Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
(pG3 = pKR487) pRS416-tRNA-HDV-Ade2-gRNA-Qi-gRNA_Tef1-Cas9 with RPL25 intron
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTATTTTTATGTTTTGTATTTGGATTTTAGCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCCTGCCTAGCGAACGGACATTGAATGTGCGCGGAGCATAGAAAATTACTACCTTATTAAACCCTTAAATGAGACACAAATAAATAAAAATACAAAACATAAACCTAAAATCCEN/ARS20406080100120140160
    AAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAATCACAGGATTTTCGTTTCATTTATTTCTTCCATCTTCTCAATGCCTTACTTCTTTTTTTTTATTTGTTTCCAAATTTTTTAAAGTTGTTTTTCGCATGAAATGTATATATAAATAATCTGTTCTTTTCGTCTAATTTATCTATATGTAAGCTAATTGCTATTCATTTTACATTTTAGTGTCCTAAAAGCCEN/ARS180200220240260280300320340
    TGTGTGGTCTTCTACACAGACAAGATGAAACAATTCGGCATTAATACCTGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAACACACCAGAAGATGTGTCTGTTCTACTTTGTTAAGCCGTAATTATGGACTCTCGTCCTTCTCGTTCTATTTTCCATCATAAACAACCGCTAGGGGGATCTCAGAAAATGTAGAAGCCTTTTGTTTTTGATAAAAAAGAAATTAAAGAAAAAAATGAAAGATAAAAATTAAATATCEN/ARS360380400420440460480500520
    TATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATGAAAAGGACCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTATAAATATAATTTTTTAAATTTAATATTAATAAAAATATCGTGCACTACTTTTCCTGGGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAAPmlISwaIAmpR promoterCEN/ARS540560580600620640660680700
    GAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAAmpR open reading frame (ORF)AmpR...ter720740760780800820840860
    CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCAmpR open reading frame (ORF)8809009209409609801,0001,0201,040
    GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCACTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTAmpR open reading frame (ORF)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATANmeAIIIAmpR open reading frame (ORF)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTAhdIAmpR open reading frame (ORF)1,4201,4401,4601,4801,5001,5201,5401,560
    CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCAmpR terminator1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGori2,1202,1402,1602,1802,2002,2202,2402,260
    ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCTTAATTAAGTATATGTGTTATGTAGTATACTCTTTCTTCAACAATTAAATACTCTCGGTAGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGAATTAATTCATATACACAATACATCATATGAGAAAGAAGTTGTTAATTTATGAGAGCCATCPacIoritRNA-Tyr (SUP4)US_gRNA_fwd2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCAAGTTGGTTTAAGGCGCAAGACTGTAATTTATCACTACGAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGAGATGGCCGGCATGGTCCCAGCCTCCTCGCTGGCGCCGGCTGGGCAACACCTTCGGGTGGCGAATGGGACTTTGTATAATGTCCAGAGTTGTGgtttGGTTCAACCAAATTCCGCGTTCTGACATTAAATAGTGATGCTTTAGAACTCTAGCCCGCAAGCTGAGCGGGGGCCCTCTCTACCGGCCGTACCAGGGTCGGAGGAGCGACCGCGGCCGACCCGTTGTGGAAGCCCACCGCTTACCCTGAAACATATTACAGGTCTCAACACcaaaBstXItRNA-Tyr (SUP4)HDV-RibozymeADE2 gRNA 32,4602,4802,5002,5202,5402,5602,5802,6002,620
    aagagctatgctggaaacagcatagcaagtttaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttGTTAACTCCGTTACTGTGGGCTTGCCATATTTTTTGGAACTTTTCTGCCCTTTTTCTCAATGAGTAAGGAGGGCGTTTATCTTTTCTttctcgatacgacctttgtcgtatcgttcaaatttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaCAATTGAGGCAATGACACCCGAACGGTATAAAAAACCTTGAAAAGACGGGAAAAAGAGTTACTCATTCCTCCCGCAAATAGAAAAGAHpaICas9 gRNA scaffoldU5 terminator (SNR7)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ATTTTATTTTTCTACAAATTTGATTCATGAGTCCATGGAACAAATATATAGAACTCATTTTCTTGTTTCGGTATATATATTAACTCCATTGAAGCATTTGCTTTTATCCTGACTGGCTAGCtagtcgtgcaatgtatgactttaagCCGCGGTCTTTCCTGCGTTATCCCCTGATTAAAATAAAAAGATGTTTAAACTAAGTACTCAGGTACCTTGTTTATATATCTTGAGTAAAAGAACAAAGCCATATATATAATTGAGGTAACTTCGTAAACGAAAATAGGACTGACCGATCGatcagcacgttacatactgaaattcGGCGCCAGAAAGGACGCAATAGGGGACTABmtINcoINheISacIIU5 terminator (SNR7)DS_gRNA_rev2,8202,8402,8602,8802,9002,9202,9402,960
    TCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGA2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTcaCACAggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcaCCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAgtGTGTcctttgtcgatactggtactaatgcggttcgcgcgttaattgggagtM13_revT3_...er3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctaaagggaacaaaagctggAGCTCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGgatttcccttgttttcgaccTCGAGTATCGAAGTTTTACAAAGATGAGGAAAAAATGAGAAGGTCTAAAAGAGCCTGAGGCGCGTAGCGGCATGGTGAAGTTTTGTGGGTTCGTGTCGTATGATTTAAAGGGGAGAAAGAAGGAGATCCCACAGCAATTAATGGGCATGATTTCCSacIEco53kITEF Promoter3,3403,3603,3803,4003,4203,4403,4603,4803,500
    TTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTAAACCTTTTCTTTTTTCTCTGGCGGAGCAAAGAAAAAGAAGCAGCTTTTTCCGTTATTTTTAAAAATAGTGCAAAGAAAAAGAACTTTTAAAAAAAAAACTAAAAAAAGAGAAAGCTACTGGAGGGTAACTATAAATTCAATTATTTGCCAGAAGTTAAAGAGTTCAAAGTCAAATEF Promoter3,5203,5403,5603,5803,6003,6203,6403,660
    CATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTCTAGAGatgtctagagccccaaagaagaagagaaaagttagacccgggATGGACAAGAAGTACTCCATTGGGCTCGATATCGGCACAAACAGCGTCGGGTAAAAAGAACAAGATAATGTTGAAAAAAATGAAGAACGAGTAATCTTTCTTTCGTATCGTTAGATTAGATTCAAAAGATCTCtacagatctcggggtttcttcttctcttttcaatctgggcccTACCTGTTCTTCATGAGGTAACCCGAGCTATAGCCGTGTTTGTCGCAGCCXbaIXbaIEcoRVM1S2R3A4P5K6K7K8R9K10V11R12P13G143759-3800MD2KK4YS6IG8LD10IG12TN14SV16G3801-8354Cas9 exon 1TEF Promoternuclear localizati...(NLS) with linker3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CTGGGCCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGTTCTGGGCAATACCGATCGCCACAGCATAAAGAAGAACCTCATTGGCGCCCTCCTGTTCGACTCCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCGCAGAGACCCGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACCCGTTATGGCTAGCGGTGTCGTATTTCTTCTTGGAGTAACCGCGGGAGGACAAGCTGAGGCCCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTNmeAIIIW18AV20IT22DE24YK26VP28SK30KF32KV34LG36NT38DR40HS42IK44KN46LI48GA50LL52FD54SG56ET58AE60AT62RL64KR66TA68RR70RY72TR74R3801-8354Cas9 exon 13,8603,8803,9003,9203,9403,9603,9804,0004,020
    AAGAATCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAGGTGGATGACTCTTTCTTCCATAGGCTGGAGGAGTCCTTTTTGGTGGAGGAGGATAAAAAGCACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTACCCAATTCTTAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGAGAAAGAAGGTATCCGACCTCCTCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGGTGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTSbfIXcmIBglIIPflMIPflMIK76NR78IC80YL82QE84IF86SN88EM90AK92VD94DS96FF98HR100LE102ES104FL106VE108ED110KK112HE114RH116PI118FG120NI122VD124EV126AY128HE130KY132P3801-8354Cas9 exon 14,0404,0604,0804,1004,1204,1404,1604,1804,200
    CCATATATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACTTGCGGTTGATCTATCTCGCGCTGGCGCATATGATCAAATTTCGGGGACACTTCCTCATCGAGGGGGACCTGAACCCAGACAACAGCGATGTCGACAAACTCTTTATCCAACTGGTTCAGACTTAGGTATATAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAGAGCGCGACCGCGTATACTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGGGTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATNdeISalIEcoNIT134IY136HL138RK140KL142VD144ST146DK148AD150LR152LI154YL156AL158AH160MI162KF164RG166HF168LI170EG172DL174NP176DN178SD180VD182KL184FI186QL188VQ190TY1923801-8354Cas9 exon 14,2204,2404,2604,2804,3004,3204,3404,360
    CAATCAGCTTTTCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAAGCAATCCTGAGCGCTAGGCTGTCCAAATCCCGGCGGCTCGAAAACCTCATCGCACAGCTCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCTGACCCCCGTTAGTCGAAAAGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCGACAGGTTTAGGGCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGCCGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGPasIBspEINQ194LF196EE198NP200IN202AS204GV206DA208KA210IL212SA214RL216SK218SR220RL222EN224LI226AQ228LP230GE232KK234NG236LF238GN240LI242AL244SL246GL248TP2503801-8354Cas9 exon 14,3804,4004,4204,4404,4604,4804,5004,5204,540
    AACTTTAAATCTAACTTCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGCAAAGACACCTACGATGATGATCTCGACAATCTGCTGGCCCAGATCGGCGACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAGTGATATTCTGCGAGTGATTGAAATTTAGATTGAAGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGCTGTTAGACGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGGACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTBsaBINF252KS254NF256DL258AE260DA262KL264QL266SK268DT270YD272DD274LD276NL278LA280QI282GD284QY286AD288LF290LA292AK294NL296SD298AI300LL302SD304IL306RV3083801-8354Cas9 exon 14,5604,5804,6004,6204,6404,6604,6804,7004,720
    ACACGGAGATCACCAAAGCTCCGCTGAGCGCTAGTATGATCAAGCGCTATGATGAGCACCACCAAGACTTGACTTTGCTGAAGGCCCTTGTCAGACAGCAACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGGATACATTGACGGCGGAGCTGTGCCTCTAGTGGTTTCGAGGCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGACTTCCGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGATTTTTACCGATGCGGCCTATGTAACTGCCGCCTCGBlpINT310EI312TK314AP316LS318AS320MI322KR324YD326EH328HQ330DL332TL334LK336AL338VR340QQ342LP344EK346YK348EI350FF352DQ354SK356NG358YA360GY362ID364GG366A3801-8354Cas9 exon 14,7404,7604,7804,8004,8204,8404,8604,8804,900
    AAGCCAGGAGGAATTTTACAAATTTATTAAGCCCATCTTGGAAAAAATGGACGGCACCGAGGAGCTGCTGGTAAAGCTTAACAGAGAAGATCTGTTGCGCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACTGCACGCTATCCTCAGGCGGTTCGGTCCTCCTTAAAATGTTTAAATAATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGTCTCTTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGGACCCGCTTGACGTGCGATAGGAGTCCGCCBglIIBsu36IS368QE370EF372YK374FI376KP378IL380EK382MD384GT386EE388LL390VK392LN394RE396DL398LR400KQ402RT404FD406NG408SI410PH412QI414HL416GE418LH420AI422LR424R3801-8354Cas9 exon 14,9204,9404,9604,9805,0005,0205,0405,060
    CAAGAGGATTTCTACCCCTTTTTGAAAGATAACAGGGAAAAGATTGAGAAAATCCTCACATTTCGGATACCCTACTATGTAGGCCCCCTCGCCCGGGGAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAACTTCGAGGAAGTCGTGGATAGTTCTCCTAAAGATGGGGAAAAACTTTCTATTGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCGGGGGAGCGGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGACCTTGAAGCTCCTTCAGCACCTATQ426ED428FY430PF432LK434DN436RE438KI440EK442IL444TF446RI448PY450YV452GP454LA456RG458NS460RF462AW464MT466RK468SE470ET472IT474PW476NF478EE480VV482D3801-8354Cas9 exon 15,0805,1005,1205,1405,1605,1805,2005,2205,240
