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pBT100.106b APOBEC1-intein SapI Golden Gate donor
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    aatgctcttctagtgtacacactaccacgttaaggaggtaaagtatgtcttctgaaaccggtccggttgcggttgacccgaccctgcgtcgtcgtatcgaaccgcacgaattcgaagttttcttcgacccgcgtgaactgcgtaaagaaacctgcctgctgtacgaaatcaactgttacgagaagatcacatgtgtgatggtgcaattcctccatttcatacagaagactttggccaggccaacgccaactgggctgggacgcagcagcatagcttggcgtgcttaagcttcaaaagaagctgggcgcacttgacgcatttctttggacggacgacatgctttagttgacAgeIAhdIBbsISapITatIBfuAIBsaXIBspMIBspQIBsrGIBstBICspCIEcoRIRsrIIMS2SE4TG6PV8AV10DP12TL14RR16RI18EP20HE22FE24VF26FD28PR30EL32RK34ET36CL38LY40EI42NW4445-731rAPOBEC1synthetic RB...from proIII20406080100120140160
    gggtggtcgtcactctatctggcgtcacacctctcagaAcaccaacaaacacgTtgaagttaacttcatcgaaaaattcaccaccgaacgttacttctgcccgaacacccgttgctctatcacctggttcctgtcttggtcaccgtgcggagaatgctctcgtgcgatcaccgaacccaccagcagtgagatagaccgcagtgtggagagtctTgtggttgtttgtgcAacttcaattgaagtagctttttaagtggtggcttgcaatgaagacgggcttgtgggcaacgagatagtggaccaaggacagaaccagtggcacgcctcttacgagagcacgctagtggcttAclIBmrIHpaIBsaHIBssSIEcoRISexAIAflIIIBssSαIBstEIIGG46RH48SI50WR52HT54SQ56NT58NK60HV62EV64NF66IE68KF70TT72ER74YF76CP78NT80RC82SI84TW86FL88SW90SP92CG94EC96SR98AI100TE10245-731rAPOBEC1180200220240260280300320340
    ttcctgtctcgttacccgcacgttaccctgttcatctacatcgcgcgtctgtaccaccacgcggacccgcgtaaccgtcagggtctgcgtgacctgatctcttctggtgttaccatccagatcatgaccgaacaggaatctggttactgctggcgtaacttcgttaactactctcaaggacagagcaatgggcgtgcaatgggacaagtagatgtagcgcgcagacatggtggtgcgcctgggcgcattggcagtcccagacgcactggactagagaagaccacaatggtaggtctagtactggcttgtccttagaccaatgacgaccgcattgaagcaattgatgagagHpaIBcoDIBsmAIBtgZIFL104SR106YP108HV110TL112FI114YI116AR118LY120HH122AD124PR126NR128QG130LR132DL134IS136SG138VT140IQ142IM144TE146QE148SG150YC152WR154NF156VN158YS16045-731rAPOBEC1360380400420440460480500520
    cgtctaacgaagcgcactggccgcgttacccgcacctgtgggttcgtctgtacgttctggaactgtactgcatcatcTTAGGCTTACCACCTTGTTTAAATATTTTACGCCGCaaacagccgcagctgaccttcttcaccatcgcgctgcagtcttgccactaccagcgtctgccgcagattgcttcgcgtgaccggcgcaatgggcgtggacacccaagcagacatgcaagaccttgacatgacgtagtagAATCCGAATGGTGGAACAAATTTATAAAATGCGGCGtttgtcggcgtcgactggaagaagtggtagcgcgacgtcagaacggtgatggtcgcagacggDraIPstITatIBtgZIPvuIIPS162NE164AH166WP168RY170PH172LW174VR176LY178VL180EL182YC184II186LG188LP190PC192LN194IL196RR198KQ200PQ202LT204FF206TI208AL210QS212CH214YQ216RL218P45-731rAPOBEC1recoded to redu...tion with gIII540560580600620640660680700
    gccgcacatcctgtgggcgaccggtctgaaatccggtagcgaaacaccggggacttcagaatcggccaccccggAgtgcctgTcatacgaaaccgagatactgacagtagaatatggccttctgccaatcgggaagattgtggagaaacggatagaatgcacagtttactctgtccggcgtgtaggacacccgctggccagactttaggccatcgctttgtggcccctgaagtcttagccggtggggccTcacggacAgtatgctttggctctatgactgtcatcttataccggaagacggttagcccttctaacacctctttgcctatcttacgtgtcaaatgagacagAgeIBsaXIBsmFIP220HI222LW224AT226GL228K45-731CL2SY4ET6EI8LT10VE12YG14LL16PI18GK20IV22EK24RI26EC28TV30YS32V777-1085S1G2S3E4T5P6G7T8S9E10S11A12T13P14E15732-776Npu DnaE N-term inteinXTEN linkerrAPOBEC1720740760780800820840860
    gataacaatggtaacatttatactcagccagttgcccagtggcacgaccggggagagcaggaagtattcgaatactgtctggaggatggaagtctcattagggccactaaggaccacaaatttatgacagtcgatggccagatgctgcctatagacgaaatctttgagcgagagtctattgttaccattgtaaatatgagtcggtcaacgggtcaccgtgctggcccctctcgtccttcataagcttatgacagacctcctaccttcagagtaatcccggtgattcctggtgtttaaatactgtcagctaccggtctacgacggatatctgctttagaaactcgctctcaBmrIBpmIMscIXcmIBcoDIBsmAIBstBID34NN36GN38IY40TQ42PV44AQ46WH48DR50GE52QE54VF56EY58CL60ED62GS64LI66RA68TK70DH72KF74MT76VD78GQ80ML82PI84DE86IF88ER90E777-1085Npu DnaE N-term intein8809009209409609801,0001,0201,040
