Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
Donor
  • Sequence Map
    Editing disabled on read-only sequence.
    GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCACTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTZraIAatIIBciVIBsaHIEcoO109IpUC19-fseq 20406080100120140160
    ATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCAAcuIApaLIBaeGIbla genebla signal peptide180200220240260280300320340
    AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATBcgIScaIXmnIBsaHITsp45Ibla geneBcgIXm...I360380400420440460480500520
    CGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTAEaeIPvuIbla gene540560580600620640660680700
    ACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTTGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGABglIFspIBsrDITsp45INmeAIIIbla geneBglI720740760780800820840860880
    GATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCTATTTAGACCTCGGCCACTCGCACCAAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCAhdIBanIBmrIBpmIBsrDIBsrFIbla genepUC-remBSA-F pUC-remBSA-R Ea...I9009209409609801,0001,0201,0401,060
    ACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAArep origin1,0801,1001,1201,1401,1601,1801,2001,2201,240
    TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATAcuIBceAIrep originRNAI transcript1,2601,2801,3001,3201,3401,3601,3801,4001,420
    GCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGMmeIAlwNIApaLIBaeGIBseYIrep origin1,4401,4601,4801,5001,5201,5401,5601,5801,600
    TGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCDrdIMmeIBciVIHaeIIrep originDrd...DI1,6201,6401,6601,6801,7001,7201,7401,7601,780
    ATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGNspIPciIAflIIIrep origin1,8001,8201,8401,8601,8801,9001,9201,940
    CGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGBanIEaeISapIBsaXIBspQIHaeIIPvuIIseq verifiedpUC19-rseqCAP...ndBsaXI1,9601,9802,0002,0202,0402,0602,0802,1002,120
    CCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCcagtaaagatgcgaaaaagattcctactgtcgattttgacccacgcatcaaggcgataaaccatggaagcatgtatataaGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGgtcatttctacgctttttctaaggatgacagctaaaactgggtgcgtagttccgctatttggtaccttcgtacatatattBmrIBtgINcoINspIStyIseq verifiedH11H11.rev.T4genomebk.donor.H112,1402,1602,1802,2002,2202,2402,2602,2802,300
    taatagccaacacgcctgaaactaaaaccataaaaattgaacctgctactaaagtttccatgttttcctcacttaggtcaaattttttacacatgaattataagaattcactacatactccatcggagcgtttttacctgtacgccactggtaattattagcccaatttgcccaaagcattatcggttgtgcggactttgattttggtatttttaacttggacgatgatttcaaaggtacaaaaggagtgaatccagtttaaaaaatgtgtacttaatattcttaagtgatgtatgaggtagcctcgcaaaaatggacatgcggtgaccattaataatcgggttaaacgggtttcgBfuAIBspMIEcoNIEcoRI*126TL124NK122VC120SN118YS116NV114VY112EM110PA108NK106GT104RW102QY100NN98AW96NA94WL922387-2764*2K1H11imm.1 CDS (iduronate sulfatase)imm.1 geneseq verifiedseq verified2,3202,3402,3602,3802,4002,4202,4402,4602,480
    tcagcgcagtagttttcaattttttcttcgcgtgtaattacatctgaattacgatatgcttgagcagattcatctggacgaatagcttcgtcaaaattcgcctgcatttgttctactgtctgttttggagcttctttataacacttgacattaggattataaaatttgcttgaacagtagtcgcgtcatcaaaagttaaaaaagaagcgcacattaatgtagacttaatgctatacgaactcgtctaagtagacctgcttatcgaagcagttttaagcggacgtaaacaagatgacagacaaaacctcgaagaaatattgtgaactgtaatcctaatattttaaacgaacttgtcaBlpIXmnIEA90CY88NE86IK84EE82RT80IV78DS76NR74YA72QA70SE68DP66RI64AE62DF60NA58QM56QE54VT52QK50PA48EK46YC44KV42NP40NY38FK36SS34CN322387-2764H11imm.1 CDS (iduronate sulfatase)imm.1 geneseq verified2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ttacaatttttcctacatcagactgatttactaccggtccttgagctacacaaccagcaagacctaatgcaataaccaaaatagcgattttcatGTTTACCGGGAGGAAGATATAGCACTATCAGAAAGAGACAAAACCCATGCTCGACGAATTTTATTTTGGTTTTGTGGATTTGATaatgttaaaaaggatgtagtctgactaaatgatggccaggaactcgatgtgttggtcgttctggattacgttattggttttatcgctaaaagtaCAAATGGCCCTCCTTCTATATCGTGATAGTCTTTCTCTGTTTTGGGTACGAGCTGCTTAAAATAAAACCAAAACACCTAAACTAAgeIAjuIBsrFIVI30KG28VD26SQ24NV22VP20GQ18AV16CG14AL12GL10AI8VL6IA4IK2M2387-2764seq verifiedH11imm.1 CDS (iduronate sulfatase)imm.1 genehomologous arm 4H11.fwd.T4genomebk.donor.H4GAP2-Trans-clmp-R H4-fwd-GAP-clamp 2,6802,7002,7202,7402,7602,7802,8002,8202,840
