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Insert_loader_into_T4pol-del
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAZraIAatIIBciVIBsaHIEcoO109IpUC19-fseq 20406080100120140160
    TCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGApaLIBaeGIbla genebla signal peptide180200220240260280300320340
    AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAATCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTScaIBsaHIbla geneBcgIXm...I360380400420440460480500520
    AGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGAPvuIbla gene540560580600620640660680700
    GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTCTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGABglIFspIBsrDINmeAIIIbla geneBglI720740760780800820840860
    GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCAAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATAhdIBanIBpmIBsrDIBsrFIbla genepUC-remBSA-F pUC-remBSA-R Ea...I8809009209409609801,0001,0201,040
    AGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCbl...e1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTATAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATBceAIrep originRNAI transcript1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCACCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTMmeIAlwNIApaLIBaeGIBseYIrep origin1,4201,4401,4601,4801,5001,5201,5401,560
    GCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAMmeIBciVIrep origin1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGDrdIPciIrep originDrd...DI1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCACGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTSapIBsaXIBspQIBsaXI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCcagtaaagatgcgaaaaagattcctactgtcgattttgaccTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGgtcatttctacgctttttctaaggatgacagctaaaactggBanIY176LH174SF172SE170*Q168RN166QG1642235-2764seq verifiedH11Hom3-trunc-loc2 pUC19-rseqCAP...nd2,1202,1402,1602,1802,2002,2202,2402,260
    cacgcatcaaggcgataaaccatggaagcatgtatataataatagccaacacgcctgaaactaaaaccataaaaattgaacctgctactaaagtttccatgttttcctcacttaggtcaaattttttacacatgaattataagaattcactacatactccatcggagcgtttttagtgcgtagttccgctatttggtaccttcgtacatatattattatcggttgtgcggactttgattttggtatttttaacttggacgatgatttcaaaggtacaaaaggagtgaatccagtttaaaaaatgtgtacttaatattcttaagtgatgtatgaggtagcctcgcaaaaatBtgINcoIStyIBfuAIBspMIEcoNIEcoRIVC162*P160SL158GH156FC154TY152LL150LW148CA146QF144*F142WL140FQ138VQ136**134LK132WT130KR128V*126TL124NK122VC120SN118YS116NV114VY112EM110PA108NK1062235-2764seq verifiedH112,2802,3002,3202,3402,3602,3802,4002,4202,440
