Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pAAV-EF1a-DIO hChR2 (E123T/T159C)-p2A-mCherry
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaagctttgcaaagatggataggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcgaaacgtttctacctatEagIMluINotIHindIIIL-ITREF-1a20406080100120140160
    aagttttaaacagagaggaatctttgcagctaatggaccttctaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaattcaaaatttgtctctccttagaaacgtcgattacctggaagatccagaactttcctcacccttaaccgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttggccacggatctcttccaccgcgccccattAgeIMfeIEcoNIEF-1a180200220240260280300320340
    actgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggccctgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtccattcacggcacacaccaagggcgcccggaccggagaaatgcccaataccgggEF-1a360380400420440460480500520
    ttgcgtgccttgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccaacgcacggaacttaatgaaggtgaccgacgtcatgcactaagaactagggctcgaagcccaaccttcacccaccctctcaagctccggaacgcgaattcctcggggaagcggagcacgaactcaactccggaccggacccgcgaccccggcggcgcacgcttagaccaccgtggAflIIEF-1a540560580600620640660680700
    ttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacaagcgcggacagagcgacgaaagctattcagagatcggtaaattttaaaaactactggacgacgctgcgaaaaaaagaccgttctatcagaacatttacgcccggttctagacgtgtgaccataaagccaaaaaccccggcgcccgccgctgccccgggcacgcagggtcgcgtgApaIPciIBfuAIBspMIPspOMIEF-1a720740760780800820840860
    atgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttccctacaagccgctccgccccggacgctcgcgccggtggctcttagcctgcccccatcagagttcgaccggccggacgagaccacggaccggagcgcggcggcacatagcggggcgggacccgccgttccgaccgggccagccgtggtcaacgcactcgcctttctaccggcgaagggFseIPasIBstAPIEF-1a8809009209409609801,0001,0201,040
    ggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtccgggacgacgtccctcgagttttacctcctgcgccgcgagccctctcgcccgcccactcagtgggtgtgtttccttttcccggaaaggcaggagtcggcagcgaagtacactgaggtgcctcatggcccgcggcaggtccgtggagctaatcaagagctcgaaaacctcatgcaSacIEcoNIEco53kIEF-1a1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttctgcagaaatccaacccccctccccaaaatacgctacctcaaaggggtgtgactcacccacctctgacttcaatccggtcgaaccgtgaactacattaagaggaaccttaaacgggaaaaactcaaacctagaaccaagtaagagttcggagtctgtcaccaagtttcaaaaaaagaEF-1a1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tccatttcaggtgtcgtgaggtaccggatcctctagagtcgactccggaataacttcgtataggatactttatacgaagttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgccaggtaaagtccacagcactccatggcctaggagatctcagctgaggccttattgaagcatatcctatgaaatatgcttcaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattattgaagcatatcgtatgtaatatgcttcaataccgcgcggAccIAscIKpnISalIXbaIBamHIBspEIAcc65IHincIIlox2722loxPEF-1a1,4201,4401,4601,4801,5001,5201,5401,560
    ttacttgtacagctcgtccatgccgccggtggagtggcggccctcggcgcgttcgtactgttccacgatggtgtagtcctcgttgtgggaggtgatgtccaacttgatgttgacgttgtaggcgccgggcagctgcacgggcttcttggccttgtaggtggtcttgacctcagcgaatgaacatgtcgagcaggtacggcggccacctcaccgccgggagccgcgcaagcatgacaaggtgctaccacatcaggagcaacaccctccactacaggttgaactacaactgcaacatccgcggcccgtcgacgtgcccgaagaaccggaacatccaccagaactggagtcgcAleIXcmIBsrGISgrAIHincIImCherry1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcgtagtggccgccgtccttcagcttcagcctctgcttgatctcgcccttcagggcgccgtcctcggggtacatccgctcggaggaggcctcccagcccatggtcttcttctgcattacggggccgtcggaggggaagttggtgccgcgcagcttcaccttgtagatgaactcgcagcatcaccggcggcaggaagtcgaagtcggagacgaactagagcgggaagtcccgcggcaggagccccatgtaggcgagcctcctccggagggtcgggtaccagaagaagacgtaatgccccggcagcctccccttcaaccacggcgcgtcgaagtggaacatctacttgagcgPflMImCherry1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cgtcctgcagggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcaagtagtcggggatgtcggcggggtgcttcacgtaggccttggagccgtacatgaactgaggggacaggatgtcccagcaggacgtccctcctcaggacccagtgccagtggtgcggcggcaggagcttcaagtagtgcgcgagggtgaacttcgggagccccttcctgtcgaagttcatcagcccctacagccgccccacgaagtgcatccggaacctcggcatgtacttgactcccctgtcctacagggtmCherry1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggcgaagggcagggggccacccttggtcaccttcagcttggcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcacggagccctccatgtgcaccttgaagcgcatgaactccttgatgatggccatgttatcctcctcgcccccgcttcccgtcccccggtgggaaccagtggaagtcgaaccgccagacccacgggagcatccccgccgggagcgggagcgggagctagagcttgagcaccggcaagtgcctcgggaggtacacgtggaacttcgcgtacttgaggaactactaccggtacaataggaggagcgggmCherry2,1202,1402,1602,1802,2002,2202,2402,260
    ttgctcacgggaccggggttttcttccacgtctcctgcttgctttaacagagagaagttcgtggctccggagccgggcgcgcctggcacggctccggcctcggcttcgtcttcgacgagagtctcgacctcgatctccgttccgccgatgttcagtttggtggttttgcggatataacgagtgccctggccccaaaagaaggtgcagaggacgaacgaaattgtctctcttcaagcaccgaggcctcggcccgcgcggaccgtgccgaggccggagccgaagcagaagctgctctcagagctggagctagaggcaaggcggctacaagtcaaaccaccaaaacgcctataAscIBmgBIBspEIEcoRVPshAIhChR2p2A2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctccgtgaatcaatatgtgctcgtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttactcatcagatcaataatcgtgtgacctacggtggagccatagacgctcaggacgccaaaaccttcgggccccaaaatgaagagaattgggaacataccccagctgaggcacttagttatacacgagcacctagtcctgcgcgtccatcacagggttgttgggggttgttaaaaatgagtagtctagttattagcacactggatgccacctcggtatctgcgagtcctgcggttttggaagcccggggttttacttctcttaacccttgtatggggtcgaApaIBfuAIBspMIPspOMIhChR22,4602,4802,5002,5202,5402,5602,5802,6002,620
