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pAAV-nEF-CoffFon-Arch3.3-EYFP-WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaaggatctgcgatcgctccgggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcctagacgctagcgaggcEagIMluINotIPvuISbfIAsiSIAflIIIL-ITRnEF promoter20406080100120140160
    gtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtcacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttgcccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcaMfeIEcoNInEF promoter180200220240260280300320340
    agtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgcctcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtcgacttcgaagctccccgagcgtagagaggaagtgcgcgggcggcgggatggactccggcggtaggtgcggccaactcagcgcaagacggcggagggcggacaccacggaggacttgacgcaggcggCspCIEcoNIBsu36IHindIIInEF promoter360380400420440460480500520
    gtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacagatccattcaaatttcgagtccagctctggcccggaaacaggccgcgagggaacctcggatggatctgagtcggccgagaggtgcgaaacggactgggacgaacgagttgagatgcagaaacaaagcaaaagacaagacgcggcaatgtctaggttcgacactggccgcggatKasINarISfoIPluTInEF promoter540560580600620640660680700
    cggatccgaagttcctattcttcaaatagtataggaacttcgcagagtagtagctggattgtagctgctattagcaatctaaaacctcttagaagttcctattcttcaaaaggtataggaacttcggcgcgccttacacctcgttctcgtagcagaacttgtacagctcgtccatgcctaggcttcaaggataagaagtttatcatatccttgaagcgtctcatcatcgacctaacatcgacgataatcgttagattttggagaatcttcaaggataagaagttttccatatccttgaagccgcgcggaatgtggagcaagagcatcgtcttgaacatgtcgagcaggtaAscIBamHIBsrGIEYFP Exon 3F3F5ER export720740760780800820840860
    gccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactggtagctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgcggctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagagcaaccccagaaacgagtcccgcctgaccatcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctacccccacaagacgaccatcaccagccgctcgacgtgcgacEYFP Exon 38809009209409609801,0001,0201,040
    ccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgcggcaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacagccggtactatatctgcaacaccgacaacatcaacatgaggtcgaacacggggtcctacaacggcaggaggaacttcagctacgggaagtcgagctacgccaagtggtcccacagcgEYFP Exon 31,0601,0801,1001,1201,1401,1601,1801,2001,220
    cctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgggcgaagcactgcaggccgtagccgaaggtggtcacgagggtgggggagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggacctgcatcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgcccgcttcgtgacgtccggcatcggcttccaccagtgctcccacccEYFP Exon 31,2401,2601,2801,3001,3201,3401,3601,3801,400
    ccagggcacgggcagctgtgggacgacatgacttaaccagttaaccatgttctctgaacatgagaagggctggccactctccagtcgacataacttcgtataggatactttatacgaagttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaatggtcccgtgcccgtcgacaccctgctgtactgaattggtcaattggtacaagagacttgtactcttcccgaccggtgagaggtcagctgtattgaagcatatcctatgaaatatgcttcaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattaHpaISalIHincIIHincIICE intron BPlox2722EYFP...n 31,4201,4401,4601,4801,5001,5201,5401,560
    aacttcgtatagtataccttatacgaagttatactagtcctcctgtactcaccttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcacttgaagcatatcatatggaatatgcttcaatatgatcaggaggacatgagtggaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgggagcgggagcggcctgtgcgacttgaacaccggcaaatgcagcggcaggtcgagctggtcctacccgtgSpeIBstZ17IEYFP Exon 2loxNCE i...nor1,5801,6001,6201,6401,6601,6801,7001,7201,740
    caccccggtgaacagctcctcgcccttgctcacgggaccggggttttcttccacgtctcctgcttgctttaacagagagaagttcgtggctccggagccgggcgcgcccacgttgatgtcgatctggtccagggggatgtactcgccctcgctggtgatcctgctcttgtcggcggtggggccacttgtcgaggagcgggaacgagtgccctggccccaaaagaaggtgcagaggacgaacgaaattgtctctcttcaagcaccgaggcctcggcccgcgcgggtgcaactacagctagaccaggtccccctacatgagcgggagcgaccactaggacgagaacagccgcAscIBmgBIBsaBIBspEITSp2AEYFP Exon 21,7601,7801,8001,8201,8401,8601,8801,9001,920
    gcactgacatcggcaccggcactgggttctggtgcctcggtgtcgcccagaatagcccgggatctcaacaggataaagccaaagccgaccttggcagtcacgtccaacaccataaacagcagagtttcgatgcccaggcccaccacgccagcgccctcagtgcctatgatccacacgtgactgtagccgtggccgtgacccaagaccacggagccacagcgggtcttatcgggccctagagttgtcctatttcggtttcggctggaaccgtcagtgcaggttgtggtatttgtcgtctcaaagctacgggtccgggtggtgcggtcgcgggagtcacggatactaggtgtBmgBIArch Exon 21,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggatagggtaagcggtccacagcaccaagaccagagctgtcagggtgttaaaggtagatgccacctcggggccccgctcctttgcagcagatcgcagggatgtagccagaaaatagagcaccactatcatgcaaattgtagagaacaaccaccaactgtatctggctatggccgtcctatcccattcgccaggtgtcgtggttctggtctcgacagtcccacaatttccatctacggtggagccccggggcgaggaaacgtcgtctagcgtccctacatcggtcttttatctcgtggtgatagtacgtttaacatctcttgttggtggttgacatagaccgataccggcaApaIPspOMIArch Exon 22,1202,1402,1602,1802,2002,2202,2402,260