    cctgtacgccactggtaattattagcccaatttgcccaaagctcagcgcagtagttttcaattttttcttcgcgtgtaattacatctgaattacgatatgcttgagcagattcatctggacgaatagcttcgtcaaaattcgcctgcatttgttctactgtctgttttggagcttggacatgcggtgaccattaataatcgggttaaacgggtttcgagtcgcgtcatcaaaagttaaaaaagaagcgcacattaatgtagacttaatgctatacgaactcgtctaagtagacctgcttatcgaagcagttttaagcggacgtaaacaagatgacagacaaaacctcgaaBlpIGT104RW102QY100NN98AW96NA94WL92EA90CY88NE86IK84EE82RT80IV78DS76NR74YA72QA70SE68DP66RI64AE62DF60NA58QM56QE54VT52QK50PA48E2235-2764seq verifiedH112,4602,4802,5002,5202,5402,5602,5802,6002,620
    ctttataacacttgacattaggattataaaatttgcttgaacagtttacaatttttcctacatcagactgatttactaccggtccttgagctacacaaccagcaagacctaatgcaataaccaaaatagcgattttcatNNNNNNNNNNNNCCATnnnnnnnACGAGTCAAAGTTgaaatattgtgaactgtaatcctaatattttaaacgaacttgtcaaatgttaaaaaggatgtagtctgactaaatgatggccaggaactcgatgtgttggtcgttctggattacgttattggttttatcgctaaaagtaNNNNNNNNNNNNGGTAnnnnnnnTGCTCAGTTTCAAAgeIBsrFIK46YC44KV42NP40NY38FK36SS34CN32VI30KG28VD26SQ24NV22VP20GQ18AV16CG14AL12GL10AI8VL6IA4IK2M2235-2764seq verifiedH11H11.fwd.barcodegH11.bk.H112,6402,6602,6802,7002,7202,7402,7602,7802,800
    TCTTTTACTTTCAGAAAATCTTCAGGTTTTTTAAGAGTAATTTCAATCATTTTACCATTCCAATGCTAGTTTTTTGAGTTGTTTCTGTTCTTTTACGTTCTTAGTCACTTCTTTCAAAAAATCATCCGTGACTAAACCTTTTAGTTCTTTTAATACTAAAGGAAGTTTTCCATTTAGAAAATGAAAGTCTTTTAGAAGTCCAAAAAATTCTCATTAAAGTTAGTAAAATGGTAAGGTTACGATCAAAAAACTCAACAAAGACAAGAAAATGCAAGAATCAGTGAAGAAAGTTTTTTAGTAGGCACTGATTTGGAAAATCAAGAAAATTATGATTTCCTTCAAAAGGTAAA*188WE186LA184LK182KL180QK178QE176KV174NK172TV170EK168LF166DD164TV162LG160KL158EK156LV154LP152LK150GN1482852-3415seq verifiedGP62gap_bk_p11_2Xm...I2,8202,8402,8602,8802,9002,9202,9402,960
    TTAGTAAGAATTGATTTATAGTTAATTGCATCATTTGTATTAACTTGATACCGCTTAGCAAGTAACTTAATAATCAATACTTCGGTGGAATCTTCAACCAGTTTTGCCCATTTACCATATCTTTTACCACGAGGAACTGCAGCCATTAGATAATTAAAATGAGCTTCATCACTTAAATCATTCTTAACTAAATATCAATTAACGTAGTAAACATAATTGAACTATGGCGAATCGTTCATTGAATTATTAGTTATGAAGCCACCTTAGAAGTTGGTCAAAACGGGTAAATGGTATAGAAAATGGTGCTCCTTGACGTCGGTAATCTATTAATTTTACTCGAAGTAGTGAATBlpIPstIAflIIKT146LI144SK142YN140IA138DN136TN134VQ132YR130KA128LL126KI124IL122VE120TS118DE116VL114KA112WK110GY108RK106GR104PV102AA100ML98YN96FH94AE92DS90L2852-3415seq verifiedGP622,9803,0003,0203,0403,0603,0803,1003,1203,140