    AGGGGGCCTCTGCCCAGTCCTTCATCGAAAGGATGACTAACTTTGATAAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGCTGTACGAGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAAAGCCAGCATTCCTGTCTGGTCCCCCGGAGACGGGTCAGGAAGTAGCTTTCCTACTGATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGACGACATGCTCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTTTCGGTCGTAAGGACAGACCBarISacIBsaBIEco53kIK484GA486SA488QS490FI492ER494MT496NF498DK500NL502PN504EK506VL508PK510HS512LL514YE516YF518TV520YN522EL524TK526VK528YV530TE532GM534RK536PA538FL540SG5423801-8354Cas9 exon 15,2605,2805,3005,3205,3405,3605,3805,4005,420
    AGAGCAGAAGAAAGCTATCGTGGACCTCCTCTTCAAGACGAACCGGAAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATTGAATGTTTCGACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACGATCTCCTGAAAATCTCTCGTCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCTTGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTAACTTACAAAGCTGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGCTAGAGGACTTTTAGPasIEQ544KK546AI548VD550LL552FK554TN556RK558VT560VK562QL564KE566DY568FK570KI572EC574FD576SV578EI580SG582VE584DR586FN588AS590LG592TY594HD596LL598KI6003801-8354Cas9 exon 15,4405,4605,4805,5005,5205,5405,5605,5805,600
    ATTAAAGACAAGGACTTCCTGGACAATGAGGAGAACGAGGACATTCTTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGATGATTGAAGAACGCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCGCCGATATACAGGATTAATTTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAGAACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCTACTAACTTCTTGCGAACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTATATGTCCTAPflFIPflFITth111ITth111IIK602DK604DF606LD608NE610EN612ED614IL616ED618IV620LT622LT624LF626ED628RE630MI632EE634RL636KT638YA640HL642FD644DK646VM648KQ650LK652RR654RY656TG6583801-8354Cas9 exon 15,6205,6405,6605,6805,7005,7205,7405,760
    GGGGGCGGCTGTCAAGAAAACTGATCAATGGGGTATGTGAACTGCAATATTAATAGCACGAGAAAATTGAGAGGAAGATAGATGGGAACTAGTAGAGTTGATATTGATGAGATACGAAAACCACACGTAAATAAACTATCCGACGACAAGAATAGTGCATTAAGGACTTGATTAACCCCCGCCGACAGTTCTTTTGACTAGTTACCCCATACACTTGACGTTATAATTATCGTGCTCTTTTAACTCTCCTTCTATCTACCCTTGATCATCTCAACTATAACTACTCTATGCTTTTGGTGTGCATTTATTTGATAGGCTGCTGTTCTTATCACGTAATTCCTGAACTAATTSpeIWG660RL662SR664KL666IN668GV670CE672LQ674Y*676*H678EK680IE682RK684ID686GN688**690S*692Y*694*D696TK698TT700RK702*T704IR706RQ708E*710CI712KD714LI716K3801-8354RPL25 IntronCas9 exon 15,7805,8005,8205,8405,8605,8805,9005,9205,940
    GATATGGTGAGCAACGTAATTATCGGGCTCAACAGTTTATTAGCAATCGTTTTGATAGAAGCGTTGATGCTGTGGAAGTTGTTCTTTTTACTACCAACAGCCATTAACAAATCCATTAGAGTGTTCGTTCGTTTTTCTGCTCAGATTCAAGAAAATTATTCCATCCTCATTATACCTATACCACTCGTTGCATTAATAGCCCGAGTTGTCAAATAATCGTTAGCAAAACTATCTTCGCAACTACGACACCTTCAACAAGAAAAATGATGGTTGTCGGTAATTGTTTAGGTAATCTCACAAGCAAGCAAAAAGACGAGTCTAAGTTCTTTTAATAAGGTAGGAGTAATATGI718W*720AT722*L724SG726ST728VY730*Q732SF734**736KR738*C740CG742SC744SF746YY748QQ750PL752TN754PL756EC758SF760VF762LL764RF766KK768II770PS772SL774Y3801-8354RPL25 Intron5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TTTTTTCTTCTATTTCGTGCTCCACGTCGAGGTATCAAGGAACATAGTTTACTAACATTAACGAATTCATCTCCTATGAATTTACTTTTTGTATAGATCCGAGACAAGCAGAGTGGAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGCCAACCGGAACTTCATGCAGTAAAAAAGAAGATAAAGCACGAGGTGCAGCTCCATAGTTCCTTGTATCAAATGATTGTAATTGCTTAAGTAGAGGATACTTAAATGAAAAACATATCTAGGCTCTGTTCGTCTCACCTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACGGTTGGCCTTGAAGTACGTCAPsrIAflIIBmgBICspCIEcoRIF776FL778LF780RA782PR784RG786IK788EH790SL792LT794LT796NS798SP800MN802LL804FV806*I808RD810KQ812SG814KT816IL818DF820LK822SD824GF826AN828RN830FM832Q3801-8354RPL25 IntronCas9 exon 26,1406,1606,1806,2006,2206,2406,2606,2806,300
    TGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACAAGTTTCTGGCCAGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCAGCTATCAAAAAGGGAATACTGCAGACCGTTAAGGTCGTGGATGAACTCGTCAAAGTAATGGGAAGGCAACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGGTCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTTTTTCCCTTATGACGTCTGGCAATTCCAGCACCTACTTGAGCAGTTTCATTACCCTTCCGTAjuIL834IH836DD838SL840TF842KE844DI846QK848AQ850VS852GQ854GD856SL858HE860HI862AN864LA866GS868PA870IK872KG874IL876QT878VK880VV882DE884LV886KV888MG890RH8923801-8354Cas9 exon 26,3206,3406,3606,3806,4006,4206,4406,460
    TAAGCCCGAGAATATCGTTATCGAGATGGCCCGAGAGAACCAAACTACCCAGAAGGGACAGAAGAACAGTAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAAGAACTGGGGTCCCAAATCCTTAAGGAACACCCAGTTGAAAACACCCAGCTTCAGAATGAGAAGCTCTACATTCGGGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCTTGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCCAGGGTTTAGGAATTCCTTGTGGGTCAACTTTTGTGGGTCGAAGTCTTACTCTTCGAGATGKflIAflIIKP894EN896IV898IE900MA902RE904NQ906TT908QK910GQ912KN914SR916ER918MK920RI922EE924GI926KE928LG930SQ932IL934KE936HP938VE940NT942QL944QN946EK948LY9503801-8354Cas9 exon 26,4806,5006,5206,5406,5606,5806,6006,6206,640
    CTGTACTACCTGCAGAACGGCAGGGACATGTACGTGGATCAGGAACTGGACATCAATCGGCTCTCCGACTACGACGTGGATCATATCGTGCCCCAGTCTTTTCTCAAAGATGATTCTATTGATAATAAAGTGTTGACAAGATCCGATAAAAATAGAGGGAAGAGTGATAACGTCCGACATGATGGACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGATGCTGCACCTAGTATAGCACGGGGTCAGAAAAGAGTTTCTACTAAGATAACTATTATTTCACAACTGTTCTAGGCTATTTTTATCTCCCTTCTCACTATTGCAGGPciIBmgBILY952YL954QN956GR958DM960YV962DQ964EL966DI968NR970LS972DY974DV976DH978IV980PQ982SF984LK986DD988SI990DN992KV994LT996RS998DK1000NR1002GK1004SD1006NV10083801-8354Cas9 exon 26,6606,6806,7006,7206,7406,7606,7806,8006,820
    CCTCAGAAGAAGTTGTCAAGAAAATGAAAAATTATTGGCGGCAGCTGCTGAACGCCAAACTGATCACACAACGGAAGTTCGATAATCTGACTAAGGCTGAACGAGGTGGCCTGTCTGAGTTGGATAAAGCCGGCTTCATCAAAAGGCAGCTTGTTGAGACACGCCAGATCACCAAGGAGTCTTCTTCAACAGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCTTCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGACTCAACCTATTTCGGCCGAAGTAGTTTTCCGTCGAACAACTCTGTGCGGTCTAGTGGTTPS1010EE1012VV1014KK1016MK1018NY1020WR1022QL1024LN1026AK1028LI1030TQ1032RK1034FD1036NL1038TK1040AE1042RG1044GL1046SE1048LD1050KA1052GF1054IK1056RQ1058LV1060ET1062RQ1064IT1066K3801-8354Cas9 exon 26,8406,8606,8806,9006,9206,9406,9606,9807,000
    GCACGTGGCCCAAATTCTCGATTCACGCATGAACACCAAGTACGATGAAAATGACAAACTGATTCGAGAGGTGAAAGTTATTACTCTGAAGTCTAAGCTGGTCTCAGATTTCAGAAAGGACTTTCAGTTTTATAAGGTGAGAGAGATCAACAATTACCACCATGCGCATGATGCCCGTGCACCGGGTTTAAGAGCTAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAATAATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCCTGAAAGTCAAAATATTCCACTCTCTCTAGTTGTTAATGGTGGTACGCGTACTACGGPmlIPsrIBstXIFspAIH1068VA1070QI1072LD1074SR1076MN1078TK1080YD1082EN1084DK1086LI1088RE1090VK1092VI1094TL1096KS1098KL1100VS1102DF1104RK1106DF1108QF1110YK1112VR1114EI1116NN1118YH1120HA1122HD1124A3801-8354Cas9 exon 27,0207,0407,0607,0807,1007,1207,1407,160
