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peSpCas9-J23109
  • Sequence Map
    Editing disabled on read-only sequence.
    ATGGATAAGAAATACTCAATAGGCTTAGaTATCGGCACAAATAGCGTCGGATGGGCGGTGATCACTGATGAATATAAGGTTCCGTCTAAAAAGTTCAAGGTTCTGGGAAATACAGACCGCCACAGTATCAAAAAAAATCTTATAGGGGCTCTTTTATTTGACAGTGGAGAGACAGTACCTATTCTTTATGAGTTATCCGAATCtATAGCCGTGTTTATCGCAGCCTACCCGCCACTAGTGACTACTTATATTCCAAGGCAGATTTTTCAAGTTCCAAGACCCTTTATGTCTGGCGGTGTCATAGTTTTTTTTAGAATATCCCCGAGAAAATAAACTGTCACCTCTCTGTCBclIHgaIeSpCas91.020406080100120140160
    CGGAAGCGACTCGTCTCAAACGGACAGCTCGTAGAAGGTATACACGTCGGAAGAATCGTATTTGTTATCTACAGGAGATTTTTTCAAATGAGATGGCGAAAGTAGATGATAGTTTCTTTCATCGACTTGAAGAGTCTTTTTTGGTGGAAGAAGACAAGAAGCATGAACGTCATCCGCCTTCGCTGAGCAGAGTTTGCCTGTCGAGCATCTTCCATATGTGCAGCCTTCTTAGCATAAACAATAGATGTCCTCTAAAAAAGTTTACTCTACCGCTTTCATCTACTATCAAAGAAAGTAGCTGAACTTCTCAGAAAAAACCACCTTCTTCTGTTCTTCGTACTTGCAGTAGGAccISfcIBmgBIBsmBIEsp3IBstZ17IeSpCas91.0180200220240260280300320340
    TATTTTTGGAAATATAGTAGATGAAGTTGCTTATCATGAGAAATATCCAACTATCTATCATCTGCGAAAAAAATTGGTAGATTCTACTGATAAAGCGGATTTGCGCTTAATCTATTTGGCCTTAGCGCATATGATTAAGTTTCGTGGTCATTTTTTGATTGAGGGAGATTTAAATATAAAAACCTTTATATCATCTACTTCAACGAATAGTACTCTTTATAGGTTGATAGATAGTAGACGCTTTTTTTAACCATCTAAGATGACTATTTCGCCTAAACGCGAATTAGATAAACCGGAATCGCGTATACTAATTCAAAGCACCAGTAAAAAACTAACTCCCTCTAAATTTANdeISwaIeSpCas91.0360380400420440460480500520
    CCTGATAATAGTGATGTGGACAAACTATTTATCCAGTTGGTACAAACCTACAATCAATTATTTGAAGAAAACCCTATTAACGCAAGTGGAGTAGATGCTAAAGCGATTCTTTCTGCACGATTGAGTAAATCAAGACGATTAGAAAATCTCATTGCTCAGCTCCCCGGTGAGAAGAGGACTATTATCACTACACCTGTTTGATAAATAGGTCAACCATGTTTGGATGTTAGTTAATAAACTTCTTTTGGGATAATTGCGTTCACCTCATCTACGATTTCGCTAAGAAAGACGTGCTAACTCATTTAGTTCTGCTAATCTTTTAGAGTAACGAGTCGAGGGGCCACTCTTCTBlpIBsgIeSpCas91.0540560580600620640660680700
    AAAATGGCTTATTTGGGAATCTCATTGCTTTGTCATTGGGTTTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAAGATGCTAAATTACAGCTTTCAAAAGATACTTACGATGATGATTTAGATAATTTATTGGCGCAAATTGGAGATCAATATGCTGATTTGTTTTTGGCTTTTACCGAATAAACCCTTAGAGTAACGAAACAGTAACCCAAACTGGGGATTAAAATTTAGTTTAAAACTAAACCGTCTTCTACGATTTAATGTCGAAAGTTTTCTATGAATGCTACTACTAAATCTATTAAATAACCGCGTTTAACCTCTAGTTATACGACTAAACAAAAACCGBstAPIeSpCas91.0720740760780800820840860