    AGCCTGATCCAATTAAATTCATAGCATATACAGCTGGCATACACTCTGGAAATTGTGATAATGCATTTTCAACCATGAATTTTGAATAATCTTTTTGAGCAATAGAGCATTTAGTTTTATTATTAATAGCTCCAATTATTTCAAAAAATTCATTTTCTGCTTTTTCTTTAAAAGATCGGACTAGGTTAATTTAAGTATCGTATATGTCGACCGTATGTGAGACCTTTAACACTATTACGTAAAAGTTGGTACTTAAAACTTATTAGAAAAACTCGTTATCTCGTAAATCAAAATAATAATTATCGAGGTTAATAAAGTTTTTTAAGTAAAAGACGAAAAAGAAATTTTCTNsiIG88SG86IL84NM82AY80VA78PM76CE74PF72QS70LA68NE66VM64FK62SY60DK58QA56IS54CK52TK50NN48IA46GI44IE42FF40EN38EA36KE34KF32S2852-3415GP62seq verifiedseq verified3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ATCAGCAGCGGATTGGACAGCTGTCCAATCTTTTGAATACCAAGCAACTTGATGCTCGTTTAATTGAATATCATCTTTAAATAAGCTCATATCACTTCCACTGCATTTCGCATGCTAATTGAATGAAAAGATAAGCTAAATGCAATTCAGTATTAGCTGCAATACCATGATACTGTAGTCGTCGCCTAACCTGTCGACAGGTTAGAAAACTTATGGTTCGTTGAACTACGAGCAAATTAACTTATAGTAGAAATTTATTCGAGTATAGTGAAGGTGACGTAAAGCGTACGATTAACTTACTTTTCTATTCGATTTACGTTAAGTCATAATCGACGTTATGGTACTATGACSphIPshAID30AA28SQ26VA24TW22DK20SY18WA16VQ14HE12NL10QI8DD6KF4LS2M2852-3415*320KW318QM316EC314AL312QI310FL308YA306LH304LE302TN300AA298IG296HY294Q3417-4376seq verifiedGP62GP44homo1_f1Ps...I3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ATTATTTTCGCCGACAATTTCGTACATACGAATAATACTTTGTGGAGTTACACGTGAATAGATTTCTTCGGCAAGTTTACCCACGAACCACGAATAATCAGCCGCATATTTTGGTGCTAAAGCTCTGAGTTGTTTAACATCTTTATTTTTGAGAGACTCAAGAACATCATCAATATAATAAAAGCGGCTGTTAAAGCATGTATGCTTATTATGAAACACCTCAATGTGCACTTATCTAAAGAAGCCGTTCAAATGGGTGCTTGGTGCTTATTAGTCGGCGTATAAAACCACGATTTCGAGACTCAACAAATTGTAGAAATAAAAACTCTCTGAGTTCTTGTAGTAGTTATPmlIBsaAIN292NE290GV288IE286YM284RI282IS280QP278TV276RS274YI272EE270AL268KG266VF264WS262YD260AA258YK256PA254LA252RL250QK248VD246KN244KL242SE240LV238DD236I3417-4376seq verifiedGP44spacer 13,5203,5403,5603,5803,6003,6203,6403,660
    GCACCACGATCGTTAGTAACCAGTGATAAAATACCAGCATCCAAAACACCTTTAGACGAATAACTATCGAGCTCGCCAATAGTTTTACGAAAATCAGGAAAATTCTTTTTAACCAAAGCTGCTACAACTTTCATATCAGCTATAGCAATTCCTTCATGCTTGCAGATTTCAGTCACGTGGTGCTAGCAATCATTGGTCACTATTTTATGGTCGTAGGTTTTGTGGAAATCTGCTTATTGATAGCTCGAGCGGTTATCAAAATGCTTTTAGTCCTTTTAAGAAAAATTGGTTTCGACGATGTTGAAAGTATAGTCGATATCGTTAAGGAAGTACGAACGTCTAAAGTCAGTPvuISacIBanIIEco53kIA234GR232DN230TV228LS226LI224GA222DL220VG218KS216SY214SD212LE210GI208TK206RF204DP202FN200KK198VL196AA194VV192KM190DA188IA186IG184EH182KC180IE178TL1763417-4376seq verifiedGP44tentative AAA+Mseq-gp44-151-R spacer 2Spacer 3spacer 4Mseq.G...B2.R BcgI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ATCGACGAATCATCTGCTTCATCATTTCAATTTTATCTTCATCAGTTGGTTGACCGAATGTAATAACTCGGCAGCGTGACTGAAGCGGTTTAATAATACCATCAATATTATTAGCAGTAATAATAATACTACAGTTTGAACTATAAGCTTCCATAAAGGAACGAAGATGTCGCTGTAGCTGCTTAGTAGACGAAGTAGTAAAGTTAAAATAGAAGTAGTCAACCAACTGGCTTACATTATTGAGCCGTCGCACTGACTTCGCCAAATTATTATGGTAGTTATAATAATCGTCATTATTATTATGATGTCAAACTTGATATTCGAAGGTATTTCCTTGCTTCTACAGCGACHincIIHindIIINmeAIIIRR174IM172QK170MM168EI166KD164ED162TP160QG158FT156IV154RC152RS150QL148PK146II144GD142IN140NA138TI136II134SC132NS130SY128AE126MF124SR122LH120RQ1183417-4376seq