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  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAZraIAatIIBciVIBsaHIEcoO109IpUC19-fseq 20406080100120140160
    TCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGAcuIApaLIBaeGIbla genebla signal peptide180200220240260280300320340
    AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAATCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTBcgIScaIXmnIBsaHITsp45Ibla geneBcgIXm...I360380400420440460480500520
    AGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGAEaeIPvuIbla gene540560580600620640660680700
    GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTCTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGABglIFspIBsrDITsp45INmeAIIIbla geneBglI720740760780800820840860
    GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCAAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATAhdIBanIBmrIBpmIBsrDIBsrFIbla genepUC-remBSA-F pUC-remBSA-R Ea...I8809009209409609801,0001,0201,040
    AGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCbl...e1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTATAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATAcuIBceAIrep originRNAI transcript1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCACCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTMmeIAlwNIApaLIBaeGIBseYIrep origin1,4201,4401,4601,4801,5001,5201,5401,560
    GCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAMmeIBciVIHaeIIrep origin1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGDrdINspIPciIAflIIIrep originDrd...DI1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCACGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTEaeISapIBsaXIBspQIHaeIIPvuIIseq verifiedBsaXI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCcagtaaagatgcgaaaaagattcctactgtcgattttgaccTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGgtcatttctacgctttttctaaggatgacagctaaaactggBanIBmrIseq verifiedH11.rev.T4genomeH11pUC19-rseqbk.donor.H11CAP...nd2,1202,1402,1602,1802,2002,2202,2402,260
    cacgcatcaaggcgataaaccatggaagcatgtatataataatagccaacacgcctgaaactaaaaccataaaaattgaacctgctactaaagtttccatgttttcctcacttaggtcaaattttttacacatgaattataagaattcactacatactccatcggagcgtttttagtgcgtagttccgctatttggtaccttcgtacatatattattatcggttgtgcggactttgattttggtatttttaacttggacgatgatttcaaaggtacaaaaggagtgaatccagtttaaaaaatgtgtacttaatattcttaagtgatgtatgaggtagcctcgcaaaaatBtgINcoINspIStyIBfuAIBspMIEcoNIEcoRI*126TL124NK122VC120SN118YS116NV114VY112EM110PA108NK1062387-2764*2K1H11seq verifiedimm.1 CDS (iduronate sulfatase)imm.1 geneseq verified2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cctgtacgccactggtaattattagcccaatttgcccaaagctcagcgcagtagttttcaattttttcttcgcgtgtaattacatctgaattacgatatgcttgagcagattcatctggacgaatagcttcgtcaaaattcgcctgcatttgttctactgtctgttttggagcttggacatgcggtgaccattaataatcgggttaaacgggtttcgagtcgcgtcatcaaaagttaaaaaagaagcgcacattaatgtagacttaatgctatacgaactcgtctaagtagacctgcttatcgaagcagttttaagcggacgtaaacaagatgacagacaaaacctcgaaBlpIXmnIGT104RW102QY100NN98AW96NA94WL92EA90CY88NE86IK84EE82RT80IV78DS76NR74YA72QA70SE68DP66RI64AE62DF60NA58QM56QE54VT52QK50PA48E2387-2764H11imm.1 CDS (iduronate sulfatase)imm.1 geneseq verified2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ctttataacacttgacattaggattataaaatttgcttgaacagtttacaatttttcctacatcagactgatttactaccggtccttgagctacacaaccagcaagacctaatgcaataaccaaaatagcgattttcatGTTTACCGGGAGGAAGATATAGCACTATCAGAAAGAgaaatattgtgaactgtaatcctaatattttaaacgaacttgtcaaatgttaaaaaggatgtagtctgactaaatgatggccaggaactcgatgtgttggtcgttctggattacgttattggttttatcgctaaaagtaCAAATGGCCCTCCTTCTATATCGTGATAGTCTTTCTAgeIBsrFIK46YC44KV42NP40NY38FK36SS34CN32VI30KG28VD26SQ24NV22VP20GQ18AV16CG14AL12GL10AI8VL6IA4IK2M2387-2764seq verifiedH11imm.1 CDS (iduronate sulfatase)imm.1 geneH11.fwd.T4genomebk.donor.H4GA... H4... ho...42,6402,6602,6802,7002,7202,7402,7602,7802,800
