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pv9-MBD1.MBD-R22A-13xL-BASU
  • Sequence Map
    Editing disabled on read-only sequence.
    tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagcgcgcaaagccactactgccacttttggagactgtgtacgtcgagggcctctgccagtgtcgaacagacattcgcctacggccctcgtctgttcgggcagtcccgcgcagtcgcccacaaccgcccacagccccgaccgaattgatacgccgtagtctcgtctaacatgactcGEX3-revGEX3-rev-2520406080100120140160
    agtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtatcacgtggtatacgccacactttatggcgtgtctacgcattcctcttttatggcgtagtccgcggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccatFspINdeIPvuIrv060pUCM13-52180200220240260280300320340
    acgccagggttttcccagtcacgacgttgtaaaacgacggccagtccataacttcgtataatgtatactatacgaagttatatcgGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTAtgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcaggtattgaagcatattacatatgatatgcttcaatatagcCTGTAACTAATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTGAATAhdISpeICMV enhancerCAG (not sequenced!)rv141rv142L1usL1bM13-40M13360380400420440460480500520
    CGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGNdeICMV enhancerCAG (not sequenced!)CAG 5' r540560580600620640660680700
    CCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCAGCTCCACTCGGGGTGCAAGACGAAGTGAGAGGGGTAGAGGGGGGGGAGGGGTGGGGGTNcoIBtgZISnaBICAG (not sequenced!)CMV enhancer720740760780800820840860
    ATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGTAAAACATAAATAAATAAAAAATTAATAAAACACGTCGCTACCCCCGCCCCCCCCCCCCCCGCGCGCGGTCCGCCCCGCCCCGCCCCGCTCCCCGCCCCGCCCCGCTCCGCCTCTCCACGCCGCCGTCGGTTAGTCTCGCCGCGCGAGGCTTTCAAAGGAAAATACCGCTCCGCCBsgIBtgZICAG (not sequenced!)8809009209409609801,0001,0201,040
    CGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGTTGCCTTCGCCCCGTGCCCCGCTCCGCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCCGCCGCCGCCGGGATATTTTTCGCTTCGCGCGCCGCCCGCCCTCAGCGACGCAACGGAAGCGGGGCACGGGGCGAGGCGCGGCGGAGCGCGGCGGGCGGGGCCGAGACTGACTGGCGCAATGAGGGTGTCCACTCGCCCGCCCTGCCGGGAAGAGGAGGCCCGACATTAATCGCAG (not sequenced!)replace with 2xTetO1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GCTTGGTTTAATGACGGCTCGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTAAAGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGCGGCCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGAACCAAATTACTGCCGAGCAAAGAAAAGACACCGACGCACTTTCGGAATTTCCCGAGGCCCTCCCGGGAAACACGCCCCCCCTCGCCGAGCCCCCCACGCACGCACACACACACGCACCCCTCGCGGCGCACGCCGGGCGCGACGGGCCGCCGACACTCGCGACGCCCGCGCCAfeIAfeICAG (not sequenced!)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CGCGGGGCTTTGTGCGCTCCGCGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGTGTGGGCGCGGCGGTCGGGCTGTAACCCCCCCCTGCACCCCCCTCGCGCCCCGAAACACGCGAGGCGCACACGCGCTCCCCTCGCGCCGGCCCCCGCCACGGGGCGCCACGCCCCCCCGACGCTCCCCTTGTTTCCGACGCACGCCCCACACACGCACCCCCCCACTCGTCCCCCACACCCGCGCCGCCAGCCCGACATTGGGGGGGGACGTGGGGGGAGBsgICAG (not sequenced!)1,4201,4401,4601,4801,5001,5201,5401,560
    CCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTGCGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCGGAGCGCCGGCGGCGGGCTCAACGACTCGTGCCGGGCCGAAGCCCACGCCCCGAGGCACGCCCCGCACCGCGCCCCGAGCGGCACGGCCCGCCCCCCACCGCCGTCCACCCCCACGGCCCGCCCCGCCCCGGCGGAGCCCGGCCCCTCCCGAGCCCCCTCCCCGCGCCGCCGGGGCCTCGCGGCCGCCGBlpIMreISgrAICAG (not sequenced!)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGCGAAGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGACAGCTCCGCGCCGCTCGGCGTCGGTAACGGAAAATACCATTAGCACGCTCTCCCGCGTCCCTGAAGGAAACAGGGTTTAGACCGCCTCGGCTTTAGACCCTCCGCGGCGGCGTGGGGGAGATCGCCCGCGCCCGCTTCGCCACGCCGCGGCCGTCCTTCCTTTACCCGCCCCTCCAG (not sequenced!)