    TACCTGAATGCAGTGGTAGGCACTGCACTTATCAAAAAATATCCCAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGTACGATGTTAGGAAAATGATCGCAAAGTCTGAGCAGGAAATAGGCAAGGCCACCGCTAAGTACTTCTTTTACAGCAATATTATGAATTTTTATGGACTTACGTCACCATCCGTGACGTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACATGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCTTTATCCGTTCCGGTGGCGATTCATGAAGAAAATGTCGTTATAATACTTAAAAAY1126LN1128AV1130VG1132TA1134LI1136KK1138YP1140KL1142ES1144EF1146VY1148GD1150YK1152VY1154DV1156RK1158MI1160AK1162SE1164QE1166IG1168KA1170TA1172KY1174FF1176YS1178NI1180MN1182F3801-8354Cas9 exon 27,1807,2007,2207,2407,2607,2807,3007,3207,340
    TCAAGACCGAGATTACACTGGCCAATGGAGAGATTCGGAAGCGACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACAAGGGTAGGGATTTCGCGACAGTCCGGAAGGTCCTGTCCATGCCGCAGGTGAACATCGTTAAAAAGACCGAAGTACAGACCGGAGGAGTTCTGGCTCTAATGTGACCGGTTACCTCTCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGTTCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCAATTTTTCTGGCTTCATGTCTGGCCTCCNruIBspEIPaqCIF1184KT1186EI1188TL1190AN1192GE1194IR1196KR1198PL1200IE1202TN1204GE1206TG1208EI1210VW1212DK1214GR1216DF1218AT1220VR1222KV1224LS1226MP1228QV1230NI1232VK1234KT1236EV1238QT1240GG12423801-8354Cas9 exon 27,3607,3807,4007,4207,4407,4607,4807,5007,520
    CTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAACAGCGACAAGCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTCGATTCTCCTACAGTCGCTTACAGTGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAGTCTAAAAAACTCAAAAGCGTCAAGGAAGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGTCGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAAGAGGATGTCAGCGAATGTCACATGACCAACACCGGTTTCACCTCTTTCCCTTCAGATTTTTTGAGTTTTCGCAGTTCCTTKflIFS1244KE1246SI1248LP1250KR1252NS1254DK1256LI1258AR1260KK1262DW1264DP1266KK1268YG1270GF1272DS1274PT1276VA1278YS1280VL1282VV1284AK1286VE1288KG1290KS1292KK1294LK1296SV1298KE13003801-8354Cas9 exon 27,5407,5607,5807,6007,6207,6407,6607,6807,700
    CTGCTGGGCATCACAATCATGGAGCGATCAAGCTTCGAAAAAAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAAGACCTCATCATTAAGCTTCCCAAGTACTCTCTCTTTGAGCTTGAAAACGGCCGGAAACGAATGCTCGCTAGTGCGGGCGAGCTGCGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTTTTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGTAATTCGAAGGGTTCATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTGCTTACGAGCGATCACGCCCGCTCGACGTliIXhoIPaeR7ILL1302GI1304TI1306ME1308RS1310SF1312EK1314NP1316ID1318FL1320EA1322KG1324YK1326EV1328KK1330DL1332II1334KL1336PK1338YS1340LF1342EL1344EN1346GR1348KR1350ML1352AS1354AG1356EL13583801-8354Cas9 exon 27,7207,7407,7607,7807,8007,8207,8407,860
    AGAAAGGTAACGAGCTGGCACTGCCCTCTAAATACGTTAATTTCTTGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAAGATAATGAGCAGAAGCAGCTGTTCGTGGAACAACACAAACACTACCTTGATGAGATCATCGAGCAAATAAGCGAATTCTCCAAAAGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGAACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCGTCGACAAGCACCTTGTTGTGTTTGTGATGGAACTACTCTAGTAGCTCGTTTATTCGCTTAAGAGGTTTTCEcoRIQK1360GN1362EL1364AL1366PS1368KY1370VN1372FL1374YL1376AS1378HY1380EK1382LK1384GS1386PE1388DN1390EQ1392KQ1394LF1396VE1398QH1400KH1402YL1404DE1406II1408EQ1410IS1412EF1414SK1416R3801-8354Cas9 exon 27,8807,9007,9207,9407,9607,9808,0008,0208,040