    GACAAAACCCATGCTCGACGAATTTTATTTTGGTTTTGTGGATTTGATTCAGAACGCACGTGTTTTGGCTGAATATCTTTTACATCAACAGTATAATTCACAGTAATTTTAGTCATAATACACCTTTAGTCATAATAATCAGTAACAGTCCAAGCTTCATTTCTATTGGACATTACTGTTTTGGGTACGAGCTGCTTAAAATAAAACCAAAACACCTAAACTAAGTCTTGCGTGCACAAAACCGACTTATAGAAAATGTAGTTGTCATATTAAGTGTCATTAAAATCAGTATTATGTGGAAATCAGTATTATTAGTCATTGTCAGGTTCGAAGTAAAGATAACCTGTAATAjuIPmlIBsaAIAflIIIHindIIIseq verifiedhomologous arm 4GAP...R H4-...p bk...42,8202,8402,8602,8802,9002,9202,9402,960
    TTTTTGTATATTCTGCTTTAAATGCATTCCTAAGCATAGATTCAGTAACTATATGCTCTTCATTAGAAAAATTATTTCTCAGAATATATCGTTTTATTTCAGGAATAGTTAATAGATGCTGTCCAGTTGAATATTCCATGTTTTTCCTCCATAGAGATTATACTCTAATAAATTAAAAAACATATAAGACGAAATTTACGTAAGGATTCGTATCTAAGTCATTGATATACGAGAAGTAATCTTTTTAATAAAGAGTCTTATATAGCAAAATAAAGTCCTTATCAATTATCTACGACAGGTCAACTTATAAGGTACAAAAAGGAGGTATCTCTAATATGAGATTATTTAATAjuIBsmINsiISapIBspQIBpu10Ihomologous arm 4seq verified2,9803,0003,0203,0403,0603,0803,1003,1203,140
    AAGCATAATCTCTTATAAATTAAACCATTACAGTAAATCGACCAACTTTCTTCATTTGAAGATGCTGACCATATTCTTGCGGGTCATGGTCTTTATGCGAAATTATAAAAACGTTAGTGTTTTTCATTGAATTTATAATATTAGCTACACCTTTAATACCTTCGGCATCAAATGATTCGTATTAGAGAATATTTAATTTGGTAATGTCATTTAGCTGGTTGAAAGAAGTAAACTTCTACGACTGGTATAAGAACGCCCAGTACCAGAAATACGCTTTAATATTTTTGCAATCACAAAAAGTAACTTAAATATTATAATCGATGTGGAAATTATGGAAGCCGTAGTTTACThomologous arm 43,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCCATCAAACACTTCATCAAGAATTAATGTACTAATACTAACACCAGATACGATAGAAGCAATATCACGCCAAGTAAATAAAAGAGCAATATCGATTCGTGCCTTTTCACCTTCACTAAATGAAGCATAACTAAAATCTTCACGACCACGGGATTTAATTGTCTCATTAAATTCTGGGTAGTTTGTGAAGTAGTTCTTAATTACATGATTATGATTGTGGTCTATGCTATCTTCGTTATAGTGCGGTTCATTTATTTTCTCGTTATAGCTAAGCACGGAAAAGTGGAAGTGATTTACTTCGTATTGATTTTAGAAGTGCTGGTGCCCTAAATTAACAGAGTAATTTAAGABtgIClaIBspDIhomologous arm 43,3403,3603,3803,4003,4203,4403,4603,4803,500
    TCATCTAATGTAAACACATAATCCGCTTCCATTATTTTAAGATAATGGTTAATCTGCTTATTAAATAATGGAATGTACTTTTTAATAATAGCACCTTTAATACCAGAATCTTTGAGCATATCAGTCAAAATTCCTCGGTGGTATTTTTCCATTACTAAATTAGTTTTTGTCTTAAAGTAGATTACATTTGTGTATTAGGCGAAGGTAATAAAATTCTATTACCAATTAGACGAATAATTTATTACCTTACATGAAAAATTATTATCGTGGAAATTATGGTCTTAGAAACTCGTATAGTCAGTTTTAAGGAGCCACCATAAAAAGGTAATGATTTAATCAAAAACAGAATThomologous arm 43,5203,5403,5603,5803,6003,6203,6403,660
    CAATTTTATCAAGTTCTTCTTGAAGCAGTGCTATTTCATCAGCATGGTCAATAAACTCAGAAGATGCTTTTTCTATAGCCGCTTTAACTTTTTTAGCTTTATCTACTGCTGCGATCAGAGATTGCTTTTTATTGCGAATATCACCAGAGGTTTTCACCGTCATCACCGAAACGCGGTTAAAATAGTTCAAGAAGAACTTCGTCACGATAAAGTAGTCGTACCAGTTATTTGAGTCTTCTACGAAAAAGATATCGGCGAAATTGAAAAAATCGAAATAGATGACGACGCTAGTCTCTAACGAAAAATAACGCTTATAGTGGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCBsmBIEsp3Ihomologous arm 4Hom4-loc1-gib pUC-bkbone-Kpn1 3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CGAGCT3,852
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    Editing disabled on read-only sequence.
    3853 bp2750300032503500250500750homologous arm 4bla geneseq verifiedBmrI, BmuIpUC-remBSA-F pUC-remBSA-R DraIClaI

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    3853