    AGCTAAGAATTTATCAGATGCTATTTTACTTTCAGATATCCTAAGAGTAAATACTGAAATAACTAAGGCTCCCCTATCAGCTTCAATGATTAAACGCTACGATGAACATCATCAAGACTTGACTCTTTTAAAAGCTTTAGTTCGACAACAACTTCCAGAAAAGTATAAAGAAATCTCGATTCTTAAATAGTCTACGATAAAATGAAAGTCTATAGGATTCTCATTTATGACTTTATTGATTCCGAGGGGATAGTCGAAGTTACTAATTTGCGATGCTACTTGTAGTAGTTCTGAACTGAGAAAATTTTCGAAATCAAGCTGTTGTTGAAGGTCTTTTCATATTTCTTTAGBsaXIeSpCas91.08809009209409609801,0001,0201,040
    TTTTTTGATCAATCAAAAAACGGATATGCAGGTTATATTGATGGGGGAGCTAGCCAAGAAGAATTTTATAAATTTATCAAACCAATTTTAGAAAAAATGGATGGTACTGAGGAATTATTGGTGAAACTAAATCGTGAAGATTTGCTGCGCAAGCAACGGACCTTTGACAACGGCTAAAAAACTAGTTAGTTTTTTGCCTATACGTCCAATATAACTACCCCCTCGATCGGTTCTTCTTAAAATATTTAAATAGTTTGGTTAAAATCTTTTTTACCTACCATGACTCCTTAATAACCACTTTGATTTAGCACTTCTAAACGACGCGTTCGTTGCCTGGAAACTGTTGCCGABclIFspIBfuAIBspMIeSpCas91.01,0601,0801,1001,1201,1401,1601,1801,2001,220
    CTATTCCCCATCAAATTCACTTGGGTGAGCTGCATGCTATTTTGAGAAGACAAGAAGACTTTTATCCATTTTTAAAAGACAATCGTGAGAAGATTGAAAAAATCTTGACTTTTCGAATTCCTTATTATGTTGGTCCATTGGCGCGTGGCAATAGTCGTTTTGCATGGATGACTCGGATAAGGGGTAGTTTAAGTGAACCCACTCGACGTACGATAAAACTCTTCTGTTCTTCTGAAAATAGGTAAAAATTTTCTGTTAGCACTCTTCTAACTTTTTTAGAACTGAAAAGCTTAAGGAATAATACAACCAGGTAACCGCGCACCGTTATCAGCAAAACGTACCTACTGAGCAleISphIBstBIEcoRIeSpCas91.01,2401,2601,2801,3001,3201,3401,3601,3801,400
    GAAGTCTGAAGAAACAATTACCCCATGGAATTTTGAAGAAGTTGTCGATAAAGGTGCTTCAGCTCAATCATTTATTGAACGCATGACAAACTTTGATAAAAATCTTCCAAATGAAAAAGTACTACCAAAACATAGTTTGCTTTATGAGTATTTTACGGTTTATAACGAATTGACACTTCAGACTTCTTTGTTAATGGGGTACCTTAAAACTTCTTCAACAGCTATTTCCACGAAGTCGAGTTAGTAAATAACTTGCGTACTGTTTGAAACTATTTTTAGAAGGTTTACTTTTTCATGATGGTTTTGTATCAAACGAAATACTCATAAAATGCCAAATATTGCTTAACTGTBcgIBtgIDrdINcoIScaITatIeSpCas91.01,4201,4401,4601,4801,5001,5201,5401,560
    AAGGTCAAATATGTTACTGAAGGAATGCGAAAACCAGCATTTCTTTCAGGTGAACAGAAGAAAGCCATTGTTGATTTACTCTTCAAAACAAATCGAAAAGTAACCGTTAAGCAATTAAAAGAAGATTATTTCAAAAAAATAGAATGTTTTGATAGTGTTGAAATTTCAGGAGTTGTTCCAGTTTATACAATGACTTCCTTACGCTTTTGGTCGTAAAGAAAGTCCACTTGTCTTCTTTCGGTAACAACTAAATGAGAAGTTTTGTTTAGCTTTTCATTGGCAATTCGTTAATTTTCTTCTAATAAAGTTTTTTTATCTTACAAAACTATCACAACTTTAAAGTCCTCAACPshAIeSpCas91.01,5801,6001,6201,6401,6601,6801,7001,7201,740
    AAGATAGATTTAATGCTTCATTAGGTACCTACCATGATTTGCTAAAAATTATTAAAGATAAAGATTTTTTGGATAATGAAGAAAATGAAGATATCTTAGAGGATATTGTTTTAACATTGACCTTATTTGAAGATAGGGAGATGATTGAGGAAAGACTTAAAACATATGCTCACCTTTCTATCTAAATTACGAAGTAATCCATGGATGGTACTAAACGATTTTTAATAATTTCTATTTCTAAAAAACCTATTACTTCTTTTACTTCTATAGAATCTCCTATAACAAAATTGTAACTGGAATAAACTTCTATCCCTCTACTAACTCCTTTCTGAATTTTGTATACGAGTGGABanIKpnINdeIAcc65IeSpCas91.01,7601,7801,8001,8201,8401,8601,8801,9001,920