    gtggctcaaggctccgatgaggccagtgacgatcatcagggcgtccacacccaccagggtgccgatggtcacccgatccaccttagcgagaagggccagatccagcagcagaagtggggtggtaaacagccagtcggcgtacctaaacacatgagaaatagagtcataagcacatcaccgagttccgaggctactccggtcactgctagtagtcccgcaggtgtgggtggtcccacggctaccagtgggctaggtggaatcgctcttcccggtctaggtcgtcgtcttcaccccaccatttgtcggtcagccgcatggatttgtgtactctttatctcagtattcgtgtaXcmIArch Exon 2CMV i...ointCMV i...ptor2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gggtcacatacaggagattgtatataacattaatacaattttataaaaaaaagggggggcacaggtaccataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataaggtatactcccagtgtatgtcctctaacatatattgtaattatgttaaaatattttttttcccccccgtgtccatggtattgaagcatatttcataggatatgcttcaataaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattccatatgaAseIKpnIPsiIXmnIAcc65IBstZ17ICMV intron branch pointlox2722 RevloxN Rev2,4602,4802,5002,5202,5402,5602,5802,6002,620
    atacgaagttatgctagcaaccccgacacttacctggcataatagatatccaacatttcgcccccgacggtcacctcagtaagcccgataccaaagaacatagacagatatgcggcggatgcgattccgggcaccaggatagtcacagcgtaatattcccgggcatccttatcggtatgcttcaatacgatcgttggggctgtgaatggaccgtattatctataggttgtaaagcgggggctgccagtggagtcattcgggctatggtttcttgtatctgtctatacgccgcctacgctaaggcccgtggtcctatcagtgtcgcattataagggcccgtaggaatagccBmtINheIEcoRVArch Exon 1CMV ...norlox...ev2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgactccccatccgcggaccagaaagtagaaggttccaatcagcatcagcagagtgccgatgcccagccacagagtttcaggtctgccgtcacccagcaggtcgtaaccagcctgcagagcgatggggtccatggtggcgagctcgaagttcctatactatttgaagaataggaaactgaggggtaggcgcctggtctttcatcttccaaggttagtcgtagtcgtctcacggctacgggtcggtgtctcaaagtccagacggcagtgggtcgtccagcattggtcggacgtctcgctaccccaggtaccaccgctcgagcttcaaggatatgataaacttcttatccttNcoISacIBfuAIBspMISacIIEco53kIArch Exon 1F3 Rev2,8202,8402,8602,8802,9002,9202,9402,960
    cttcttgccttaacccagaaattatcactgttattctttagaatggtgcaaagagaagttcctataccttttgaagaataggaacttcgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctcctgaagaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttctcttcaaggatatggaaaacttcttatccttgaagcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaClaIBspDIBstBIEcoRIEcoRVHindIIIWPREF5 Rev2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtWPRE3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaacPflMIWPRE3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcBbsIEarIZraIAatIISacIIWPRE3,5203,5403,5603,5803,6003,6203,6403,660
    catcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctcAfeIBplIBplIClaITliIXhoIBglIIBspDICspCIPaeR7IhGH polyAWPRE3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgBbsISphIBbvCIhGH polyA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    atgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaatactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattBlpINsiIBstXIhGH polyA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacEagIKasINarINotIPmlISbfISfoIBsaAIPluTIRsrIIR-ITR4,2204,2404,2604,2804,3004,3204,3404,360
    cggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttccttgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaf1 Origin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcBsaAIDraIIIf1 Origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccaPsiIf1 Origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgc4,9204,9404,9604,9805,0005,0205,0405,060
    cgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataagcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactattZraIAatII5,0805,1005,1205,1405,1605,1805,2005,2205,240
    atgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagEarIAmpR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgScaIXmnIAmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcPvuIAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    gagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaagAseIFspIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgaAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcctaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagAmpRpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgttttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcapUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    agttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtAlwNIpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcapUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori6,8406,8606,8806,9006,9206,9406,960
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    6961 bp500055006000650050010001500EYFP Exon 2ER export

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    6961