verifiedGP44tentative AAA+Mseq-gp44-151-F Mseq.G...fd.R BcgI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    AGACTCTGCTAACCCTGAACGGTCAAATTCATCAATAACGATTACTTTTTGACGACCATCAAATGAAGCGGCGCTGGCAAAATTAGTCAAAGGACCACGAACGAAATCAATTTTACAATCTGACCCATTCACAAACATCATATCAGCATTTACATCATGACATAATGCTTTTGCTTCTGAGACGATTGGGACTTGCCAGTTTAAGTAGTTATTGCTAATGAAAAACTGCTGGTAGTTTACTTCGCCGCGACCGTTTTAATCAGTTTCCTGGTGCTTGCTTTAGTTAAAATGTTAGACTGGGTAAGTGTTTGTAGTATAGTCGTAAATGTAGTACTGTATTACGAAAACGASE116AL114GS112RD110FE108DI106VI104VK102QR100GD98FS96AA94SA92FN90TL88PG86RV84FD82IK80CD78SG76NV74FM72MD70AN68VD66HC64LA62KA603417-4376GP44tentative AAA+DEXX-R spacer12...r) cas9DEXX-F Mseq....2.F Mseq....F 4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ACAGTTGTTTTACCTGTTCCTGGAGAAGGAGAATGAAGAATAATATGTGGAATCTTACCTTTACTTGTAATAGATTTAAAGGTTTCTTTATCAAAAGCGGGAAGAATACATTCATCGATAGTAGATGGACGATATTTCTGTTCAAGAATGTGTTCTTTTTCATTTACAGTAATCATGTCAACAAAATGGACAAGGACCTCTTCCTCTTACTTCTTATTATACACCTTAGAATGGAAATGAACATTATCTAAATTTCCAAAGAAATAGTTTTCGCCCTTCTTATGTAAGTAGCTATCATCTACCTGCTATAAAGACAAGTTCTTACACAAGAAAAAGTAAATGTCATTAGTBpmIClaIPfoIBspDIEcoNIVT58TK56GT54GP52SP50SH48LI46IH44PI42KG40KS38TI36SK34FT32EK30DF28AP26LI24CE22DI20TS18PR16YK14QE12LI10HE8KE6NV4TI2M3417-4376GP44tentative AAA+homo1_r1Ec...IXm...IXm...I4,2204,2404,2604,2804,3004,3204,3404,360
    TAATTTCCTCATTCAAGTTTTAGTGTAAATTATAAAGGCCGAAGCCCTCTATTAAAAATCGTGGGTAGAATCAGCTTCAAGAGCTACCACATAATTCGCGTGTTCACCTTCAAATTTAGCAGCACCTTGTTTACCTTTTGCCCAAAGCAGAAGTTTATAATTTCCTGGTTGCATTATTAAAGGAGTAAGTTCAAAATCACATTTAATATTTCCGGCTTCGGGAGATAATTTTTAGCACCCATCTTAGTCGAAGTTCTCGATGGTGTATTAAGCGCACAAGTGGAAGTTTAAATCGTCGTGGAACAAATGGAAAACGGGTTTCGTCTTCAAATATTAAAGGACCAACGTAAGP45Mseq.GP44.B1.F homo2_f1termina...review4,3804,4004,4204,4404,4604,4804,5004,5204,540
    TTCATATTTGCCATATTGATAATGAAATTAAATGTATTTTCACCATCATAATCACCAAGAGTCAAAGAATATTTAACCCGGGTCAGAGCAGAATCTTCTACTTTATTAAAACCGTTAATTACGATTTTACCTTCTTTTACCGTGATAGCAATTGTATCAATTTGCAGACCACGAGAAGTATAAACGGTATAACTATTACTTTAATTTACATAAAAGTGGTAGTATTAGTGGTTCTCAGTTTCTTATAAATTGGGCCCAGTCTCGTCTTAGAAGATGAAATAATTTTGGCAATTAATGCTAAAATGGAAGAAAATGGCACTATCGTTAACATAGTTAAACGTCTGGTGCTCAvaIMfeISmaIXmaIBsoBITspMIGP45Mseq-GP45-R 4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ATACACGCAACAGCTGTTGAAGGTCTTCAGCTTTAATTTCAGTAACAGCAGATGCTACCGGGAATGGAATTGGTTTATTAGGAGCAACTACTGTACTCGGATCGGCTGCTGGCCAAAAAATTGTTGAGCGGGCATCAGCAATTTTAATATTTCCATCTTCTGACTGGGAAATTTCTATGTGCGTTGTCGACAACTTCCAGAAGTCGAAATTAAAGTCATTGTCGTCTACGATGGCCCTTACCTTAACCAAATAATCCTCGTTGATGACATGAGCCTAGCCGACGACCGGTTTTTTAACAACTCGCCCGTAGTCGTTAAAATTATAAAGGTAGAAGACTGACCCTTTAAAGBbsIMscIXcmIGP45Mseq-GP45-F XcmIXm...I4,7404,7604,7804,8004,8204,8404,8604,8804,900