    AGTGATCCTCGCCGACGCTAACCTCGATAAGGTGCTTTCTGCTTACAATAAGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAACATTATCCACTTGTTTACTCTGACCAACTTGGGCGCGCCTGCAGCCTTCAAGTACTTCGACACCACCATAGACAGAAAGCGGTACACCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTATTCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACTGGTTGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTGTGGTGGTATCTGTCTTTCGCCATGTGGAscIEcoNIV1418IL1420AD1422AN1424LD1426KV1428LS1430AY1432NK1434HR1436DK1438PI1440RE1442QA1444EN1446II1448HL1450FT1452LT1454NL1456GA1458PA1460AF1462KY1464FD1466TT1468ID1470RK1472RY1474T3801-8354Cas9 exon 28,0608,0808,1008,1208,1408,1608,1808,2008,220
    TCTACAAAGGAGGTCCTGGACGCCACACTGATTCATCAGTCAATTACGGGGCTCTATGAAACAAGAATCGACCTCTCTCAGCTCGGTGGAGACAGCAGGGCTGACCCCAAGAAGAAGAGGAAGGTGTGATCTCTTCTCGAGTCATGTAATTAGTTATGTCACGCTTACATTCACGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGATACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGGGGTTCTTCTTCTCCTTCCACACTAGAGAAGAGCTCAGTACATTAATCAATACAGTGCGAATGTAAGTGCTliIXhoIPaeR7IS1476TK1478EV1480LD1482AT1484LI1486HQ1488SI1490TG1492LY1494ET1496RI1498DL1500SQ1502LG1504GD1506SR1508AD1510PK1512KK1514RK1516V*15183801-8354Cas9 exon 2CYC1 Terminator8,2408,2608,2808,3008,3208,3408,3608,3808,400
    CCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGGGGAGGGGGGTGTAGGCGAGATTGGCTTTTCCTTCCTCAATCTGTTGGACTTCAGATCCAGGGATAAATAAAAAAATATCAATACAATCATAATTCTTGCAATAAATATAAAGTTTAAAAAGAAAAAAAAGACATGTCTGCGCACATGCGTACATTGTAATATGACTTTTGGAACMluIBsrGICYC1 Terminator8,4208,4408,4608,4808,5008,5208,5408,560
    CTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCGGCCGGTACccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGAACTCTTCCAAAACCCTGCGAGCTTCCGAAATTAAACGCCGGCCATGggttaagcgggatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgtTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACKpnIAcc65IlacZαCYC1 TerminatorT7T7_promoterM13-fwd8,5808,6008,6208,6408,6608,6808,7008,7208,740
    GCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGlacZαf1 ori8,7608,7808,8008,8208,8408,8608,8808,9008,920
    CCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCf1 orilacZα8,9408,9608,9809,0009,0209,0409,0609,0809,100
    AGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTAAloIf1 ori9,1209,1409,1609,1809,2009,2209,2409,260
    TTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAGGGTAATAACTGATATAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTTTAGTTTTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCCTAAGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATCCCATTATTGACTATATTAATTTAACTTCGAGATTAAACACTCAAATCATATGTACGTAAATGAATATTATGTCAAAAAATCAAAACGACCGGCGTAGAAGAGTTTATACGAAGGGTCGGANsiI*268NQ266QG264CR262RL260YA258EW256G9408-10211URA3 terminatorURA39,2809,3009,3209,3409,3609,3809,4009,4209,440
    GCTTTTCTGTAACGTTCACCCTCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAATAATAATGTCAGATCCTGTAGAGACCACATCATCCACGGTTCTATACTGTTGACCCAATGCGTCTCCCTTGTCATCTAAACCCACACCGGGTGTCATAATCAACCAATCGAAAAGACATTGCAAGTGGGAGATGGAATCGTAGGGAAGGGAAACGTTTATCAGGAGAAGGTTGTTATTATTACAGTCTAGGACATCTCTGGTGTAGTAGGTGCCAAGATATGACAACTGGGTTACGCAGAGGGAACAGTAGATTTGGGTGTGGCCCACAGTATTAGTTGGTTAA254KR252YR250EG248EV246KA244DR242GK240AF238LG236RG234VI232II230DS228GT226SV224VD222DV220TR218YQ216QG214LA212DG210KD208DL206GV204GP202TM200IL198WD1969408-10211URA39,4609,4809,5009,5209,5409,5609,5809,6009,620
    CGTAACCTTCATCTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAATCTTTGTCGCTCTTCGCAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATGACAATTCTGCTAACATCAAAAGGCCTCTAGGTTCCTTTGTTACTTCTTCGCATTGGAAGTAGAGAAGGTGGGTACAGAGAAACTCGTTATTTCGGCTATTGTTTTAGAAACAGCGAGAAGCGTTACAGTTGTCATGGGAATCATATAAGAGGTCATCTATCCCTCGGGAACGTACTGTTAAGACGATTGTAGTTTTCCGGAGATCCAAGGAAACAATGAAGAAGStuIYG194ED192RG190GM188DR186QA184IF182GI180VF178DK176DS174KA172ID170VT168GK166TY164EG162TS160LS158GK156CS154LE152AL150ML148LG146RP144EK142TV140EE1389408-10211URA39,6409,6609,6809,7009,7209,7409,7609,7809,800
    TGCCGCCTGCTTCAAACCGCTAACAATACCTGGGCCCACCACACCGTGTGCATTCGTAATGTCTGCCCATTCTGCTATTCTGTATACACCCGCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTCTGTCTTCGAAGAGTAAAAAATTGTACTTGGCGGATAATACGGCGGACGAAGTTTGGCGATTGTTATGGACCCGGGTGGTGTGGCACACGTAAGCATTACAGACGGGTAAGACGATAAGACATATGTGGGCGTCTCATGACGTTAAACTGACATAATGGTTACAGTCGTTTAAAAGACAGAAGCTTCTCATTTTTTAACATGAACCGCCTATTAApaIPspOMIAA136QK134LG132SV130IG128PG126VV124GH122AN120TI118DA116WE114AI112RY110VG108AS106YQ104LK102VT100NG98ID96AF94KR92DE90FL88LF86NY84KA82SL809408-10211URA39,8209,8409,8609,8809,9009,9209,9409,960
    GCCTTTAGCGGCTTAACTGTGCCCTCCATGGAAAAATCAGTCAAGATATCCACATGTGTTTTTAGTAAACAAATTTTGGGACCTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACATCCAATGAAGCACACAAGTTTGTTTGCTTTTCGTGCATGATATTAAATACGGAAATCGCCGAATTGACACGGGAGGTACCTTTTTAGTCAGTTCTATAGGTGTACACAAAAATCATTTGTTTAAAACCCTGGATTACGAAGTTGATTGAGGTCATTAAGGAACCACCATGCTTGTAGGTTACTTCGTGTGTTCAAACAAACGAAAAGCACGTACTATAATTTATNcoIPciIXcmIEcoRVAK78LP76KV74TG72EM70SF68DT66LI64DV62HT60KL58LC56IK54PG52LA50EV48LE46LL44EK42TT40RV38DL36SA34CL32NT30QK28EH26MI24NF22L9408-10211URA39,98010,00010,02010,04010,06010,08010,10010,12010,140
    GCTTGGCAGCAACAGGACTAGGATGAGTAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTCGTTTCCTGCAGGTTTTTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGTTTCTTCAACACTACATATGCGTATATATACCAATCTAAGTCTGTGCGAACCGTCGTTGTCCTGATCCTACTCATCGTCGTGCAAGGAATATACATCGAAAGCTGTACTAAATAGAAGCAAAGGACGTCCAAAAACAAGACACGTCAACCCAATTCTTATGACCCGTTAAAGTACAAAGAAGTTGTGATGTATACGCATATATATGGTTAGATTCAGACACNdeISbfIK20AA18VP16SP14HT12AA10RE8KY6TA4KS2M9408-10211URA3 promoterURA310,16010,18010,20010,22010,24010,26010,28010,30010,320
    CTCCTTCCTTCGTTCTTCCTTCTGTTCGGAGATTACCGAATCAAAAAAATTTCAAAGAAACCGAAATCAAAAAAAAGAATAAAAAAAAAATGATGAATTGAATTGAAAAGCTGTGGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACGAGGAAGGAAGCAAGAAGGAAGACAAGCCTCTAATGGCTTAGTTTTTTTAAAGTTTCTTTGGCTTTAGTTTTTTTTCTTATTTTTTTTTTACTACTTAACTTAACTTTTCGACACCATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGGGCTGTGURA3 promoter10,34010,36010,38010,40010,42010,44010,46010,48010,500
    CCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCT10,52010,54010,56010,58010,60010,620
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10627 bp75008000850090009500100005001000150020002500US_gRNA_fwdf1 ori3801-83549408-10211CEN/ARS,SwaI,PmlIAmpR promoterAmpR open reading frame (ORF)NmeAIII,AhdIAmpR terminatororitRNA-Tyr (SUP4),PacIHDV-Ribozyme,ADE2 gRNA 3,BstXICas9 gRNA scaffoldEcoRI,EcoNIAscICYC1 Terminator,PaeR7I,TliI,XhoIT7,T7_promoter,M13-fwd,BsrGI,MluI,Acc65I,KpnIlacZαAloIURA3 terminator,NsiIURA3,StuI,PspOMI,ApaI,NcoI,EcoRVURA3 promoter,PciI,XcmI,SbfI,NdeI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    10627