    cacgaaaaacagccatgccatgccggtcacgacctggcggcaccgaccctttggcacagtatgataaccctcgatatatgctttggcggcgtgaaaaaatgtgttcgcgccatagcacaatccaagacaaaagaagatgactttaacatagccggttgccatggcgctggtagccgtgctttttgtcggtacggtacggccagtgctggaccgccgtggctgggaaaccgtgtcatactattgggagctatatacgaaaccgccgcacttttttacacaagcgcggtatcgtgttaggttctgttttcttctactgaaattgtatcggccaacggtaccgcgaccatcgghChR22,6402,6602,6802,7002,7202,7402,7602,7802,800
    ccccacacgatacacccgatgtctgagacaaggagtcccatggttctcctgctgtagtcgttgctcaggccggtgaggttgctcaggtggataaggatgacaggacaagtgagcagccaggttgcatagcgcagccactgcacccggtgtcctgtggcaaggtagagcatagaggggggtgtgctatgtgggctacagactctgttcctcagggtaccaagaggacgacatcagcaacgagtccggccactccaacgagtccacctattcctactgtcctgttcactcgtcggtccaacgtatcgcgtcggtgacgtgggccacaggacaccgttccatctcgtatctccBstAPIhChR22,8202,8402,8602,8802,9002,9202,9402,960
    gattcttaaactcaaaaaagaactcgagaatcaccttaaccatttcaatggcgcacacatagatctcctcccagccgcatgtagatttccaggtttggtaggcatagaacatcagcagcaaaatgctgaatcctgctgcaagccactgcaggacatttgacgcggtctgagcgccctaagaatttgagttttttcttgagctcttagtggaattggtaaagttaccgcgtgtgtatctagaggagggtcggcgtacatctaaaggtccaaaccatccgtatcttgtagtcgtcgttttacgacttaggacgacgttcggtgacgtcctgtaaactgcgccagactcgcggPflFITth111IhChR22,9803,0003,0203,0403,0603,0803,1003,1203,140
    gttcgtgccgcgagattcaatccatccggcacagtaacattgatcctcagggaccaggacggacccgttcaccaccacaggattagtaacgaacaaaagttcgcgtccgacggcagacaaagcgccgccatagtccatggtggctagcataacttcgtataaagtatcctatacgcaagcacggcgctctaagttaggtaggccgtgtcattgtaactaggagtccctggtcctgcctgggcaagtggtggtgtcctaatcattgcttgttttcaagcgcaggctgccgtctgtttcgcggcggtatcaggtaccaccgatcgtattgaagcatatttcataggatatgcBmtINheIBsu36IhChR2lox27223,1603,1803,2003,2203,2403,2603,2803,3003,320
    aagttatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctttcaataaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattacatacgatatgcttcaatacttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaClaIBspDIEcoRIEcoRVHindIIIWPREloxP3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggWPRE3,5203,5403,5603,5803,6003,6203,6403,660
    ccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggPfoIPflMIWPRE3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccaaccgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggZraIAatIIWPRE3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtgggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacAfeIAleIClaIBspDICspCIhGH polyAWPRE4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcactccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtBcgIhGH polyA4,2204,2404,2604,2804,3004,3204,3404,360
    tgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccBlpIBsaINsiISphIhGH polyA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    taaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggacEagINotIPmlIRsrIIR-ITR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    atgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttctacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaagf1 Origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccgaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggf1 Origin4,9204,9404,9604,9805,0005,0205,0405,060
    acgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttattgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataPsiIf1 Origin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggcttPfoI5,2605,2805,3005,3205,3405,3605,3805,4005,420
    acgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgatgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactZraIAatII5,4405,4605,4805,5005,5205,5405,5605,5805,600
    taaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgaccAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    atctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagttagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcaBcgIScaIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttPvuIAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggAseIFspIAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagBsaIAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttcAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    atcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcgpUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacgcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    acagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttattgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatapUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    agtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIpUC ori7,1807,2007,2207,2407,2607,2807,300
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7314 bp5000550060006500700050010001500mCherryL-ITR,EagI,NotI,MluI,HindIII,MfeI,AgeI,EcoNIAflIIEF-1a,BfuAI,BspMI,PspOMI,ApaI,PciIFseIPasIBstAPI,EcoNI,Eco53kI,SacIlox2722,loxP,Acc65I,KpnI,BamHI,XbaI,SalI,AccI,HincII,BspEIAscIBsrGISgrAI,XcmI,AleI,HincIIZraIAatIIBcgI,ScaIAmpR,PvuI,FspIAseIBsaIpUC ori,PciI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    7314