTTCAGTATGGTTTCCGCGTATTTACAAAATACCCTGAGGGAATTGTTGACTATTTTAAGGACTCGCTTCCCGACGCATTCCAGTGGAACAGACGAATTGTGTTTGAAGATGGTGGAGTACTAAACATGAGCAGTGATATCACATGGGAAAAGGATACTATAGTAATGGTGCCGTAAAGTCATACCAAAGGCGCATAAATGTTTTATGGGACTCCCTTAACAACTGATAAAATTCCTGAGCGAAGGGCTGCGTAAGGTCACCTTGTCTGCTTAACACAAACTTCTACCACCTCATGATTTGTACTCGTCACTATAGTGTABsu36IT1496LF1498L*1500YH1502YH1504GI1506SV1508WF1510PR1512IY1514KI1516P*1518GN1520C*1522LF1524*G1526LA1528SR1530RI1532PV1534EQ1536TN1538CV1540*R1542WW1544ST1546KH1548EQ1550*Y1552HI15543215-8231OFP7,7207,7407,7607,7807,8007,8207,8407,860
    ATAAAGATAATGTTCTGCATGGTGACGTCAAGGCTGAGGGAGTGAACTTCCCGCCGAATGGGCCAGTGATGAAGAATGAAATTGTGATGGAGGAACCGACTGAAGAAACATTTACTCCAAAAAACGGGGTTCTTGTTGGCTTTTGTCCCAAAGCGTACTTACTTAAAGACGGTTCTATTTCTATTACAAGACGTACCACTGCAGTTCCGACTCCCTCACTTGAAGGGCGGCTTACCCGGTCACTACTTCTTACTTTAACACTACCTCCTTGGCTGACTTCTTTGTAAATGAGGTTTTTTGCCCCAAGAACAACCGAAAACAGGGTTTCGCATGAATGAATTTCTGCCAAGBbvCI*R1556*C1558SA1560W*1562RQ1564G*1566GS1568EL1570PA1572EW1574AS1576DE1578E*1580NC1582DG1584GT1586D*1588RN1590IY1592SK1594KR1596GS1598CW1600LL1602SQ1604SV1606LT1608*R1610RF16123215-8231OFP7,8807,9007,9207,9407,9607,9808,0008,0208,040
    CTATTACTATGGAAATATGACAACATTTTACAGATCCAAGAAATCTGGCCAGGCACCTCCTGGGTATCACTTTGTTAAGCATCGTCTCGTCAAGACCAATGTGGGACATGGATTTAAGACGGTTGAGCAGACTGAATATGCCACTGCTCATGTCAGTGATCTTCCCAAGTTCGAAGATAATGATACCTTTATACTGTTGTAAAATGTCTAGGTTCTTTAGACCGGTCCGTGGAGGACCCATAGTGAAACAATTCGTAGCAGAGCAGTTCTGGTTACACCCTGTACCTAAATTCTGCCAACTCGTCTGACTTATACGGTGACGAGTACAGTCACTAGAAGGGTTCAAGCTTXcmIBstBIHindIIILL1614LW1616KY1618DN1620IL1622QI1624QE1626IW1628PG1630TS1632WV1634SL1636C*1638AS1640SR1642QD1644QC1646GT1648WI1650*D1652G*1654AD1656*I1658CH1660CS1662CQ1664*S1666SQ1668VR16703215-8231OFP8,0608,0808,1008,1208,1408,1608,1808,2008,220
    GCTTGATAATGAGTTTAAACGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCCGAACTATTACTCAAATTTGCCCCCTCCGATTGACTTTGTGCCTTCCTCTGTTATGGCCTTCCTTGGGCGCGATACTGCCGTTATTTTTCTGTCTTATTTTGCGTGCCCACAACCCAGCAAACAAGTATTTGCGCCCCAAGCCAGGGTCCCGACCGTGAGACAGCTATGGGGTGGPasIPmeISL1672HSV TK poly(A) signal8,2408,2608,2808,3008,3208,3408,3608,3808,400
    GAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCAGATCTGCGCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTACTCTGGGGTAACCCCGGTTATGCGGGCGCAAAGAAGGAAAAGGGGTGGGGTGGGGGGTTCAAGCCCACTTCCGGGTCCCGAGCGTCGGTTGCAGCCCCGCCGTCCGGGACGGTATCGTCTAGACGCGTCGACCCCGAGATCCCCCATAGGGGTGCGCGGGACATCGCCGCGTAATFspIPasIf1 ori8,4208,4408,4608,4808,5008,5208,5408,560
    AGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCf1 ori8,5808,6008,6208,6408,6608,6808,7008,7208,740
    ACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGPsiIf1 ori8,7608,7808,8008,8208,8408,8608,8808,9008,920
    GATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTAAGGTCGGGCAGGAAGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTAGAATTAATTTGACTGTAAACACAAAGATATTAGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAATTAATTCCAGCCCGTCCTTCTCCCGGATAAAGGGTACTAAGGAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAATCTTAATTAAACTGACATTTGTGTTTCTATAATCPacIU6 promoterf1 ori8,9408,9608,9809,0009,0209,0409,0609,0809,100
    TACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCGATGTTTTATGCACTGCATCTTTCATTATTAAAGAACCCATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAACTTTCATAAAGCTAAAGAACCGAAATATATAGAACACCTTTCCTGCTTTGTGGCNdeIU6 promoter9,1209,1409,1609,1809,2009,2209,240
    • Linear Map
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    • Description
    Editing disabled on read-only sequence.
    GeneArt® CRISPR Nuclease Vector - OFP (9250 bp)AclIAclIAfeIAhdIAjuIAjuIAleIApaIBarIBarIBbsIBlpIBlpIFseIFspIFspIKflIKpnIMfeIMluINcoINcoINdeINdeINruINruIPacIPasIPasIPciIPciIPmeIPmlIPsiIPvuISacISacISpeISphISphITliIXbaIXcmIXcmIXhoIBamHIBbvCIBbvCIBmgBIBspEIBspEIBsrDIBsrDIBsrGIBsrGIBstBIBstXIBstXICspCIEcoRIPaqCIPaqCIPflFIPflMIPflMIPspXISexAISgrDISnaBIAcc65IBssHIIBstEIIBsu36IBsu36IPaeR7IPspOMIBstZ17IEco53kIEco53kIHindIIITth111I1,0002,0003,0004,0005,0006,0007,0008,0009,0003215-8231Cas9AmpROFPoriGeneArt® CRISPR Nuclease Vector - OFP (9250 bp)

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    9250