    TGCATCATCATTAACTAAAGACAGAATACCGAGAAAACCGTTCAAATCGTAAATTGCTACATCAAAATCAATAACGTCAGAAATATTTGCTTCCGCATAAGTTGTACCATTAACTGCGCGAGTCATAATAAATTGACCGGATTTAAGCATAATACCAGAGTTAATAGTAGCGAAAACGTAGTAGTAATTGATTTCTGTCTTATGGCTCTTTTGGCAAGTTTAGCATTTAACGATGTAGTTTTAGTTATTGCAGTCTTTATAAACGAAGGCGTATTCAACATGGTAATTGACGCGCTCAGTATTATTTAACTGGCCTAAATTCGTATTATGGTCTCAATTATCATCGCTTTGP454,9204,9404,9604,9805,0005,0205,0405,060
    TTTTTAAGCAGAGCAGTAGTATCTTTAGACAGTTTCATGTAATTTCCTTCAATTCAAATGAGATTTAATTTTATAACTAATTTAATAAAGCAATTAACGATTAAAATCAGCCGCAATTGTTTCCGCAACAATTTGAGCAGCAACAATTAGACGTTCATCTGCGGGGTAATATCAGAAAAATTCGTCTCGTCATCATAGAAATCTGTCAAAGTACATTAAAGGAAGTTAAGTTTACTCTAAATTAAAATATTGATTAAATTATTTCGTTAATTGCTAATTTTAGTCGGCGTTAACAAAGGCGTTGTTAAACTCGTCGTTGTTAATCTGCAAGTAGACGCCCCATTATAGTCMfeIBsaBIGP45GAP2-Trans-clmp-R H4-fwd-GAP-clamp Bs...I5,0805,1005,1205,1405,1605,1805,2005,2205,240
    AAAGAGACAAAACCCATGCTCGACGAATTTTATTTTGGTTTTGTGGATTTGATTCAGAACGCACGTGTTTTGGCTGAATATCTTTTACATCAACAGTATAATTCACAGTAATTTTAGTCATAATACACCTTTAGTCATAATAATCAGTAACAGTCCAAGCTTCATTTCTATTGGATTTCTCTGTTTTGGGTACGAGCTGCTTAAAATAAAACCAAAACACCTAAACTAAGTCTTGCGTGCACAAAACCGACTTATAGAAAATGTAGTTGTCATATTAAGTGTCATTAAAATCAGTATTATGTGGAAATCAGTATTATTAGTCATTGTCAGGTTCGAAGTAAAGATAACCTAjuIPmlIBsaAIHindIIIExtracted region from T4 genomehomologous arm 4GAP2-T...mp-R H4-fwd...lamp 5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CATTATTTTTGTATATTCTGCTTTAAATGCATTCCTAAGCATAGATTCAGTAACTATATGCTCTTCATTAGAAAAATTATTTCTCAGAATATATCGTTTTATTTCAGGAATAGTTAATAGATGCTGTCCAGTTGAATATTCCATGTTTTTCCTCCATAGAGATTATACTCTAATAGTAATAAAAACATATAAGACGAAATTTACGTAAGGATTCGTATCTAAGTCATTGATATACGAGAAGTAATCTTTTTAATAAAGAGTCTTATATAGCAAAATAAAGTCCTTATCAATTATCTACGACAGGTCAACTTATAAGGTACAAAAAGGAGGTATCTCTAATATGAGATTATAjuIBsmINsiISapIBspQIBpu10IExtracted region from T4 genomehomologous arm 45,4405,4605,4805,5005,5205,5405,5605,5805,600
    AATTAAAGCATAATCTCTTATAAATTAAACCATTACAGTAAATCGACCAACTTTCTTCATTTGAAGATGCTGACCATATTCTTGCGGGTCATGGTCTTTATGCGAAATTATAAAAACGTTAGTGTTTTTCATTGAATTTATAATATTAGCTACACCTTTAATACCTTCGGCATCATTAATTTCGTATTAGAGAATATTTAATTTGGTAATGTCATTTAGCTGGTTGAAAGAAGTAAACTTCTACGACTGGTATAAGAACGCCCAGTACCAGAAATACGCTTTAATATTTTTGCAATCACAAAAAGTAACTTAAATATTATAATCGATGTGGAAATTATGGAAGCCGTAGTExtracted region from T4 genomehomologous arm 45,6205,6405,6605,6805,7005,7205,7405,760