    tggacctcatgcgagttgacaaccttcctaattaaggcggctgaagagcctggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaacctggagtacgctcaactgttggaaggattaattccgccgacttctcggaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagattSapIBspQICspCIEcoNIL92DL94MR96VD98NL100PN102*777-1085Npu DnaE N-term intein1,0601,0801,1001,1201,1401,1601,1801,2001,220
    atacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatgtaagtttatacataggcgagtacttaattaagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccAseIXmnIBceAIBciVIBsrBIPflMI*272FF270ED268LM266LH264FQ262LK260NM258DP256ND254IG252YK250QF248LR246KQ244LS242PS240FE238GL236CN234WL232IA230LD228Q1262-2077aph (KanR)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcaAseINsiIY226RD224AI222GV220RG218VD216IC214GI212LK210GE208DF206IL204ND202LS200FD198GH196TV194VS192DP190SF188PL186LK184HM182EK180WV178QE176VP174WG172NR170ED1681262-2077aph (KanR)1,4201,4401,4601,4801,5001,5201,5401,560
    catcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagccagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcggtctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattAjuIBtsIPvuIAsiSIBtsαIEcoNIBpu10IDF166DS164AD162VL160GN158NM156RS154QA152QA150LR148FV146RD144SN142FP140CN138CV136PI134SH132LR130RL128FV126AL124AD122VI120NE118GS116DP114YE112EL1101262-2077aph (KanR)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaacatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgAvaIBtsINsiISmaIXmaIBsoBIBtsαITspMIVQ108FA106TK104GP102IA100TT98LL96WA94DD92PT90RI88FH86KI84TP82LP80MF78ET76LW74NL72RV70ME68DT66VD64NA62VS60GK58GH56KL54FL521262-2077aph (KanR)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttaagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatClaINruIBanIIBspDIBsp1286IEP50AD48PK46GY44LR42YI40TA38GS36QG34VN32DR30AW28KY26GY24LD22AD20MN18SN16LR14PR12SC10ST8ER6QI4HS2M1262-2077aph (KanR)1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tgtaagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgcacattcgtctgtcaaaataacaagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacgpUC origin high copy2,1202,1402,1602,1802,2002,2202,2402,260
    cggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgagcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctBceAIpUC origin high copy2,2802,3002,3202,3402,3602,3802,4002,4202,440
    taagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaagattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttcApaLIBaeGIBseYIHaeIIBsiHKAIBsp1286IpUC origin high copy2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcDrdIEciIEciIBciVIBssSIBssSαIpUC origin high copy2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctcNspIPciIBsrBIAflIIIpredicted terminator2,8202,8402,8602,8802,9002,9202,940
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    Editing is disabled because sequence edits have been locked.
    2958 bppBT100 backbone2000225025002750250500XTEN linkerrAPOBEC1C>TC93 Zn coordination45-731732-776USER junction 16,SapI,AGT for SapI Golden Gate cloning,+15H61 essential, Zn coordination,conserved residues,BmrI,BsaHI,AflIII,HpaIconserved residues,C96 Zn coordination,AclI,SexAI,BstEII,BssSI,BssSαIEcoRIBcoDIBsmAI,BtgZIHpaITatIrecoded to reduce recombination with gIII,DraI,BtgZI,PvuIIPstIAgeIBceAIpUC origin high copyApaLI,BaeGI,BsiHKAI,Bsp1286I,BseYIHaeIIEciI,BciVI,BssSI,BssSαIDrdI,EciIpredicted terminator,AflIII,PciI,NspI,BsrBI

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    2958