    TCAGAACGCACGTGTTTTGGCTGAATATCTTTTACATCAACAGTATAATTCACAGTAATTTTAGTCATAATACACCTTTAGTCATAATAATCAGTAACAGTCCAAGCTTCATTTCTATTGGACATTATTTTTGTATATTCTGCTTTAAATGCATTCCTAAGCATAGATTCAGTAACTAAGTCTTGCGTGCACAAAACCGACTTATAGAAAATGTAGTTGTCATATTAAGTGTCATTAAAATCAGTATTATGTGGAAATCAGTATTATTAGTCATTGTCAGGTTCGAAGTAAAGATAACCTGTAATAAAAACATATAAGACGAAATTTACGTAAGGATTCGTATCTAAGTCATTGATAjuIBsmINsiIPmlIBsaAIAflIIIBpu10IHindIIIseq verifiedhomologous arm 42,8602,8802,9002,9202,9402,9602,9803,0003,020
    TATGCTCTTCATTAGAAAAATTATTTCTCAGAATATATCGTTTTATTTCAGGAATAGTTAATAGATGCTGTCCAGTTGAATATTCCATGTTTTTCCTCCATAGAGATTATACTCTAATAAATTAAAGCATAATCTCTTATAAATTAAACCATTACAGTAAATCGACCAACTTTCTTCAATACGAGAAGTAATCTTTTTAATAAAGAGTCTTATATAGCAAAATAAAGTCCTTATCAATTATCTACGACAGGTCAACTTATAAGGTACAAAAAGGAGGTATCTCTAATATGAGATTATTTAATTTCGTATTAGAGAATATTTAATTTGGTAATGTCATTTAGCTGGTTGAAAGAAGTSapIBspQIhomologous arm 4seq verified3,0403,0603,0803,1003,1203,1403,1603,1803,200
    TTTGAAGATGCTGACCATATTCTTGCGGGTCATGGTCTTTATGCGAAATTATAAAAACGTTAGTGTTTTTCATTGAATTTATAATATTAGCTACACCTTTAATACCTTCGGCATCAAATGACCCATCAAACACTTCATCAAGAATTAATGTACTAATACTAACACCAGATACGATAGAAAACTTCTACGACTGGTATAAGAACGCCCAGTACCAGAAATACGCTTTAATATTTTTGCAATCACAAAAAGTAACTTAAATATTATAATCGATGTGGAAATTATGGAAGCCGTAGTTTACTGGGTAGTTTGTGAAGTAGTTCTTAATTACATGATTATGATTGTGGTCTATGCTATCThomologous arm 43,2203,2403,2603,2803,3003,3203,3403,3603,380
    AGCAATATCACGCCAAGTAAATAAAAGAGCAATATCGATTCGTGCCTTTTCACCTTCACTAAATGAAGCATAACTAAAATCTTCACGACCACGGGATTTAATTGTCTCATTAAATTCTTCATCTAATGTAAACACATAATCCGCTTCCATTATTTTAAGATAATGGTTAATCTGCTTATCGTTATAGTGCGGTTCATTTATTTTCTCGTTATAGCTAAGCACGGAAAAGTGGAAGTGATTTACTTCGTATTGATTTTAGAAGTGCTGGTGCCCTAAATTAACAGAGTAATTTAAGAAGTAGATTACATTTGTGTATTAGGCGAAGGTAATAAAATTCTATTACCAATTAGACGAATBtgIClaIBspDIhomologous arm 43,4003,4203,4403,4603,4803,5003,5203,5403,560
    TTAAATAATGGAATGTACTTTTTAATAATAGCACCTTTAATACCAGAATCTTTGAGCATATCAGTCAAAATTCCTCGGTGGTATTTTTCCATTACTAAATTAGTTTTTGTCTTAACAATTTTATCAAGTTCTTCTTGAAGCAGTGCTATTTCATCAGCATGGTCAATAAACTCAGAAGAATTTATTACCTTACATGAAAAATTATTATCGTGGAAATTATGGTCTTAGAAACTCGTATAGTCAGTTTTAAGGAGCCACCATAAAAAGGTAATGATTTAATCAAAAACAGAATTGTTAAAATAGTTCAAGAAGAACTTCGTCACGATAAAGTAGTCGTACCAGTTATTTGAGTCTTChomologous arm 43,5803,6003,6203,6403,6603,6803,7003,720
    ATGCTTTTTCTATAGCCGCTTTAACTTTTTTAGCTTTATCTACTGCTGCGATCAGAGATTGCTTTTTATTGCGAATATCACCAGAGGTTTTCACCGTCATCACCGAAACGCGCGATACGAAAAAGATATCGGCGAAATTGAAAAAATCGAAATAGATGACGACGCTAGTCTCTAACGAAAAATAACGCTTATAGTGGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTBsmBIEsp3Ihomologous arm 4Hom4-loc1-gib pUC-bkbone-Kpn1 3,7403,7603,7803,8003,8203,840
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    3853 bp2750300032503500250500750homologous arm 4bla geneseq verifiedBmrI, BmuIpUC-remBSA-F pUC-remBSA-R DraIClaIBauI, BssSI, Bst2BI,pUC19-fseq ,BspHI, CciI, PagI,AatII,ZraI,-35 signal,+10SspI,Bst6I, Eam1104I, EarI,bla signal peptideEco57I, AcuI,ApaLI, Alw44I, VneI,BauI, BssSI, Bst2BI,BpuEI,ApaLI,+2XmnI, Asp700I, MroXI, PdmI,AclI, Psp1406I,DraI,XmnI,BsaHIBcgI,TaqII,ScaI,ScaI, BmcAI, ZrmI,BcgI,ScaI,Tsp45IBtsIBtsIPvuI, Ple19I,TaqII,EaeI,PvuITsp45IBsrDI, Bse3DI, BseMI,AclI, Psp1406I,Acc16I, FspI, NsbI,+2VspI, AseI, PshBIEciI,NmeAIII,BglI,NmeAIII,BglIGsuI, BpmI,C->A removes BsaI site,BsrFIBsrDI, Bse3DI, BseMI,BpmI,BsrDI,BmrIHindIII,HindIII,AjuIBsmI,NsiI,Bpu10IBspQISapIBspDI,ClaIBtgIHom4-loc1-gib ,pUC-bkbone-Kpn1 ,BsmBI,Esp3I

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    3853