    CTTTGATGATAAGGTGATGAAACAGCTTAAACGTCGCCGTTATACTGGTTGGGGACGTTTGTCTCGAAAATTGATTAATGGTATTAGGGATAAGCAATCTGGCAAAACAATATTAGATTTTTTGAAATCAGATGGTTTTGCCAATCGCAATTTTATGCAGCTGATCCATGATGATGAAACTACTATTCCACTACTTTGTCGAATTTGCAGCGGCAATATGACCAACCCCTGCAAACAGAGCTTTTAACTAATTACCATAATCCCTATTCGTTAGACCGTTTTGTTATAATCTAAAAAACTTTAGTCTACCAAAACGGTTAGCGTTAAAATACGTCGACTAGGTACTACTABsmFIPshAIPvuIIeSpCas91.01,9401,9601,9802,0002,0202,0402,0602,0802,100
    AGTTTGACATTTAAAGAAGACATTCAAAAAGCACAAGTGTCTGGACAAGGCGATAGTTTACATGAACATATTGCAAATTTAGCTGGTAGCCCTGCTATTAAAAAAGGTATTTTACAGACTGTAAAAGTTGTTGATGAATTGGTCAAAGTAATGGGGCGGCATAAGCCAGAAAATATCAAACTGTAAATTTCTTCTGTAAGTTTTTCGTGTTCACAGACCTGTTCCGCTATCAAATGTACTTGTATAACGTTTAAATCGACCATCGGGACGATAATTTTTTCCATAAAATGTCTGACATTTTCAACAACTACTTAACCAGTTTCATTACCCCGCCGTATTCGGTCTTTTATXmnIeSpCas91.02,1202,1402,1602,1802,2002,2202,2402,260
    TCGTTATTGAAATGGCACGTGAAAATCAGACAACTCAAAAGGGCCAGAAAAATTCGCGAGAGCGTATGAAACGAATCGAAGAAGGTATCAAAGAATTAGGAAGTCAGATTCTTAAAGAGCATCCTGTTGAAAATACTCAATTGCAAAATGAAGCACTCTATCTCTATTATCTCCAAGCAATAACTTTACCGTGCACTTTTAGTCTGTTGAGTTTTCCCGGTCTTTTTAAGCGCTCTCGCATACTTTGCTTAGCTTCTTCCATAGTTTCTTAATCCTTCAGTCTAAGAATTTCTCGTAGGACAACTTTTATGAGTTAACGTTTTACTTCGTGAGATAGAGATAATAGAGGTNruIPmlIeSpCas91.02,2802,3002,3202,3402,3602,3802,4002,4202,440
    AAATGGAAGAGACATGTATGTGGACCAAGAATTAGATATTAATCGTTTAAGTGATTATGATGTCGATcaCATTGTTCCACAAAGTTTCCTTAAAGACGATTCAATAGACAATAAGGTCTTAACGCGTTCTGATAAAAATCGTGGTAAATCGGATAACGTTCCAAGTGAAGAAGTATTTACCTTCTCTGTACATACACCTGGTTCTTAATCTATAATTAGCAAATTCACTAATACTACAGCTAgtGTAACAAGGTGTTTCAAAGGAATTTCTGCTAAGTTATCTGTTATTCCAGAATTGCGCAAGACTATTTTTAGCACCATTTAGCCTATTGCAAGGTTCACTTCTTCATAclIDrdIMluIPciIeSpCas91.02,4602,4802,5002,5202,5402,5602,5802,6002,620
    GTCAAAAAGATGAAAAACTATTGGAGACAACTTCTAAACGCCAAGTTAATCACTCAACGTAAGTTTGATAATTTAACGAAAGCTGAACGTGGAGGTTTGAGTGAACTTGATAAAGCTGGTTTTATCAAACGCCAATTGGTTGAAACTCGCCAAATCACTAAGCATGTGGCACAAACAGTTTTTCTACTTTTTGATAACCTCTGTTGAAGATTTGCGGTTCAATTAGTGAGTTGCATTCAAACTATTAAATTGCTTTCGACTTGCACCTCCAAACTCACTTGAACTATTTCGACCAAAATAGTTTGCGGTTAACCAACTTTGAGCGGTTTAGTGATTCGTACACCGTGTTTeSpCas91.02,6402,6602,6802,7002,7202,7402,7602,7802,800