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCATCTCCAGCCTCGGGGCTGCCGCAGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACACCGGAAGCACGCAGCGGCGCGGCGGCAGGGGAAGAGGTAGAGGTCGGAGCCCCGACGGCGTCCCCCTGCCGACGGAAGCCCCCCCTGCCCCGTCCCGCCCCAAGCCGAAGACCGCACACTGGCCGCCGAGATCTCGGAGACGATTGGTACAAGTACGGAAGAAGAAAAAGGATGTXbaICAG (not sequenced!)homologousRegionseq1fwd betterrp022_CR002_fwd1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GCTCCTGGGCAACGTGCTGGTTGTTGTGCTGTCTCATCATTTTGGCAAAGAATTCattattgtttttcctcctttGCCGCCACCATGGACTACAAAGACGATGACGACAAGGATgaaGAGTCTGGTTCTGTTtctggtggtgatGCTGAGTCCTGGCAGGACTGCCCAGCCCTGGCGAGGACCCGTTGCACGACCAACAACACGACAGAGTAGTAAAACCGTTTCTTAAGtaataacaaaaaggaggaaaCGGCGGTGGTACCTGATGTTTCTGCTACTGCTGTTCCTActtCTCAGACCAAGACAAagaccaccactaCGACTCAGGACCGTCCTGACGGGTCGGGACCNcoIPasIAlwNIEcoRIMD2YK4DD6DD8KD10EE12SG14SV16SG18GD20AE22SW24QD26CP28AL302185-2784seq1Mbd1_MBDGibsonFrv164_gibsonF_fwdrv043rv040Kos...us2,1202,1402,1602,1802,2002,2202,2402,260
    GCCCTGGCTGGAAACGCCGAGAGTCCTTTGCAAAGTCAGGGGCCTCGTTTGGACGCTCAGACATCTATTACCAGAGCCCCACAGGAGAAAAGATCCGAAGCAAAGTTGAGCTGACTCGGTACTTGGGCCCTGCATGTGATCTTACCCTGTTTGACTTCAGACAAGGCACCTTATGCGGGACCGACCTTTGCGGCTCTCAGGAAACGTTTCAGTCCCCGGAGCAAACCTGCGAGTCTGTAGATAATGGTCTCGGGGTGTCCTCTTTTCTAGGCTTCGTTTCAACTCGACTGAGCCATGAACCCGGGACGTACACTAGAATGGGACAAACTGAAGTCTGTTCCGTGGAATACGP32GW34KR36RE38SF40AK42SG44AS46FG48RS50DI52YY54QS56PT58GE60KI62RS64KV66EL68TR70YL72GP74AC76DL78TL80FD82FR84QG86TL88C2185-2784seq1Mbd1_MBD2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCATCCCatcggtggcggaggctctggaggtggaggctccgcaCCCCCCAAAAAGAAACGCAAGGTTGGAtaccgggatccacacgtgGGTAGGAGCGgttCGttcggatcccatcttttttcggaattcggatccggtggaggcgggtctggaggcgggggtagtggcgggggtGGTAGGGtagccaccgcctccgagacctccacctccgaggcgtGGGGGGTTTTTCTTTGCGTTCCAACCTatggccctaggtgtgcacCCATCCTCGCcaaGCaagcctagggtagaaaaaagccttaagcctaggccacctccgcccagacctccgcccccatcaccgcccccaPmlIXcmIEcoRIH90PI92GG94GG96SG98GG100GS102AP104PK106KK108RK110VG112YR114DP116HV118GR120SG122SF124GS126HL128FS130EF132GS134GG136GG138SG140GG142GS144GG146G2185-2784seq1ce001_Chromo/Link13X-LinkerGA rev pCR002rp023_CR2_13XLrevGA fwd, pv8 (rp012)GibsonRnlsce001_bsrv_229_...nR_fwdGA overhangrv044rev pCR0022,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggaagcgggggtggaggcgggtcgggtggcggaggtagcggaggcggtggaagtggaggcggtggaagtggtggaggaggttctggtggcggaggtagcggtggcggaggtagcggaggcggtggaagtggtggcggaggtagcggaggcggtggatcggcgcgccgtttcaagaccttcgcccccacctccgcccagcccaccgcctccatcgcctccgccaccttcacctccgccaccttcaccacctcctccaagaccaccgcctccatcgccaccgcctccatcgcctccgccaccttcaccaccgcctccatcgcctccgccacctagccgcgcggcaaagttctAscIG148SG150GG152GG154SG156GG158GS160GG162GG164SG166GG168GS170GG172GG174SG176GG178GS180GG182GG184SG186GG188GS190GG192GG194SG196GG198GS2002185-278413X-Linkerseq1rev p...oID1rp007...BASU2,6402,6602,6802,7002,7202,7402,7602,7802,800
    actacccatacgatgtgcctgactatgctggcaagttgtctgaatcagagattaggtttggactgaaaacggaggtgatgggccaacatcttatctatcacgatgttttgtccagcacacaaaagacagcccatgaattggcaaataacaatgcccccgagggtaccctcgtggttgatgggtatgctacacggactgatacgaccgttcaacagacttagtctctaatccaaacctgacttttgcctccactacccggttgtagaatagatagtgctacaaaacaggtcgtgtgttttctgtcgggtacttaaccgtttattgttacgggggctcccatgggagcaccaKpnIPflMIAcc65IGK2LS4ES6EI8RF10GL12KT14EV16MG18QH20LI22YH24DV26LS28ST30QK32TA34HE36LA38NN40NA42PE44GT46LV48V2830-3609BASU*rp007_Link_BASUrp006_BASU_fHA Tag2,8202,8402,8602,8802,9002,9202,9402,960
