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XZ087 SFFV-mCherry-sensor(tight)-hIgG1-Fc
  • Sequence Map
    Editing disabled on read-only sequence.
    agtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaagctagctgctcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaataccgttcgatcgacgBmtIMfeINheISFFV20406080100120140160
    agtaacgccattattgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccgcagtttcggccccggctcattgcggtaataacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggcgtcaaagccggggccgEcoRVSFFV180200220240260280300320340
    ccgaggccaagagcagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttctgcttcccgagctctatggctccggttctcgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagacgaagggctcgagataSacIAflIIBbvCIBpu10IBssHIIEco53kISFFV360380400420440460480500520
    aaaagagctcacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCttttctcgagtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCCGGTGCTCAAGCTCTAGAscIMscISacIBssHIIEco53kIHindIIIM1VS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG24SV26NG28HE30FE32I602-1309mCherrySFFV540560580600620640660680700
    GAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTACTTGAAGCTGTCCTTCCCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGATGAACTTCGACAGGAAGGStuIE34GE36GE38GR40PY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD82IP84DY86LK88LS90F602-1309mCherry720740760780800820840860
    CCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGAloIBplISbfIStuIPflMIP92EG94FK96WE98RV100MN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM140QK142KT144MG146WE148AS150602-1309mCherry8809009209409609801,0001,0201,040
    CTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAACCTGTAGTGGAGGGTGBplIKasIKasINarINarISfoISfoIBbvCIPluTIPluTIBpu10ISE152RM154YP156ED158GA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198VN200IK202LD204IT206SH208602-1309mCherry1,0601,0801,1001,1201,1401,1601,1801,2001,220
    AACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGACGTCGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCTGCAGCMluIXcmIBsrGISgrAIAflIIINE210DY212TI214VE216QY218ER220AE222GR224HS226TG228GM230DE232LY234KS236602-1309D1A2L3E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V271319-1417mCherry5' StopGo (2A; Loughran 2017)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCAAAAGATTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTTTTCTAAgeIBaeIKpnISpeIBamHIAcc65IE1A2R3H4K5Q6K7I81553-1651G1T2G1S2T1S2S1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T371430-1540T1G2E28S29N30P31G32P331319-1417ADAR substrate3xMS2 SGR5' stem3' StopG...n 2017)3' stem5' St...017)1,4201,4401,4601,4801,5001,5201,5401,560
    CGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCATGGAGACAGACACACTCCTGCTATGGGTACTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACGCTAGTGAAACGGGCGGATCAGGCGGTCGTCTGGGAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGTACCTCTGTCTGTGTGAGGACGATACCCATGACGACGAGACCCAAGGTCCAAGGTGACCACTGCGATCACTTTGCCCGCCTAGTCCGCCAGCAGACCCTBmrIBamHIPaqCIV9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P331553-1651ME2TD4TL6LL8WV10LL12LW14VP16GS18TG20DA22SE24TG26GS28GG30RL32G1652-26503' StopGo (2A; Loughran 2017)Igκ leadersgRNA_SasgRNA_Sphomolog1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TCCGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTAGGCTGTTTTGAGTGTGTACGGGTGGCACGGGTCGTGGACTTGAGGACCCCCCTGGCAGTCAGAAGGAGAAGGGGGGTTTTGGGTTCCTGTGGGAGTACTAGAGGGCCTGGGGACTCCAGTGTACGCACCACCACCTGCACTCGGTGCTTCTGGGACTCCAGTTCAAGTTGACCABmgBIBsu36IBsu36IS34DK36TH38TC40PP42CP44AP46EL48LG50GP52SV54FL56FP58PK60PK62DT64LM66IS68RT70PE72VT74CV76VV78DV80SH82ED84PE86VK88FN90W1652-2650hIgG1-Fc1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTTAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATTGCACCTGCCGCACCTCCACGTATTACGGTTCTGTTTCGGCGCCCTCCTCGTCATGTTGTCGTGCATGGCACACCAGTCGCAGGAGTGGCAGGACGTGGTCCTGACCGAATTACCGTTCCTCATGTTCACGTTCCAGAGGTTGTTTCGGGAGGGTCGGGGGTAGCTCTTTTGGTABsaIEcoNISacIIY92VD94GV96EV98HN100AK102TK104PR106EE108QY110NS112TY114RV116VS118VL120TV122LH124QD126WL128NG130KE132YK134CK136VS138NK140AL142PA144PI146EK148TI1501652-2650hIgG1-Fc1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTCTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGGAGGTTTCGGTTTCCCGTCGGGGCTCTTGGTGTCCAGATGTGGGACGGGGGTAGGGCCCTCCTCTACTGGTTCTTGGTCCAGTCGGACTGGACGGACCAGTTTCCGAAGATAGGGTCGCTGTAGCGGCACCTCACCCTCTCGTTACCCGTCGGCCTCTTGTTGATGTTCTGGTGCSexAISK152AK154GQ156PR158EP160QV162YT164LP166PS168RE170EM172TK174NQ176VS178LT180CL182VK184GF186YP188SD190IA192VE194WE196SN198GQ200PE202NN204YK206TT2081652-2650hIgG1-Fc2,1202,1402,1602,1802,2002,2202,2402,260
    CCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCACGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAAGGAGGAGGTGGAAGCGGGAGGGCACGACCTGAGGCTGCCGAGGAAGAAGGAGATGTCGTTCGAGTGGCACCTGTTCTCGTCCACCGTCGTCCCCTTGCAGAAGAGTACGAGGCACTACGTGCTCCGAGACGTGTTGGTGATGTGCGTCTTCTCGGAGAGGGACAGAGGCCCATTTCCTCCTCCACCTTCGCSapIXmnIBspQIPaqCIPP210VL212DS214DG216SF218FL220YS222KL224TV226DK228SR230WQ232QG234NV236FS238CS240VM242HE244AL246HN248HY250TQ252KS254LS256LS258PG260KG262GG264GS2661652-2650hIgG1-FcLinker2,2802,3002,3202,3402,3602,3802,4002,4202,440
    GAGGAGGAGGAAGCGGAGGAGGAGGTAGCggaGAACAAAAACTCATCTCAGAAGAGGATCTGGGCGGAGGAAGTGGATCAGGAGGATCTGGTTCCATCTACATCTGGGCCCCCCTTGCTGGAACATGCGGAGTGCTGCTGCTGAGCCTGGTGATCACCCTGTACTGCAACCACAGCTCCTCCTCCTTCGCCTCCTCCTCCATCGcctCTTGTTTTTGAGTAGAGTCTTCTCCTAGACCCGCCTCCTTCACCTAGTCCTCCTAGACCAAGGTAGATGTAGACCCGGGGGGAACGACCTTGTACGCCTCACGACGACGACTCGGACCACTAGTGGGACATGACGTTGGTGTCApaIBclIBlpIPspOMIGG268GG270SG272GG274GS276GE278QK280LI282SE284ED286LG288GG290SG292SG294GS296GS298IY300IW302AP304LA306GT308CG310VL312LL314SL316VI318TL320YC322NH324R1652-2650TM domainLinkerMyc2,4602,4802,5002,5202,5402,5602,5802,6002,620