    TTTTGGATAGTCGCATGAATACTAAATACGATGAAAATGATAAACTTATTCGAGAGGTTAAAGTGATTACCTTAAAATCTAAATTAGTTTCTGACTTCCGAAAAGATTTCCAATTCTATAAAGTACGTGAGATTAACAATTACCATCATGCCCATGATGCGTATCTAAATGCCGTAAAACCTATCAGCGTACTTATGATTTATGCTACTTTTACTATTTGAATAAGCTCTCCAATTTCACTAATGGAATTTTAGATTTAATCAAAGACTGAAGGCTTTTCTAAAGGTTAAGATATTTCATGCACTCTAATTGTTAATGGTAGTACGGGTACTACGCATAGATTTACGGCAeSpCas91.02,8202,8402,8602,8802,9002,9202,9402,960
    CGTTGGAACTGCTTTGATTAAGAAATATCCAGCACTTGAATCGGAGTTTGTCTATGGTGATTATAAAGTTTATGATGTTCGTAAAATGATTGCTAAGTCTGAGCAAGAAATAGGCAAAGCAACCGCAAAATATTTCTTTTACTCTAATATCATGAACTTCTTCAAAACAGAAATTGCAACCTTGACGAAACTAATTCTTTATAGGTCGTGAACTTAGCCTCAAACAGATACCACTAATATTTCAAATACTACAAGCATTTTACTAACGATTCAGACTCGTTCTTTATCCGTTTCGTTGGCGTTTTATAAAGAAAATGAGATTATAGTACTTGAAGAAGTTTTGTCTTTAABcgIXmnIeSpCas91.02,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACACTTGCAAATGGAGAGATTCGCAAAGCACCTCTAATCGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGCGAGATTTTGCCACAGTGCGCAAAGTATTGTCCATGCCCCAAGTCAATATTGTCAAGAAAACAGAAGTACAGACAGGCGGATTCTCCAAGGAGTTGTGAACGTTTACCTCTCTAAGCGTTTCGTGGAGATTAGCTTTGATTACCCCTTTGACCTCTTTAACAGACCCTATTTCCCGCTCTAAAACGGTGTCACGCGTTTCATAACAGGTACGGGGTTCAGTTATAACAGTTCTTTTGTCTTCATGTCTGTCCGCCTAAGAGGTTCCTCABpmIFspITatIBsaXIeSpCas91.03,1603,1803,2003,2203,2403,2603,2803,3003,320
    CAATTTTACCAAAAAGAAATTCGGACAAGCTTATTGCTCGTAAAAAAGACTGGGATCCAAAAAAATATGGTGGTTTTGATAGTCCAACGGTAGCTTATTCAGTCCTAGTGGTTGCTAAGGTGGAAAAAGGGAAATCGAAGAAGTTAAAATCCGTTAAAGAGTTACTAGGGATCACGTTAAAATGGTTTTTCTTTAAGCCTGTTCGAATAACGAGCATTTTTTCTGACCCTAGGTTTTTTTATACCACCAAAACTATCAGGTTGCCATCGAATAAGTCAGGATCACCAACGATTCCACCTTTTTCCCTTTAGCTTCTTCAATTTTAGGCAATTTCTCAATGATCCCTAGTGAjuIBmrIBamHIeSpCas91.03,3403,3603,3803,4003,4203,4403,4603,4803,500
    AATTATGGAAAGAAGTTCCTTTGAAAAAAATCCGATTGACTTTTTAGAAGCTAAAGGATATAAGGAAGTTAAAAAAGACTTAATCATTAAACTACCTAAATATAGTCTTTTTGAGTTAGAAAACGGTCGTAAACGGATGCTGGCTAGTGCCGGAGAATTACAAAAAGGAAATGAGTTAATACCTTTCTTCAAGGAAACTTTTTTTAGGCTAACTGAAAAATCTTCGATTTCCTATATTCCTTCAATTTTTTCTGAATTAGTAATTTGATGGATTTATATCAGAAAAACTCAATCTTTTGCCAGCATTTGCCTACGACCGATCACGGCCTCTTAATGTTTTTCCTTTACTCeSpCas91.03,5203,5403,5603,5803,6003,6203,6403,660