    AATGACCCATCAAACACTTCATCAAGAATTAATGTACTAATACTAACACCAGATACGATAGAAGCAATATCACGCCAAGTAAATAAAAGAGCAATATCGATTCGTGCCTTTTCACCTTCACTAAATGAAGCATAACTAAAATCTTCACGACCACGGGATTTAATTGTCTCATTAATTACTGGGTAGTTTGTGAAGTAGTTCTTAATTACATGATTATGATTGTGGTCTATGCTATCTTCGTTATAGTGCGGTTCATTTATTTTCTCGTTATAGCTAAGCACGGAAAAGTGGAAGTGATTTACTTCGTATTGATTTTAGAAGTGCTGGTGCCCTAAATTAACAGAGTAATTBtgIClaIBspDIExtracted region from T4 genomehomologous arm 45,7805,8005,8205,8405,8605,8805,9005,9205,940
    ATTCTTCATCTAATGTAAACACATAATCCGCTTCCATTATTTTAAGATAATGGTTAATCTGCTTATTAAATAATGGAATGTACTTTTTAATAATAGCACCTTTAATACCAGAATCTTTGAGCATATCAGTCAAAATTCCTCGGTGGTATTTTTCCATTACTAAATTAGTTTTTGTTAAGAAGTAGATTACATTTGTGTATTAGGCGAAGGTAATAAAATTCTATTACCAATTAGACGAATAATTTATTACCTTACATGAAAAATTATTATCGTGGAAATTATGGTCTTAGAAACTCGTATAGTCAGTTTTAAGGAGCCACCATAAAAAGGTAATGATTTAATCAAAAACAExtracted region from T4 genomehomologous arm 45,9605,9806,0006,0206,0406,0606,0806,1006,120
    CTTAACAATTTTATCAAGTTCTTCTTGAAGCAGTGCTATTTCATCAGCATGGTCAATAAACTCAGAAGATGCTTTTTCTATAGCCGCTTTAACTTTTTTAGCTTTATCTACTGCTGCGATCAGAGATTGCTTTTTATTGCGAATATCACCAGAGGTTTTCACCGTCATCACCGAAGAATTGTTAAAATAGTTCAAGAAGAACTTCGTCACGATAAAGTAGTCGTACCAGTTATTTGAGTCTTCTACGAAAAAGATATCGGCGAAATTGAAAAAATCGAAATAGATGACGACGCTAGTCTCTAACGAAAAATAACGCTTATAGTGGTCTCCAAAAGTGGCAGTAGTGGCTTExtracted region from T4 genomehomologous arm 4Hom4-loc1-gib pUC-bkbone-Kpn1 6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ACGCGCGATGCGCGCTBsmBIEsp3I6,308
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6308 bp450050005500600050010001500GP45rep originBseYI, PspFIGsaIEcoP15Ibla geneDraIBauI, BssSI, Bst2BI,pUC19-fseq ,BspHI, CciI, PagI,AatII,ZraI,-35 signal,+14ApaLI, Alw44I, VneI,BauI, BssSI, Bst2BI,BpuEI,XmnI, Asp700I, MroXI, PdmI,+10BtsI,BtsI,PvuI, Ple19I,TaqII,PvuIBsrDI, Bse3DI, BseMI,AclI, Psp1406I,Acc16I, FspI, NsbI,BsrDI,FspIVspI, AseI, PshBI,EciI,NmeAIII,BglI,NmeAIII,BglIGsuI, BpmI,pUC-remBSA-F ,pUC-remBSA-R ,C->A removes BsaI site,+5Eam1105I, AhdI, BmeRI, DriI,AhdI,BanIDraI,DraI,BspHI, CciI, PagIBpuEI,-35 signal-10 signal,EcoP15I,RNAI transcriptEco57I, AcuI,RNAI promoter (clockwise); GCTACA -10 signal,+1AlwNI, CaiI, PstNI,EcoP15I,AlwNI,MmeIBpuEIApaLI, Alw44I, VneI,ApaLI,BaeGI,BseYIPvuII,XmnI, Asp700I, MroXI, PdmI,BbsI, BpiI, BstV2I,+3EcoP15IBalI,BalI, MlsI, MluNI, Mox20I, MscI, Msp20I,+8SspIPsiI, AanI,BsaBI, Bse8I, BseJI,MfeI, MunI,BsaBI,MfeIGAP2-Trans-clmp-R ,H4-fwd-GAP-clamp ,GAP2,AjuI,BsaAI,PmlIHindIII,HindIIIAjuIBsmI,NsiI,Bpu10I,BspQI,SapIExtracted region from T4 genome,homologous arm 4ClaI,BspDI,ClaI,BtgIHom4-loc1-gib ,pUC-bkbone-Kpn1 ,BsmBI,Esp3I

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    6308