    AAACAGGTCTGGTGGATCTGGAGGTtaggccggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccTTTGTCCAGACCACCTAGACCTCCAatccggcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggApaIBclIPmeIPspOMIN326RS328GG330SG332G1652-2650*1BGH polyadenylation sequencec-myc epitopePolyhi...e taghomolog2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgaSphIBGH polyadenylation sequence2,8202,8402,8602,8802,9002,9202,9402,960
    ctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgccgatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcggClaIBglIIBspDIBGH pA2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctgcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggaKflINaeINgoMIVFRT3,1603,1803,2003,2203,2403,2603,2803,3003,320
    attctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgtaagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcBarIMscIXbaIPshAIHygromycinFRT3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaaccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttEcoRIBstAPIDraIIIHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    ctgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgagacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactNdeIPvuIAsiSIRsrIIHygromycin3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggaacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctBstAPIDraIIIHygromycin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaataagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaAjuISacIIHygromycin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccScaIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    aatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaattatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttNaeIPsiINgoMIVSV40 pA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccaatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtBsmIBstZ17ISV40 pA4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcga4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctgaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaPciISapIBspQIAflIIIpUC ori4,9204,9404,9604,9805,0005,0205,0405,060
    ggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagpUC ori5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccacggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgpUC ori5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtpUC ori5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctagpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    cttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttacgaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatg*W286HK284IL282SA280GI278EA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252G5841-6701Amp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    catctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtgtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaBglIBmrIBsaID250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228WG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194T5841-6701Amp (bla)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    taatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcAclIFspIPvuIL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170EN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136L5841-6701Amp (bla)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcgBcgIScaIN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112LH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG765841-6701Amp (bla)6,3206,3406,3606,3806,4006,4206,4406,460
    cacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcagtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgtAclIXmnICL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC185841-6701Amp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    aaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgtttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacSspIFA16AF14FP12IL10AV8RF6HQ4IS2M5841-6701bla promoterAmp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggBglIISgrDI6,8406,8606,8806,900
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6912 bp500055006000650050010001500sgRNA_SaMutate out ESP3I sitesgRNA_Spopposite edited A1652-2650GRo0112.2 (DNA)EKo412.2 (DNA)EK_SFFV_F (DNA)EKo271.3 (DNA)EKo326.2 (DNA)EKo309.1 (DNA)Cit_1 (DNA)pNSK019 (DNA)XZp042.5 (DNA)SB004.4 (DNA)EKo387.3 (DNA)XZp087.2 (DNA)XZ87-test-F1 (DNA)XZp086.2 (DNA)BGHR Genewiz (DNA)EKo326.3 (DNA)EKo152.1 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)missing in pSLQ2349,missing in pSLQ2349,FlpInF1 (DNA),FlpInF (DNA),+8SFFV,A->T from pSLQ2349,G->A from pSLQ2349,EcoRV,BbvCI,Bpu10I,AflIIEK_SFFV_F2 (DNA),BssHII,Eco53kI,SacI,Eco53kIKozak,599-601,EKo271.2 (DNA),XZp054.1 (DNA),Che_5 (DNA),+5Che_8 (DNA)EKo230.1 (DNA)mChe_4 (DNA),mChe_1 (DNA),StuImChe_3 (DNA),pNSK093 (DNA)mCherry,602-1309,Che_6 (DNA),SbfI,AloIBplI,PflMI,StuIBplI,KasI,NarI,SfoI,PluTIBbvCI,Bpu10INK-cherry-seq (DNA),NK-cherry-seq (DNA),+6Che_7 (DNA)1310-1318,XZp073.1 (DNA),pNSK091 (DNA),+105' StopGo (2A; Loughran 2017),1319-1417,+11418-1423,1424-1429,XZ071-test-R (DNA),+75' stem,bulged A -- edited?,bulged,+1ADAR substrate,3xMS2 SGR,loop MS2,+9mutation site,1541-1546,1547-1552,+73' StopGo (2A; Loughran 2017),+2homolog,pNSK126 (DNA)Igκ leaderMutate out NHEI cut sitepUC oriBmrI,BsaIBglIFspI,AclIAmp (bla),5841-6701PvuIScaI,BcgIAclI,XmnIbla promoter,SspI,SgrDI,BglII

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    6912

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