    CTGGCTCTGCCAAGCAAATATGTGAATTTTTTATATTTAGCTAGTCATTATGAAAAGTTGAAGGGTAGTCCAGAAGATAACGAACAAAAACAATTGTTTGTGGAGCAGCATAAGCATTATTTAGATGAGATTATTGAGCAAATCAGTGAATTTTCTAAGCGTGTTATTTTAGCAGGACCGAGACGGTTCGTTTATACACTTAAAAAATATAAATCGATCAGTAATACTTTTCAACTTCCCATCAGGTCTTCTATTGCTTGTTTTTGTTAACAAACACCTCGTCGTATTCGTAATAAATCTACTCTAATAACTCGTTTAGTCACTTAAAAGATTCGCACAATAAAATCGTCeSpCas91.03,6803,7003,7203,7403,7603,7803,8003,8203,840
    ATGCCAATTTAGATAAAGTTCTTAGTGCATATAACAAACATAGAGACAAACCAATACGTGAACAAGCAGAAAATATTATTCATTTATTTACGTTGACGAATCTTGGAGCTCCCGCTGCTTTTAAATATTTTGATACAACAATTGATCGTAAACGATATACGTCTACAAAAGAAGTTACGGTTAAATCTATTTCAAGAATCACGTATATTGTTTGTATCTCTGTTTGGTTATGCACTTGTTCGTCTTTTATAATAAGTAAATAAATGCAACTGCTTAGAACCTCGAGGGCGACGAAAATTTATAAAACTATGTTGTTAACTAGCATTTGCTATATGCAGATGTTTTCTTCAAccIFauISacIHincIIBsiHKAIEco53kIeSpCas91.03,8603,8803,9003,9203,9403,9603,9804,0004,020
    TTTAGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTATGAAACACGCATTGATTTGAGTCAGCTAGGAGGTGACTAACtcgagtaaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctcAAATCTACGGTGAGAATAGGTAGTTAGGTAGTGACCAGAAATACTTTGTGCGTAACTAAACTCAGTCGATCCTCCACTGATTGagctcattcctagaggtccgtagtttattttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagBmrIBpmIPsrITliIXhoIPspXIPaeR7IeSpCas91.0dblTerm4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacctagggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagatgatctcagtgtgaccgagtggaagcccacccggaaagacgcaaatatggatccctatataaggcgaaggagcgagtgactgagcgatgcgagccagcaagctgacgccgctcgcctttaccgaatgcttgccccgcctctaaaggaccttctacggtccttctatgaattgPfoIAvrIIBsrBIp15A OrigindblTerm4,2204,2404,2604,2804,3004,3204,3404,360
    agggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttcccttcactctcccggcgccgtttcggcaaaaaggtatccgaggcggggggactgttcgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccgccgagggagcacgcgagaggacaaggacggaaagccaaBtgIHgaIBssSISacIIBssSαIp15A Origin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    taccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaatggccacagtaaggcgacaataccggcgcaaacagagtaaggtgcggactgtgagtcaaggcccatccgtcaagcgaggttcgacctgacatacgtgcttggggggcaagtcaggctggcgacgcggaataggccattgatagcagaactcaggttgggcctttctgtacgtttAgeIEaeIBpuEIp15A Origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggctcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatcagaacttcagtacgcggccaattccgatttgactttcctgttcaaaaccactgacgcgaggaggttcggtcaatggagccaagtttctcaaccatcgagtctcttggaagctttttggcgggacgttccgAlwNIBstBIEcoP15Ip15A Origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagatttcagtgcaatttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgttactagtgcttggattctcaccaccaaaaaagcaaaagtctcgttctctaatgcgcgtctggttttgctagagttcttctagtagaataattagtctattttataaagatctaaagtcacgttaaatagagaagtttacatcgtggacttcagtcggggtatgctatattcaacaatgatcacgaacctaagagtggtSpeIXbaIBpuEIp15A Origin4,9204,9404,9604,9805,0005,0205,0405,060
    ataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatattattttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaataatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataSacIBsiHKAIEco53kIKanR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacPflMIKanR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatttaggccactcttaccgttttcgaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttaAjuINsiIPvuIAsiSIBsmBIEsp3IKanR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcgcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagBtsIBtsINsiISmaIXmaIBtsαIBtsαIEcoNITspMIKanR5,6205,6405,6605,6805,7005,7205,7405,760
    agccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccClaINruIBspDIKanR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aatttaatcgcggcctcgagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttccctgcagTTTACAGCttaaattagcgccggagctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactactatataaaaatagaacacgttacattgtagtctctaaaactctgtgttgcaccgaaagggacgtcAAATGTCGPstISfcITliIXhoIPspXIDraIIIPaeR7IKanR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TAGCTCAGTCCTAGGGACTGTGCTAGCggccgcagatctaaagaggagaaaggatctATCGAGTCAGGATCCCTGACACGATCGccggcgtctagatttctcctctttcctagaEaeIEagINotIAvrIIBglIIBsmFIpot...BS6,1406,1606,180
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6182 bp45005000550050010001500eSpCas91.0TSSHgaI,BclI,BsmBI,Esp3I,AccI,BstZ17I,BmgBI,SfcINdeI,SwaIBsgI,BlpI,BstAPIBsaXIBclIBfuAI,BspMIFspI,AleI,SphIBstBI,EcoRI,DrdI,BtgI,NcoIBcgITatI,ScaIBpuEI,AlwNIEcoP15IBstBI,BpuEI,XbaISpeIEco53kI,BsiHKAI,SacIPflMINsiI,AjuI,BsmBI,Esp3I,AsiSI,PvuIKanR,BtsI,BtsαI,EcoNI,TspMI,XmaI,SmaI,BtsI,BtsαI,NsiIPstI,-35 motif,-10 motif,NotI,BglII,potential RBS,BspDI,ClaI,NruI,PaeR7I,PspXI,+11

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    6182