    tgcagataaacagacagccggtaggggcgggatgagccgagtgtggcattctcaagaaggaaacggagtatggatgagtttgatattgcgcccggacattccgctccaaaagacgccccaacttaccttgctggcagctgtagcggtggttcagggaatagaagaggccgcgggcacgtctatttgtctgtcggccatccccgccctactcggctcacaccgtaagagttcttcctttgcctcatacctactcaaactataacgcgggcctgtaaggcgaggttttctgcggggttgaatggaacgaccgtcgacatcgccaccaagtcccttatcttctccggcgcccgBaeIA50DK52QT54AG56RG58GM60SR62VW64HS66QE68GN70GV72WM74SL76IL78RP80DI82PL84QK86TP88QL90TL92LA94AV96AV98VQ100GI102EE104AA106G2830-3609BASU*2,9803,0003,0203,0403,0603,0803,1003,1203,140
    atacagactgatataaagtggccgaacgacatactcattaacgggaaaaaaacagtagggatacttaccgaaatgcaagctgaggaggatcgagtgcgctccgtgattattggtattggcattaatgtaaatcagcaacccaatgatttccccgatgagctcaaggacattgcgatatgtctgactatatttcaccggcttgctgtatgagtaattgccctttttttgtcatccctatgaatggctttacgttcgactcctcctagctcacgcgaggcactaataaccataaccgtaattacatttagtcgttgggttactaaaggggctactcgagttcctgtaacgctSacIBbvCIBpu10IEco53kII108QT110DI112KW114PN116DI118LI120NG122KK124TV126GI128LT130EM132QA134EE136DR138VR140SV142II144GI146GI148NV150NQ152QP154ND156FP158DE160LK162DI164A2830-3609BASU*3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cgagtctgtcccaggcagctggggaaaagatcgacagggcaggtgtaatccagcatatcttgctttgtttcgaaaagcgataccgagactacatgacccacggtttcacgcccatcaagctcttgtgggagtcatacgccctcggtattggcacgaacatgagggcgagaactctgctcagacagggtccgtcgaccccttttctagctgtcccgtccacattaggtcgtatagaacgaaacaaagcttttcgctatggctctgatgtactgggtgccaaagtgcgggtagttcgagaacaccctcagtatgcgggagccataaccgtgcttgtactcccgctcttgagaBstBIBstXIT166SL168SQ170AA172GE174KI176DR178AG180VI182QH184IL186LC188FE190KR192YR194DY196MT198HG200FT202PI204KL206LW208ES210YA212LG214IG216TN218MR220AR222TL2242830-3609BASU*3,3403,3603,3803,4003,4203,4403,4603,4803,500
    caacgggacattttacggtaaggcgttgggtattgacgacgaaggggtacttttgttggaaactaatgaaggcattaagaagatatacagcgcggacatcagccttaggTGAAACGAGGTAGCGGCCGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATgttgccctgtaaaatgccattccgcaacccataactgctgcttccccatgaaaacaacctttgattacttccgtaattcttctatatgtcgcgcctgtagtcggaatccACTTTGCTCCATCGCCGGCGGCTTCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATABaeINotIBsu36I*1NG226TF228YG230KA232LG234ID236DE238GV240LL242LE244TN246EG248IK250KI252YS254AD256IS258LR2602830-3609BASU*GA rev, BASU (rp014)rp014_BASU_v8_rIREStb288rev primer BASUIRES-rev3,5203,5403,5603,5803,6003,6203,6403,660
    TTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGBbsIBbsIXmnIAvrIIHindIIIIRESrp019_IRESrev3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCPmlIDraIIIIRESrv0223,8603,8803,9003,9203,9403,9603,9804,0004,020
    GATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGACCGAGTACAAGCCCACTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCACGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTATTATACCGGTGTTGGTACTGGCTCATGTTCGGGTKpnIMscIPciIBmgBIPflMIAcc65IV1V2F3L4*5K6T7R8*9*10Y114139-4172MA2TT4MT6EY8KP104170-4781IRESpuroR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    CGGTGCGCCTCGCCACCCGCGACGACGTCCCCCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACSalISmaIXmaIBsiWIPflFIRsrIITspMIBstEIIHincIITth111ITV12RL14AT16RD18DV20PR22AV24RT26LA28AA30FA32DY34PA36TR38HT40VD42PD44RH46IE48RV50TE52LQ54EL56FL58TR60VG62LD64IG66KV68W4170-4781puroRrv1174,2204,2404,2604,2804,3004,3204,3404,360
    GGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCStuIV70AD72DG74AA76VA78VW80TT82PE84SV86EA88GA90VF92AE94IG96PR98MA100EL102SG104SR106LA108AQ110QQ112ME114GL116LA118PH120RP122KE124PA126W4170-4781puroR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    TTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCBcgIBsaIMscIBsaXIF128LA130TV132GV134SP136DH138QG140KG142LG144SA146VV148LP150GV152EA154AE156RA158GV160PA162FL164ET166SA168PR170NL172PF174YE176RL178GF180TV182TA184D4170-4781puroR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    TCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAGGAGCGCAGTGCTACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTGCGGGCGGGGTGCTGGGCGTCGCGGGCTGGCTTTCCTCGCGTCACGATGCTCTAAAGCTAAGGTGGCGGCGGAAGATACTTTCCAACCCGAAGCCTTAGCAAAAGGCCCTGCGGCCGACSexAIDraIIIV186EV188PE190GP192RT194WC196MT198RK200PG202A*2044170-4781puroRrv023rv0594,7404,7604,7804,8004,8204,8404,8604,8804,900
    GATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATCTACTAGGAGGTCGCGCCCCTAGAGTACGACCTCAAGAAGCGGGTGGGGTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTAPsiISV40 polyApolAfwdpCI-revEBV-revEGFP-C-Rev4,9204,9404,9604,9805,0005,0205,0405,060
    GTCTGTATACCGTGGATccggccggccccataacttcgtatagtatacattatacgaagttatcccctgcaggcatgcaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaaCAGACATATGGCACCTAggccggccggggtattgaagcatatcatatgtaatatgcttcaataggggacgtccgtacgttcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttFseIPstISbfISphIHindIII1LLacOp-forpUCM13-rev-157M13rSV40...lyA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgaSapIBsaXIBspQIds1Lb5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctPciI5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagt5,6205,6405,6605,6805,7005,7205,7405,760
    cgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagaggcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcAlwNI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttggctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaac5,9605,9806,0006,0206,0406,0606,0806,1006,120
    caagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttag6,1406,1606,1806,2006,2206,2406,2606,2806,300
    taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctcAhdIBsaIAmpStop6,3206,3406,3606,3806,4006,4206,4406,460
    acccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctactgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgAclIFspI6,4806,5006,5206,5406,5606,5806,6006,6206,640
    aggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcatccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtPvuI6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattgcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaacBcgIScaI6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgcttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcAclIXmnIAmpStart7,0207,0407,0607,0807,1007,1207,1407,160
    gaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagattctttggtaataatagtacSspI7,1807,2007,2207,2407,2607,2807,3007,3207,340
    acattaacctataaaaataggcgtatcacgaggccctttcgtctgtaattggatatttttatccgcatagtgctccgggaaagcagQE-for7,3607,380
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7393 bp550060006500700050010001500CAG (not sequenced!)replace with 2xTetOtranscript startAmpStoprv142GEX3-rev-25,GEX3-rev,NdeI,FspI,PvuIrv060,pUCM13-52,usL1b,M13-40,rv141,M13,L1,AhdI,SpeICMV enhancer,CAG 5' r,NdeIBtgZI,SnaBI,NcoIBtgZIBsgITATAAAAfeIAfeIBsgIBlpIMreISgrAIAlwNIAhdIBsaIFspI,AclIPvuIScaI,BcgI,AclI,XmnIAmpStart,QE-for,SspI

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    7393